Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cis-aconitate decarboxylase

Gene

Acod1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the inhibition of the inflammatory response. Acts as a negative regulator of the Toll-like receptors (TLRs)-mediated inflammatory innate response by stimulating the tumor necrosis factor alpha-induced protein TNFAIP3 expression via reactive oxygen species (ROS) in LPS-tolerized macrophages. Involved in antimicrobial response of innate immune cells; ACOD1-mediated itaconic acid production contributes to the antimicrobial activity of macrophages. Plays a role in the embryo implantation.3 Publications

Catalytic activityi

Cis-aconitate = itaconate + CO2.1 Publication

GO - Molecular functioni

  • aconitate decarboxylase activity Source: UniProtKB

GO - Biological processi

  • cellular response to interferon-beta Source: UniProtKB
  • cellular response to interferon-gamma Source: UniProtKB
  • cellular response to interleukin-1 Source: UniProtKB
  • cellular response to lipopolysaccharide Source: UniProtKB
  • cellular response to molecule of bacterial origin Source: UniProtKB
  • cellular response to progesterone stimulus Source: UniProtKB
  • cellular response to tumor necrosis factor Source: UniProtKB
  • defense response Source: UniProtKB
  • embryo implantation Source: UniProtKB
  • inflammatory response Source: UniProtKB-KW
  • metabolic process Source: UniProtKB
  • negative regulation of inflammatory response Source: UniProtKB
  • negative regulation of innate immune response Source: UniProtKB
  • negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
  • negative regulation of toll-like receptor 2 signaling pathway Source: UniProtKB
  • negative regulation of toll-like receptor 4 signaling pathway Source: UniProtKB
  • negative regulation of type I interferon production Source: UniProtKB
  • positive regulation of antimicrobial humoral response Source: UniProtKB
  • positive regulation of reactive oxygen species metabolic process Source: UniProtKB
  • response to lipopolysaccharide Source: MGI
  • tolerance induction to lipopolysaccharide Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Lyase

Keywords - Biological processi

Immunity, Inflammatory response, Innate immunity

Enzyme and pathway databases

BRENDAi4.1.1.6. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Cis-aconitate decarboxylase (EC:4.1.1.61 Publication)
Short name:
CAD
Alternative name(s):
Aconitate decarboxylase
Aconitate decarboxylase 1Imported
Cis-aconitic acid decarboxylase
Immune-responsive gene 1 protein
Gene namesi
Name:Acod1Imported
Synonyms:Irg1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:103206. Acod1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000842331 – 488Cis-aconitate decarboxylaseAdd BLAST488

Proteomic databases

PaxDbiP54987.
PeptideAtlasiP54987.
PRIDEiP54987.

PTM databases

iPTMnetiP54987.
PhosphoSitePlusiP54987.
SwissPalmiP54987.

Expressioni

Tissue specificityi

Expressed in LPS-tolerized macrophages (at protein level). Expressed in the luminal epithelial cells of pregnant uterus. Expressed in microglia and macrophage cells.3 Publications

Inductioni

Up-regulated after lipopolysaccharide (LPS) stimulation. Up-regulated in LPS-tolerized macrophage by LPS. Up-regulated synergistically by proinflammatory cytokines TNF and IFNG. Up-regulated by proinflammatory cytokines IL1B and IFNB1. Up-regulated by progesterone and at the time of the embryo implantation.4 Publications

Gene expression databases

BgeeiENSMUSG00000022126.
CleanExiMM_IRG1.

Interactioni

Protein-protein interaction databases

IntActiP54987. 2 interactors.
STRINGi10090.ENSMUSP00000022722.

Structurei

3D structure databases

ProteinModelPortaliP54987.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PrpD family.Curated

Phylogenomic databases

eggNOGiENOG410IEAF. Eukaryota.
COG2079. LUCA.
HOGENOMiHOG000233811.
HOVERGENiHBG059447.
InParanoidiP54987.
KOiK17724.
PhylomeDBiP54987.
TreeFamiTF332296.

Family and domain databases

InterProiIPR005656. MmgE_PrpD.
[Graphical view]
PfamiPF03972. MmgE_PrpD. 1 hit.
[Graphical view]
SUPFAMiSSF103378. SSF103378. 1 hit.

Sequencei

Sequence statusi: Complete.

P54987-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMLKSVTESF AGMIHGLKVN HLTDGIIRRS KRMILDSLGV GFLGTGTEVF
60 70 80 90 100
HKVTQYSKIY SSNTSSTVWG RPDFRLPPTY AAFVNGVAVH SMDFDDTWHP
110 120 130 140 150
ATHPSGAVLP VLTALSEALP QIPKFSGLDL LLAFNVGIEV QGRLMHFSKE
160 170 180 190 200
AKDIPKRFHP PSVVGTLGSA AAASKFLGLS LTKCREALAI AVSHAGAPIA
210 220 230 240 250
NAATQTKPLH IGNAAKHGME ATFLAMLGLQ GNKQILDLGS GFGAFYANYS
260 270 280 290 300
PEDLPSLDSH IWLLDQQDVA FKSFPAHLAT HWVADAAAAV RKHLVTPERA
310 320 330 340 350
LFPADHIERI VLRIPDVQYV NRPFPDSEHE ARHSFQYVAC ASLLDGSITV
360 370 380 390 400
PSFHSQQVNR PQVRELLKKV KLEHPPDNPP SFDTLYCEIS ITLKDGTTFT
410 420 430 440 450
ERSDTFYGHW RKPLSQEDLR NKFRANASKM LCRDTVESLI TVVEKLEDLE
460 470 480
DCSVLTRLLK GPSVQDEASK LSSMSSFDHT TLPRFTNI
Length:488
Mass (Da):53,758
Last modified:December 12, 2006 - v2
Checksum:i0F573147307DE684
GO

Sequence cautioni

The sequence AAA74554 differs from that shown. Reason: Frameshift at position 461.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti122 – 123IP → TL in BAE31070 (PubMed:16141072).Curated2
Sequence conflicti122 – 123IP → TL in BAE31377 (PubMed:16141072).Curated2
Sequence conflicti122 – 123IP → TL in BAE31131 (PubMed:16141072).Curated2
Sequence conflicti122 – 123IP → TL in BAE31008 (PubMed:16141072).Curated2
Sequence conflicti122I → T in BAE42768 (PubMed:16141072).Curated1
Sequence conflicti122I → T in BAC29433 (PubMed:16141072).Curated1
Sequence conflicti122I → T in BAE42932 (PubMed:16141072).Curated1
Sequence conflicti248N → S in BAE42768 (PubMed:16141072).Curated1
Sequence conflicti402R → G in BAE31070 (PubMed:16141072).Curated1
Sequence conflicti402R → G in BAE31377 (PubMed:16141072).Curated1
Sequence conflicti402R → G in BAE31131 (PubMed:16141072).Curated1
Sequence conflicti402R → G in BAE31008 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38281 mRNA. Translation: AAA74554.1. Sequence problems.
AK036446 mRNA. Translation: BAC29433.1.
AK152177 mRNA. Translation: BAE31008.1.
AK152248 mRNA. Translation: BAE31070.1.
AK152332 mRNA. Translation: BAE31131.1.
AK152635 mRNA. Translation: BAE31377.1.
AK172001 mRNA. Translation: BAE42768.1.
AK172298 mRNA. Translation: BAE42932.1.
CCDSiCCDS49558.1.
PIRiI54546.
RefSeqiNP_032418.1. NM_008392.1.
UniGeneiMm.4662.

Genome annotation databases

GeneIDi16365.
KEGGimmu:16365.
UCSCiuc007uwf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38281 mRNA. Translation: AAA74554.1. Sequence problems.
AK036446 mRNA. Translation: BAC29433.1.
AK152177 mRNA. Translation: BAE31008.1.
AK152248 mRNA. Translation: BAE31070.1.
AK152332 mRNA. Translation: BAE31131.1.
AK152635 mRNA. Translation: BAE31377.1.
AK172001 mRNA. Translation: BAE42768.1.
AK172298 mRNA. Translation: BAE42932.1.
CCDSiCCDS49558.1.
PIRiI54546.
RefSeqiNP_032418.1. NM_008392.1.
UniGeneiMm.4662.

3D structure databases

ProteinModelPortaliP54987.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP54987. 2 interactors.
STRINGi10090.ENSMUSP00000022722.

PTM databases

iPTMnetiP54987.
PhosphoSitePlusiP54987.
SwissPalmiP54987.

Proteomic databases

PaxDbiP54987.
PeptideAtlasiP54987.
PRIDEiP54987.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi16365.
KEGGimmu:16365.
UCSCiuc007uwf.2. mouse.

Organism-specific databases

CTDi730249.
MGIiMGI:103206. Acod1.

Phylogenomic databases

eggNOGiENOG410IEAF. Eukaryota.
COG2079. LUCA.
HOGENOMiHOG000233811.
HOVERGENiHBG059447.
InParanoidiP54987.
KOiK17724.
PhylomeDBiP54987.
TreeFamiTF332296.

Enzyme and pathway databases

BRENDAi4.1.1.6. 3474.

Miscellaneous databases

ChiTaRSiIrg1. mouse.
PROiP54987.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022126.
CleanExiMM_IRG1.

Family and domain databases

InterProiIPR005656. MmgE_PrpD.
[Graphical view]
PfamiPF03972. MmgE_PrpD. 1 hit.
[Graphical view]
SUPFAMiSSF103378. SSF103378. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiIRG1_MOUSE
AccessioniPrimary (citable) accession number: P54987
Secondary accession number(s): Q3TAA1, Q3U8E8, Q8CBA6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 12, 2006
Last modified: November 2, 2016
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.