ID   CRTB_STRGR              Reviewed;         342 AA.
AC   P54977;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   24-JAN-2024, entry version 87.
DE   RecName: Full=Phytoene synthase;
DE            Short=PSase;
DE            EC=2.5.1.-;
GN   Name=crtB; Synonyms=crtI;
OS   Streptomyces griseus.
OC   Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC   Streptomycetaceae; Streptomyces.
OX   NCBI_TaxID=1911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=JA 3933;
RX   PubMed=8917308; DOI=10.1007/bf02173971;
RA   Schumann G., Nurnberger H., Sandmann G., Kruegel H.J.;
RT   "Activation and analysis of cryptic crt genes for carotenoid biosynthesis
RT   from Streptomyces griseus.";
RL   Mol. Gen. Genet. 252:658-666(1996).
CC   -!- FUNCTION: Involved in the biosynthesis of carotenoids. Catalyzes the
CC       condensation of two molecules of geranylgeranyl diphosphate (GGPP) to
CC       give prephytoene diphosphate (PPPP) and the subsequent rearrangement of
CC       the cyclopropylcarbinyl intermediate to yield phytoene (By similarity).
CC       {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=ATP; Xref=ChEBI:CHEBI:30616; Evidence={ECO:0000250};
CC       Note=ATP is required for the transferase activity but it does not seem
CC       to be hydrolyzed during the reaction. {ECO:0000250};
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- PATHWAY: Carotenoid biosynthesis; phytoene biosynthesis.
CC   -!- SIMILARITY: Belongs to the phytoene/squalene synthase family.
CC       {ECO:0000305}.
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DR   EMBL; L37405; AAA91951.1; -; Genomic_DNA.
DR   EMBL; X95596; CAA64851.1; -; Genomic_DNA.
DR   AlphaFoldDB; P54977; -.
DR   SMR; P54977; -.
DR   STRING; 1911.GCA_001715295_00969; -.
DR   BioCyc; MetaCyc:MONOMER-20341; -.
DR   UniPathway; UPA00799; -.
DR   GO; GO:0004311; F:farnesyltranstransferase activity; IEA:InterPro.
DR   GO; GO:0016767; F:geranylgeranyl-diphosphate geranylgeranyltransferase activity; ISS:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; ISS:UniProtKB.
DR   CDD; cd00683; Trans_IPPS_HH; 1.
DR   Gene3D; 1.10.600.10; Farnesyl Diphosphate Synthase; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR002060; Squ/phyt_synthse.
DR   InterPro; IPR019845; Squalene/phytoene_synthase_CS.
DR   InterPro; IPR044843; Trans_IPPS_bact-type.
DR   InterPro; IPR033904; Trans_IPPS_HH.
DR   PANTHER; PTHR31480; BIFUNCTIONAL LYCOPENE CYCLASE/PHYTOENE SYNTHASE; 1.
DR   PANTHER; PTHR31480:SF2; PHYTOENE SYNTHASE, CHLOROPLASTIC; 1.
DR   Pfam; PF00494; SQS_PSY; 1.
DR   SFLD; SFLDG01212; Phytoene_synthase_like; 1.
DR   SFLD; SFLDG01018; Squalene/Phytoene_Synthase_Lik; 1.
DR   SUPFAM; SSF48576; Terpenoid synthases; 1.
DR   PROSITE; PS01044; SQUALEN_PHYTOEN_SYN_1; 1.
DR   PROSITE; PS01045; SQUALEN_PHYTOEN_SYN_2; 1.
PE   3: Inferred from homology;
KW   Carotenoid biosynthesis; Magnesium; Manganese; Metal-binding; Transferase.
FT   CHAIN           1..342
FT                   /note="Phytoene synthase"
FT                   /id="PRO_0000067435"
FT   REGION          321..342
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   342 AA;  37137 MW;  3685F999CBC0227C CRC64;
     MTVHELDAAG IHDPALRAAY TRCRGLNARH GKTYFLATRL LPVDRRPAVH ALYGFARWAD
     DIVDDLDTPA TPGERARALL GLEAQLEAGL RGARAAEPVI RALADTAARY TIDHRHFTDF
     LSSMRSDLTV GGYASYEELG RYMHGSAAVI GLQMLPVLGT VVAREEAAPH AAALGVAFQL
     TNFLRDVGED LDRGRLYLPT ELLAAHGADR DRFEWSRSTG MPDSRITDVL RAAAAHNRAV
     YRAALPGIAM LAPVSRPCIR TAFVLYSGIL DAIEADGYAV LHRRAVVPRR RRAAVALDGL
     VRAVAVRTVC RRRADAVDAS RSAELSRPRT AGGPAPVRRE AV
//