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Protein

Phytoene synthase

Gene

crtB

Organism
Streptomyces griseus
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of carotenoids. Catalyzes the condensation of two molecules of geranylgeranyl diphosphate (GGPP) to give prephytoene diphosphate (PPPP) and the subsequent rearrangement of the cyclopropylcarbinyl intermediate to yield phytoene (By similarity).By similarity

Cofactori

Protein has several cofactor binding sites:
  • ATPBy similarityNote: ATP is required for the transferase activity but it does not seem to be hydrolyzed during the reaction.By similarity
  • Mn2+By similarity, Mg2+By similarity

Pathway: phytoene biosynthesis

This protein is involved in the pathway phytoene biosynthesis, which is part of Carotenoid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway phytoene biosynthesis and in Carotenoid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Carotenoid biosynthesis

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00799.

Names & Taxonomyi

Protein namesi
Recommended name:
Phytoene synthase (EC:2.5.1.-)
Short name:
PSase
Gene namesi
Name:crtB
Synonyms:crtI
OrganismiStreptomyces griseus
Taxonomic identifieri1911 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 342342Phytoene synthasePRO_0000067435Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi455632.SGR_6828.

Structurei

3D structure databases

ProteinModelPortaliP54977.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phytoene/squalene synthase family.Curated

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
[Graphical view]
PfamiPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
PROSITEiPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54977-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVHELDAAG IHDPALRAAY TRCRGLNARH GKTYFLATRL LPVDRRPAVH
60 70 80 90 100
ALYGFARWAD DIVDDLDTPA TPGERARALL GLEAQLEAGL RGARAAEPVI
110 120 130 140 150
RALADTAARY TIDHRHFTDF LSSMRSDLTV GGYASYEELG RYMHGSAAVI
160 170 180 190 200
GLQMLPVLGT VVAREEAAPH AAALGVAFQL TNFLRDVGED LDRGRLYLPT
210 220 230 240 250
ELLAAHGADR DRFEWSRSTG MPDSRITDVL RAAAAHNRAV YRAALPGIAM
260 270 280 290 300
LAPVSRPCIR TAFVLYSGIL DAIEADGYAV LHRRAVVPRR RRAAVALDGL
310 320 330 340
VRAVAVRTVC RRRADAVDAS RSAELSRPRT AGGPAPVRRE AV
Length:342
Mass (Da):37,137
Last modified:October 1, 1996 - v1
Checksum:i3685F999CBC0227C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L37405 Genomic DNA. Translation: AAA91951.1.
X95596 Genomic DNA. Translation: CAA64851.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L37405 Genomic DNA. Translation: AAA91951.1.
X95596 Genomic DNA. Translation: CAA64851.1.

3D structure databases

ProteinModelPortaliP54977.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi455632.SGR_6828.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00799.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
[Graphical view]
PfamiPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
PROSITEiPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Activation and analysis of cryptic crt genes for carotenoid biosynthesis from Streptomyces griseus."
    Schumann G., Nurnberger H., Sandmann G., Kruegel H.J.
    Mol. Gen. Genet. 252:658-666(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: JA 3933.

Entry informationi

Entry nameiCRTB_STRGR
AccessioniPrimary (citable) accession number: P54977
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 24, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.