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P54975 (CRTB_PARSN) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
15-cis-phytoene synthase

Short name=PSase
EC=2.5.1.32
Gene names
Name:crtB
OrganismParacoccus sp. (strain N81106 / MBIC 01143) (Agrobacterium aurantiacum)
Taxonomic identifier81397 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeParacoccus

Protein attributes

Sequence length304 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the biosynthesis of carotenoids for the production of astaxanthin. Catalyzes the condensation of two molecules of geranylgeranyl diphosphate (GGPP) to give prephytoene diphosphate (PPPP) and the subsequent rearrangement of the cyclopropylcarbinyl intermediate to yield 15-cis phytoene. Ref.1

Catalytic activity

2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate. Ref.1

Cofactor

ATP. ATP is required for the transferase activity but it does not seem to be hydrolyzed during the reaction By similarity.

Manganese or magnesium By similarity.

Pathway

Carotenoid biosynthesis; astaxanthin biosynthesis.

Carotenoid biosynthesis; phytoene biosynthesis.

Sequence similarities

Belongs to the phytoene/squalene synthase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 30430415-cis-phytoene synthase
PRO_0000067430

Sequences

Sequence LengthMass (Da)Tools
P54975 [UniParc].

Last modified January 24, 2006. Version 2.
Checksum: A9C253BED2B90E00

FASTA30433,030
        10         20         30         40         50         60 
MSDLVLTSTE AITQGSQSFA TAAKLMPPGI RDDTVMLYAW CRHADDVIDG QALGSRPEAV 

        70         80         90        100        110        120 
NDPQARLDGL RADTLAALQG DGPVTPPFAA LRAVARRHDF PQAWPMDLIE GFAMDVEARD 

       130        140        150        160        170        180 
YRTLDDVLEY SYHVAGIVGV MMARVMGVRD DPVLDRACDL GLAFQLTNIA RDVIDDARIG 

       190        200        210        220        230        240 
RCYLPGDWLD QAGARVDGPV PSPELYTVIL RLLDAAELYY ASARVGLADL PPRCAWSIAA 

       250        260        270        280        290        300 
ALRIYRAIGL RIRKGGPEAY RQRISTSKAA KIGLLGIGGW DVARSRLPGA GVSRQGLWTR 


PHHA 

« Hide

References

[1]"Structure and functional analysis of a marine bacterial carotenoid biosynthesis gene cluster and astaxanthin biosynthetic pathway proposed at the gene level."
Misawa N., Satomi Y., Kondo K., Yokoyama A., Kajiwara S., Saito T., Ohtani T., Miki W.
J. Bacteriol. 177:6575-6584(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY.
[2]Misawa N.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 56; 90-92 AND 284-295.
[3]"Structure of the complete carotenoid biosynthesis gene cluster of Paracoccus sp. strain N81106."
Maruyama T., Inomata Y., Haga M., Ide T., Misawa N.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D58420 Genomic DNA. Translation: BAA09595.2.
AB206672 Genomic DNA. Translation: BAE47469.1.

3D structure databases

ProteinModelPortalP54975.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00387.
UPA00799.

Family and domain databases

Gene3D1.10.600.10. 1 hit.
InterProIPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMSSF48576. SSF48576. 1 hit.
PROSITEPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCRTB_PARSN
AccessionPrimary (citable) accession number: P54975
Secondary accession number(s): Q33DT6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 24, 2006
Last modified: October 16, 2013
This is version 59 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways