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P54975

- CRTB_PARSN

UniProt

P54975 - CRTB_PARSN

Protein

15-cis-phytoene synthase

Gene

crtB

Organism
Paracoccus sp. (strain N81106 / MBIC 01143) (Agrobacterium aurantiacum)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 2 (24 Jan 2006)
      Previous versions | rss
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    Functioni

    Involved in the biosynthesis of carotenoids for the production of astaxanthin. Catalyzes the condensation of two molecules of geranylgeranyl diphosphate (GGPP) to give prephytoene diphosphate (PPPP) and the subsequent rearrangement of the cyclopropylcarbinyl intermediate to yield 15-cis phytoene.1 Publication

    Catalytic activityi

    2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.1 Publication

    Cofactori

    ATP. ATP is required for the transferase activity but it does not seem to be hydrolyzed during the reaction By similarity.By similarity
    Manganese or magnesium.By similarity

    Pathwayi

    GO - Molecular functioni

    1. geranylgeranyl-diphosphate geranylgeranyltransferase activity Source: UniProtKB
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. carotenoid biosynthetic process Source: UniProtKB

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Carotenoid biosynthesis

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00387.
    UPA00799.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    15-cis-phytoene synthase (EC:2.5.1.32)
    Short name:
    PSase
    Gene namesi
    Name:crtB
    OrganismiParacoccus sp. (strain N81106 / MBIC 01143) (Agrobacterium aurantiacum)
    Taxonomic identifieri81397 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeParacoccus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 30430415-cis-phytoene synthasePRO_0000067430Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP54975.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the phytoene/squalene synthase family.Curated

    Family and domain databases

    Gene3Di1.10.600.10. 1 hit.
    InterProiIPR002060. Squ/phyt_synthse.
    IPR019845. Squalene/phytoene_synthase_CS.
    IPR008949. Terpenoid_synth.
    [Graphical view]
    PfamiPF00494. SQS_PSY. 1 hit.
    [Graphical view]
    SUPFAMiSSF48576. SSF48576. 1 hit.
    PROSITEiPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
    PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P54975-1 [UniParc]FASTAAdd to Basket

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    MSDLVLTSTE AITQGSQSFA TAAKLMPPGI RDDTVMLYAW CRHADDVIDG    50
    QALGSRPEAV NDPQARLDGL RADTLAALQG DGPVTPPFAA LRAVARRHDF 100
    PQAWPMDLIE GFAMDVEARD YRTLDDVLEY SYHVAGIVGV MMARVMGVRD 150
    DPVLDRACDL GLAFQLTNIA RDVIDDARIG RCYLPGDWLD QAGARVDGPV 200
    PSPELYTVIL RLLDAAELYY ASARVGLADL PPRCAWSIAA ALRIYRAIGL 250
    RIRKGGPEAY RQRISTSKAA KIGLLGIGGW DVARSRLPGA GVSRQGLWTR 300
    PHHA 304
    Length:304
    Mass (Da):33,030
    Last modified:January 24, 2006 - v2
    Checksum:iA9C253BED2B90E00
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D58420 Genomic DNA. Translation: BAA09595.2.
    AB206672 Genomic DNA. Translation: BAE47469.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D58420 Genomic DNA. Translation: BAA09595.2 .
    AB206672 Genomic DNA. Translation: BAE47469.1 .

    3D structure databases

    ProteinModelPortali P54975.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00387 .
    UPA00799 .

    Family and domain databases

    Gene3Di 1.10.600.10. 1 hit.
    InterProi IPR002060. Squ/phyt_synthse.
    IPR019845. Squalene/phytoene_synthase_CS.
    IPR008949. Terpenoid_synth.
    [Graphical view ]
    Pfami PF00494. SQS_PSY. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48576. SSF48576. 1 hit.
    PROSITEi PS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
    PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure and functional analysis of a marine bacterial carotenoid biosynthesis gene cluster and astaxanthin biosynthetic pathway proposed at the gene level."
      Misawa N., Satomi Y., Kondo K., Yokoyama A., Kajiwara S., Saito T., Ohtani T., Miki W.
      J. Bacteriol. 177:6575-6584(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY.
    2. Misawa N.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 56; 90-92 AND 284-295.
    3. "Structure of the complete carotenoid biosynthesis gene cluster of Paracoccus sp. strain N81106."
      Maruyama T., Inomata Y., Haga M., Ide T., Misawa N.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiCRTB_PARSN
    AccessioniPrimary (citable) accession number: P54975
    Secondary accession number(s): Q33DT6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: January 24, 2006
    Last modified: October 1, 2014
    This is version 60 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3