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Protein

IAA-amino acid hydrolase ILR1-like 2

Gene

ILL2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes certain amino acid conjugates of the plant growth regulator indole-3-acetic acid (IAA), including IAA-Ala.

Cofactori

Mn2+Note: The Mn2+ ion enhances activity.

GO - Molecular functioni

  • IAA-Ala conjugate hydrolase activity Source: TAIR
  • IAA-amino acid conjugate hydrolase activity Source: TAIR
  • metallodipeptidase activity Source: GO_Central
  • zinc ion binding Source: GO_Central

GO - Biological processi

  • auxin metabolic process Source: TAIR
  • peptide catabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Manganese

Protein family/group databases

MEROPSiM20.014.

Names & Taxonomyi

Protein namesi
Recommended name:
IAA-amino acid hydrolase ILR1-like 2 (EC:3.5.1.-)
Gene namesi
Name:ILL2
Ordered Locus Names:At5g56660
ORF Names:MIK19.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G56660.

Subcellular locationi

  • Endoplasmic reticulum lumen PROSITE-ProRule annotation

GO - Cellular componenti

  • endoplasmic reticulum Source: TAIR
  • endoplasmic reticulum lumen Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000000119022 – 439IAA-amino acid hydrolase ILR1-like 2Add BLAST418

Proteomic databases

PaxDbiP54970.

Expressioni

Gene expression databases

ExpressionAtlasiP54970. baseline and differential.
GenevisibleiP54970. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G56660.1.

Structurei

Secondary structure

1439
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi38 – 43Combined sources6
Helixi45 – 60Combined sources16
Helixi69 – 82Combined sources14
Beta strandi86 – 90Combined sources5
Turni91 – 93Combined sources3
Beta strandi94 – 104Combined sources11
Beta strandi106 – 112Combined sources7
Helixi139 – 155Combined sources17
Helixi157 – 159Combined sources3
Beta strandi161 – 169Combined sources9
Turni172 – 175Combined sources4
Helixi177 – 183Combined sources7
Turni184 – 189Combined sources6
Beta strandi190 – 203Combined sources14
Beta strandi207 – 209Combined sources3
Beta strandi211 – 215Combined sources5
Beta strandi217 – 227Combined sources11
Helixi240 – 253Combined sources14
Helixi262 – 264Combined sources3
Beta strandi266 – 274Combined sources9
Beta strandi285 – 296Combined sources12
Helixi298 – 315Combined sources18
Beta strandi318 – 325Combined sources8
Helixi326 – 328Combined sources3
Beta strandi335 – 337Combined sources3
Helixi339 – 353Combined sources15
Helixi355 – 357Combined sources3
Beta strandi358 – 360Combined sources3
Helixi370 – 374Combined sources5
Turni375 – 378Combined sources4
Beta strandi379 – 387Combined sources9
Beta strandi396 – 398Combined sources3
Helixi405 – 407Combined sources3
Helixi408 – 427Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XMBX-ray2.00A22-439[»]
2Q43X-ray2.00A22-439[»]
ProteinModelPortaliP54970.
SMRiP54970.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP54970.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi436 – 439Prevents secretion from ERPROSITE-ProRule annotation4

Sequence similaritiesi

Belongs to the peptidase M20 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IIRP. Eukaryota.
COG1473. LUCA.
HOGENOMiHOG000241403.
InParanoidiP54970.
KOiK14664.
OMAiNQMQKIA.
OrthoDBiEOG09360A3R.
PhylomeDBiP54970.

Family and domain databases

Gene3Di3.30.70.360. 1 hit.
InterProiIPR017439. Amidohydrolase.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
[Graphical view]
PfamiPF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
[Graphical view]
PIRSFiPIRSF005962. Pept_M20D_amidohydro. 1 hit.
SUPFAMiSSF55031. SSF55031. 1 hit.
TIGRFAMsiTIGR01891. amidohydrolases. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P54970-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALNKLLSLT FQLLLFLLSV SSESPWIAED TSQIQTKLLE FAKSPEVFDW
60 70 80 90 100
MVKIRRKIHE NPELGYEELE TSKLIRSELE LIGIKYRYPV AITGVIGYIG
110 120 130 140 150
TGEPPFVALR ADMDALPIQE GVEWEHKSKI AGKMHACGHD GHVTMLLGAA
160 170 180 190 200
KILHEHRHHL QGTVVLIFQP AEEGLSGAKK MREEGALKNV EAIFGIHLSA
210 220 230 240 250
RIPFGKAASR AGSFLAGAGV FEAVITGKGG HAAIPQHTID PVVAASSIVL
260 270 280 290 300
SLQQLVSRET DPLDSKVVTV SKVNGGNAFN VIPDSITIGG TLRAFTGFTQ
310 320 330 340 350
LQQRVKEVIT KQAAVHRCNA SVNLTPNGRE PMPPTVNNKD LYKQFKKVVR
360 370 380 390 400
DLLGQEAFVE AAPVMGSEDF SYFAETIPGH FSLLGMQDET NGYASSHSPL
410 420 430
YRINEDVLPY GAAIHASMAV QYLKEKASKG SVSGFHEEL
Length:439
Mass (Da):47,856
Last modified:May 27, 2002 - v2
Checksum:iBFA5F35AF4C4F508
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti131A → P in AAC49016 (PubMed:7792599).Curated1
Sequence conflicti236Q → H in AAC49016 (PubMed:7792599).Curated1
Sequence conflicti240D → G in AAL59907 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23796 mRNA. Translation: AAC49016.1.
AF047031 Genomic DNA. Translation: AAC04866.1.
AB013392 Genomic DNA. Translation: BAB09884.1.
CP002688 Genomic DNA. Translation: AED96793.1.
AY072084 mRNA. Translation: AAL59907.1.
RefSeqiNP_200477.1. NM_125049.3.
UniGeneiAt.46738.
At.66661.
At.66838.

Genome annotation databases

EnsemblPlantsiAT5G56660.1; AT5G56660.1; AT5G56660.
GeneIDi835767.
GrameneiAT5G56660.1; AT5G56660.1; AT5G56660.
KEGGiath:AT5G56660.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23796 mRNA. Translation: AAC49016.1.
AF047031 Genomic DNA. Translation: AAC04866.1.
AB013392 Genomic DNA. Translation: BAB09884.1.
CP002688 Genomic DNA. Translation: AED96793.1.
AY072084 mRNA. Translation: AAL59907.1.
RefSeqiNP_200477.1. NM_125049.3.
UniGeneiAt.46738.
At.66661.
At.66838.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XMBX-ray2.00A22-439[»]
2Q43X-ray2.00A22-439[»]
ProteinModelPortaliP54970.
SMRiP54970.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G56660.1.

Protein family/group databases

MEROPSiM20.014.

Proteomic databases

PaxDbiP54970.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G56660.1; AT5G56660.1; AT5G56660.
GeneIDi835767.
GrameneiAT5G56660.1; AT5G56660.1; AT5G56660.
KEGGiath:AT5G56660.

Organism-specific databases

TAIRiAT5G56660.

Phylogenomic databases

eggNOGiENOG410IIRP. Eukaryota.
COG1473. LUCA.
HOGENOMiHOG000241403.
InParanoidiP54970.
KOiK14664.
OMAiNQMQKIA.
OrthoDBiEOG09360A3R.
PhylomeDBiP54970.

Miscellaneous databases

EvolutionaryTraceiP54970.
PROiP54970.

Gene expression databases

ExpressionAtlasiP54970. baseline and differential.
GenevisibleiP54970. AT.

Family and domain databases

Gene3Di3.30.70.360. 1 hit.
InterProiIPR017439. Amidohydrolase.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
[Graphical view]
PfamiPF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
[Graphical view]
PIRSFiPIRSF005962. Pept_M20D_amidohydro. 1 hit.
SUPFAMiSSF55031. SSF55031. 1 hit.
TIGRFAMsiTIGR01891. amidohydrolases. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiILL2_ARATH
AccessioniPrimary (citable) accession number: P54970
Secondary accession number(s): O49221
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 27, 2002
Last modified: November 2, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.