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P54970

- ILL2_ARATH

UniProt

P54970 - ILL2_ARATH

Protein

IAA-amino acid hydrolase ILR1-like 2

Gene

ILL2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 2 (27 May 2002)
      Previous versions | rss
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    Functioni

    Hydrolyzes certain amino acid conjugates of the plant growth regulator indole-3-acetic acid (IAA), including IAA-Ala.

    Cofactori

    Manganese. The ion enhances activity.

    GO - Molecular functioni

    1. IAA-Ala conjugate hydrolase activity Source: TAIR
    2. IAA-amino acid conjugate hydrolase activity Source: TAIR

    GO - Biological processi

    1. auxin metabolic process Source: TAIR

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Manganese

    Protein family/group databases

    MEROPSiM20.014.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    IAA-amino acid hydrolase ILR1-like 2 (EC:3.5.1.-)
    Gene namesi
    Name:ILL2
    Ordered Locus Names:At5g56660
    ORF Names:MIK19.11
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G56660.

    Subcellular locationi

    Endoplasmic reticulum lumen PROSITE-ProRule annotation

    GO - Cellular componenti

    1. endoplasmic reticulum Source: TAIR
    2. endoplasmic reticulum lumen Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 439418IAA-amino acid hydrolase ILR1-like 2PRO_0000001190Add
    BLAST

    Proteomic databases

    PaxDbiP54970.
    PRIDEiP54970.

    Expressioni

    Gene expression databases

    GenevestigatoriP54970.

    Structurei

    Secondary structure

    1
    439
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi38 – 436
    Helixi45 – 6016
    Helixi69 – 8214
    Beta strandi86 – 905
    Turni91 – 933
    Beta strandi94 – 10411
    Beta strandi106 – 1127
    Helixi139 – 15517
    Helixi157 – 1593
    Beta strandi161 – 1699
    Turni172 – 1754
    Helixi177 – 1837
    Turni184 – 1896
    Beta strandi190 – 20314
    Beta strandi207 – 2093
    Beta strandi211 – 2155
    Beta strandi217 – 22711
    Helixi240 – 25314
    Helixi262 – 2643
    Beta strandi266 – 2749
    Beta strandi285 – 29612
    Helixi298 – 31518
    Beta strandi318 – 3258
    Helixi326 – 3283
    Beta strandi335 – 3373
    Helixi339 – 35315
    Helixi355 – 3573
    Beta strandi358 – 3603
    Helixi370 – 3745
    Turni375 – 3784
    Beta strandi379 – 3879
    Beta strandi396 – 3983
    Helixi405 – 4073
    Helixi408 – 42720

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1XMBX-ray2.00A22-439[»]
    2Q43X-ray2.00A22-439[»]
    ProteinModelPortaliP54970.
    SMRiP54970. Positions 37-428.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP54970.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi436 – 4394Prevents secretion from ERPROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the peptidase M20 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1473.
    HOGENOMiHOG000241403.
    InParanoidiP54970.
    KOiK14664.
    OMAiRRNLHEH.
    PhylomeDBiP54970.

    Family and domain databases

    Gene3Di3.30.70.360. 1 hit.
    InterProiIPR017439. Amidohydrolase.
    IPR002933. Peptidase_M20.
    IPR011650. Peptidase_M20_dimer.
    [Graphical view]
    PfamiPF07687. M20_dimer. 1 hit.
    PF01546. Peptidase_M20. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005962. Pept_M20D_amidohydro. 1 hit.
    SUPFAMiSSF55031. SSF55031. 1 hit.
    TIGRFAMsiTIGR01891. amidohydrolases. 1 hit.
    PROSITEiPS00014. ER_TARGET. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P54970-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALNKLLSLT FQLLLFLLSV SSESPWIAED TSQIQTKLLE FAKSPEVFDW    50
    MVKIRRKIHE NPELGYEELE TSKLIRSELE LIGIKYRYPV AITGVIGYIG 100
    TGEPPFVALR ADMDALPIQE GVEWEHKSKI AGKMHACGHD GHVTMLLGAA 150
    KILHEHRHHL QGTVVLIFQP AEEGLSGAKK MREEGALKNV EAIFGIHLSA 200
    RIPFGKAASR AGSFLAGAGV FEAVITGKGG HAAIPQHTID PVVAASSIVL 250
    SLQQLVSRET DPLDSKVVTV SKVNGGNAFN VIPDSITIGG TLRAFTGFTQ 300
    LQQRVKEVIT KQAAVHRCNA SVNLTPNGRE PMPPTVNNKD LYKQFKKVVR 350
    DLLGQEAFVE AAPVMGSEDF SYFAETIPGH FSLLGMQDET NGYASSHSPL 400
    YRINEDVLPY GAAIHASMAV QYLKEKASKG SVSGFHEEL 439
    Length:439
    Mass (Da):47,856
    Last modified:May 27, 2002 - v2
    Checksum:iBFA5F35AF4C4F508
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti131 – 1311A → P in AAC49016. (PubMed:7792599)Curated
    Sequence conflicti236 – 2361Q → H in AAC49016. (PubMed:7792599)Curated
    Sequence conflicti240 – 2401D → G in AAL59907. (PubMed:14593172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U23796 mRNA. Translation: AAC49016.1.
    AF047031 Genomic DNA. Translation: AAC04866.1.
    AB013392 Genomic DNA. Translation: BAB09884.1.
    CP002688 Genomic DNA. Translation: AED96793.1.
    AY072084 mRNA. Translation: AAL59907.1.
    RefSeqiNP_200477.1. NM_125049.3.
    UniGeneiAt.46738.
    At.66661.
    At.66838.

    Genome annotation databases

    EnsemblPlantsiAT5G56660.1; AT5G56660.1; AT5G56660.
    GeneIDi835767.
    KEGGiath:AT5G56660.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U23796 mRNA. Translation: AAC49016.1 .
    AF047031 Genomic DNA. Translation: AAC04866.1 .
    AB013392 Genomic DNA. Translation: BAB09884.1 .
    CP002688 Genomic DNA. Translation: AED96793.1 .
    AY072084 mRNA. Translation: AAL59907.1 .
    RefSeqi NP_200477.1. NM_125049.3.
    UniGenei At.46738.
    At.66661.
    At.66838.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1XMB X-ray 2.00 A 22-439 [» ]
    2Q43 X-ray 2.00 A 22-439 [» ]
    ProteinModelPortali P54970.
    SMRi P54970. Positions 37-428.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi M20.014.

    Proteomic databases

    PaxDbi P54970.
    PRIDEi P54970.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G56660.1 ; AT5G56660.1 ; AT5G56660 .
    GeneIDi 835767.
    KEGGi ath:AT5G56660.

    Organism-specific databases

    GeneFarmi 1960. 187.
    TAIRi AT5G56660.

    Phylogenomic databases

    eggNOGi COG1473.
    HOGENOMi HOG000241403.
    InParanoidi P54970.
    KOi K14664.
    OMAi RRNLHEH.
    PhylomeDBi P54970.

    Miscellaneous databases

    EvolutionaryTracei P54970.

    Gene expression databases

    Genevestigatori P54970.

    Family and domain databases

    Gene3Di 3.30.70.360. 1 hit.
    InterProi IPR017439. Amidohydrolase.
    IPR002933. Peptidase_M20.
    IPR011650. Peptidase_M20_dimer.
    [Graphical view ]
    Pfami PF07687. M20_dimer. 1 hit.
    PF01546. Peptidase_M20. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005962. Pept_M20D_amidohydro. 1 hit.
    SUPFAMi SSF55031. SSF55031. 1 hit.
    TIGRFAMsi TIGR01891. amidohydrolases. 1 hit.
    PROSITEi PS00014. ER_TARGET. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "ILR1, an amidohydrolase that releases active indole-3-acetic acid from conjugates."
      Bartel B., Fink G.R.
      Science 268:1745-1748(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Landsberg erecta.
    2. "IAR3 encodes an auxin conjugate hydrolase from Arabidopsis."
      Davies R.T., Goetz D.H., Lasswell J.E., Anderson M.N., Bartel B.
      Plant Cell 11:365-376(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Columbia.
    3. "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
      Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
      DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Characterization of a family of IAA-amino acid conjugate hydrolases from Arabidopsis."
      LeClere S., Tellez R., Rampey R.A., Matsuda S.P.T., Bartel B.
      J. Biol. Chem. 277:20446-20452(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY.
    7. "X-ray structure of IAA-aminoacid hydrolase from Arabidopsis thaliana gene At5g56660."
      Wesenberg G.E., Smith D.W., Phillips G.N. Jr., Bitto E., Bingman C.A., Allard S.T.M.
      Submitted (OCT-2004) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 22-439.

    Entry informationi

    Entry nameiILL2_ARATH
    AccessioniPrimary (citable) accession number: P54970
    Secondary accession number(s): O49221
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: May 27, 2002
    Last modified: October 1, 2014
    This is version 119 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Peptidase families
      Classification of peptidase families and list of entries
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3