P54969 (ILL1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
IAA-amino acid hydrolase ILR1-like 1
EC=3.5.1.-

Gene names

Name:	ILL1
Ordered Locus Names:	At5g56650
ORF Names:	MIK19.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	438 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes certain amino acid conjugates of the plant growth regulator indole-3-acetic acid (IAA), including IAA-Ala, IAA-Asn and IAA-Tyr.
Cofactor	Manganese. The ion enhances activity.
Subcellular location	Endoplasmic reticulum lumen Potential.
Sequence similarities	Belongs to the peptidase M20 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Signal
Ligand	Manganese
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	Golgi apparatus Inferred from direct assay PubMed 22430844. Source: TAIR endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 438	418	IAA-amino acid hydrolase ILR1-like 1		PRO_0000001189
Regions
	Motif	435 – 438	4	Prevents secretion from ER Potential
Amino acid modifications
	Modified residue	71	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P54969 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 8EAB21F1E62370B1	FASTA	438	47,692
	10 20 30 40 50 60 MALNNFLTFQ LLLLLLRVSS ESPWIVAGDV SRIPINFLEL AKSPEVFDSM VRIRRKIHEN 70 80 90 100 110 120 PELGYEEFET SKFIRSELDL IGVKYRFPVA ITGIIGYIGT GEPPFVALRA DMDALPIQEA 130 140 150 160 170 180 VEWEHKSKNP GKMHACGHDG HVAMLLGAAK ILQQHRQHLQ GTVVLIFQPA EEGLSGAKMM 190 200 210 220 230 240 REEGALKNVE AIFGIHLSPR TPFGKAASLA GSFMAGAGAF EAVITGKGGH AAIPQHTIDP 250 260 270 280 290 300 VVAASSIVLS LQHLVSRETD PSDSKVVTVT KVNGGNAFNV IPDSITIGGT LRAFTGFTQL 310 320 330 340 350 360 QERIKEIITK QAAVHRCNAS VNLAPNGNQP MPPTVNNMDL YKKFKKVVRD LLGQEAFVEA 370 380 390 400 410 420 VPEMGSEDFS YFAETIPGHF SLLGMQDETQ GYASSHSPHY RINEDVLPYG AAIHATMAVQ 430 YLKDKASKGS VSGFHDEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ILR1, an amidohydrolase that releases active indole-3-acetic acid from conjugates." Bartel B., Fink G.R. Science 268:1745-1748(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Landsberg erecta.
[2]	"IAR3 encodes an auxin conjugate hydrolase from Arabidopsis." Davies R.T., Goetz D.H., Lasswell J.E., Anderson M.N., Bartel B. Plant Cell 11:365-376(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones." Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S. DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Characterization of a family of IAA-amino acid conjugate hydrolases from Arabidopsis." LeClere S., Tellez R., Rampey R.A., Matsuda S.P.T., Bartel B. J. Biol. Chem. 277:20446-20452(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY.
[6]	"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks." Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S. Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-71, MASS SPECTROMETRY. Strain: cv. Columbia. Tissue: Seedling.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U23795 mRNA. Translation: AAC49015.1. AF047031 Genomic DNA. Translation: AAC04865.1. AB013392 Genomic DNA. Translation: BAB09883.1. CP002688 Genomic DNA. Translation: AED96792.1.
IPI	IPI00540243.
RefSeq	NP_200476.1. NM_125048.1.
UniGene	At.24009.
3D structure databases
ProteinModelPortal	P54969.
SMR	P54969. Positions 37-427.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT5G56650.1-P.
Protein family/group databases
MEROPS	M20.A03.
Proteomic databases
PaxDb	P54969.
PRIDE	P54969.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G56650.1; AT5G56650.1; AT5G56650.
GeneID	835766.
KEGG	ath:AT5G56650.
Organism-specific databases
GeneFarm	1958. 187.
TAIR	At5g56650.
Phylogenomic databases
eggNOG	COG1473.
HOGENOM	HOG000241403.
InParanoid	P54969.
KO	K14664.
OMA	TSICTAM.
PhylomeDB	P54969.
ProtClustDB	PLN02693.
Gene expression databases
Genevestigator	P54969.
GermOnline	AT5G56650. Arabidopsis thaliana.
Family and domain databases
Gene3D	3.30.70.360. 1 hit.
InterPro	IPR017439. Amidohydrolase. IPR002933. Peptidase_M20. IPR011650. Peptidase_M20_dimer. [Graphical view]
Pfam	PF07687. M20_dimer. 1 hit. PF01546. Peptidase_M20. 1 hit. [Graphical view]
PIRSF	PIRSF005962. Pept_M20D_amidohydro. 1 hit.
SUPFAM	SSF55031. Peptidase_M20_dimer. 1 hit.
TIGRFAMs	TIGR01891. amidohydrolases. 1 hit.
PROSITE	PS00014. ER_TARGET. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILL1_ARATH

Accession

Primary (citable) accession number: P54969

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 29, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program