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P54967

- BIOB_ARATH

UniProt

P54967 - BIOB_ARATH

Protein

Biotin synthase

Gene

BIO2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.By similarity
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi94 – 941Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi98 – 981Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi101 – 1011Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi138 – 1381Iron-sulfur 2 (2Fe-2S)By similarity
    Metal bindingi171 – 1711Iron-sulfur 2 (2Fe-2S)By similarity
    Metal bindingi231 – 2311Iron-sulfur 2 (2Fe-2S)By similarity
    Metal bindingi303 – 3031Iron-sulfur 2 (2Fe-2S)By similarity

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-EC
    4. zinc ion binding Source: TAIR

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthase (EC:2.8.1.6)
    Gene namesi
    Name:BIO2
    Synonyms:BIOB
    Ordered Locus Names:At2g43360
    ORF Names:T01O24.10
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G43360.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 378378Biotin synthasePRO_0000185565Add
    BLAST

    Proteomic databases

    PaxDbiP54967.
    PRIDEiP54967.

    Expressioni

    Gene expression databases

    ArrayExpressiP54967.
    GenevestigatoriP54967.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-8064668.
    STRINGi3702.AT2G43360.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliP54967.
    SMRiP54967. Positions 45-356.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239957.
    InParanoidiP54967.
    KOiK01012.
    OMAiRIMMPAS.
    PhylomeDBiP54967.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P54967-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMLVRSVFRS QLRPSVSGGL QSASCYSSLS AASAEAERTI REGPRNDWSR    50
    DEIKSVYDSP LLDLLFHGAQ VHRHVHNFRE VQQCTLLSIK TGGCSEDCSY 100
    CPQSSRYSTG VKAQRLMSKD AVIDAAKKAK EAGSTRFCMG AAWRDTIGRK 150
    TNFSQILEYI KEIRGMGMEV CCTLGMIEKQ QALELKKAGL TAYNHNLDTS 200
    REYYPNVITT RSYDDRLETL SHVRDAGINV CSGGIIGLGE AEEDRIGLLH 250
    TLATLPSHPE SVPINALLAV KGTPLEDQKP VEIWEMIRMI GTARIVMPKA 300
    MVRLSAGRVR FSMSEQALCF LAGANSIFTG EKLLTTPNND FDADQLMFKT 350
    LGLIPKPPSF SEDDSESENC EKVASASH 378
    Length:378
    Mass (Da):41,682
    Last modified:October 1, 1996 - v1
    Checksum:iB102E477E7353762
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U24147 Genomic DNA. Translation: AAA80226.1.
    U31806 mRNA. Translation: AAC49445.1.
    L34413 mRNA. Translation: AAB39953.1.
    AC002335 Genomic DNA. Translation: AAB64312.1.
    CP002685 Genomic DNA. Translation: AEC10256.1.
    PIRiS71201.
    RefSeqiNP_181864.1. NM_129897.3.
    UniGeneiAt.10203.

    Genome annotation databases

    EnsemblPlantsiAT2G43360.1; AT2G43360.1; AT2G43360.
    GeneIDi818937.
    KEGGiath:AT2G43360.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U24147 Genomic DNA. Translation: AAA80226.1 .
    U31806 mRNA. Translation: AAC49445.1 .
    L34413 mRNA. Translation: AAB39953.1 .
    AC002335 Genomic DNA. Translation: AAB64312.1 .
    CP002685 Genomic DNA. Translation: AEC10256.1 .
    PIRi S71201.
    RefSeqi NP_181864.1. NM_129897.3.
    UniGenei At.10203.

    3D structure databases

    ProteinModelPortali P54967.
    SMRi P54967. Positions 45-356.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-8064668.
    STRINGi 3702.AT2G43360.1-P.

    Proteomic databases

    PaxDbi P54967.
    PRIDEi P54967.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G43360.1 ; AT2G43360.1 ; AT2G43360 .
    GeneIDi 818937.
    KEGGi ath:AT2G43360.

    Organism-specific databases

    TAIRi AT2G43360.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239957.
    InParanoidi P54967.
    KOi K01012.
    OMAi RIMMPAS.
    PhylomeDBi P54967.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .

    Miscellaneous databases

    PROi P54967.

    Gene expression databases

    ArrayExpressi P54967.
    Genevestigatori P54967.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the cDNA and gene coding for the biotin synthase of Arabidopsis thaliana."
      Weaver L.M., Yu F., Wurtele E.S., Nikolau B.J.
      Plant Physiol. 110:1021-1028(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Strain: cv. Landsberg erecta.
    2. "Biotin synthase from Arabidopsis thaliana. cDNA isolation and characterization of gene expression."
      Patton D.A., Johnson M., Ward E.R.
      Plant Physiol. 112:371-378(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
      Tissue: Leaf.
    3. "Biotin synthesis in higher plants: isolation of a cDNA encoding Arabidopsis thaliana bioB-gene product equivalent by functional complementation of a biotin auxotroph mutant bioB105 of Escherichia coli K12."
      Baldet P., Ruffet M.L.
      C. R. Acad. Sci. III, Sci. Vie 319:99-106(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
      Tissue: Leaf.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    5. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiBIOB_ARATH
    AccessioniPrimary (citable) accession number: P54967
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3