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Protein

Biotin synthase, mitochondrial

Gene

BIO2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity
  • [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.By similarity

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Bifunctional dethiobiotin synthetase/7,8-diamino-pelargonic acid aminotransferase, mitochondrial (BIO3-BIO1)
  2. Biotin synthase, mitochondrial (BIO2)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi94Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi98Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi101Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi138Iron-sulfur 2 (2Fe-2S)By similarity1
Metal bindingi171Iron-sulfur 2 (2Fe-2S)By similarity1
Metal bindingi231Iron-sulfur 2 (2Fe-2S)By similarity1
Metal bindingi303Iron-sulfur 2 (2Fe-2S)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processBiotin biosynthesis
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00078; UER00162

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase, mitochondrial (EC:2.8.1.6)
Gene namesi
Name:BIO2
Synonyms:BIOB
Ordered Locus Names:At2g43360
ORF Names:T01O24.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G43360
TAIRilocus:2005497 AT2G43360

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 26Mitochondrion1 PublicationAdd BLAST26
ChainiPRO_000018556527 – 378Biotin synthase, mitochondrialAdd BLAST352

Proteomic databases

PaxDbiP54967

PTM databases

iPTMnetiP54967

Expressioni

Gene expression databases

ExpressionAtlasiP54967 baseline and differential
GenevisibleiP54967 AT

Interactioni

Protein-protein interaction databases

BioGridi4274, 1 interactor
STRINGi3702.AT2G43360.1

Structurei

3D structure databases

ProteinModelPortaliP54967
SMRiP54967
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2900 Eukaryota
COG0502 LUCA
HOGENOMiHOG000239957
InParanoidiP54967
KOiK01012
OMAiADRFCMG
OrthoDBiEOG09360F2C
PhylomeDBiP54967

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01694 BioB, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR010722 BATS_dom
IPR034416 BATS_domain_containing
IPR002684 Biotin_synth/BioAB
IPR024177 Biotin_synthase
IPR006638 Elp3/MiaB/NifB
IPR007197 rSAM
PANTHERiPTHR22976 PTHR22976, 1 hit
PfamiView protein in Pfam
PF06968 BATS, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF001619 Biotin_synth, 1 hit
SFLDiSFLDF00272 biotin_synthase, 1 hit
SFLDG01060 BATS_domain_containing, 1 hit
SFLDG01278 biotin_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00876 BATS, 1 hit
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00433 bioB, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P54967-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMLVRSVFRS QLRPSVSGGL QSASCYSSLS AASAEAERTI REGPRNDWSR
60 70 80 90 100
DEIKSVYDSP LLDLLFHGAQ VHRHVHNFRE VQQCTLLSIK TGGCSEDCSY
110 120 130 140 150
CPQSSRYSTG VKAQRLMSKD AVIDAAKKAK EAGSTRFCMG AAWRDTIGRK
160 170 180 190 200
TNFSQILEYI KEIRGMGMEV CCTLGMIEKQ QALELKKAGL TAYNHNLDTS
210 220 230 240 250
REYYPNVITT RSYDDRLETL SHVRDAGINV CSGGIIGLGE AEEDRIGLLH
260 270 280 290 300
TLATLPSHPE SVPINALLAV KGTPLEDQKP VEIWEMIRMI GTARIVMPKA
310 320 330 340 350
MVRLSAGRVR FSMSEQALCF LAGANSIFTG EKLLTTPNND FDADQLMFKT
360 370
LGLIPKPPSF SEDDSESENC EKVASASH
Length:378
Mass (Da):41,682
Last modified:October 1, 1996 - v1
Checksum:iB102E477E7353762
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U24147 Genomic DNA Translation: AAA80226.1
U31806 mRNA Translation: AAC49445.1
L34413 mRNA Translation: AAB39953.1
AC002335 Genomic DNA Translation: AAB64312.1
CP002685 Genomic DNA Translation: AEC10256.1
PIRiS71201
RefSeqiNP_181864.1, NM_129897.4
UniGeneiAt.10203

Genome annotation databases

EnsemblPlantsiAT2G43360.1; AT2G43360.1; AT2G43360
GeneIDi818937
GrameneiAT2G43360.1; AT2G43360.1; AT2G43360
KEGGiath:AT2G43360

Similar proteinsi

Entry informationi

Entry nameiBIOB_ARATH
AccessioniPrimary (citable) accession number: P54967
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 25, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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