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P54967

- BIOB_ARATH

UniProt

P54967 - BIOB_ARATH

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Protein

Biotin synthase

Gene

BIO2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity
  • [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi94 – 941Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity
Metal bindingi98 – 981Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity
Metal bindingi101 – 1011Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity
Metal bindingi138 – 1381Iron-sulfur 2 (2Fe-2S)By similarity
Metal bindingi171 – 1711Iron-sulfur 2 (2Fe-2S)By similarity
Metal bindingi231 – 2311Iron-sulfur 2 (2Fe-2S)By similarity
Metal bindingi303 – 3031Iron-sulfur 2 (2Fe-2S)By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-EC
  4. zinc ion binding Source: TAIR

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:BIO2
Synonyms:BIOB
Ordered Locus Names:At2g43360
ORF Names:T01O24.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G43360.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 378378Biotin synthasePRO_0000185565Add
BLAST

Proteomic databases

PaxDbiP54967.
PRIDEiP54967.

Expressioni

Gene expression databases

ExpressionAtlasiP54967. baseline and differential.
GenevestigatoriP54967.

Interactioni

Protein-protein interaction databases

MINTiMINT-8064668.
STRINGi3702.AT2G43360.1-P.

Structurei

3D structure databases

ProteinModelPortaliP54967.
SMRiP54967. Positions 45-356.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
InParanoidiP54967.
KOiK01012.
OMAiRIMMPAS.
PhylomeDBiP54967.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

P54967-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMLVRSVFRS QLRPSVSGGL QSASCYSSLS AASAEAERTI REGPRNDWSR
60 70 80 90 100
DEIKSVYDSP LLDLLFHGAQ VHRHVHNFRE VQQCTLLSIK TGGCSEDCSY
110 120 130 140 150
CPQSSRYSTG VKAQRLMSKD AVIDAAKKAK EAGSTRFCMG AAWRDTIGRK
160 170 180 190 200
TNFSQILEYI KEIRGMGMEV CCTLGMIEKQ QALELKKAGL TAYNHNLDTS
210 220 230 240 250
REYYPNVITT RSYDDRLETL SHVRDAGINV CSGGIIGLGE AEEDRIGLLH
260 270 280 290 300
TLATLPSHPE SVPINALLAV KGTPLEDQKP VEIWEMIRMI GTARIVMPKA
310 320 330 340 350
MVRLSAGRVR FSMSEQALCF LAGANSIFTG EKLLTTPNND FDADQLMFKT
360 370
LGLIPKPPSF SEDDSESENC EKVASASH
Length:378
Mass (Da):41,682
Last modified:October 1, 1996 - v1
Checksum:iB102E477E7353762
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U24147 Genomic DNA. Translation: AAA80226.1.
U31806 mRNA. Translation: AAC49445.1.
L34413 mRNA. Translation: AAB39953.1.
AC002335 Genomic DNA. Translation: AAB64312.1.
CP002685 Genomic DNA. Translation: AEC10256.1.
PIRiS71201.
RefSeqiNP_181864.1. NM_129897.3.
UniGeneiAt.10203.

Genome annotation databases

EnsemblPlantsiAT2G43360.1; AT2G43360.1; AT2G43360.
GeneIDi818937.
KEGGiath:AT2G43360.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U24147 Genomic DNA. Translation: AAA80226.1 .
U31806 mRNA. Translation: AAC49445.1 .
L34413 mRNA. Translation: AAB39953.1 .
AC002335 Genomic DNA. Translation: AAB64312.1 .
CP002685 Genomic DNA. Translation: AEC10256.1 .
PIRi S71201.
RefSeqi NP_181864.1. NM_129897.3.
UniGenei At.10203.

3D structure databases

ProteinModelPortali P54967.
SMRi P54967. Positions 45-356.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-8064668.
STRINGi 3702.AT2G43360.1-P.

Proteomic databases

PaxDbi P54967.
PRIDEi P54967.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G43360.1 ; AT2G43360.1 ; AT2G43360 .
GeneIDi 818937.
KEGGi ath:AT2G43360.

Organism-specific databases

TAIRi AT2G43360.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
InParanoidi P54967.
KOi K01012.
OMAi RIMMPAS.
PhylomeDBi P54967.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .

Miscellaneous databases

PROi P54967.

Gene expression databases

ExpressionAtlasi P54967. baseline and differential.
Genevestigatori P54967.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the cDNA and gene coding for the biotin synthase of Arabidopsis thaliana."
    Weaver L.M., Yu F., Wurtele E.S., Nikolau B.J.
    Plant Physiol. 110:1021-1028(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: cv. Landsberg erecta.
  2. "Biotin synthase from Arabidopsis thaliana. cDNA isolation and characterization of gene expression."
    Patton D.A., Johnson M., Ward E.R.
    Plant Physiol. 112:371-378(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
    Tissue: Leaf.
  3. "Biotin synthesis in higher plants: isolation of a cDNA encoding Arabidopsis thaliana bioB-gene product equivalent by functional complementation of a biotin auxotroph mutant bioB105 of Escherichia coli K12."
    Baldet P., Ruffet M.L.
    C. R. Acad. Sci. III, Sci. Vie 319:99-106(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
    Tissue: Leaf.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  5. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.

Entry informationi

Entry nameiBIOB_ARATH
AccessioniPrimary (citable) accession number: P54967
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 26, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3