P54967 (BIOB_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Biotin synthase
EC=2.8.1.6

Gene names

Name:	BIO2
Synonyms:	BIOB
Ordered Locus Names:	At2g43360
ORF Names:	T01O24.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Dethiobiotin + sulfur + 2 S-adenosyl-L-methionine = biotin + 2 L-methionine + 2 5'-deoxyadenosine.
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity. Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2.
Sequence similarities	Belongs to the radical SAM superfamily. Biotin synthase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW biotin synthase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from direct assay PubMed 20018591. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 378	378	Biotin synthase		PRO_0000185565
Sites
	Metal binding	94	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	98	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	101	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	138	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	171	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	231	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	303	1	Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P54967 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: B102E477E7353762	FASTA	378	41,682
	10 20 30 40 50 60 MMLVRSVFRS QLRPSVSGGL QSASCYSSLS AASAEAERTI REGPRNDWSR DEIKSVYDSP 70 80 90 100 110 120 LLDLLFHGAQ VHRHVHNFRE VQQCTLLSIK TGGCSEDCSY CPQSSRYSTG VKAQRLMSKD 130 140 150 160 170 180 AVIDAAKKAK EAGSTRFCMG AAWRDTIGRK TNFSQILEYI KEIRGMGMEV CCTLGMIEKQ 190 200 210 220 230 240 QALELKKAGL TAYNHNLDTS REYYPNVITT RSYDDRLETL SHVRDAGINV CSGGIIGLGE 250 260 270 280 290 300 AEEDRIGLLH TLATLPSHPE SVPINALLAV KGTPLEDQKP VEIWEMIRMI GTARIVMPKA 310 320 330 340 350 360 MVRLSAGRVR FSMSEQALCF LAGANSIFTG EKLLTTPNND FDADQLMFKT LGLIPKPPSF 370 SEDDSESENC EKVASASH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the cDNA and gene coding for the biotin synthase of Arabidopsis thaliana." Weaver L.M., Yu F., Wurtele E.S., Nikolau B.J. Plant Physiol. 110:1021-1028(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: cv. Landsberg erecta.
[2]	"Biotin synthase from Arabidopsis thaliana. cDNA isolation and characterization of gene expression." Patton D.A., Johnson M., Ward E.R. Plant Physiol. 112:371-378(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. Tissue: Leaf.
[3]	"Biotin synthesis in higher plants: isolation of a cDNA encoding Arabidopsis thaliana bioB-gene product equivalent by functional complementation of a biotin auxotroph mutant bioB105 of Escherichia coli K12." Baldet P., Ruffet M.L. C. R. Acad. Sci. III, Sci. Vie 319:99-106(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. Tissue: Leaf.
[4]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[5]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U24147 Genomic DNA. Translation: AAA80226.1. U31806 mRNA. Translation: AAC49445.1. L34413 mRNA. Translation: AAB39953.1. AC002335 Genomic DNA. Translation: AAB64312.1. CP002685 Genomic DNA. Translation: AEC10256.1.
IPI	IPI00517122.
PIR	S71201.
RefSeq	NP_181864.1. NM_129897.3.
UniGene	At.10203.
3D structure databases
ProteinModelPortal	P54967.
SMR	P54967. Positions 45-356.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT2G43360.1-P.
Proteomic databases
PaxDb	P54967.
PRIDE	P54967.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G43360.1; AT2G43360.1; AT2G43360.
GeneID	818937.
KEGG	ath:AT2G43360.
Organism-specific databases
TAIR	At2g43360.
Phylogenomic databases
eggNOG	COG0502.
HOGENOM	HOG000239957.
InParanoid	P54967.
KO	K01012.
OMA	GVEVCAC.
PhylomeDB	P54967.
ProtClustDB	PLN02389.
Enzyme and pathway databases
UniPathway	UPA00078; UER00162.
Gene expression databases
ArrayExpress	P54967.
Genevestigator	P54967.
GermOnline	AT2G43360. Arabidopsis thaliana.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR010722. Biotin/thiamin_synth-assoc. IPR002684. Biotin_synth/BioAB. IPR024177. Biotin_synthase. IPR006638. Elp3/MiaB/NifB. IPR007197. rSAM. [Graphical view]
Pfam	PF06968. BATS. 1 hit. PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF001619. Biotin_synth. 1 hit.
SMART	SM00876. BATS. 1 hit. SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00433. bioB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOB_ARATH

Accession

Primary (citable) accession number: P54967

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program