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P54967 (BIOB_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:BIO2
Synonyms:BIOB
Ordered Locus Names:At2g43360
ORF Names:T01O24.10
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length378 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 378378Biotin synthase HAMAP-Rule MF_01694
PRO_0000185565

Sites

Metal binding941Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding981Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1011Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1381Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1711Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2311Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding3031Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
P54967 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: B102E477E7353762

FASTA37841,682
        10         20         30         40         50         60 
MMLVRSVFRS QLRPSVSGGL QSASCYSSLS AASAEAERTI REGPRNDWSR DEIKSVYDSP 

        70         80         90        100        110        120 
LLDLLFHGAQ VHRHVHNFRE VQQCTLLSIK TGGCSEDCSY CPQSSRYSTG VKAQRLMSKD 

       130        140        150        160        170        180 
AVIDAAKKAK EAGSTRFCMG AAWRDTIGRK TNFSQILEYI KEIRGMGMEV CCTLGMIEKQ 

       190        200        210        220        230        240 
QALELKKAGL TAYNHNLDTS REYYPNVITT RSYDDRLETL SHVRDAGINV CSGGIIGLGE 

       250        260        270        280        290        300 
AEEDRIGLLH TLATLPSHPE SVPINALLAV KGTPLEDQKP VEIWEMIRMI GTARIVMPKA 

       310        320        330        340        350        360 
MVRLSAGRVR FSMSEQALCF LAGANSIFTG EKLLTTPNND FDADQLMFKT LGLIPKPPSF 

       370 
SEDDSESENC EKVASASH 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of the cDNA and gene coding for the biotin synthase of Arabidopsis thaliana."
Weaver L.M., Yu F., Wurtele E.S., Nikolau B.J.
Plant Physiol. 110:1021-1028(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: cv. Landsberg erecta.
[2]"Biotin synthase from Arabidopsis thaliana. cDNA isolation and characterization of gene expression."
Patton D.A., Johnson M., Ward E.R.
Plant Physiol. 112:371-378(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
Tissue: Leaf.
[3]"Biotin synthesis in higher plants: isolation of a cDNA encoding Arabidopsis thaliana bioB-gene product equivalent by functional complementation of a biotin auxotroph mutant bioB105 of Escherichia coli K12."
Baldet P., Ruffet M.L.
C. R. Acad. Sci. III, Sci. Vie 319:99-106(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
Tissue: Leaf.
[4]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[5]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U24147 Genomic DNA. Translation: AAA80226.1.
U31806 mRNA. Translation: AAC49445.1.
L34413 mRNA. Translation: AAB39953.1.
AC002335 Genomic DNA. Translation: AAB64312.1.
CP002685 Genomic DNA. Translation: AEC10256.1.
PIRS71201.
RefSeqNP_181864.1. NM_129897.3.
UniGeneAt.10203.

3D structure databases

ProteinModelPortalP54967.
SMRP54967. Positions 45-356.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-8064668.
STRING3702.AT2G43360.1-P.

Proteomic databases

PaxDbP54967.
PRIDEP54967.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G43360.1; AT2G43360.1; AT2G43360.
GeneID818937.
KEGGath:AT2G43360.

Organism-specific databases

TAIRAT2G43360.

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
InParanoidP54967.
KOK01012.
OMARIMMPAS.
PhylomeDBP54967.

Enzyme and pathway databases

UniPathwayUPA00078; UER00162.

Gene expression databases

ArrayExpressP54967.
GenevestigatorP54967.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Other

PROP54967.

Entry information

Entry nameBIOB_ARATH
AccessionPrimary (citable) accession number: P54967
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 14, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names