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Protein

Choloylglycine hydrolase

Gene

cbh

Organism
Clostridium perfringens (strain 13 / Type A)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The enzyme catalyzes the degradation of conjugated bile acids in the mammalian gut.

Catalytic activityi

3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholan-24-oylglycine + H2O = 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholanate + glycine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei21

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15681.
BRENDAi3.5.1.24. 1503.
SABIO-RKP54965.

Names & Taxonomyi

Protein namesi
Recommended name:
Choloylglycine hydrolase (EC:3.5.1.24)
Alternative name(s):
Bile salt hydrolase
Conjugated bile acid hydrolase
Short name:
CBAH
Gene namesi
Name:cbh
Ordered Locus Names:CPE0709
OrganismiClostridium perfringens (strain 13 / Type A)
Taxonomic identifieri195102 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000000818 Componenti: Chromosome

Pathology & Biotechi

Chemistry databases

DrugBankiDB02659. Cholic Acid.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00000730192 – 329Choloylglycine hydrolaseAdd BLAST328

Interactioni

Subunit structurei

Homotetramer.1 Publication

Structurei

Secondary structure

1329
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Beta strandi14 – 24Combined sources11
Beta strandi29 – 33Combined sources5
Beta strandi38 – 40Combined sources3
Turni42 – 44Combined sources3
Beta strandi47 – 49Combined sources3
Beta strandi54 – 61Combined sources8
Beta strandi64 – 72Combined sources9
Beta strandi77 – 82Combined sources6
Turni84 – 86Combined sources3
Beta strandi90 – 92Combined sources3
Beta strandi97 – 101Combined sources5
Helixi102 – 104Combined sources3
Helixi105 – 112Combined sources8
Helixi116 – 123Combined sources8
Beta strandi126 – 129Combined sources4
Beta strandi142 – 147Combined sources6
Beta strandi153 – 158Combined sources6
Beta strandi163 – 167Combined sources5
Beta strandi170 – 173Combined sources4
Beta strandi175 – 177Combined sources3
Helixi179 – 186Combined sources8
Helixi187 – 189Combined sources3
Beta strandi199 – 202Combined sources4
Beta strandi205 – 208Combined sources4
Beta strandi210 – 212Combined sources3
Helixi214 – 216Combined sources3
Helixi225 – 243Combined sources19
Helixi244 – 246Combined sources3
Helixi249 – 257Combined sources9
Turni263 – 265Combined sources3
Beta strandi275 – 283Combined sources9
Turni284 – 287Combined sources4
Beta strandi288 – 295Combined sources8
Beta strandi300 – 303Combined sources4
Helixi304 – 306Combined sources3
Beta strandi315 – 318Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BJFX-ray1.67A1-329[»]
2BJGX-ray2.10A/B1-329[»]
2RF8X-ray2.90A/B1-329[»]
2RG2X-ray1.80A2-329[»]
2RLCX-ray1.80A2-329[»]
ProteinModelPortaliP54965.
SMRiP54965.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP54965.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C59 family.Curated

Phylogenomic databases

HOGENOMiHOG000039938.
KOiK01442.
OMAiHYAIIGI.
OrthoDBiPOG091H062W.

Family and domain databases

Gene3Di3.60.60.10. 1 hit.
InterProiIPR029132. CBAH/NAAA_C.
IPR003199. Chologlycine_hydro/PeptC59.
IPR029055. Ntn_hydrolases_N.
[Graphical view]
PfamiPF02275. CBAH. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P54965-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCTGLALETK DGLHLFGRNM DIEYSFNQSI IFIPRNFKCV NKSNKKELTT
60 70 80 90 100
KYAVLGMGTI FDDYPTFADG MNEKGLGCAG LNFPVYVSYS KEDIEGKTNI
110 120 130 140 150
PVYNFLLWVL ANFSSVEEVK EALKNANIVD IPISENIPNT TLHWMISDIT
160 170 180 190 200
GKSIVVEQTK EKLNVFDNNI GVLTNSPTFD WHVANLNQYV GLRYNQVPEF
210 220 230 240 250
KLGDQSLTAL GQGTGLVGLP GDFTPASRFI RVAFLRDAMI KNDKDSIDLI
260 270 280 290 300
EFFHILNNVA MVRGSTRTVE EKSDLTQYTS CMCLEKGIYY YNTYENNQIN
310 320
AIDMNKENLD GNEIKTYKYN KTLSINHVN
Length:329
Mass (Da):37,185
Last modified:January 23, 2007 - v3
Checksum:iB0643160A27A368D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20191 Genomic DNA. Translation: AAC43454.1.
BA000016 Genomic DNA. Translation: BAB80415.1.
PIRiI40881.
RefSeqiWP_003461725.1. NC_003366.1.

Genome annotation databases

EnsemblBacteriaiBAB80415; BAB80415; BAB80415.
KEGGicpe:CPE0709.
PATRICi19495345. VBICloPer59675_0771.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20191 Genomic DNA. Translation: AAC43454.1.
BA000016 Genomic DNA. Translation: BAB80415.1.
PIRiI40881.
RefSeqiWP_003461725.1. NC_003366.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BJFX-ray1.67A1-329[»]
2BJGX-ray2.10A/B1-329[»]
2RF8X-ray2.90A/B1-329[»]
2RG2X-ray1.80A2-329[»]
2RLCX-ray1.80A2-329[»]
ProteinModelPortaliP54965.
SMRiP54965.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

DrugBankiDB02659. Cholic Acid.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB80415; BAB80415; BAB80415.
KEGGicpe:CPE0709.
PATRICi19495345. VBICloPer59675_0771.

Phylogenomic databases

HOGENOMiHOG000039938.
KOiK01442.
OMAiHYAIIGI.
OrthoDBiPOG091H062W.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15681.
BRENDAi3.5.1.24. 1503.
SABIO-RKP54965.

Miscellaneous databases

EvolutionaryTraceiP54965.

Family and domain databases

Gene3Di3.60.60.10. 1 hit.
InterProiIPR029132. CBAH/NAAA_C.
IPR003199. Chologlycine_hydro/PeptC59.
IPR029055. Ntn_hydrolases_N.
[Graphical view]
PfamiPF02275. CBAH. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCBH_CLOPE
AccessioniPrimary (citable) accession number: P54965
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.