P54964 (ORN_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Oligoribonuclease, mitochondrial
EC=3.1.-.-

Gene names

Name:	REX2
Synonyms:	YNT20
Ordered Locus Names:	YLR059C
ORF Names:	L2159

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	3'-to-5' exoribonuclease specific for small oligoribonucleotides By similarity.
Subcellular location	Mitochondrion Ref.5.
Miscellaneous	Present with 1730 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the oligoribonuclease family. Contains 1 exonuclease domain.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Exonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	U4 snRNA 3'-end processing Inferred from mutant phenotype. Source: SGD mitochondrial genome maintenance Inferred from mutant phenotype Ref.5. Source: SGD
Cellular component	mitochondrion Inferred from direct assay Ref.5. Source: SGD
Molecular function	3'-5'-exoribonuclease activity Inferred from mutant phenotype. Source: SGD nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
JSN1	P47135	1	EBI-12596,EBI-9422

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 269		Oligoribonuclease, mitochondrial		PRO_0000020274
Regions
	Domain	55 – 227	173	Exonuclease
Sites
	Active site	184	1	Potential
Experimental info
	Sequence conflict	100	1	S → T in AAA98633. Ref.1
	Sequence conflict	124 – 127	4	GLTA → ESHP in AAA98633. Ref.1
	Sequence conflict	181	1	H → R in AAT93024. Ref.4
	Sequence conflict	223 – 269	47	SIAQL…QRTDF → HSSIAMVHGQLLEATTGNRV GRVNRI Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P54964 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: C942C823691E9815	FASTA	269	30,870
	10 20 30 40 50 60 MKWLLFPARI VARTRPNLFS LYRRSVSQYL RPRTIQNLQS MAQTPELKTK LFKPLVWIDC 70 80 90 100 110 120 EMTGLDHVND RIIEICCIIT DGHLAPVKAA DGQGDSHYES VIHYGPEVMN KMNEWCIEHH 130 140 150 160 170 180 GNSGLTAKVL ASEKTLAQVE DELLEYIQRY IPDKNVGVLA GNSVHMDRLF MVREFPKVID 190 200 210 220 230 240 HLFYRIVDVS SIMEVARRHN PALQARNPKK EAAHTAYSDI KESIAQLQWY MDNYLKPPQE 250 260 TESVESIGSE QPESPSSSTS SLKRQRTDF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Hanekamp T., Thorsness P.E. Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"YNT20, a bypass suppressor of yme1 yme2, encodes a putative 3'-5' exonuclease localized in mitochondria of Saccharomyces cerevisiae." Hanekamp T., Thorsness P.E. Curr. Genet. 34:438-448(1999) [PubMed: 9933355] [Abstract] Cited for: IDENTIFICATION, SUBCELLULAR LOCATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L47977 Genomic DNA. Translation: AAA98633.1. X94607 Genomic DNA. Translation: CAA64306.1. Z73231 Genomic DNA. Translation: CAA97590.1. AY693005 Genomic DNA. Translation: AAT93024.1. BK006945 Genomic DNA. Translation: DAA09377.1.
PIR	S61633.
RefSeq	NP_013160.1. NM_001181946.1.
3D structure databases
ProteinModelPortal	P54964.
SMR	P54964. Positions 53-239.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5084N.
IntAct	P54964. 6 interactions.
MINT	MINT-549084.
STRING	P54964.
Proteomic databases
PeptideAtlas	P54964.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR059C; YLR059C; YLR059C.
GeneID	850748.
KEGG	sce:YLR059C.
NMPDR	fig|4932.3.peg.4151.
Organism-specific databases
CYGD	YLR059c.
SGD	S000004049. REX2.
Phylogenomic databases
eggNOG	fuNOG08943.
HOGENOM	HBG448545.
OMA	RIIEICC.
OrthoDB	EOG43R6X0.
Gene expression databases
ArrayExpress	P54964.
Genevestigator	P54964.
GermOnline	YLR059C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006055. Exonuclease. IPR013520. Exonuclease_RNaseT/DNA_pol3. IPR012337. RNaseH-like_dom. [Graphical view]
KO	K13288.
Pfam	PF00929. RNase_T. 1 hit. [Graphical view]
SMART	SM00479. EXOIII. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
ProtoNet	Search...
Other
NextBio	966876.

Entry information

Entry name

ORN_YEAST

Accession

Primary (citable) accession number: P54964
Secondary accession number(s): D6VY61, E9P909

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program