Reviewed, UniProtKB/Swiss-Prot P54960 (HMDH_BLAGE)

Names and origin

Protein names	Recommended name:     3-hydroxy-3-methylglutaryl-coenzyme A reductase       Short name=HMG-CoA reductase     EC=1.1.1.34
Organism	Blattella germanica (German cockroach)
Taxonomic identifier	6973 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Orthopteroidea › Dictyoptera › Blattaria › Blattellidae › Blattellinae › Blattella

Protein attributes

Sequence length	856 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Synthesis of mevalonate for the production of non-sterol isoprenoids, which are essential for growth differentiation.
Catalytic activity	(R)-mevalonate + CoA + 2 NADP(+) = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
Enzyme regulation	The activity of HMG-CoA-reductase is suppressed by exogenous mevalonate.
Pathway	Metabolic intermediate biosynthesis; mevalonic acid biosynthesis; (R)-mevalonic acid from acetyl-CoA: step 3/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the HMG-CoA reductase family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	coenzyme A metabolic process Inferred from electronic annotation. Source: InterPro isoprenoid biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: InterPro
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 856	856	3-hydroxy-3-methylglutaryl-coenzyme A reductase		PRO_0000114430
Regions
	Transmembrane	12 – 32	21	Potential
	Transmembrane	89 – 109	21	Potential
	Transmembrane	123 – 143	21	Potential
	Transmembrane	190 – 210	21	Potential
	Transmembrane	344 – 364	21	Potential
	Region	365 – 443	79	Linker
	Region	443 – 771	329	Catalytic
Sites
	Active site	528	1	Charge relay system By similarity
	Active site	659	1	Charge relay system By similarity
	Active site	735	1	Charge relay system By similarity
	Active site	834	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	326	1	N-linked (GlcNAc...) Potential
	Glycosylation	412	1	N-linked (GlcNAc...) Potential
	Glycosylation	700	1	N-linked (GlcNAc...) Potential
	Glycosylation	838	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P54960-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 833273836FC55AAA	FASTA	856	93,157
	10 20 30 40 50 60 MVGRLFRAHG QFCASHPWEV IVATLTLTVC MLTVDQRPLG LPPGWGHNCI TLEEYNAADM 70 80 90 100 110 120 IVMTLIRCVA VLYSYYQFCH LQKLGSKYIL GIAGLFTVFS SFVFSSSVIN FLGSDVSDLK 130 140 150 160 170 180 DALFFFLLLI DLSKATVLAQ FALSSRSQDE VKHNIARGIA MLGPTITLDT VVETLVIGVG 190 200 210 220 230 240 MLSGVRRLEV LCCFACMSVI VNYVVFMTFY PACLSLILEL SRSGESGRPA WHDKSLIIKA 250 260 270 280 290 300 LHEEDQKPNP VVQRVKVIMS AGLMLVHAHR WVRCLSIALW PDLTSLRYFC THCDTGVSYS 310 320 330 340 350 360 RWSFASEGEE LPTVKLVTGD SVVNSNSTDD AQLHYYIMRW LTVSADHIVI LILLLALAVK 370 380 390 400 410 420 FVFFETRDEL TTTRGMDGWV EVSSPVEHKY VQTEQPSCSA PEQPLEEPPA SNRSIDECLS 430 440 450 460 470 480 VCKSDVGAQA LSDCEVMALV TSGHIAGYQL EKVVRNPERG VGIRRQILTK TADLKDALDN 490 500 510 520 530 540 LPYKNYDYLK VMGACCENVI GYMPVPVGVA GPLNLDGRLV HVPLATTEGC LVASTNRGMR 550 560 570 580 590 600 ALMRCGVTSR IVADGMTRGP VVRFPNIDRA SEAMLWMQVP YNFEQIKKNF DSTSRFARLS 610 620 630 640 650 660 KIHIRVAGRH LFIRFIATTG DAMGMNMLSK GTEVALAYVQ QVYPDMEILS LSGNFCTDKK 670 680 690 700 710 720 PAAVNWIEGR GKSVVCEAIV PADIIKSVLK TSVQALMDVN ITKNLIGSAV AGSIGGFNAH 730 740 750 760 770 780 AANIVTAIFI ATGQDPAQNV GSSNCMTLME PWGEDGKDLY VSCTMPSIEI GTIGGGTVLP 790 800 810 820 830 840 PQAACLDMLG VRGANEMCPG ENANTLARIV CGTVLAGELS LMSALAAGHL VKSHMRHNRS 850 SVSTSGSEPS TPACKS

References

[1]

"Molecular cloning, developmental pattern and tissue expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase of the cockroach Blattella germanica." Martinez-Gonzalez J., Buesa C., Piulachs M.D., Belles X., Hegardt F.G. Eur. J. Biochem. 213:233-241(1993) [PubMed: 8477698] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X70034 mRNA. Translation: CAA49628.1.
PIR	S30338.
3D structure databases
HSSP	HSSP built from PDB template 1DQA based on UniProtKB P04035.
ModBase	Search...
Family and domain databases
InterPro	IPR002202. HMG_CoA_Rdtase_cat. IPR004554. HMG_CoA_Rdtase_I_cat. IPR004816. HMG_CoA_Rdtase_I_metazoan. IPR000731. SSD_5TM. [Graphical view]
Gene3D	G3DSA:3.90.770.10. HMG-CoA_red. 1 hit.
PANTHER	PTHR10572. HMG-CoA_red. 1 hit.
Pfam	PF00368. HMG-CoA_red. 1 hit. [Graphical view]
PRINTS	PR00071. HMGCOARDTASE.
TIGRFAMs	TIGR00920. 2A060605. 1 hit. TIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITE	PS00066. HMG_COA_REDUCTASE_1. 1 hit. PS00318. HMG_COA_REDUCTASE_2. 1 hit. PS01192. HMG_COA_REDUCTASE_3. 1 hit. PS50065. HMG_COA_REDUCTASE_4. 1 hit. PS50156. SSD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMDH_BLAGE

Accession

Primary (citable) accession number: P54960

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 25, 2008
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)