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Protein

Aspartic protease Bla g 2

Gene
N/A
Organism
Blattella germanica (German cockroach) (Blatta germanica)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a digestive enzyme in the cockroach.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei55By similarity1
Metal bindingi178Zinc1
Metal bindingi186Zinc1
Active sitei239By similarity1
Metal bindingi326Zinc1
Metal bindingi330Zinc1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiA01.950.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartic protease Bla g 2 (EC:3.4.23.-)
Alternative name(s):
Allergen Bla g II
Allergen: Bla g 2
OrganismiBlattella germanica (German cockroach) (Blatta germanica)
Taxonomic identifieri6973 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraOrthopteroideaDictyopteraBlattodeaBlaberoideaEctobiidaeBlattellinaeBlattella

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Binds to IgE.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei141. Bla g 2.
3140. Bla g 2.0101.
4012. Bla g 2.0201.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
PropeptideiPRO_000002593520 – 24Removed in mature form5
ChainiPRO_000002593625 – 352Aspartic protease Bla g 2Add BLAST328

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi59 ↔ 151
Disulfide bondi68 ↔ 73
Disulfide bondi75 ↔ 136
Glycosylationi117N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi261 ↔ 272
Disulfide bondi276 ↔ 309
Glycosylationi295N-linked (GlcNAc...)2 Publications1
Glycosylationi340N-linked (GlcNAc...)2 Publications1

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

1352
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi31 – 35Combined sources5
Beta strandi39 – 45Combined sources7
Beta strandi48 – 55Combined sources8
Beta strandi61 – 64Combined sources4
Helixi71 – 74Combined sources4
Beta strandi87 – 89Combined sources3
Beta strandi94 – 98Combined sources5
Turni99 – 101Combined sources3
Beta strandi102 – 115Combined sources14
Beta strandi118 – 131Combined sources14
Helixi134 – 137Combined sources4
Beta strandi141 – 145Combined sources5
Beta strandi154 – 157Combined sources4
Helixi160 – 166Combined sources7
Beta strandi172 – 180Combined sources9
Beta strandi186 – 192Combined sources7
Helixi197 – 199Combined sources3
Beta strandi200 – 210Combined sources11
Beta strandi212 – 215Combined sources4
Beta strandi220 – 224Combined sources5
Beta strandi227 – 230Combined sources4
Beta strandi235 – 238Combined sources4
Beta strandi243 – 248Combined sources6
Helixi249 – 259Combined sources11
Beta strandi262 – 265Combined sources4
Beta strandi270 – 274Combined sources5
Helixi276 – 281Combined sources6
Beta strandi285 – 289Combined sources5
Beta strandi292 – 296Combined sources5
Helixi298 – 301Combined sources4
Beta strandi302 – 305Combined sources4
Beta strandi308 – 316Combined sources9
Beta strandi320 – 324Combined sources5
Helixi326 – 329Combined sources4
Beta strandi332 – 337Combined sources6
Turni338 – 341Combined sources4
Beta strandi342 – 348Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YG9X-ray1.30A25-348[»]
2NR6X-ray2.81A/B25-343[»]
3LIZX-ray1.80A25-352[»]
4RLDX-ray2.90A/B/C/D25-350[»]
ProteinModelPortaliP54958.
SMRiP54958.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP54958.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 346Peptidase A1PROSITE-ProRule annotationAdd BLAST308

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated
Contains 1 peptidase A1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P54958-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIGLKLVTVL FAVATITHAA ELQRVPLYKL VHVFINTQYA GITKIGNQNF
60 70 80 90 100
LTVFDSTSCN VVVASQECVG GACVCPNLQK YEKLKPKYIS DGNVQVKFFD
110 120 130 140 150
TGSAVGRGIE DSLTISNLTT SQQDIVLADE LSQEVCILSA DVVVGIAAPG
160 170 180 190 200
CPNALKGKTV LENFVEENLI APVFSIHHAR FQDGEHFGEI IFGGSDWKYV
210 220 230 240 250
DGEFTYVPLV GDDSWKFRLD GVKIGDTTVA PAGTQAIIDT SKAIIVGPKA
260 270 280 290 300
YVNPINEAIG CVVEKTTTRR ICKLDCSKIP SLPDVTFVIN GRNFNISSQY
310 320 330 340 350
YIQQNGNLCY SGFQPCGHSD HFFIGDFFVD HYYSEFNWEN KTMGFGRSVE

SV
Length:352
Mass (Da):38,558
Last modified:October 1, 1996 - v1
Checksum:iEA334CEB5D060A81
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6L → I in ABP35603 (Ref. 2) Curated1
Sequence conflicti87K → R in ABP35603 (Ref. 2) Curated1
Sequence conflicti116S → F in ABP35603 (Ref. 2) Curated1
Sequence conflicti142V → G in ABP35603 (Ref. 2) Curated1
Sequence conflicti342T → A in ABP35603 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28863 mRNA. Translation: AAA86744.1.
EF203068 mRNA. Translation: ABP35603.1.
PIRiA57164.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28863 mRNA. Translation: AAA86744.1.
EF203068 mRNA. Translation: ABP35603.1.
PIRiA57164.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YG9X-ray1.30A25-348[»]
2NR6X-ray2.81A/B25-343[»]
3LIZX-ray1.80A25-352[»]
4RLDX-ray2.90A/B/C/D25-350[»]
ProteinModelPortaliP54958.
SMRiP54958.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei141. Bla g 2.
3140. Bla g 2.0101.
4012. Bla g 2.0201.
MEROPSiA01.950.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP54958.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASP2_BLAGE
AccessioniPrimary (citable) accession number: P54958
Secondary accession number(s): B0L0M0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.