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P54958 (ASP2_BLAGE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aspartic protease Bla g 2
EC=3.4.23.-
Alternative name(s):
Allergen Bla g II
Allergen=Bla g 2
OrganismBlattella germanica (German cockroach) (Blatta germanica)
Taxonomic identifier6973 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Orthopteroidea › Dictyoptera › Blattodea › Blaberoidea › Ectobiidae › Blattellinae › Blattella

Protein attributes

Sequence length352 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as a digestive enzyme in the cockroach.

Subunit structure

Homodimer. Ref.3

Allergenic properties

Causes an allergic reaction in human. Binds to IgE.

Sequence similarities

Belongs to the peptidase A1 family.

Ontologies

Keywords
   DiseaseAllergen
   DomainSignal
   LigandMetal-binding
Zinc
   Molecular functionAspartyl protease
Hydrolase
Protease
   PTMDisulfide bond
Glycoprotein
Zymogen
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Biological processproteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionaspartic-type endopeptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Propeptide20 – 245Removed in mature form
PRO_0000025935
Chain25 – 352328Aspartic protease Bla g 2
PRO_0000025936

Sites

Active site551 By similarity
Active site2391 By similarity
Metal binding1781Zinc
Metal binding1861Zinc
Metal binding3261Zinc
Metal binding3301Zinc

Amino acid modifications

Glycosylation1171N-linked (GlcNAc...) Potential
Glycosylation2951N-linked (GlcNAc...) Ref.3 Ref.4
Glycosylation3401N-linked (GlcNAc...) Ref.3 Ref.4
Disulfide bond59 ↔ 151 Ref.3 Ref.4
Disulfide bond68 ↔ 73 Ref.3 Ref.4
Disulfide bond75 ↔ 136 Ref.3 Ref.4
Disulfide bond261 ↔ 272 Ref.3 Ref.4
Disulfide bond276 ↔ 309 Ref.3 Ref.4

Experimental info

Sequence conflict61L → I in ABP35603. Ref.2
Sequence conflict871K → R in ABP35603. Ref.2
Sequence conflict1161S → F in ABP35603. Ref.2
Sequence conflict1421V → G in ABP35603. Ref.2
Sequence conflict3421T → A in ABP35603. Ref.2

Secondary structure

1 ................................................................ 352
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P54958 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: EA334CEB5D060A81

FASTA35238,558
        10         20         30         40         50         60 
MIGLKLVTVL FAVATITHAA ELQRVPLYKL VHVFINTQYA GITKIGNQNF LTVFDSTSCN 

        70         80         90        100        110        120 
VVVASQECVG GACVCPNLQK YEKLKPKYIS DGNVQVKFFD TGSAVGRGIE DSLTISNLTT 

       130        140        150        160        170        180 
SQQDIVLADE LSQEVCILSA DVVVGIAAPG CPNALKGKTV LENFVEENLI APVFSIHHAR 

       190        200        210        220        230        240 
FQDGEHFGEI IFGGSDWKYV DGEFTYVPLV GDDSWKFRLD GVKIGDTTVA PAGTQAIIDT 

       250        260        270        280        290        300 
SKAIIVGPKA YVNPINEAIG CVVEKTTTRR ICKLDCSKIP SLPDVTFVIN GRNFNISSQY 

       310        320        330        340        350 
YIQQNGNLCY SGFQPCGHSD HFFIGDFFVD HYYSEFNWEN KTMGFGRSVE SV

« Hide

References

[1]"Molecular cloning of a major cockroach (Blattella germanica) allergen, Bla g 2. Sequence homology to the aspartic proteases."
Arruda L.K., Vailes L.D., Mann B.J., Shannon J., Fox J.W., Vedvick T.S., Hayden M.L., Chapman M.D.
J. Biol. Chem. 270:19563-19568(1995) [PubMed: 7642642] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]"cDNA sequence encoding a Bla g 2 isoform."
Lee H., Jeong K.Y., Yong T.-S.
Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Crystal structure of a dimerized cockroach allergen Bla g 2 complexed with a monoclonal antibody."
Li M., Gustchina A., Alexandratos J., Wlodawer A., Wunschmann S., Kepley C.L., Chapman M.D., Pomes A.
J. Biol. Chem. 283:22806-22814(2008) [PubMed: 18519566] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.81 ANGSTROMS) OF 25-350 IN COMPLEX WITH ANTIBODY, SUBUNIT, DISULFIDE BONDS, GLYCOSYLATION AT ASN-295 AND ASN-340.
[4]"Crystal structure of cockroach allergen Bla g 2, an unusual zinc binding aspartic protease with a novel mode of self-inhibition."
Gustchina A., Li M., Wunschmann S., Chapman M.D., Pomes A., Wlodawer A.
J. Mol. Biol. 348:433-444(2005) [PubMed: 15811379] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.3 ANGSTROMS) OF 29-348, DISULFIDE BONDS, ZINC BINDING, GLYCOSYLATION AT ASN-295 AND ASN-340.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U28863 mRNA. Translation: AAA86744.1.
EF203068 mRNA. Translation: ABP35603.1.
PIRA57164.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1YG9X-ray1.30A29-348[»]
2NR6X-ray2.81A/B25-350[»]
3LIZX-ray1.80A25-352[»]
ProteinModelPortalP54958.
SMRP54958. Positions 25-350.
ModBaseSearch...

Protein family/group databases

Allergome141. Bla g 2.
3140. Bla g 2.0101.
MEROPSA01.950.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR001461. Peptidase_A1.
IPR021109. Peptidase_aspartic.
IPR009007. Peptidase_aspartic_catalytic.
[Graphical view]
Gene3DG3DSA:2.40.70.10. Pept_Aspartc_cat. 2 hits.
PANTHERPTHR13683. Peptidase_A1. 1 hit.
PfamPF00026. Asp. 1 hit.
[Graphical view]
PRINTSPR00792. PEPSIN.
SUPFAMSSF50630. Pept_Aspartic. 1 hit.
PROSITEPS00141. ASP_PROTEASE. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASP2_BLAGE
AccessionPrimary (citable) accession number: P54958
Secondary accession number(s): B0L0M0
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 16, 2011
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

Allergens

Nomenclature of allergens and list of entries

Peptidase families

Classification of peptidase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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