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Protein

Endoglucanase A

Gene

celA

Organism
Clostridium longisporum
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes barley beta-glucan, lichenan, carboxymethylcellulose and xylan. It shows preferential activity against the larger cellooligosaccharides (cellohexaose and cellopentaose); cellotetraose is the smallest substrate degraded completely.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

pH dependencei

Optimum pH is 4.8.

Temperature dependencei

Optimum temperature is 43 degrees Celsius.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei185Proton donorBy similarity1
Active sitei309NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiCBM2 Carbohydrate-Binding Module Family 2
GH5 Glycoside Hydrolase Family 5

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase A (EC:3.2.1.4)
Alternative name(s):
Cellulase A
Endo-1,4-beta-glucanase A
Gene namesi
Name:celA
OrganismiClostridium longisporum
Taxonomic identifieri1523 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000000784926 – 517Endoglucanase AAdd BLAST492

Structurei

3D structure databases

ProteinModelPortaliP54937
SMRiP54937
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini416 – 517CBM2PROSITE-ProRule annotationAdd BLAST102

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni26 – ?CatalyticBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.40.290, 1 hit
InterProiView protein in InterPro
IPR001919 CBD2
IPR008965 CBM2/CBM3_carb-bd_dom_sf
IPR012291 CBM2_carb-bd_dom_sf
IPR001547 Glyco_hydro_5
IPR018087 Glyco_hydro_5_CS
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00553 CBM_2, 1 hit
PF00150 Cellulase, 1 hit
SMARTiView protein in SMART
SM00637 CBD_II, 1 hit
SUPFAMiSSF49384 SSF49384, 1 hit
SSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS51173 CBM2, 1 hit
PS00659 GLYCOSYL_HYDROL_F5, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P54937-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRSLLKTCS IIAGATIIFS SLSISRNPLE VQAASMRSAS EIVQEMGVGW
60 70 80 90 100
NLGNTLDAKI TNLSYNTSPI SFETGWGNPV TTKAMIDKIK NAGFKTIRIP
110 120 130 140 150
TTWGEHLDGN NKLNEEWVKR VKEVVDYCIA DDLYVILNTH HEGNWVIPTY
160 170 180 190 200
AKESSVTPKL KTLWTQISEA FKDYDDHLIF ETLNEPRLEG TPYEWTGGTS
210 220 230 240 250
ESRDVVNKYN AAALESIRKT GGNNLSRAVM MPTYAASGSS TTMNDFKVPD
260 270 280 290 300
DKNVIASVHA YSPYFFAMDT SSNSVNTWGS SYDKYSLDVE LDSYLNTFKS
310 320 330 340 350
KGVPVVIGEF GSINKNNTSS RAELAEYYVT AAQKRGIPCV WWDNNYAETN
360 370 380 390 400
KGETFGLLNR STLNWYFSDI KDALIRGYKN VHPEATEDDK PSTDVTNPDS
410 420 430 440 450
GNTKPDSGNT NPGTETTTPT DNEKISITSK INDWGGAYQA DFTLKNNTSS
460 470 480 490 500
DINNWSFKIK KNDIVFTNYW DVKITEENGY YVVTPQAWKT TILANSSIVI
510
SIQGTGKVIS NFEYKFD
Length:517
Mass (Da):57,660
Last modified:October 1, 1996 - v1
Checksum:iA1D1570302FFBA30
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L02868 Unassigned DNA Translation: AAC37035.1
PIRiI40798

Similar proteinsi

Entry informationi

Entry nameiGUNA_CLOLO
AccessioniPrimary (citable) accession number: P54937
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 25, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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