P54937 (GUNA_CLOLO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase A
EC=3.2.1.4

Alternative name(s):
Cellulase A
Endo-1,4-beta-glucanase A

Gene names

Name:

celA

Organism

Clostridium longisporum

Taxonomic identifier

1523 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	517 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes barley beta-glucan, lichenan, carboxymethylcellulose and xylan. It shows preferential activity against the larger cellooligosaccharides (cellohexaose and cellopentaose); cellotetraose is the smallest substrate degraded completely.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family. Contains 1 CBM2 (carbohydrate binding type-2) domain.
Biophysicochemical properties	pH dependence: Optimum pH is 4.8. Temperature dependence: Optimum temperature is 43 degrees Celsius.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC polysaccharide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 517	492	Endoglucanase A		PRO_0000007849
Regions
	Domain	416 – 517	102	CBM2
	Region	26 – ?		Catalytic By similarity
Sites
	Active site	185	1	Proton donor By similarity
	Active site	309	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	P54937 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: A1D1570302FFBA30	FASTA	517	57,660
	10 20 30 40 50 60 MKRSLLKTCS IIAGATIIFS SLSISRNPLE VQAASMRSAS EIVQEMGVGW NLGNTLDAKI 70 80 90 100 110 120 TNLSYNTSPI SFETGWGNPV TTKAMIDKIK NAGFKTIRIP TTWGEHLDGN NKLNEEWVKR 130 140 150 160 170 180 VKEVVDYCIA DDLYVILNTH HEGNWVIPTY AKESSVTPKL KTLWTQISEA FKDYDDHLIF 190 200 210 220 230 240 ETLNEPRLEG TPYEWTGGTS ESRDVVNKYN AAALESIRKT GGNNLSRAVM MPTYAASGSS 250 260 270 280 290 300 TTMNDFKVPD DKNVIASVHA YSPYFFAMDT SSNSVNTWGS SYDKYSLDVE LDSYLNTFKS 310 320 330 340 350 360 KGVPVVIGEF GSINKNNTSS RAELAEYYVT AAQKRGIPCV WWDNNYAETN KGETFGLLNR 370 380 390 400 410 420 STLNWYFSDI KDALIRGYKN VHPEATEDDK PSTDVTNPDS GNTKPDSGNT NPGTETTTPT 430 440 450 460 470 480 DNEKISITSK INDWGGAYQA DFTLKNNTSS DINNWSFKIK KNDIVFTNYW DVKITEENGY 490 500 510 YVVTPQAWKT TILANSSIVI SIQGTGKVIS NFEYKFD

References

[1]

"Cloning of an endo-(1-->4)-beta-glucanase gene, celA, from the rumen bacterium Clostridium sp. ('C. longisporum') and characterization of its product, CelA, in Escherichia coli." Mittendorf V., Thomson J.A. J. Gen. Microbiol. 139:3233-3242(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: ATCC 49440 / B6405.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L02868 Unassigned DNA. Translation: AAC37035.1.
PIR	I40798.
3D structure databases
ProteinModelPortal	P54937.
ModBase	Search...
Protein family/group databases
CAZy	CBM2. Carbohydrate-Binding Module Family 2. GH5. Glycoside Hydrolase Family 5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.60.40.290. 1 hit. 3.20.20.80. 1 hit.
InterPro	IPR008965. Carb-bd_dom. IPR012291. CBD_carb-bd_dom. IPR001919. Cellulose-bd_dom_fam2_bac. IPR001547. Glyco_hydro_5. IPR018087. Glyco_hydro_5_CS. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00553. CBM_2. 1 hit. PF00150. Cellulase. 1 hit. [Graphical view]
SMART	SM00637. CBD_II. 1 hit. [Graphical view]
SUPFAM	SSF49384. Cellul_bind. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS51173. CBM2. 1 hit. PS00659. GLYCOSYL_HYDROL_F5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUNA_CLOLO

Accession

Primary (citable) accession number: P54937

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program