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P54937 (GUNA_CLOLO) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglucanase A

EC=3.2.1.4
Alternative name(s):
Cellulase A
Endo-1,4-beta-glucanase A
Gene names
Name:celA
OrganismClostridium longisporum
Taxonomic identifier1523 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length517 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes barley beta-glucan, lichenan, carboxymethylcellulose and xylan. It shows preferential activity against the larger cellooligosaccharides (cellohexaose and cellopentaose); cellotetraose is the smallest substrate degraded completely.

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sequence similarities

Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Contains 1 CBM2 (carbohydrate binding type-2) domain.

Biophysicochemical properties

pH dependence:

Optimum pH is 4.8.

Temperature dependence:

Optimum temperature is 43 degrees Celsius.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cellulose degradation
Polysaccharide degradation
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processcellulose catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functioncellulase activity

Inferred from electronic annotation. Source: UniProtKB-EC

polysaccharide binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 517492Endoglucanase A
PRO_0000007849

Regions

Domain416 – 517102CBM2
Region26 – ?Catalytic By similarity

Sites

Active site1851Proton donor By similarity
Active site3091Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
P54937 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: A1D1570302FFBA30

FASTA51757,660
        10         20         30         40         50         60 
MKRSLLKTCS IIAGATIIFS SLSISRNPLE VQAASMRSAS EIVQEMGVGW NLGNTLDAKI 

        70         80         90        100        110        120 
TNLSYNTSPI SFETGWGNPV TTKAMIDKIK NAGFKTIRIP TTWGEHLDGN NKLNEEWVKR 

       130        140        150        160        170        180 
VKEVVDYCIA DDLYVILNTH HEGNWVIPTY AKESSVTPKL KTLWTQISEA FKDYDDHLIF 

       190        200        210        220        230        240 
ETLNEPRLEG TPYEWTGGTS ESRDVVNKYN AAALESIRKT GGNNLSRAVM MPTYAASGSS 

       250        260        270        280        290        300 
TTMNDFKVPD DKNVIASVHA YSPYFFAMDT SSNSVNTWGS SYDKYSLDVE LDSYLNTFKS 

       310        320        330        340        350        360 
KGVPVVIGEF GSINKNNTSS RAELAEYYVT AAQKRGIPCV WWDNNYAETN KGETFGLLNR 

       370        380        390        400        410        420 
STLNWYFSDI KDALIRGYKN VHPEATEDDK PSTDVTNPDS GNTKPDSGNT NPGTETTTPT 

       430        440        450        460        470        480 
DNEKISITSK INDWGGAYQA DFTLKNNTSS DINNWSFKIK KNDIVFTNYW DVKITEENGY 

       490        500        510 
YVVTPQAWKT TILANSSIVI SIQGTGKVIS NFEYKFD 

« Hide

References

[1]"Cloning of an endo-(1-->4)-beta-glucanase gene, celA, from the rumen bacterium Clostridium sp. ('C. longisporum') and characterization of its product, CelA, in Escherichia coli."
Mittendorf V., Thomson J.A.
J. Gen. Microbiol. 139:3233-3242(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
Strain: ATCC 49440 / B6405.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L02868 Unassigned DNA. Translation: AAC37035.1.
PIRI40798.

3D structure databases

ProteinModelPortalP54937.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM2. Carbohydrate-Binding Module Family 2.
GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.40.290. 1 hit.
3.20.20.80. 1 hit.
InterProIPR008965. Carb-bd_dom.
IPR012291. CBD_carb-bd_dom.
IPR001919. Cellulose-bd_dom_fam2_bac.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00553. CBM_2. 1 hit.
PF00150. Cellulase. 1 hit.
[Graphical view]
SMARTSM00637. CBD_II. 1 hit.
[Graphical view]
SUPFAMSSF49384. SSF49384. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEPS51173. CBM2. 1 hit.
PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUNA_CLOLO
AccessionPrimary (citable) accession number: P54937
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 16, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries