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Protein

Protein lin-2

Gene

lin-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a structural role in the induction of the vulva. May be required for the localization of signal transduction molecules (such as let-23 receptor) to either the basal membrane domain or the cell junctions.

GO - Molecular functioni

GO - Biological processi

  • gamma-aminobutyric acid receptor clustering Source: WormBase
  • oviposition Source: WormBase
  • positive regulation of vulval development Source: WormBase
  • post-embryonic development Source: WormBase
  • protein localization to basolateral plasma membrane Source: WormBase
Complete GO annotation...

Keywords - Ligandi

Calmodulin-binding

Enzyme and pathway databases

ReactomeiR-CEL-212676. Dopamine Neurotransmitter Release Cycle.
SignaLinkiP54936.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein lin-2
Alternative name(s):
Abnormal cell lineage protein 2
Gene namesi
Name:lin-2
ORF Names:F17E5.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiF17E5.1a; CE27131; WBGene00002991; lin-2.
F17E5.1b; CE27132; WBGene00002991; lin-2.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: WormBase
  • plasma membrane Source: GOC
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 961961Protein lin-2PRO_0000094572Add
BLAST

Proteomic databases

EPDiP54936.
PaxDbiP54936.
PRIDEiP54936.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
gmeb-3Q186243EBI-315019,EBI-319798
lin-10O175835EBI-315019,EBI-313389
lin-7Q9U2454EBI-315019,EBI-319872

GO - Molecular functioni

  • insulin-like growth factor receptor binding Source: WormBase

Protein-protein interaction databases

BioGridi46305. 18 interactions.
IntActiP54936. 60 interactions.
MINTiMINT-114176.
STRINGi6239.F17E5.1a.

Structurei

3D structure databases

ProteinModelPortaliP54936.
SMRiP54936. Positions 10-398, 543-960.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 281265Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini365 – 42258L27 1PROSITE-ProRule annotationAdd
BLAST
Domaini428 – 47952L27 2PROSITE-ProRule annotationAdd
BLAST
Domaini545 – 62076PDZPROSITE-ProRule annotationAdd
BLAST
Domaini633 – 71785SH3PROSITE-ProRule annotationAdd
BLAST
Domaini774 – 946173Guanylate kinase-likePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni310 – 32011Calmodulin-bindingAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi532 – 5365Poly-Glu
Compositional biasi738 – 7414Poly-Lys

Sequence similaritiesi

Belongs to the MAGUK family.Curated
Contains 1 guanylate kinase-like domain.PROSITE-ProRule annotation
Contains 2 L27 domains.PROSITE-ProRule annotation
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

eggNOGiKOG0033. Eukaryota.
KOG0609. Eukaryota.
COG0194. LUCA.
GeneTreeiENSGT00760000118866.
HOGENOMiHOG000233034.
InParanoidiP54936.
KOiK06103.
OMAiDGKCVVA.
OrthoDBiEOG79CXZ5.
PhylomeDBiP54936.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like_dom.
IPR020590. Guanylate_kinase_CS.
IPR011009. Kinase-like_dom.
IPR014775. L27_C.
IPR004172. L27_dom.
IPR027417. P-loop_NTPase.
IPR001478. PDZ.
IPR000719. Prot_kinase_dom.
IPR011511. SH3_2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF00625. Guanylate_kin. 1 hit.
PF02828. L27. 1 hit.
PF00595. PDZ. 1 hit.
PF00069. Pkinase. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]
SMARTiSM00072. GuKc. 1 hit.
SM00569. L27. 2 hits.
SM00228. PDZ. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
PS51022. L27. 2 hits.
PS50106. PDZ. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Lin-2a (identifier: P54936-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRELDPDESN LLLDEQLSIR DVIEQGPFSN VYRILHGPSN RKFLLRSINL
60 70 80 90 100
NLFRQHTGLG FEEIDEEIRI CQNLQHPYIC RLEKTINSVH YRHIIFENME
110 120 130 140 150
GNDICFEIVQ RASNGFVFSE YVVSHYTRQL LDALDYCHTR KIVHRDVRPH
160 170 180 190 200
NLVLASKDTS APLKLCGFGV AKDLSEIGGS MACGRVGVPQ FMAPEIVRKD
210 220 230 240 250
RVSCSSDIWS SGVVLFLLLA GRLPFSGSTS DIYERIMQTD VDVDGYMPNI
260 270 280 290 300
SESARNLVRR MLNADPSKRI SAKEALNHEW IRDKEHMASR KHMNDVIDQM
310 320 330 340 350
RRYNESRKLK SNVLSAVNSG RFDETTPRQD TPQTAFVDGS SPGGDCCHRG
360 370 380 390 400
ESSSNDAAEP PADKDLSGAY KVLGSLDAIN SLLDPNSYKP GSTTFQKIHD
410 420 430 440 450
DGSVRNLLRL YDKIKALPCE PVVTEVDTST LRKETLNQID GLLGPSPEAL
460 470 480 490 500
ELRQLLNSPH LASCVQALDV VVCEIRDPKN EASGSGDKEG NCVSSDPAPA
510 520 530 540 550
YLNGGVLPLG AQRAGTSFEH FNQSAVHTSY DEEEEELYDC MSRLRLVQFQ
560 570 580 590 600
KDTQEPMGIT LKVNEDGRCF VARIMHGGMI HRQATLHVGD EIREINGMSV
610 620 630 640 650
ANRSVESLQE MLRDARGQVT FKIIPSYRSA PPACEIFVRA QFDYEPSQDD
660 670 680 690 700
LIPCPQAGIP FKTGDILQVI SKDDHNWWQA RFVSSFPSIG NSSNAQRSNQ
710 720 730 740 750
QQVAGLIPSP ELQEWRTACL AMERSKNTCN THCMWFNKKK KYYTTKYLQK
760 770 780 790 800
HSALFDQLDL VTYEEVMRLS QYRRKTLVLL GAHGVGRRHI KNTLIHRHPN
810 820 830 840 850
RFAYPIPHTT RPPRKDEVDG KHYYFVTNEQ MMADIQNNEY LEYGTHEESM
860 870 880 890 900
YGTKLETIRN IHKSGKIAIL DVEPQALKVL RTAEYSPFVV FIAAPNLQGM
910 920 930 940 950
QDPDGSLEKL LNESDVLRQA FGHLFDFIIT NSDIDDTIAQ LERLVEKLPA
960
YPQWLPVTWV Y
Length:961
Mass (Da):108,812
Last modified:October 1, 1996 - v1
Checksum:i097D4B237AA0E788
GO
Isoform Lin-2b (identifier: P54936-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-341: Missing.
     342-409: PGGDCCHRGE...DDGSVRNLLR → MSSAATPPPM...VDITESSSTL

Show »
Length:620
Mass (Da):69,497
Checksum:iA349E346372A5B5C
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 341341Missing in isoform Lin-2b. 1 PublicationVSP_003154Add
BLAST
Alternative sequencei342 – 40968PGGDC…RNLLR → MSSAATPPPMEHTSSEDSTT APLQTPSSSSCLDVTVAVGT SVSYLKASPLVSSPTSLVVD ITESSSTL in isoform Lin-2b. 1 PublicationVSP_003155Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92564 mRNA. Translation: CAA63314.1.
X92565 mRNA. Translation: CAA63315.1.
Z50873 Genomic DNA. Translation: CAA90759.2.
Z50873 Genomic DNA. Translation: CAA90760.2.
PIRiT21072.
T21073.
RefSeqiNP_001024587.1. NM_001029416.2. [P54936-1]
NP_001024588.1. NM_001029417.2. [P54936-2]
UniGeneiCel.22759.

Genome annotation databases

EnsemblMetazoaiF17E5.1a; F17E5.1a; WBGene00002991. [P54936-1]
GeneIDi181400.
KEGGicel:CELE_F17E5.1.
UCSCiF17E5.1b. c. elegans. [P54936-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92564 mRNA. Translation: CAA63314.1.
X92565 mRNA. Translation: CAA63315.1.
Z50873 Genomic DNA. Translation: CAA90759.2.
Z50873 Genomic DNA. Translation: CAA90760.2.
PIRiT21072.
T21073.
RefSeqiNP_001024587.1. NM_001029416.2. [P54936-1]
NP_001024588.1. NM_001029417.2. [P54936-2]
UniGeneiCel.22759.

3D structure databases

ProteinModelPortaliP54936.
SMRiP54936. Positions 10-398, 543-960.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi46305. 18 interactions.
IntActiP54936. 60 interactions.
MINTiMINT-114176.
STRINGi6239.F17E5.1a.

Proteomic databases

EPDiP54936.
PaxDbiP54936.
PRIDEiP54936.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF17E5.1a; F17E5.1a; WBGene00002991. [P54936-1]
GeneIDi181400.
KEGGicel:CELE_F17E5.1.
UCSCiF17E5.1b. c. elegans. [P54936-1]

Organism-specific databases

CTDi181400.
WormBaseiF17E5.1a; CE27131; WBGene00002991; lin-2.
F17E5.1b; CE27132; WBGene00002991; lin-2.

Phylogenomic databases

eggNOGiKOG0033. Eukaryota.
KOG0609. Eukaryota.
COG0194. LUCA.
GeneTreeiENSGT00760000118866.
HOGENOMiHOG000233034.
InParanoidiP54936.
KOiK06103.
OMAiDGKCVVA.
OrthoDBiEOG79CXZ5.
PhylomeDBiP54936.

Enzyme and pathway databases

ReactomeiR-CEL-212676. Dopamine Neurotransmitter Release Cycle.
SignaLinkiP54936.

Miscellaneous databases

PROiP54936.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like_dom.
IPR020590. Guanylate_kinase_CS.
IPR011009. Kinase-like_dom.
IPR014775. L27_C.
IPR004172. L27_dom.
IPR027417. P-loop_NTPase.
IPR001478. PDZ.
IPR000719. Prot_kinase_dom.
IPR011511. SH3_2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF00625. Guanylate_kin. 1 hit.
PF02828. L27. 1 hit.
PF00595. PDZ. 1 hit.
PF00069. Pkinase. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]
SMARTiSM00072. GuKc. 1 hit.
SM00569. L27. 2 hits.
SM00228. PDZ. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
PS51022. L27. 2 hits.
PS50106. PDZ. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The C. elegans vulval induction gene lin-2 encodes a member of the MAGUK family of cell junction proteins."
    Hoskins R., Hajnal A.F., Harp S.A., Kim S.K.
    Development 122:97-111(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LIN-2A AND LIN-2B).
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiLIN2_CAEEL
AccessioniPrimary (citable) accession number: P54936
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 8, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.