P54924 (SQHC_BRAJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Squalene--hopene cyclase
EC=4.2.1.129
EC=5.4.99.17

Alternative name(s):
Squalene--hopanol cyclase

Gene names

Name:	shc
Ordered Locus Names:	blr3004

Organism

Bradyrhizobium japonicum (strain USDA 110) [Reference proteome] [HAMAP]

Taxonomic identifier

224911 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium › Bradyrhizobium japonicum

Protein attributes

Sequence length	660 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the cyclization of squalene into hopene.
Catalytic activity	Squalene = hop-22(29)-ene. Hopan-22-ol = squalene + H2O.
Pathway	Secondary metabolite biosynthesis; hopanoid biosynthesis.
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the terpene cyclase/mutase family. Contains 3 PFTB repeats.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Repeat
Molecular function	Isomerase Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	hopanoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	lyase activity Inferred from electronic annotation. Source: UniProtKB-KW squalene-hopene cyclase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 660	660	Squalene--hopene cyclase		PRO_0000072651
Regions
	Repeat	73 – 114	42	PFTB 1
	Repeat	419 – 460	42	PFTB 2
	Repeat	536 – 586	51	PFTB 3
Experimental info
	Sequence conflict	7 – 10	4	TARE → PP Ref.1
	Sequence conflict	347	1	V → G in CAA60250. Ref.1
	Sequence conflict	557	1	A → T in CAA60250. Ref.1
	Sequence conflict	641	1	A → G in CAA60250. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P54924 [UniParc]. Last modified February 28, 2003. Version 2. Checksum: FC3BBC9903959F66	FASTA	660	73,702
	10 20 30 40 50 60 MDSVNATARE AKESKISESE ILESSIASAT QGVLGFQQSD GHWVFELEAD CTIPAEYVLL 70 80 90 100 110 120 RHYLAEPVDT VLEAKIGNYL RRVQGAHGGW PLVHDGEFDM SASVKAYFAL KMIGDSIDAP 130 140 150 160 170 180 HMVRAREAIH ARGGAIHSNV FTRFMLAMFG IVTWRAVPVL PIEIMLLPFW SPFHINKISY 190 200 210 220 230 240 WARTTMVPLM VIAALKPRAK NPKGVGIDEL FLQDPRSIGM TAKAPHQSMA WFLLFRSLDA 250 260 270 280 290 300 ILRVIEPLFP KSLRKRAIDT ALAFSEERLN GEDGMGAIYP PMANLVMMYD ALGKDENYPP 310 320 330 340 350 360 RAVTRRGIDK LLVIGDDEAY CQPCVSPVWD TTLTAHALLE AGGDKAVPAA KHGLDWLIPK 370 380 390 400 410 420 QELEVKGDWA VKRPDVRPGG WAFQYNNAYY PDLDDTAVVV MSMDRMRREH GVTGYDSAID 430 440 450 460 470 480 RGREWIEGMQ SDDGGWAAFD VNNLEYYLNN IPFSDHGALL DPPTEDVTAR CVSMLAQLGE 490 500 510 520 530 540 TAKTSKHVAD GVAYLRKTQH PEGSWYGRWG MNFIYGTWSV LCALNMAGVR HDDPMIRKAA 550 560 570 580 590 600 DWLASIQNKD GGWGEDAVSY RLDYKGWEAA PSTASQTAWA LLALMAAGEV DHPAVARGVE 610 620 630 640 650 660 YLIATQNEKG LWDEQRYTAT GFPRVFYLRY HGYSKFFPLW ALARYRNLRN TNSRVVGVGM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Perzl M., Poralla K. Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X86552 Genomic DNA. Translation: CAA60250.1. BA000040 Genomic DNA. Translation: BAC48269.1.
RefSeq	NP_769644.1. NC_004463.1.
3D structure databases
ProteinModelPortal	P54924.
ModBase	Search...
Protein-protein interaction databases
STRING	224911.blr3004.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAC48269; BAC48269; BAC48269.
GeneID	1053386.
KEGG	bja:blr3004.
PATRIC	21189458. VBIBraJap65052_2987.
Phylogenomic databases
eggNOG	COG1657.
HOGENOM	HOG000220823.
KO	K06045.
OMA	NAYYPDV.
ProtClustDB	CLSK2393506.
Enzyme and pathway databases
BioCyc	BJAP224911:GJEJ-3028-MONOMER.
UniPathway	UPA00337.
Family and domain databases
InterPro	IPR006400. Hopene-cyclase. IPR001330. Prenyltrans. IPR018333. Squalene_cyclase. IPR002365. Terpene_synthase_CS. IPR008930. Terpenoid_cyclase/PrenylTrfase. [Graphical view]
Pfam	PF00432. Prenyltrans. 3 hits. [Graphical view]
SUPFAM	SSF48239. Terp_cyc_toroid. 2 hits.
TIGRFAMs	TIGR01507. hopene_cyclase. 1 hit. TIGR01787. squalene_cyclas. 1 hit.
PROSITE	PS01074. TERPENE_SYNTHASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SQHC_BRAJA

Accession

Primary (citable) accession number: P54924

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	February 28, 2003
Last modified:	May 1, 2013
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program