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Protein

Alpha-soluble NSF attachment protein

Gene

Napa

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus.By similarity

GO - Molecular functioni

  • SNARE binding Source: MGI
  • soluble NSF attachment protein activity Source: GO_Central
  • syntaxin binding Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-soluble NSF attachment protein
Short name:
SNAP-alpha
Alternative name(s):
N-ethylmaleimide-sensitive factor attachment protein alpha
Gene namesi
Name:Napa
Synonyms:Snap, Snapa
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi620855. Napa.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: Ensembl
  • synaptobrevin 2-SNAP-25-syntaxin-1a complex Source: MGI
  • terminal bouton Source: ParkinsonsUK-UCL
  • vacuolar membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 295295Alpha-soluble NSF attachment proteinPRO_0000219058Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei195 – 1951PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP54921.
PRIDEiP54921.

PTM databases

PhosphoSiteiP54921.

Expressioni

Gene expression databases

GenevisibleiP54921. RN.

Interactioni

Subunit structurei

Component of a SNARE-like complex that contains at least ZW10, USE1L, RINT1, STX18 and NAPA/SNAP-alpha (By similarity). Interacts with PRKCABP, and disrupts the interaction between GRIA2 and PRKCABP, leading to the internalization of GRIA2. Found in a complex with VAMP8. Interacts with VTI1A (By similarity). Interacts with STX12.By similarity3 Publications

Protein-protein interaction databases

IntActiP54921. 1 interaction.
MINTiMINT-4542616.
STRINGi10116.ENSRNOP00000002044.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J96electron microscopy7.60G/H/I/J1-295[»]
3J97electron microscopy7.80G/H/I/J1-295[»]
3J98electron microscopy8.40G/H/I/J1-295[»]
3J99electron microscopy8.20G/H/I/J1-295[»]
ProteinModelPortaliP54921.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SNAP family.Curated

Phylogenomic databases

eggNOGiNOG245914.
GeneTreeiENSGT00390000005826.
HOGENOMiHOG000165015.
HOVERGENiHBG001325.
InParanoidiP54921.
KOiK15296.
OMAiMQSKHDA.
OrthoDBiEOG7FNC80.
PhylomeDBiP54921.
TreeFamiTF316547.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR000744. NSF_attach.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PANTHERiPTHR13768. PTHR13768. 1 hit.
PRINTSiPR00448. NSFATTACHMNT.

Sequencei

Sequence statusi: Complete.

P54921-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTSGKQAEA MALLAEAERK VKNSQSFFSG LFGGSSKIEE ACEIYARAAN
60 70 80 90 100
MFKMAKNWSA AGNAFCQAAQ LHLQLQSKHD AATCFVDAGN AFKKADPQEA
110 120 130 140 150
INCLMRAIEI YTDMGRFTIA AKHHISIAEI YETELVDVEK AIAHYEQSAD
160 170 180 190 200
YYKGEESNSS ANKCLLKVAG YAAQLEQYQK AIDIYEQVGT SAMDSPLLKY
210 220 230 240 250
SAKDYFFKAA LCHFCIDMLN AKLAVQKYEE LFPAFSDSRE CKLMKKLLEA
260 270 280 290
HEEQNVDSYT ESVKEYDSIS RLDQWLTTML LRIKKTIQGD EEDLR
Length:295
Mass (Da):33,193
Last modified:July 15, 1999 - v2
Checksum:i662356859DF0BA3F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89968 mRNA. Translation: CAA62005.1.
BC063156 mRNA. Translation: AAH63156.1.
PIRiS58285.
RefSeqiNP_542152.1. NM_080585.1.
UniGeneiRn.31745.

Genome annotation databases

EnsembliENSRNOT00000002044; ENSRNOP00000002044; ENSRNOG00000001494.
GeneIDi140673.
KEGGirno:140673.
UCSCiRGD:620855. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89968 mRNA. Translation: CAA62005.1.
BC063156 mRNA. Translation: AAH63156.1.
PIRiS58285.
RefSeqiNP_542152.1. NM_080585.1.
UniGeneiRn.31745.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J96electron microscopy7.60G/H/I/J1-295[»]
3J97electron microscopy7.80G/H/I/J1-295[»]
3J98electron microscopy8.40G/H/I/J1-295[»]
3J99electron microscopy8.20G/H/I/J1-295[»]
ProteinModelPortaliP54921.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP54921. 1 interaction.
MINTiMINT-4542616.
STRINGi10116.ENSRNOP00000002044.

PTM databases

PhosphoSiteiP54921.

Proteomic databases

PaxDbiP54921.
PRIDEiP54921.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000002044; ENSRNOP00000002044; ENSRNOG00000001494.
GeneIDi140673.
KEGGirno:140673.
UCSCiRGD:620855. rat.

Organism-specific databases

CTDi8775.
RGDi620855. Napa.

Phylogenomic databases

eggNOGiNOG245914.
GeneTreeiENSGT00390000005826.
HOGENOMiHOG000165015.
HOVERGENiHBG001325.
InParanoidiP54921.
KOiK15296.
OMAiMQSKHDA.
OrthoDBiEOG7FNC80.
PhylomeDBiP54921.
TreeFamiTF316547.

Miscellaneous databases

NextBioi620663.
PROiP54921.

Gene expression databases

GenevisibleiP54921. RN.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR000744. NSF_attach.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PANTHERiPTHR13768. PTHR13768. 1 hit.
PRINTSiPR00448. NSFATTACHMNT.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Mitchell J.R.D., Mitchell M., Holman D., Oldfield S.
    Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: White adipose tissue.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pituitary.
  3. "Syntaxin 12, a member of the syntaxin family localized to the endosome."
    Tang B.L., Tan A.E., Lim L.K., Lee S.S., Low D.Y., Hong W.
    J. Biol. Chem. 273:6944-6950(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH STX12.
  4. "Endobrevin, a novel synaptobrevin/VAMP-like protein preferentially associated with the early endosome."
    Wong S.H., Zhang T., Xu Y., Subramaniam V.N., Griffiths G., Hong W.
    Mol. Biol. Cell 9:1549-1563(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A COMPLEX WITH VAMP8.
  5. "NSF ATPase and alpha-/beta-SNAPs disassemble the AMPA receptor-PICK1 complex."
    Hanley J.G., Khatri L., Hanson P.I., Ziff E.B.
    Neuron 34:53-67(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PRKCABP.

Entry informationi

Entry nameiSNAA_RAT
AccessioniPrimary (citable) accession number: P54921
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 15, 1999
Last modified: June 24, 2015
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.