P54920 (SNAA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-soluble NSF attachment protein
Short name=SNAP-alpha

Alternative name(s):
N-ethylmaleimide-sensitive factor attachment protein alpha

Gene names

Name:	NAPA
Synonyms:	SNAPA

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus.
Subunit structure	Interacts with PRKCABP, and disrupts the interaction between GRIA2 and PRKCABP, leading to the internalization of GRIA2. Found in a complex with VAMP8 By similarity. Component of a SNARE-like complex that contains at least ZW10, USE1L, RINT1, STX18 and NAPA/SNAP-alpha. Interacts with VTI1A. Interacts with STX12 By similarity. Ref.7
Subcellular location	Membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the SNAP family.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Membrane
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	SNARE complex disassembly Inferred from electronic annotation. Source: Compara apical protein localization Inferred from electronic annotation. Source: Compara brain development Inferred from electronic annotation. Source: Compara cellular membrane fusion Traceable author statement PubMed 8455721. Source: ProtInc intra-Golgi vesicle-mediated transport Traceable author statement PubMed 8455721. Source: ProtInc intracellular protein transport Inferred from electronic annotation. Source: Compara neuron differentiation Inferred from electronic annotation. Source: Compara post-Golgi vesicle-mediated transport Traceable author statement. Source: Reactome regulation of synaptic vesicle priming Inferred from electronic annotation. Source: Compara synaptic transmission, glutamatergic Inferred from electronic annotation. Source: Compara
Cellular_component	cytosol Traceable author statement. Source: Reactome synaptobrevin 2-SNAP-25-syntaxin-1a complex Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SNAP23	O00161	2	EBI-749652,EBI-745000
STX4	Q12846	2	EBI-749652,EBI-744942

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 295	295	Alpha-soluble NSF attachment protein		PRO_0000219056
Experimental info
	Sequence conflict	195	1	S → T in AAC80170. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P54920 [UniParc]. Last modified October 17, 2006. Version 3. Checksum: 7704A619A441E609	FASTA	295	33,233
	10 20 30 40 50 60 MDNSGKEAEA MALLAEAERK VKNSQSFFSG LFGGSSKIEE ACEIYARAAN MFKMAKNWSA 70 80 90 100 110 120 AGNAFCQAAQ LHLQLQSKHD AATCFVDAGN AFKKADPQEA INCLMRAIEI YTDMGRFTIA 130 140 150 160 170 180 AKHHISIAEI YETELVDIEK AIAHYEQSAD YYKGEESNSS ANKCLLKVAG YAALLEQYQK 190 200 210 220 230 240 AIDIYEQVGT NAMDSPLLKY SAKDYFFKAA LCHFCIDMLN AKLAVQKYEE LFPAFSDSRE 250 260 270 280 290 CKLMKKLLEA HEEQNVDSYT ESVKEYDSIS RLDQWLTTML LRIKKTIQGD EEDLR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Regulated secretion in platelets: identification of elements of the platelet exocytosis machinery." Lemons P.P., Chen D., Bernstein A.M., Bennett M.K., Whiteheart S.W. Blood 90:1490-1500(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Platelet.
[2]	Chen D., Shao H.P., Whiteheart S.W. Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 91; 133 AND 289.
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain, Lung and Placenta.
[7]	"Implication of ZW10 in membrane trafficking between the endoplasmic reticulum and Golgi." Hirose H., Arasaki K., Dohmae N., Takio K., Hatsuzawa K., Nagahama M., Tani K., Yamamoto A., Tohyama M., Tagaya M. EMBO J. 23:1267-1278(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A COMPLEX WITH RINT1; USE1L; NAPA AND ZW10.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U39412 mRNA. Translation: AAC80170.1. BT019568 mRNA. Translation: AAV38375.1. AK292244 mRNA. Translation: BAF84933.1. CH471126 Genomic DNA. Translation: EAW57491.1. BC001165 mRNA. Translation: AAH01165.1. BC007432 mRNA. Translation: AAH07432.1. BC028234 mRNA. Translation: AAH28234.1. BC091511 mRNA. Translation: AAH91511.1.
IPI	IPI00009253.
PIR	G02238.
RefSeq	NP_003818.2. NM_003827.3.
UniGene	Hs.126938. Hs.731414.
3D structure databases
ProteinModelPortal	P54920.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-393N.
IntAct	P54920. 7 interactions.
MINT	MINT-3020680.
STRING	9606.ENSP00000263354.
PTM databases
PhosphoSite	P54920.
Polymorphism databases
DMDM	116242794.
2D gel databases
OGP	P54920.
Proteomic databases
PaxDb	P54920.
PeptideAtlas	P54920.
PRIDE	P54920.
Protocols and materials databases
DNASU	8775.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000263354; ENSP00000263354; ENSG00000105402.
GeneID	8775.
KEGG	hsa:8775.
UCSC	uc002pha.2. human.
Organism-specific databases
CTD	8775.
GeneCards	GC19M047990.
H-InvDB	HIX0137469.
HGNC	HGNC:7641. NAPA.
HPA	CAB010100.
MIM	603215. gene.
neXtProt	NX_P54920.
PharmGKB	PA31443.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG245914.
HOGENOM	HOG000165015.
HOVERGEN	HBG001325.
InParanoid	P54920.
KO	K15296.
OMA	INCLNRA.
OrthoDB	EOG428227.
PhylomeDB	P54920.
Enzyme and pathway databases
Reactome	REACT_11123. Membrane Trafficking.
Gene expression databases
Bgee	P54920.
CleanEx	HS_NAPA.
Genevestigator	P54920.
GermOnline	ENSG00000105402. Homo sapiens.
Family and domain databases
Gene3D	1.25.40.10. 1 hit.
InterPro	IPR000744. NSF_attach. IPR011990. TPR-like_helical. [Graphical view]
PANTHER	PTHR13768. PTHR13768. 1 hit.
PRINTS	PR00448. NSFATTACHMNT.
ProtoNet	Search...
Other
GenomeRNAi	8775.
NextBio	32904.
PMAP-CutDB	P54920.
SOURCE	Search...

Entry information

Entry name

SNAA_HUMAN

Accession

Primary (citable) accession number: P54920
Secondary accession number(s): A8K879, Q96IK3, Q9BVJ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 17, 2006
Last modified:	May 1, 2013
This is version 120 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.