Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alpha-soluble NSF attachment protein

Gene

NAPA

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

ReactomeiREACT_19400. Golgi Associated Vesicle Biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-soluble NSF attachment protein
Short name:
SNAP-alpha
Alternative name(s):
N-ethylmaleimide-sensitive factor attachment protein alpha
Gene namesi
Name:NAPA
Synonyms:SNAPA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:7641. NAPA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
  • membrane Source: UniProtKB
  • myelin sheath Source: Ensembl
  • SNARE complex Source: GO_Central
  • synaptobrevin 2-SNAP-25-syntaxin-1a complex Source: Ensembl
  • terminal bouton Source: Ensembl
  • vacuolar membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA31443.

Polymorphism and mutation databases

BioMutaiNAPA.
DMDMi116242794.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 295295Alpha-soluble NSF attachment proteinPRO_0000219056Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication
Modified residuei195 – 1951Phosphoserine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP54920.
PaxDbiP54920.
PeptideAtlasiP54920.
PRIDEiP54920.

2D gel databases

OGPiP54920.

PTM databases

PhosphoSiteiP54920.

Miscellaneous databases

PMAP-CutDBP54920.

Expressioni

Gene expression databases

BgeeiP54920.
CleanExiHS_NAPA.
ExpressionAtlasiP54920. baseline and differential.
GenevisibleiP54920. HS.

Organism-specific databases

HPAiCAB010100.
HPA046149.
HPA050196.

Interactioni

Subunit structurei

Interacts with PRKCABP, and disrupts the interaction between GRIA2 and PRKCABP, leading to the internalization of GRIA2. Found in a complex with VAMP8 (By similarity). Component of a SNARE-like complex that contains at least ZW10, USE1L, RINT1, STX18 and NAPA/SNAP-alpha. Interacts with VTI1A. Interacts with STX12 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
NSFP464592EBI-749652,EBI-712251
SNAP23O001612EBI-749652,EBI-745000
STX4Q128462EBI-749652,EBI-744942

Protein-protein interaction databases

BioGridi114305. 34 interactions.
DIPiDIP-393N.
IntActiP54920. 8 interactions.
MINTiMINT-3020680.
STRINGi9606.ENSP00000263354.

Structurei

3D structure databases

ProteinModelPortaliP54920.
SMRiP54920. Positions 19-293.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SNAP family.Curated

Phylogenomic databases

eggNOGiNOG245914.
GeneTreeiENSGT00390000005826.
HOGENOMiHOG000165015.
HOVERGENiHBG001325.
InParanoidiP54920.
KOiK15296.
OMAiINCLNRA.
OrthoDBiEOG7FNC80.
PhylomeDBiP54920.
TreeFamiTF316547.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR000744. NSF_attach.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PANTHERiPTHR13768. PTHR13768. 1 hit.
PRINTSiPR00448. NSFATTACHMNT.

Sequencei

Sequence statusi: Complete.

P54920-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNSGKEAEA MALLAEAERK VKNSQSFFSG LFGGSSKIEE ACEIYARAAN
60 70 80 90 100
MFKMAKNWSA AGNAFCQAAQ LHLQLQSKHD AATCFVDAGN AFKKADPQEA
110 120 130 140 150
INCLMRAIEI YTDMGRFTIA AKHHISIAEI YETELVDIEK AIAHYEQSAD
160 170 180 190 200
YYKGEESNSS ANKCLLKVAG YAALLEQYQK AIDIYEQVGT NAMDSPLLKY
210 220 230 240 250
SAKDYFFKAA LCHFCIDMLN AKLAVQKYEE LFPAFSDSRE CKLMKKLLEA
260 270 280 290
HEEQNVDSYT ESVKEYDSIS RLDQWLTTML LRIKKTIQGD EEDLR
Length:295
Mass (Da):33,233
Last modified:October 17, 2006 - v3
Checksum:i7704A619A441E609
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti195 – 1951S → T in AAC80170 (PubMed:9269766).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39412 mRNA. Translation: AAC80170.1.
BT019568 mRNA. Translation: AAV38375.1.
AK292244 mRNA. Translation: BAF84933.1.
CH471126 Genomic DNA. Translation: EAW57491.1.
BC001165 mRNA. Translation: AAH01165.1.
BC007432 mRNA. Translation: AAH07432.1.
BC028234 mRNA. Translation: AAH28234.1.
BC091511 mRNA. Translation: AAH91511.1.
CCDSiCCDS12702.1.
PIRiG02238.
RefSeqiNP_003818.2. NM_003827.3.
UniGeneiHs.730799.
Hs.744881.

Genome annotation databases

EnsembliENST00000263354; ENSP00000263354; ENSG00000105402.
GeneIDi8775.
KEGGihsa:8775.
UCSCiuc002pha.2. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39412 mRNA. Translation: AAC80170.1.
BT019568 mRNA. Translation: AAV38375.1.
AK292244 mRNA. Translation: BAF84933.1.
CH471126 Genomic DNA. Translation: EAW57491.1.
BC001165 mRNA. Translation: AAH01165.1.
BC007432 mRNA. Translation: AAH07432.1.
BC028234 mRNA. Translation: AAH28234.1.
BC091511 mRNA. Translation: AAH91511.1.
CCDSiCCDS12702.1.
PIRiG02238.
RefSeqiNP_003818.2. NM_003827.3.
UniGeneiHs.730799.
Hs.744881.

3D structure databases

ProteinModelPortaliP54920.
SMRiP54920. Positions 19-293.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114305. 34 interactions.
DIPiDIP-393N.
IntActiP54920. 8 interactions.
MINTiMINT-3020680.
STRINGi9606.ENSP00000263354.

PTM databases

PhosphoSiteiP54920.

Polymorphism and mutation databases

BioMutaiNAPA.
DMDMi116242794.

2D gel databases

OGPiP54920.

Proteomic databases

MaxQBiP54920.
PaxDbiP54920.
PeptideAtlasiP54920.
PRIDEiP54920.

Protocols and materials databases

DNASUi8775.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000263354; ENSP00000263354; ENSG00000105402.
GeneIDi8775.
KEGGihsa:8775.
UCSCiuc002pha.2. human.

Organism-specific databases

CTDi8775.
GeneCardsiGC19M047990.
H-InvDBHIX0137469.
HGNCiHGNC:7641. NAPA.
HPAiCAB010100.
HPA046149.
HPA050196.
MIMi603215. gene.
neXtProtiNX_P54920.
PharmGKBiPA31443.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG245914.
GeneTreeiENSGT00390000005826.
HOGENOMiHOG000165015.
HOVERGENiHBG001325.
InParanoidiP54920.
KOiK15296.
OMAiINCLNRA.
OrthoDBiEOG7FNC80.
PhylomeDBiP54920.
TreeFamiTF316547.

Enzyme and pathway databases

ReactomeiREACT_19400. Golgi Associated Vesicle Biogenesis.

Miscellaneous databases

ChiTaRSiNAPA. human.
GeneWikiiNAPA_(gene).
GenomeRNAii8775.
NextBioi32904.
PMAP-CutDBP54920.
PROiP54920.
SOURCEiSearch...

Gene expression databases

BgeeiP54920.
CleanExiHS_NAPA.
ExpressionAtlasiP54920. baseline and differential.
GenevisibleiP54920. HS.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR000744. NSF_attach.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PANTHERiPTHR13768. PTHR13768. 1 hit.
PRINTSiPR00448. NSFATTACHMNT.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Regulated secretion in platelets: identification of elements of the platelet exocytosis machinery."
    Lemons P.P., Chen D., Bernstein A.M., Bennett M.K., Whiteheart S.W.
    Blood 90:1490-1500(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Platelet.
  2. Chen D., Shao H.P., Whiteheart S.W.
    Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 91; 133 AND 289.
  3. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain, Lung and Placenta.
  7. "Implication of ZW10 in membrane trafficking between the endoplasmic reticulum and Golgi."
    Hirose H., Arasaki K., Dohmae N., Takio K., Hatsuzawa K., Nagahama M., Tani K., Yamamoto A., Tohyama M., Tagaya M.
    EMBO J. 23:1267-1278(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A COMPLEX WITH RINT1; USE1L; NAPA AND ZW10.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-195, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiSNAA_HUMAN
AccessioniPrimary (citable) accession number: P54920
Secondary accession number(s): A8K879, Q96IK3, Q9BVJ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 17, 2006
Last modified: July 22, 2015
This is version 144 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.