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Protein

Alpha-soluble NSF attachment protein

Gene

NAPA

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus (Probable). Together with GNA12 promotes CDH5 localization to plasma membrane (PubMed:15980433).Curated1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-HSA-432722. Golgi Associated Vesicle Biogenesis.
R-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-HSA-6811438. Intra-Golgi traffic.
R-HSA-6811440. Retrograde transport at the Trans-Golgi-Network.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-soluble NSF attachment protein
Short name:
SNAP-alpha
Alternative name(s):
N-ethylmaleimide-sensitive factor attachment protein alpha
Gene namesi
Name:NAPA
Synonyms:SNAPA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000105402.7.
HGNCiHGNC:7641. NAPA.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi122K → A: Does not affect interaction with GNA12. 1 Publication1
Mutagenesisi140K → A: Increases interaction with GNA12. 1 Publication1

Organism-specific databases

DisGeNETi8775.
OpenTargetsiENSG00000105402.
PharmGKBiPA31443.

Polymorphism and mutation databases

BioMutaiNAPA.
DMDMi116242794.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002190561 – 295Alpha-soluble NSF attachment proteinAdd BLAST295

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei26PhosphoserineBy similarity1
Modified residuei29PhosphoserineCombined sources1
Modified residuei195PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP54920.
MaxQBiP54920.
PaxDbiP54920.
PeptideAtlasiP54920.
PRIDEiP54920.
TopDownProteomicsiP54920.

2D gel databases

OGPiP54920.

PTM databases

iPTMnetiP54920.
PhosphoSitePlusiP54920.
SwissPalmiP54920.

Miscellaneous databases

PMAP-CutDBiP54920.

Expressioni

Gene expression databases

BgeeiENSG00000105402.
CleanExiHS_NAPA.
ExpressionAtlasiP54920. baseline and differential.
GenevisibleiP54920. HS.

Organism-specific databases

HPAiCAB010100.
HPA046149.
HPA050196.

Interactioni

Subunit structurei

Interacts with PRKCABP, and disrupts the interaction between GRIA2 and PRKCABP, leading to the internalization of GRIA2 (By similarity). Found in a complex with VAMP8 (By similarity). Component of a SNARE-like complex that contains at least ZW10, USE1L, RINT1, STX18 and NAPA/SNAP-alpha (PubMed:15029241). Interacts with VTI1A (By similarity). Interacts with STX12 (By similarity). Interacts with GNA12 (via N-terminus); the interaction promotes CDH5 localization to plasma membrane (PubMed:15980433).By similarity2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi114305. 53 interactors.
IntActiP54920. 11 interactors.
MINTiMINT-3020680.
STRINGi9606.ENSP00000263354.

Structurei

3D structure databases

ProteinModelPortaliP54920.
SMRiP54920.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SNAP family.Curated

Phylogenomic databases

eggNOGiKOG1586. Eukaryota.
ENOG410XPQ6. LUCA.
GeneTreeiENSGT00390000005826.
HOGENOMiHOG000165015.
HOVERGENiHBG001325.
InParanoidiP54920.
KOiK15296.
OMAiMADNEQK.
OrthoDBiEOG091G0KSY.
PhylomeDBiP54920.
TreeFamiTF316547.

Family and domain databases

CDDicd15832. SNAP. 1 hit.
Gene3Di1.25.40.10. 1 hit.
InterProiView protein in InterPro
IPR000744. NSF_attach.
IPR011990. TPR-like_helical_dom_sf.
PANTHERiPTHR13768. PTHR13768. 1 hit.
PRINTSiPR00448. NSFATTACHMNT.
SUPFAMiSSF48452. SSF48452. 1 hit.

Sequencei

Sequence statusi: Complete.

P54920-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNSGKEAEA MALLAEAERK VKNSQSFFSG LFGGSSKIEE ACEIYARAAN
60 70 80 90 100
MFKMAKNWSA AGNAFCQAAQ LHLQLQSKHD AATCFVDAGN AFKKADPQEA
110 120 130 140 150
INCLMRAIEI YTDMGRFTIA AKHHISIAEI YETELVDIEK AIAHYEQSAD
160 170 180 190 200
YYKGEESNSS ANKCLLKVAG YAALLEQYQK AIDIYEQVGT NAMDSPLLKY
210 220 230 240 250
SAKDYFFKAA LCHFCIDMLN AKLAVQKYEE LFPAFSDSRE CKLMKKLLEA
260 270 280 290
HEEQNVDSYT ESVKEYDSIS RLDQWLTTML LRIKKTIQGD EEDLR
Length:295
Mass (Da):33,233
Last modified:October 17, 2006 - v3
Checksum:i7704A619A441E609
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti195S → T in AAC80170 (PubMed:9269766).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39412 mRNA. Translation: AAC80170.1.
BT019568 mRNA. Translation: AAV38375.1.
AK292244 mRNA. Translation: BAF84933.1.
CH471126 Genomic DNA. Translation: EAW57491.1.
BC001165 mRNA. Translation: AAH01165.1.
BC007432 mRNA. Translation: AAH07432.1.
BC028234 mRNA. Translation: AAH28234.1.
BC091511 mRNA. Translation: AAH91511.1.
CCDSiCCDS12702.1.
PIRiG02238.
RefSeqiNP_003818.2. NM_003827.3.
UniGeneiHs.126938.
Hs.730799.
Hs.744881.

Genome annotation databases

EnsembliENST00000263354; ENSP00000263354; ENSG00000105402.
GeneIDi8775.
KEGGihsa:8775.
UCSCiuc002pha.3. human.

Similar proteinsi

Entry informationi

Entry nameiSNAA_HUMAN
AccessioniPrimary (citable) accession number: P54920
Secondary accession number(s): A8K879, Q96IK3, Q9BVJ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 17, 2006
Last modified: November 22, 2017
This is version 166 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families