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P54919

- HEM2_STRCO

UniProt

P54919 - HEM2_STRCO

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Protein
Delta-aminolevulinic acid dehydratase
Gene
hemB, SCO3311, SCE68.09c
Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen By similarity.

Catalytic activityi

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei203 – 2031Schiff-base intermediate with substrate By similarity
Binding sitei213 – 2131Substrate 1 By similarity
Binding sitei224 – 2241Substrate 1 By similarity
Metal bindingi240 – 2401Magnesium By similarity
Active sitei255 – 2551Schiff-base intermediate with substrate By similarity
Binding sitei281 – 2811Substrate 2 By similarity
Binding sitei320 – 3201Substrate 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. porphobilinogen synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00251; UER00318.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
Short name:
ALAD
Short name:
ALADH
Alternative name(s):
Porphobilinogen synthase
Gene namesi
Name:hemB
Ordered Locus Names:SCO3311
ORF Names:SCE68.09c
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
ProteomesiUP000001973: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 330330Delta-aminolevulinic acid dehydratase
PRO_0000140519Add
BLAST

Proteomic databases

PRIDEiP54919.

Interactioni

Subunit structurei

Homooctamer By similarity.

Protein-protein interaction databases

STRINGi100226.SCO3311.

Structurei

3D structure databases

ProteinModelPortaliP54919.

Family & Domainsi

Sequence similaritiesi

Belongs to the ALADH family.

Phylogenomic databases

eggNOGiCOG0113.
HOGENOMiHOG000020323.
KOiK01698.
OMAiDMILTYF.
OrthoDBiEOG6VXFCB.
PhylomeDBiP54919.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view]
PANTHERiPTHR11458. PTHR11458. 1 hit.
PfamiPF00490. ALAD. 1 hit.
[Graphical view]
PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
PRINTSiPR00144. DALDHYDRTASE.
SMARTiSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54919-1 [UniParc]FASTAAdd to Basket

« Hide

MTTYGSFPGA RPRRLRTTPV MRRMVAETRL HPADFILPAF VREGVSEPVP    50
IAAMPGVVQH TRDTLKKAAA EAVEAGVSGI MLFGVPEDGK KDAAGTAGTD 100
PDGILQVALR DVRAEVGDEL LVMSDLCLDE FTDHGHCGVL DGQGRVDNDA 150
TLERYAEMAQ VQADAGAHVV GPSGMMDGQI GVIRDALDQI GREDVAILAY 200
TAKYASAFYG PFREAVGSSL KGDRKTYQQD SANARESLRE LALDLEEGAD 250
MVMVKPAGPY LDILAKVAEA SDVPVAAYQI SGEYSMIEAA AEKGWIDRDR 300
AILESLTGIK RAGARNILTY WATEVARTLR 330
Length:330
Mass (Da):35,453
Last modified:December 1, 2000 - v2
Checksum:iD2AF1F8B971F30F5
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti100 – 1001D → V in AAA61398. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U19249 Genomic DNA. Translation: AAA61398.1.
AL939116 Genomic DNA. Translation: CAB45345.1.
PIRiT36259.
RefSeqiNP_627521.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAB45345; CAB45345; CAB45345.
GeneIDi1098745.
KEGGisco:SCO3311.
PATRICi23736366. VBIStrCoe124346_3371.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U19249 Genomic DNA. Translation: AAA61398.1 .
AL939116 Genomic DNA. Translation: CAB45345.1 .
PIRi T36259.
RefSeqi NP_627521.1. NC_003888.3.

3D structure databases

ProteinModelPortali P54919.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 100226.SCO3311.

Proteomic databases

PRIDEi P54919.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB45345 ; CAB45345 ; CAB45345 .
GeneIDi 1098745.
KEGGi sco:SCO3311.
PATRICi 23736366. VBIStrCoe124346_3371.

Phylogenomic databases

eggNOGi COG0113.
HOGENOMi HOG000020323.
KOi K01698.
OMAi DMILTYF.
OrthoDBi EOG6VXFCB.
PhylomeDBi P54919.

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00318 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view ]
PANTHERi PTHR11458. PTHR11458. 1 hit.
Pfami PF00490. ALAD. 1 hit.
[Graphical view ]
PIRSFi PIRSF001415. Porphbilin_synth. 1 hit.
PRINTSi PR00144. DALDHYDRTASE.
SMARTi SM01004. ALAD. 1 hit.
[Graphical view ]
PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterisation of the genes that encode enzymes for first steps of tetrapyrrole biosynthesis in Streptomyces coelicolor A3(2)."
    Petricek M.
    Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: A3(2) / NRRL B-16638.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.

Entry informationi

Entry nameiHEM2_STRCO
AccessioniPrimary (citable) accession number: P54919
Secondary accession number(s): Q9S2E6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 1, 2000
Last modified: May 14, 2014
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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