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P54919

- HEM2_STRCO

UniProt

P54919 - HEM2_STRCO

Protein

Delta-aminolevulinic acid dehydratase

Gene

hemB

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 2 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen By similarity.By similarity

    Catalytic activityi

    2 5-aminolevulinate = porphobilinogen + 2 H2O.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei203 – 2031Schiff-base intermediate with substrateBy similarity
    Binding sitei213 – 2131Substrate 1By similarity
    Binding sitei224 – 2241Substrate 1By similarity
    Metal bindingi240 – 2401MagnesiumBy similarity
    Active sitei255 – 2551Schiff-base intermediate with substrateBy similarity
    Binding sitei281 – 2811Substrate 2By similarity
    Binding sitei320 – 3201Substrate 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. porphobilinogen synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Heme biosynthesis, Porphyrin biosynthesis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00251; UER00318.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
    Short name:
    ALAD
    Short name:
    ALADH
    Alternative name(s):
    Porphobilinogen synthase
    Gene namesi
    Name:hemB
    Ordered Locus Names:SCO3311
    ORF Names:SCE68.09c
    OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
    Taxonomic identifieri100226 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
    ProteomesiUP000001973: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 330330Delta-aminolevulinic acid dehydratasePRO_0000140519Add
    BLAST

    Proteomic databases

    PRIDEiP54919.

    Interactioni

    Subunit structurei

    Homooctamer.By similarity

    Protein-protein interaction databases

    STRINGi100226.SCO3311.

    Structurei

    3D structure databases

    ProteinModelPortaliP54919.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ALADH family.Curated

    Phylogenomic databases

    eggNOGiCOG0113.
    HOGENOMiHOG000020323.
    KOiK01698.
    OMAiDMILTYF.
    OrthoDBiEOG6VXFCB.
    PhylomeDBiP54919.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR001731. Porphobilinogen_synth.
    [Graphical view]
    PANTHERiPTHR11458. PTHR11458. 1 hit.
    PfamiPF00490. ALAD. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
    PRINTSiPR00144. DALDHYDRTASE.
    SMARTiSM01004. ALAD. 1 hit.
    [Graphical view]
    PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P54919-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTTYGSFPGA RPRRLRTTPV MRRMVAETRL HPADFILPAF VREGVSEPVP    50
    IAAMPGVVQH TRDTLKKAAA EAVEAGVSGI MLFGVPEDGK KDAAGTAGTD 100
    PDGILQVALR DVRAEVGDEL LVMSDLCLDE FTDHGHCGVL DGQGRVDNDA 150
    TLERYAEMAQ VQADAGAHVV GPSGMMDGQI GVIRDALDQI GREDVAILAY 200
    TAKYASAFYG PFREAVGSSL KGDRKTYQQD SANARESLRE LALDLEEGAD 250
    MVMVKPAGPY LDILAKVAEA SDVPVAAYQI SGEYSMIEAA AEKGWIDRDR 300
    AILESLTGIK RAGARNILTY WATEVARTLR 330
    Length:330
    Mass (Da):35,453
    Last modified:December 1, 2000 - v2
    Checksum:iD2AF1F8B971F30F5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti100 – 1001D → V in AAA61398. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U19249 Genomic DNA. Translation: AAA61398.1.
    AL939116 Genomic DNA. Translation: CAB45345.1.
    PIRiT36259.
    RefSeqiNP_627521.1. NC_003888.3.

    Genome annotation databases

    EnsemblBacteriaiCAB45345; CAB45345; CAB45345.
    GeneIDi1098745.
    KEGGisco:SCO3311.
    PATRICi23736366. VBIStrCoe124346_3371.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U19249 Genomic DNA. Translation: AAA61398.1 .
    AL939116 Genomic DNA. Translation: CAB45345.1 .
    PIRi T36259.
    RefSeqi NP_627521.1. NC_003888.3.

    3D structure databases

    ProteinModelPortali P54919.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 100226.SCO3311.

    Proteomic databases

    PRIDEi P54919.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB45345 ; CAB45345 ; CAB45345 .
    GeneIDi 1098745.
    KEGGi sco:SCO3311.
    PATRICi 23736366. VBIStrCoe124346_3371.

    Phylogenomic databases

    eggNOGi COG0113.
    HOGENOMi HOG000020323.
    KOi K01698.
    OMAi DMILTYF.
    OrthoDBi EOG6VXFCB.
    PhylomeDBi P54919.

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00318 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR001731. Porphobilinogen_synth.
    [Graphical view ]
    PANTHERi PTHR11458. PTHR11458. 1 hit.
    Pfami PF00490. ALAD. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001415. Porphbilin_synth. 1 hit.
    PRINTSi PR00144. DALDHYDRTASE.
    SMARTi SM01004. ALAD. 1 hit.
    [Graphical view ]
    PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterisation of the genes that encode enzymes for first steps of tetrapyrrole biosynthesis in Streptomyces coelicolor A3(2)."
      Petricek M.
      Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: A3(2) / NRRL B-16638.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-471 / A3(2) / M145.

    Entry informationi

    Entry nameiHEM2_STRCO
    AccessioniPrimary (citable) accession number: P54919
    Secondary accession number(s): Q9S2E6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 92 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3