P54918 (ALR_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase
EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	slr0827

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis › Synechocystis sp. PCC 6803

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids By similarity. HAMAP-Rule MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP-Rule MF_01201
Cofactor	Pyridoxal phosphate By similarity. HAMAP-Rule MF_01201
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP-Rule MF_01201
Sequence similarities	Belongs to the alanine racemase family.
Sequence caution	The sequence BAA10510.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	D-alanine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Alanine racemase HAMAP-Rule MF_01201		PRO_0000114587
Sites
	Active site	37	1	Proton acceptor; specific for D-alanine By similarity
	Active site	265	1	Proton acceptor; specific for L-alanine By similarity
	Binding site	136	1	Substrate By similarity
	Binding site	313	1	Substrate; via amide nitrogen By similarity
Amino acid modifications
	Modified residue	37	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P54918 [UniParc]. Last modified March 5, 2002. Version 2. Checksum: 130C355A2835087B	FASTA	372	40,673
	10 20 30 40 50 60 MIRQRAWVEI DQAALVHNVR QFRQYVGPKT NLMAVVKADA YGHGAVRVAQ TALQAGADWL 70 80 90 100 110 120 AIATLGEGIE LREAGITAPI LLLGGINSPE EIEAIAHWRL QPTLCSPEQA QLFNDILLKL 130 140 150 160 170 180 GKVLPVHLKL DTGMTRLGTP WPQAANFVGL VQSLPQLRLA SLYSHLATAD DPNTATMLQQ 190 200 210 220 230 240 QERFAKAIAS LRQARLPIPK LHLANSAATL HGQAWHYDMV RVGLGLYGLY PAPHLGDCLD 250 260 270 280 290 300 LKPVLTVRAK ITQIRTIPPG TGVSYGHQFV SEETMPMAVV GIGYADGVPR NLSNQLEVLL 310 320 330 340 350 360 RGQPVRQIGA ITMDQMMVDL RGIDDPQVGE VVTLIGQDGD RQITADHWAS TLGTISWEIL 370 CGFKHRLPRI LI

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.

[2]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA10510.1. Different initiation.
PIR	S75775.
RefSeq	NP_442440.1. NC_000911.1. YP_005652501.1. NC_017277.1. YP_007452316.1. NC_020286.1.
3D structure databases
ProteinModelPortal	P54918.
ModBase	Search...
Protein-protein interaction databases
STRING	1148.slr0827.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA10510; BAA10510; BAA10510.
GeneID	12253813. 14617997. 954380.
KEGG	syn:slr0827. syy:SYNGTS_2548.
PATRIC	23842580. VBISynSp132158_2829.
Phylogenomic databases
eggNOG	COG0787.
HOGENOM	HOG000031444.
KO	K01775.
OMA	GQWQDIA.
ProtClustDB	PRK00053.
Enzyme and pathway databases
UniPathway	UPA00042; UER00497.
Family and domain databases
Gene3D	2.40.37.10. 1 hit.
HAMAP	MF_01201. Ala_racemase.
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_SYNY3

Accession

Primary (citable) accession number: P54918

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	March 5, 2002
Last modified:	May 29, 2013
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program