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P54905 (CRTB_RHOS4) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phytoene synthase

Short name=PSase
EC=2.5.1.-
Gene names
Name:crtB
Ordered Locus Names:RHOS4_18750
ORF Names:RSP_0270
OrganismRhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158) [Reference proteome] [HAMAP]
Taxonomic identifier272943 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter

Protein attributes

Sequence length355 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the biosynthesis of carotenoids. Catalyzes the condensation of two molecules of geranylgeranyl diphosphate (GGPP) to give prephytoene diphosphate (PPPP) and the subsequent rearrangement of the cyclopropylcarbinyl intermediate to yield phytoene By similarity.

Cofactor

ATP. ATP is required for the transferase activity but it does not seem to be hydrolyzed during the reaction By similarity.

Manganese or magnesium By similarity.

Pathway

Carotenoid biosynthesis; phytoene biosynthesis.

Sequence similarities

Belongs to the phytoene/squalene synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 355355Phytoene synthase
PRO_0000067434

Experimental info

Sequence conflict2191L → Q in AAF24290. Ref.3
Sequence conflict2861A → S Ref.1
Sequence conflict2861A → S Ref.2
Sequence conflict3241G → R in AAF24290. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P54905 [UniParc].

Last modified January 24, 2006. Version 2.
Checksum: F5437EBDBF661825

FASTA35538,987
        10         20         30         40         50         60 
MIASADLDAC REMIRTGSYS FHAASRLLPE RVRAPSLALY AFCRVADDAV DEAVNDGQRE 

        70         80         90        100        110        120 
EDAEVKRRAV LSLRDRLDLV YGGRPRNAPA DRAFAAVVEE FEMPRALPEA LLEGLAWDAV 

       130        140        150        160        170        180 
GRSYDSFSGV LDYSARVAAA VGAMMCVLMR VRDPDVLARA CDLGLAMQLT NIARDVGTDA 

       190        200        210        220        230        240 
RSGRIYLPRD WMEEEGLPVE EFLARPVVDD RIRAVTHRLL RAADRLYLRS EAGVCGLPLA 

       250        260        270        280        290        300 
CRPGIYAARH IYAGIGDEIA RNGYDSVTRR AFTTRRQKLV WLGLSATRAA LSPFGPGCAT 

       310        320        330        340        350 
LHAAPEPEVA FLVNAAARAR PQRGRSEALI SVLAQLEAQD RQISRQRLGN RANPI 

« Hide

References

« Hide 'large scale' references
[1]"Early steps in carotenoid biosynthesis: sequences and transcriptional analysis of the crtI and crtB genes of Rhodobacter sphaeroides and overexpression and reactivation of crtI in Escherichia coli and R. sphaeroides."
Lang H.P., Cogdell R.J., Gardiner A.T., Hunter C.N.
J. Bacteriol. 176:3859-3869(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete DNA sequence, specific Tn5 insertion map, and gene assignment of the carotenoid biosynthesis pathway of Rhodobacter sphaeroides."
Lang H.P., Cogdell R.J., Takaichi S., Hunter C.N.
J. Bacteriol. 177:2064-2073(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"DNA sequence analysis of the photosynthesis region of Rhodobacter sphaeroides 2.4.1."
Choudhary M., Kaplan S.
Nucleic Acids Res. 28:862-867(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C., Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S71770 Genomic DNA. Translation: AAB31139.1.
AJ010302 Genomic DNA. Translation: CAB38740.1.
AF195122 Genomic DNA. Translation: AAF24290.1.
CP000143 Genomic DNA. Translation: ABA79443.1.
PIRS49621.
T50746.
RefSeqYP_353344.1. NC_007493.2.

3D structure databases

ProteinModelPortalP54905.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272943.RSP_0270.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABA79443; ABA79443; RSP_0270.
GeneID3719279.
KEGGrsp:RSP_0270.
PATRIC23153676. VBIRhoSph57909_2213.

Phylogenomic databases

eggNOGCOG1562.
HOGENOMHOG000220848.
KOK02291.
OMAMMTLVMG.
OrthoDBEOG6QP0ZB.
ProtClustDBCLSK924105.

Enzyme and pathway databases

BioCycRSPH272943:GJAS-1927-MONOMER.
UniPathwayUPA00799.

Family and domain databases

Gene3D1.10.600.10. 1 hit.
InterProIPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMSSF48576. SSF48576. 1 hit.
PROSITEPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCRTB_RHOS4
AccessionPrimary (citable) accession number: P54905
Secondary accession number(s): Q3J191, Q9RFC9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 24, 2006
Last modified: January 22, 2014
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways