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Protein

Phytoene synthase

Gene

crtB

Organism
Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of carotenoids. Catalyzes the condensation of two molecules of geranylgeranyl diphosphate (GGPP) to give prephytoene diphosphate (PPPP) and the subsequent rearrangement of the cyclopropylcarbinyl intermediate to yield phytoene (By similarity).By similarity

Cofactori

Protein has several cofactor binding sites:
  • ATPBy similarityNote: ATP is required for the transferase activity but it does not seem to be hydrolyzed during the reaction.By similarity
  • Mn2+By similarity, Mg2+By similarity

Pathway:iphytoene biosynthesis

This protein is involved in the pathway phytoene biosynthesis, which is part of Carotenoid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway phytoene biosynthesis and in Carotenoid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Carotenoid biosynthesis

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciRSPH272943:GJAS-1927-MONOMER.
UniPathwayiUPA00799.

Names & Taxonomyi

Protein namesi
Recommended name:
Phytoene synthase (EC:2.5.1.-)
Short name:
PSase
Gene namesi
Name:crtB
Ordered Locus Names:RHOS4_18750
ORF Names:RSP_0270
OrganismiRhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Taxonomic identifieri272943 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
ProteomesiUP000002703 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 355355Phytoene synthasePRO_0000067434Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272943.RSP_0270.

Structurei

3D structure databases

ProteinModelPortaliP54905.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phytoene/squalene synthase family.Curated

Phylogenomic databases

eggNOGiCOG1562.
HOGENOMiHOG000220848.
KOiK02291.
OMAiEINKQWK.
OrthoDBiEOG6QP0ZB.
PhylomeDBiP54905.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
[Graphical view]
PfamiPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
PROSITEiPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54905-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIASADLDAC REMIRTGSYS FHAASRLLPE RVRAPSLALY AFCRVADDAV
60 70 80 90 100
DEAVNDGQRE EDAEVKRRAV LSLRDRLDLV YGGRPRNAPA DRAFAAVVEE
110 120 130 140 150
FEMPRALPEA LLEGLAWDAV GRSYDSFSGV LDYSARVAAA VGAMMCVLMR
160 170 180 190 200
VRDPDVLARA CDLGLAMQLT NIARDVGTDA RSGRIYLPRD WMEEEGLPVE
210 220 230 240 250
EFLARPVVDD RIRAVTHRLL RAADRLYLRS EAGVCGLPLA CRPGIYAARH
260 270 280 290 300
IYAGIGDEIA RNGYDSVTRR AFTTRRQKLV WLGLSATRAA LSPFGPGCAT
310 320 330 340 350
LHAAPEPEVA FLVNAAARAR PQRGRSEALI SVLAQLEAQD RQISRQRLGN

RANPI
Length:355
Mass (Da):38,987
Last modified:January 24, 2006 - v2
Checksum:iF5437EBDBF661825
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti219 – 2191L → Q in AAF24290 (PubMed:10648776).Curated
Sequence conflicti286 – 2861A → S (PubMed:8021167).Curated
Sequence conflicti286 – 2861A → S (PubMed:7721699).Curated
Sequence conflicti324 – 3241G → R in AAF24290 (PubMed:10648776).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S71770 Genomic DNA. Translation: AAB31139.1.
AJ010302 Genomic DNA. Translation: CAB38740.1.
AF195122 Genomic DNA. Translation: AAF24290.1.
CP000143 Genomic DNA. Translation: ABA79443.1.
PIRiS49621.
T50746.
RefSeqiWP_011338116.1. NZ_AKVW01000001.1.
YP_353344.1. NC_007493.2.

Genome annotation databases

EnsemblBacteriaiABA79443; ABA79443; RSP_0270.
GeneIDi3719279.
KEGGirsp:RSP_0270.
PATRICi23153676. VBIRhoSph57909_2213.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S71770 Genomic DNA. Translation: AAB31139.1.
AJ010302 Genomic DNA. Translation: CAB38740.1.
AF195122 Genomic DNA. Translation: AAF24290.1.
CP000143 Genomic DNA. Translation: ABA79443.1.
PIRiS49621.
T50746.
RefSeqiWP_011338116.1. NZ_AKVW01000001.1.
YP_353344.1. NC_007493.2.

3D structure databases

ProteinModelPortaliP54905.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272943.RSP_0270.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABA79443; ABA79443; RSP_0270.
GeneIDi3719279.
KEGGirsp:RSP_0270.
PATRICi23153676. VBIRhoSph57909_2213.

Phylogenomic databases

eggNOGiCOG1562.
HOGENOMiHOG000220848.
KOiK02291.
OMAiEINKQWK.
OrthoDBiEOG6QP0ZB.
PhylomeDBiP54905.

Enzyme and pathway databases

UniPathwayiUPA00799.
BioCyciRSPH272943:GJAS-1927-MONOMER.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
[Graphical view]
PfamiPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
PROSITEiPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Early steps in carotenoid biosynthesis: sequences and transcriptional analysis of the crtI and crtB genes of Rhodobacter sphaeroides and overexpression and reactivation of crtI in Escherichia coli and R. sphaeroides."
    Lang H.P., Cogdell R.J., Gardiner A.T., Hunter C.N.
    J. Bacteriol. 176:3859-3869(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete DNA sequence, specific Tn5 insertion map, and gene assignment of the carotenoid biosynthesis pathway of Rhodobacter sphaeroides."
    Lang H.P., Cogdell R.J., Takaichi S., Hunter C.N.
    J. Bacteriol. 177:2064-2073(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "DNA sequence analysis of the photosynthesis region of Rhodobacter sphaeroides 2.4.1."
    Choudhary M., Kaplan S.
    Nucleic Acids Res. 28:862-867(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C., Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158.

Entry informationi

Entry nameiCRTB_RHOS4
AccessioniPrimary (citable) accession number: P54905
Secondary accession number(s): Q3J191, Q9RFC9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 24, 2006
Last modified: July 22, 2015
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.