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P54905

- CRTB_RHOS4

UniProt

P54905 - CRTB_RHOS4

Protein

Phytoene synthase

Gene

crtB

Organism
Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 2 (24 Jan 2006)
      Previous versions | rss
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    Functioni

    Involved in the biosynthesis of carotenoids. Catalyzes the condensation of two molecules of geranylgeranyl diphosphate (GGPP) to give prephytoene diphosphate (PPPP) and the subsequent rearrangement of the cyclopropylcarbinyl intermediate to yield phytoene By similarity.By similarity

    Cofactori

    ATP. ATP is required for the transferase activity but it does not seem to be hydrolyzed during the reaction By similarity.By similarity
    Manganese or magnesium.By similarity

    Pathwayi

    GO - Molecular functioni

    1. geranylgeranyl-diphosphate geranylgeranyltransferase activity Source: UniProtKB
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. carotenoid biosynthetic process Source: UniProtKB

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Carotenoid biosynthesis

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciRSPH272943:GJAS-1927-MONOMER.
    UniPathwayiUPA00799.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phytoene synthase (EC:2.5.1.-)
    Short name:
    PSase
    Gene namesi
    Name:crtB
    Ordered Locus Names:RHOS4_18750
    ORF Names:RSP_0270
    OrganismiRhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
    Taxonomic identifieri272943 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
    ProteomesiUP000002703: Chromosome 1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 355355Phytoene synthasePRO_0000067434Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi272943.RSP_0270.

    Structurei

    3D structure databases

    ProteinModelPortaliP54905.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the phytoene/squalene synthase family.Curated

    Phylogenomic databases

    eggNOGiCOG1562.
    HOGENOMiHOG000220848.
    KOiK02291.
    OMAiMMTLVMG.
    OrthoDBiEOG6QP0ZB.
    PhylomeDBiP54905.

    Family and domain databases

    Gene3Di1.10.600.10. 1 hit.
    InterProiIPR002060. Squ/phyt_synthse.
    IPR019845. Squalene/phytoene_synthase_CS.
    IPR008949. Terpenoid_synth.
    [Graphical view]
    PfamiPF00494. SQS_PSY. 1 hit.
    [Graphical view]
    SUPFAMiSSF48576. SSF48576. 1 hit.
    PROSITEiPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
    PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P54905-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIASADLDAC REMIRTGSYS FHAASRLLPE RVRAPSLALY AFCRVADDAV    50
    DEAVNDGQRE EDAEVKRRAV LSLRDRLDLV YGGRPRNAPA DRAFAAVVEE 100
    FEMPRALPEA LLEGLAWDAV GRSYDSFSGV LDYSARVAAA VGAMMCVLMR 150
    VRDPDVLARA CDLGLAMQLT NIARDVGTDA RSGRIYLPRD WMEEEGLPVE 200
    EFLARPVVDD RIRAVTHRLL RAADRLYLRS EAGVCGLPLA CRPGIYAARH 250
    IYAGIGDEIA RNGYDSVTRR AFTTRRQKLV WLGLSATRAA LSPFGPGCAT 300
    LHAAPEPEVA FLVNAAARAR PQRGRSEALI SVLAQLEAQD RQISRQRLGN 350
    RANPI 355
    Length:355
    Mass (Da):38,987
    Last modified:January 24, 2006 - v2
    Checksum:iF5437EBDBF661825
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti219 – 2191L → Q in AAF24290. (PubMed:10648776)Curated
    Sequence conflicti286 – 2861A → S(PubMed:8021167)Curated
    Sequence conflicti286 – 2861A → S(PubMed:7721699)Curated
    Sequence conflicti324 – 3241G → R in AAF24290. (PubMed:10648776)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S71770 Genomic DNA. Translation: AAB31139.1.
    AJ010302 Genomic DNA. Translation: CAB38740.1.
    AF195122 Genomic DNA. Translation: AAF24290.1.
    CP000143 Genomic DNA. Translation: ABA79443.1.
    PIRiS49621.
    T50746.
    RefSeqiWP_011338116.1. NZ_AKVW01000001.1.
    YP_353344.1. NC_007493.2.

    Genome annotation databases

    EnsemblBacteriaiABA79443; ABA79443; RSP_0270.
    GeneIDi3719279.
    KEGGirsp:RSP_0270.
    PATRICi23153676. VBIRhoSph57909_2213.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S71770 Genomic DNA. Translation: AAB31139.1 .
    AJ010302 Genomic DNA. Translation: CAB38740.1 .
    AF195122 Genomic DNA. Translation: AAF24290.1 .
    CP000143 Genomic DNA. Translation: ABA79443.1 .
    PIRi S49621.
    T50746.
    RefSeqi WP_011338116.1. NZ_AKVW01000001.1.
    YP_353344.1. NC_007493.2.

    3D structure databases

    ProteinModelPortali P54905.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272943.RSP_0270.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABA79443 ; ABA79443 ; RSP_0270 .
    GeneIDi 3719279.
    KEGGi rsp:RSP_0270.
    PATRICi 23153676. VBIRhoSph57909_2213.

    Phylogenomic databases

    eggNOGi COG1562.
    HOGENOMi HOG000220848.
    KOi K02291.
    OMAi MMTLVMG.
    OrthoDBi EOG6QP0ZB.
    PhylomeDBi P54905.

    Enzyme and pathway databases

    UniPathwayi UPA00799 .
    BioCyci RSPH272943:GJAS-1927-MONOMER.

    Family and domain databases

    Gene3Di 1.10.600.10. 1 hit.
    InterProi IPR002060. Squ/phyt_synthse.
    IPR019845. Squalene/phytoene_synthase_CS.
    IPR008949. Terpenoid_synth.
    [Graphical view ]
    Pfami PF00494. SQS_PSY. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48576. SSF48576. 1 hit.
    PROSITEi PS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
    PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Early steps in carotenoid biosynthesis: sequences and transcriptional analysis of the crtI and crtB genes of Rhodobacter sphaeroides and overexpression and reactivation of crtI in Escherichia coli and R. sphaeroides."
      Lang H.P., Cogdell R.J., Gardiner A.T., Hunter C.N.
      J. Bacteriol. 176:3859-3869(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete DNA sequence, specific Tn5 insertion map, and gene assignment of the carotenoid biosynthesis pathway of Rhodobacter sphaeroides."
      Lang H.P., Cogdell R.J., Takaichi S., Hunter C.N.
      J. Bacteriol. 177:2064-2073(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "DNA sequence analysis of the photosynthesis region of Rhodobacter sphaeroides 2.4.1."
      Choudhary M., Kaplan S.
      Nucleic Acids Res. 28:862-867(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1."
      Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C., Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158.

    Entry informationi

    Entry nameiCRTB_RHOS4
    AccessioniPrimary (citable) accession number: P54905
    Secondary accession number(s): Q3J191, Q9RFC9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: January 24, 2006
    Last modified: October 1, 2014
    This is version 89 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3