P54905 (CRTB_RHOS4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phytoene synthase
EC=2.5.1.32

Gene names

Name:	crtB
Ordered Locus Names:	RHOS4_18750
ORF Names:	RSP_0270

Organism

Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158) [Reference proteome] [HAMAP]

Taxonomic identifier

272943 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter › Rhodobacter sphaeroides

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reaction from prephytoene diphosphate to phytoene.
Catalytic activity	2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.
Pathway	Carotenoid biosynthesis; phytoene biosynthesis; all-trans-phytoene from geranylgeranyl diphosphate: step 1/1.
Sequence similarities	Belongs to the phytoene/squalene synthase family.

Ontologies

Keywords
Biological process	Carotenoid biosynthesis Chlorophyll biosynthesis
Molecular function	Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	carotenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	geranylgeranyl-diphosphate geranylgeranyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 355	355	Phytoene synthase		PRO_0000067434
Experimental info
	Sequence conflict	219	1	L → Q in AAF24290. Ref.3
	Sequence conflict	286	1	A → S Ref.1
	Sequence conflict	286	1	A → S Ref.2
	Sequence conflict	324	1	G → R in AAF24290. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P54905 [UniParc]. Last modified January 24, 2006. Version 2. Checksum: F5437EBDBF661825	FASTA	355	38,987
	10 20 30 40 50 60 MIASADLDAC REMIRTGSYS FHAASRLLPE RVRAPSLALY AFCRVADDAV DEAVNDGQRE 70 80 90 100 110 120 EDAEVKRRAV LSLRDRLDLV YGGRPRNAPA DRAFAAVVEE FEMPRALPEA LLEGLAWDAV 130 140 150 160 170 180 GRSYDSFSGV LDYSARVAAA VGAMMCVLMR VRDPDVLARA CDLGLAMQLT NIARDVGTDA 190 200 210 220 230 240 RSGRIYLPRD WMEEEGLPVE EFLARPVVDD RIRAVTHRLL RAADRLYLRS EAGVCGLPLA 250 260 270 280 290 300 CRPGIYAARH IYAGIGDEIA RNGYDSVTRR AFTTRRQKLV WLGLSATRAA LSPFGPGCAT 310 320 330 340 350 LHAAPEPEVA FLVNAAARAR PQRGRSEALI SVLAQLEAQD RQISRQRLGN RANPI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Early steps in carotenoid biosynthesis: sequences and transcriptional analysis of the crtI and crtB genes of Rhodobacter sphaeroides and overexpression and reactivation of crtI in Escherichia coli and R. sphaeroides." Lang H.P., Cogdell R.J., Gardiner A.T., Hunter C.N. J. Bacteriol. 176:3859-3869(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete DNA sequence, specific Tn5 insertion map, and gene assignment of the carotenoid biosynthesis pathway of Rhodobacter sphaeroides." Lang H.P., Cogdell R.J., Takaichi S., Hunter C.N. J. Bacteriol. 177:2064-2073(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"DNA sequence analysis of the photosynthesis region of Rhodobacter sphaeroides 2.4.1." Choudhary M., Kaplan S. Nucleic Acids Res. 28:862-867(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C., Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S71770 Genomic DNA. Translation: AAB31139.1. AJ010302 Genomic DNA. Translation: CAB38740.1. AF195122 Genomic DNA. Translation: AAF24290.1. CP000143 Genomic DNA. Translation: ABA79443.1.
PIR	S49621. T50746.
RefSeq	YP_353344.1. NC_007493.1.
3D structure databases
ProteinModelPortal	P54905.
ModBase	Search...
Protein-protein interaction databases
STRING	272943.RSP_0270.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3719279.
KEGG	rsp:RSP_0270.
PATRIC	23153676. VBIRhoSph57909_2213.
Phylogenomic databases
eggNOG	COG1562.
HOGENOM	HOG000220848.
KO	K02291.
OMA	MMTLVMG.
ProtClustDB	CLSK924105.
Enzyme and pathway databases
BioCyc	RSPH272943:GJAS-1927-MONOMER.
UniPathway	UPA00799; UER00773.
Family and domain databases
Gene3D	1.10.600.10. 1 hit.
InterPro	IPR002060. Squ/phyt_synthse. IPR019845. Squalene/phytoene_synthase_CS. IPR008949. Terpenoid_synth. [Graphical view]
Pfam	PF00494. SQS_PSY. 1 hit. [Graphical view]
SUPFAM	SSF48576. Terpenoid_synth. 1 hit.
PROSITE	PS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit. PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CRTB_RHOS4

Accession

Primary (citable) accession number: P54905
Secondary accession number(s): Q3J191, Q9RFC9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 24, 2006
Last modified:	May 1, 2013
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program