P54903 (PROA_THET2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	TT_C1564

Organism

Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) [Complete proteome] [HAMAP]

Taxonomic identifier

262724 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	413 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.
Sequence caution	The sequence BAA06238.1 differs from that shown. Reason: Frameshift at position 389.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 413	413	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_0000189803

Sequences

	Sequence		Length	Mass (Da)
	P54903 [UniParc]. Last modified May 24, 2004. Version 3. Checksum: EC63FDFF0FC2B71E	FASTA	413	45,851
	10 20 30 40 50 60 MTATSLWELA ERARKRLSEI AKGNRDRALL AMADLLEARW EEVLRANRED LEEAERTGLP 70 80 90 100 110 120 KAKLDRLALK EKDLKTLTEG LRQIARLPDP LGRIEGLAKR PNGLRVGRMR VPLGLIGFIY 130 140 150 160 170 180 EARPGATVEA VSVALKAGNA MLLRGGKEAF RSNRALVALW HEALEEAGLP EEAVTLVPTT 190 200 210 220 230 240 DREAVLEMCR LELLDLLIPR GGEELIRLVQ QEARVPVLAH AKGVNHLYVD EKADLSMALR 250 260 270 280 290 300 LALNGKTQRP AVCNALEAVL VHEKVAEAFL PRLEKAMREK GVELRACPRA LPLLKEAVPA 310 320 330 340 350 360 REDEWDREYL DLVLRVKVVS GLEEALAHIA RYGSRHTEAI CTEDPKAAWR FLEEVDASLV 370 380 390 400 410 LWNASTRFND GFELGLGAEI GISTSKLHAY GPMGPLELTT LKWVALGEGQ ERT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and sequence analysis of the proBA operon from an extremely thermophilic eubacterium Thermus thermophilus." Kosuge T., Tabata K., Hoshino T. FEMS Microbiol. Lett. 123:55-61(1994) [PubMed: 7988899] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of the extreme thermophile Thermus thermophilus." Henne A., Brueggemann H., Raasch C., Wiezer A., Hartsch T., Liesegang H., Johann A., Lienard T., Gohl O., Martinez-Arias R., Jacobi C., Starkuviene V., Schlenczeck S., Dencker S., Huber R., Klenk H.-P., Kramer W., Merkl R., Gottschalk G., Fritz H.-J. Nat. Biotechnol. 22:547-553(2004) [PubMed: 15064768] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HB27 / ATCC BAA-163 / DSM 7039.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D29973 Genomic DNA. Translation: BAA06238.1. Frameshift. AE017221 Genomic DNA. Translation: AAS81906.1.
RefSeq	YP_005533.1. NC_005835.1.
3D structure databases
ProteinModelPortal	P54903.
ModBase	Search...
Protein-protein interaction databases
STRING	P54903.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2775994.
GenomeReviews	Gene locus TT_C1564 in contig AE017221_GR.
PATRIC	23953565. VBITheThe54392_1560.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0014.
HOGENOM	HBG318080.
OMA	YGICGAM.
PhylomeDB	P54903.
ProtClustDB	PRK00197.
Enzyme and pathway databases
BioCyc	TTHE262724:TT_C1564-MONOMER.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 2 hits.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. ProA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_THET2

Accession

Primary (citable) accession number: P54903

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	May 24, 2004
Last modified:	January 25, 2012
This is version 87 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program