Reviewed, UniProtKB/Swiss-Prot P54902 (PROA_SYNY3)

Names and origin

Protein names

Recommended name:
    Probable gamma-glutamyl phosphate reductase
      Short name=GPR
    EC=1.2.1.41
Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
      Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	sll0373

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	433 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 433	433	Probable gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_0000189801

Sequences

	Sequence		Length	Mass (Da)
	P54902-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 41C590A10DC90283	FASTA	433	46,350
	10 20 30 40 50 60 MVISPSLSAL HTLAHNARQA ALHLGSLNTA ERNQAIAAIA DGLTAAMPEI LAANQEDCAA 70 80 90 100 110 120 AEAMGIAKPL YNRLLLGESK LKSTIAGVKD VEHLPDPLGQ VTLHRQLDEG LVLKRVGCPL 130 140 150 160 170 180 GVLGVIFEAR PEALIQISSL AIKSGNAVIL KGGREATRSC QVLTEVIQTA LAKTVVSPEA 190 200 210 220 230 240 INLLTTREEI RELLGLNQYV DLIIPRGSNE FVQYIQQNTQ IPVLGHADGI CHLYLDAQAD 250 260 270 280 290 300 LSKAIPITVD AKTQYPAACN AIETLLVHQA IAAEFLPPLA QALGEKGVSL RGDSGTQKLI 310 320 330 340 350 360 DCEPATEADW CTEYSDLILS IKIVDSLEAA IDHINQYGSK HTDGIISEDL TAAEQFCQRV 370 380 390 400 410 420 DSAGVYHNCS TRFADGFRYG FGAEVGISTQ KMPPRGPVGL EGLVTYKYQL WGHGHIAATY 430 TGQGAKAFTH LDL

References

[1]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed: 8590279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000022 Genomic DNA. Translation: BAA10399.1.
PIR	S76553.
RefSeq	NP_442329.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P54902.
Genome annotation databases
GeneID	952780.
GenomeReviews	Gene locus sll0373 in contig BA000022_GR.
KEGG	syn:sll0373.
NMPDR	fig|1148.1.peg.2430.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P54902.
OMA	QYPAACN
Enzyme and pathway databases
BioCyc	SSP1148:SLL0373-MON.
Family and domain databases
HAMAP	MF_00412. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_SYNY3

Accession

Primary (citable) accession number: P54902

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 24, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)