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P54897 (DAPF1_NOSS1) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Diaminopimelate epimerase 1

Short name=DAP epimerase 1
EC=5.1.1.7
Gene names
Name:dapF1
Synonyms:dapF
Ordered Locus Names:alr4841
OrganismNostoc sp. (strain PCC 7120 / UTEX 2576) [Complete proteome] [HAMAP]
Taxonomic identifier103690 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaNostocalesNostocaceaeNostoc

Protein attributes

Sequence length285 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan By similarity. HAMAP-Rule MF_00197

Catalytic activity

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate. HAMAP-Rule MF_00197

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from LL-2,6-diaminopimelate: step 1/1. HAMAP-Rule MF_00197

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00197.

Sequence similarities

Belongs to the diaminopimelate epimerase family.

Caution

Could lack activity as the potential active site Cys residues in positions 72 and 218 are both replaced by a Ser.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Lysine biosynthesis
   Cellular componentCytoplasm
   Molecular functionIsomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processlysine biosynthetic process via diaminopimelate

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiondiaminopimelate epimerase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 285285Diaminopimelate epimerase 1 HAMAP-Rule MF_00197
PRO_0000149816

Regions

Region8 – 92Substrate binding By similarity
Region209 – 2102Substrate binding By similarity

Sites

Active site721Proton donor/acceptor By similarity
Active site2181Proton donor/acceptor By similarity
Binding site111Substrate By similarity
Binding site441Substrate By similarity
Binding site631Substrate By similarity
Binding site1581Substrate By similarity
Binding site1911Substrate By similarity
Site1601Important for catalytic activity By similarity
Site2091Important for catalytic activity By similarity

Experimental info

Sequence conflict2231Missing in CAA87006. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P54897 [UniParc].

Last modified January 23, 2002. Version 2.
Checksum: AE1613FFEAD75063

FASTA28531,355
        10         20         30         40         50         60 
MKFYKYHALG NDYLVINPQD LKFPLTPEKI KIICHRNFGI GSDGILLGPL ASTKAQFALR 

        70         80         90        100        110        120 
IFNPDGSEAE KSGNGLRIFS RYLWDMGLVD EKPFSIETAG GIVESAIKDA GKTVQVEMGK 

       130        140        150        160        170        180 
VSFWSRDIPV IGDDREVIQE KIFLDEGIFT FCAATIGNPH CVILLDDINS EVARNFGPIF 

       190        200        210        220        230        240 
EGYPLFPNRT NVQFMKVLDR STIQIEIWER GAGYTLASGS SSSAAAATAH KLGLCDSKII 

       250        260        270        280 
VKMPGGDILI QIKDDFHISM TDSVTKLAQG ELSTEIFAIE TVINI 

« Hide

References

« Hide 'large scale' references
[1]"Identification and characterization of the nifV-nifZ-nifT gene region from the filamentous cyanobacterium Anabaena sp. strain PCC 7120."
Stricker O., Masepohl B., Klipp W., Boehme H.
J. Bacteriol. 179:2930-2937(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120."
Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. expand/collapse author list , Takazawa M., Yamada M., Yasuda M., Tabata S.
DNA Res. 8:205-213(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 7120 / UTEX 2576.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z46907 Genomic DNA. Translation: CAA87006.1.
BA000019 Genomic DNA. Translation: BAB76540.1.
PIRAI2410.
S52295.
RefSeqNP_488881.1. NC_003272.1.

3D structure databases

ProteinModelPortalP54897.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING103690.alr4841.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB76540; BAB76540; BAB76540.
GeneID1108442.
KEGGana:alr4841.
PATRIC22780337. VBINosSp37423_5611.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0253.
HOGENOMHOG000220466.
KOK01778.
OMARTNVQFM.
OrthoDBEOG6ND0M5.
ProtClustDBPRK13577.

Enzyme and pathway databases

UniPathwayUPA00034; UER00025.

Family and domain databases

HAMAPMF_00197. DAP_epimerase.
InterProIPR001653. DAP_epimerase_DapF.
[Graphical view]
PANTHERPTHR31689:SF0. PTHR31689:SF0. 1 hit.
PfamPF01678. DAP_epimerase. 2 hits.
[Graphical view]
TIGRFAMsTIGR00652. DapF. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDAPF1_NOSS1
AccessionPrimary (citable) accession number: P54897
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2002
Last modified: February 19, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways