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P54897

- DAPF1_NOSS1

UniProt

P54897 - DAPF1_NOSS1

Protein

Diaminopimelate epimerase 1

Gene

dapF1

Organism
Nostoc sp. (strain PCC 7120 / UTEX 2576)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 2 (23 Jan 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.By similarity

    Catalytic activityi

    LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei11 – 111SubstrateBy similarity
    Binding sitei44 – 441SubstrateBy similarity
    Binding sitei63 – 631SubstrateBy similarity
    Active sitei72 – 721Proton donor/acceptorBy similarity
    Binding sitei158 – 1581SubstrateBy similarity
    Sitei160 – 1601Important for catalytic activityBy similarity
    Binding sitei191 – 1911SubstrateBy similarity
    Sitei209 – 2091Important for catalytic activityBy similarity
    Active sitei218 – 2181Proton donor/acceptorBy similarity

    GO - Molecular functioni

    1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Amino-acid biosynthesis, Lysine biosynthesis

    Enzyme and pathway databases

    UniPathwayiUPA00034; UER00025.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diaminopimelate epimerase 1 (EC:5.1.1.7)
    Short name:
    DAP epimerase 1
    Gene namesi
    Name:dapF1
    Synonyms:dapF
    Ordered Locus Names:alr4841
    OrganismiNostoc sp. (strain PCC 7120 / UTEX 2576)
    Taxonomic identifieri103690 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
    ProteomesiUP000002483: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 285285Diaminopimelate epimerase 1PRO_0000149816Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi103690.alr4841.

    Structurei

    3D structure databases

    ProteinModelPortaliP54897.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni8 – 92Substrate bindingBy similarity
    Regioni209 – 2102Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the diaminopimelate epimerase family.Curated

    Phylogenomic databases

    eggNOGiCOG0253.
    HOGENOMiHOG000220466.
    KOiK01778.
    OrthoDBiEOG6ND0M5.

    Family and domain databases

    HAMAPiMF_00197. DAP_epimerase.
    InterProiIPR001653. DAP_epimerase_DapF.
    [Graphical view]
    PANTHERiPTHR31689. PTHR31689. 1 hit.
    PfamiPF01678. DAP_epimerase. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR00652. DapF. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P54897-1 [UniParc]FASTAAdd to Basket

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    MKFYKYHALG NDYLVINPQD LKFPLTPEKI KIICHRNFGI GSDGILLGPL    50
    ASTKAQFALR IFNPDGSEAE KSGNGLRIFS RYLWDMGLVD EKPFSIETAG 100
    GIVESAIKDA GKTVQVEMGK VSFWSRDIPV IGDDREVIQE KIFLDEGIFT 150
    FCAATIGNPH CVILLDDINS EVARNFGPIF EGYPLFPNRT NVQFMKVLDR 200
    STIQIEIWER GAGYTLASGS SSSAAAATAH KLGLCDSKII VKMPGGDILI 250
    QIKDDFHISM TDSVTKLAQG ELSTEIFAIE TVINI 285
    Length:285
    Mass (Da):31,355
    Last modified:January 23, 2002 - v2
    Checksum:iAE1613FFEAD75063
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti223 – 2231Missing in CAA87006. (PubMed:9139910)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46907 Genomic DNA. Translation: CAA87006.1.
    BA000019 Genomic DNA. Translation: BAB76540.1.
    PIRiAI2410.
    S52295.
    RefSeqiNP_488881.1. NC_003272.1.
    WP_010998969.1. NC_003272.1.

    Genome annotation databases

    EnsemblBacteriaiBAB76540; BAB76540; BAB76540.
    GeneIDi1108442.
    KEGGiana:alr4841.
    PATRICi22780337. VBINosSp37423_5611.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46907 Genomic DNA. Translation: CAA87006.1 .
    BA000019 Genomic DNA. Translation: BAB76540.1 .
    PIRi AI2410.
    S52295.
    RefSeqi NP_488881.1. NC_003272.1.
    WP_010998969.1. NC_003272.1.

    3D structure databases

    ProteinModelPortali P54897.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 103690.alr4841.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB76540 ; BAB76540 ; BAB76540 .
    GeneIDi 1108442.
    KEGGi ana:alr4841.
    PATRICi 22780337. VBINosSp37423_5611.

    Phylogenomic databases

    eggNOGi COG0253.
    HOGENOMi HOG000220466.
    KOi K01778.
    OrthoDBi EOG6ND0M5.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00025 .

    Family and domain databases

    HAMAPi MF_00197. DAP_epimerase.
    InterProi IPR001653. DAP_epimerase_DapF.
    [Graphical view ]
    PANTHERi PTHR31689. PTHR31689. 1 hit.
    Pfami PF01678. DAP_epimerase. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR00652. DapF. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Identification and characterization of the nifV-nifZ-nifT gene region from the filamentous cyanobacterium Anabaena sp. strain PCC 7120."
      Stricker O., Masepohl B., Klipp W., Boehme H.
      J. Bacteriol. 179:2930-2937(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PCC 7120 / UTEX 2576.

    Entry informationi

    Entry nameiDAPF1_NOSS1
    AccessioniPrimary (citable) accession number: P54897
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: January 23, 2002
    Last modified: October 1, 2014
    This is version 90 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Caution

    Could lack activity as the potential active site Cys residues in positions 72 and 218 are both replaced by a Ser.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3