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P54889

- ALH13_CAEEL

UniProt

P54889 - ALH13_CAEEL

Protein

Probable delta-1-pyrroline-5-carboxylate synthase

Gene

alh-13

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 2 (18 Sep 2013)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-glutamate = ADP + L-glutamate 5-phosphate.
    L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei128 – 1281SubstrateBy similarity
    Binding sitei227 – 2271SubstrateBy similarity
    Binding sitei242 – 2421Substrate; via amide nitrogenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi262 – 2632ATPBy similarity
    Nucleotide bindingi301 – 3077ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate 5-kinase activity Source: UniProtKB-EC
    3. glutamate-5-semialdehyde dehydrogenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. L-proline biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Oxidoreductase, Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Proline biosynthesis

    Keywords - Ligandi

    ATP-binding, NADP, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00098; UER00359.
    UPA00098; UER00360.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable delta-1-pyrroline-5-carboxylate synthase
    Short name:
    P5CS
    Including the following 2 domains:
    Glutamate 5-kinase (EC:2.7.2.11)
    Short name:
    GK
    Alternative name(s):
    Gamma-glutamyl kinase
    Gamma-glutamyl phosphate reductase (EC:1.2.1.41)
    Short name:
    GPR
    Alternative name(s):
    Aldehyde dehydrogenase family 13
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
    Gene namesi
    Name:alh-13
    ORF Names:T22H6.2
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome X

    Organism-specific databases

    WormBaseiT22H6.2a; CE47944; WBGene00011938; alh-13.
    T22H6.2b; CE47964; WBGene00011938; alh-13.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrion Source: WormBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 802802Probable delta-1-pyrroline-5-carboxylate synthasePRO_0000109771Add
    BLAST

    Proteomic databases

    PaxDbiP54889.
    PRIDEiP54889.

    Interactioni

    Protein-protein interaction databases

    STRINGi6239.T22H6.2b.

    Structurei

    3D structure databases

    ProteinModelPortaliP54889.
    SMRiP54889. Positions 81-345, 356-782.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 354354Glutamate 5-kinaseAdd
    BLAST
    Regioni355 – 802448Gamma-glutamyl phosphate reductaseAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the glutamate 5-kinase family.Curated
    In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.Curated

    Phylogenomic databases

    eggNOGiCOG0014.
    GeneTreeiENSGT00500000044903.
    HOGENOMiHOG000246357.
    KOiK12657.
    OMAiLLPWVQS.
    OrthoDBiEOG7WX07N.

    Family and domain databases

    Gene3Di3.40.1160.10. 1 hit.
    3.40.309.10. 1 hit.
    3.40.605.10. 2 hits.
    HAMAPiMF_00412. ProA.
    MF_00456. ProB.
    InterProiIPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR016162. Ald_DH_N.
    IPR015590. Aldehyde_DH_dom.
    IPR001048. Asp/Glu/Uridylate_kinase.
    IPR000965. G-glutamylP_reductase.
    IPR020593. G-glutamylP_reductase_CS.
    IPR001057. Glu/AcGlu_kinase.
    IPR005715. Glu_5kinase/COase_Synthase.
    IPR019797. Glutamate_5-kinase_CS.
    IPR005766. P5_carboxy_syn.
    [Graphical view]
    PfamiPF00696. AA_kinase. 1 hit.
    PF00171. Aldedh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036429. P5C_syn. 1 hit.
    PRINTSiPR00474. GLU5KINASE.
    SUPFAMiSSF53633. SSF53633. 1 hit.
    SSF53720. SSF53720. 1 hit.
    TIGRFAMsiTIGR01092. P5CS. 1 hit.
    TIGR00407. proA. 1 hit.
    TIGR01027. proB. 1 hit.
    PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
    PS01223. PROA. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform b (identifier: P54889-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MFRATRCLRL PLRNSHINIL RPTQTELIKT RSSALAPYEK VSPITAVGAT    50
    PVGVGNGNYC YSTKTRQKHP LINTRNDLKK AQRVVVKLGS AVITREDECG 100
    LALGRLASIV EQVSELQQSG RQMLIVSSGA VAFGRQKLRQ ELVMSMSMRQ 150
    TLRGPSGMTA DKRACAASGM PGLMSLYEQL FQQYGITVAQ VLLTKPDIDD 200
    DQRRKNLQAT IESLLSLNII PIVNANDAVA PDPKLNMHIS DNDSLAARLS 250
    AEIEAELLII LSNVNGVYTG PPDLEGSRLL YTYVPSENSG VTFGANSKFG 300
    TGGMESKVTA CVNALNNGVT TVITNGLAQD AITDAVAGKK IGTMFCNTKG 350
    YEGPPIEEVA EKCRDAGRQL AALSNKERGA MVRHLAALLV DKEKYIIEAN 400
    QTDLANAKSA GLDPQLLNRL KMTPEKIQDL HAGLNTIADS AETLVGRVLK 450
    KVKISEGLFL EQVTVPIGSL MVIFESRPDC LPQVASLAMA SGNALLLKGG 500
    KEAEESNKAL HALVQEALGT HGFEMRDAVT LVRSREDVAD LLQLKDLIDL 550
    IIPRGSSDLV RSMQEKSKGI PVLGHAEGVC HVYIDKDCDE QKAIQIVRDS 600
    KCDYPSACNA AETILIHKDL ATAPFFDSLC SMFKAEGVKL HAGPKLAALL 650
    KFAPPPAESM SFEYGSLECT LEVVDNVEEA VAHIIRYGSG HTESIITENT 700
    NTAEHFLKHV DSACAFHNAS TRFADGYRFG LGAEVGISTG RIHARGPVGV 750
    EGLLTTKWLL RGEGHLVEDF KNGKYSYLHE NLNPSEVYRA LDAAGELKKA 800
    TA 802

    Note: No experimental confirmation available.

    Length:802
    Mass (Da):86,694
    Last modified:September 18, 2013 - v2
    Checksum:i9342768572E138E9
    GO
    Isoform a (identifier: P54889-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         61-62: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:800
    Mass (Da):86,444
    Checksum:i287B2698EE2F5B88
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei61 – 622Missing in isoform a. CuratedVSP_047890

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z50797 Genomic DNA. Translation: CAA90672.2.
    Z50797 Genomic DNA. Translation: CAC35828.2.
    PIRiT25140.
    RefSeqiNP_510132.2. NM_077731.4. [P54889-1]
    NP_510133.2. NM_077732.6. [P54889-2]
    UniGeneiCel.17267.

    Genome annotation databases

    EnsemblMetazoaiT22H6.2b; T22H6.2b; WBGene00011938. [P54889-1]
    GeneIDi181417.
    KEGGicel:CELE_T22H6.2.
    UCSCiT22H6.2b. c. elegans. [P54889-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z50797 Genomic DNA. Translation: CAA90672.2 .
    Z50797 Genomic DNA. Translation: CAC35828.2 .
    PIRi T25140.
    RefSeqi NP_510132.2. NM_077731.4. [P54889-1 ]
    NP_510133.2. NM_077732.6. [P54889-2 ]
    UniGenei Cel.17267.

    3D structure databases

    ProteinModelPortali P54889.
    SMRi P54889. Positions 81-345, 356-782.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 6239.T22H6.2b.

    Proteomic databases

    PaxDbi P54889.
    PRIDEi P54889.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai T22H6.2b ; T22H6.2b ; WBGene00011938 . [P54889-1 ]
    GeneIDi 181417.
    KEGGi cel:CELE_T22H6.2.
    UCSCi T22H6.2b. c. elegans. [P54889-1 ]

    Organism-specific databases

    CTDi 181417.
    WormBasei T22H6.2a ; CE47944 ; WBGene00011938 ; alh-13.
    T22H6.2b ; CE47964 ; WBGene00011938 ; alh-13.

    Phylogenomic databases

    eggNOGi COG0014.
    GeneTreei ENSGT00500000044903.
    HOGENOMi HOG000246357.
    KOi K12657.
    OMAi LLPWVQS.
    OrthoDBi EOG7WX07N.

    Enzyme and pathway databases

    UniPathwayi UPA00098 ; UER00359 .
    UPA00098 ; UER00360 .

    Miscellaneous databases

    NextBioi 913848.
    PROi P54889.

    Family and domain databases

    Gene3Di 3.40.1160.10. 1 hit.
    3.40.309.10. 1 hit.
    3.40.605.10. 2 hits.
    HAMAPi MF_00412. ProA.
    MF_00456. ProB.
    InterProi IPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR016162. Ald_DH_N.
    IPR015590. Aldehyde_DH_dom.
    IPR001048. Asp/Glu/Uridylate_kinase.
    IPR000965. G-glutamylP_reductase.
    IPR020593. G-glutamylP_reductase_CS.
    IPR001057. Glu/AcGlu_kinase.
    IPR005715. Glu_5kinase/COase_Synthase.
    IPR019797. Glutamate_5-kinase_CS.
    IPR005766. P5_carboxy_syn.
    [Graphical view ]
    Pfami PF00696. AA_kinase. 1 hit.
    PF00171. Aldedh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036429. P5C_syn. 1 hit.
    PRINTSi PR00474. GLU5KINASE.
    SUPFAMi SSF53633. SSF53633. 1 hit.
    SSF53720. SSF53720. 1 hit.
    TIGRFAMsi TIGR01092. P5CS. 1 hit.
    TIGR00407. proA. 1 hit.
    TIGR01027. proB. 1 hit.
    PROSITEi PS00902. GLUTAMATE_5_KINASE. 1 hit.
    PS01223. PROA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.

    Entry informationi

    Entry nameiALH13_CAEEL
    AccessioniPrimary (citable) accession number: P54889
    Secondary accession number(s): Q9BI69
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: September 18, 2013
    Last modified: October 1, 2014
    This is version 107 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3