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P54889

- ALH13_CAEEL

UniProt

P54889 - ALH13_CAEEL

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Protein
Probable delta-1-pyrroline-5-carboxylate synthase
Gene
alh-13, T22H6.2
Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation
L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei128 – 1281Substrate By similarity
Binding sitei227 – 2271Substrate By similarity
Binding sitei242 – 2421Substrate; via amide nitrogen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi262 – 2632ATP By similarity
Nucleotide bindingi301 – 3077ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate 5-kinase activity Source: UniProtKB-EC
  3. glutamate-5-semialdehyde dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. L-proline biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Oxidoreductase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

ATP-binding, NADP, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.
UPA00098; UER00360.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable delta-1-pyrroline-5-carboxylate synthase
Short name:
P5CS
Including the following 2 domains:
Glutamate 5-kinase (EC:2.7.2.11)
Short name:
GK
Alternative name(s):
Gamma-glutamyl kinase
Gamma-glutamyl phosphate reductase (EC:1.2.1.41)
Short name:
GPR
Alternative name(s):
Aldehyde dehydrogenase family 13
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Gene namesi
Name:alh-13
ORF Names:T22H6.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome X

Organism-specific databases

WormBaseiT22H6.2a; CE47944; WBGene00011938; alh-13.
T22H6.2b; CE47964; WBGene00011938; alh-13.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: WormBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 802802Probable delta-1-pyrroline-5-carboxylate synthaseUniRule annotation
PRO_0000109771Add
BLAST

Proteomic databases

PaxDbiP54889.
PRIDEiP54889.

Interactioni

Protein-protein interaction databases

STRINGi6239.T22H6.2b.

Structurei

3D structure databases

ProteinModelPortaliP54889.
SMRiP54889. Positions 81-345, 356-782.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 354354Glutamate 5-kinaseUniRule annotation
Add
BLAST
Regioni355 – 802448Gamma-glutamyl phosphate reductaseUniRule annotation
Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glutamate 5-kinase family.
In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.

Phylogenomic databases

eggNOGiCOG0014.
GeneTreeiENSGT00500000044903.
HOGENOMiHOG000246357.
KOiK12657.
OMAiLLPWVQS.
OrthoDBiEOG7WX07N.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
3.40.309.10. 1 hit.
3.40.605.10. 2 hits.
HAMAPiMF_00412. ProA.
MF_00456. ProB.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR000965. G-glutamylP_reductase.
IPR020593. G-glutamylP_reductase_CS.
IPR001057. Glu/AcGlu_kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR005766. P5_carboxy_syn.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF00171. Aldedh. 1 hit.
[Graphical view]
PIRSFiPIRSF036429. P5C_syn. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01092. P5CS. 1 hit.
TIGR00407. proA. 1 hit.
TIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS01223. PROA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform b (identifier: P54889-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MFRATRCLRL PLRNSHINIL RPTQTELIKT RSSALAPYEK VSPITAVGAT    50
PVGVGNGNYC YSTKTRQKHP LINTRNDLKK AQRVVVKLGS AVITREDECG 100
LALGRLASIV EQVSELQQSG RQMLIVSSGA VAFGRQKLRQ ELVMSMSMRQ 150
TLRGPSGMTA DKRACAASGM PGLMSLYEQL FQQYGITVAQ VLLTKPDIDD 200
DQRRKNLQAT IESLLSLNII PIVNANDAVA PDPKLNMHIS DNDSLAARLS 250
AEIEAELLII LSNVNGVYTG PPDLEGSRLL YTYVPSENSG VTFGANSKFG 300
TGGMESKVTA CVNALNNGVT TVITNGLAQD AITDAVAGKK IGTMFCNTKG 350
YEGPPIEEVA EKCRDAGRQL AALSNKERGA MVRHLAALLV DKEKYIIEAN 400
QTDLANAKSA GLDPQLLNRL KMTPEKIQDL HAGLNTIADS AETLVGRVLK 450
KVKISEGLFL EQVTVPIGSL MVIFESRPDC LPQVASLAMA SGNALLLKGG 500
KEAEESNKAL HALVQEALGT HGFEMRDAVT LVRSREDVAD LLQLKDLIDL 550
IIPRGSSDLV RSMQEKSKGI PVLGHAEGVC HVYIDKDCDE QKAIQIVRDS 600
KCDYPSACNA AETILIHKDL ATAPFFDSLC SMFKAEGVKL HAGPKLAALL 650
KFAPPPAESM SFEYGSLECT LEVVDNVEEA VAHIIRYGSG HTESIITENT 700
NTAEHFLKHV DSACAFHNAS TRFADGYRFG LGAEVGISTG RIHARGPVGV 750
EGLLTTKWLL RGEGHLVEDF KNGKYSYLHE NLNPSEVYRA LDAAGELKKA 800
TA 802

Note: No experimental confirmation available.

Length:802
Mass (Da):86,694
Last modified:September 18, 2013 - v2
Checksum:i9342768572E138E9
GO
Isoform a (identifier: P54889-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     61-62: Missing.

Note: No experimental confirmation available.

Show »
Length:800
Mass (Da):86,444
Checksum:i287B2698EE2F5B88
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei61 – 622Missing in isoform a.
VSP_047890

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z50797 Genomic DNA. Translation: CAA90672.2.
Z50797 Genomic DNA. Translation: CAC35828.2.
PIRiT25140.
RefSeqiNP_510132.2. NM_077731.4. [P54889-1]
NP_510133.2. NM_077732.6. [P54889-2]
UniGeneiCel.17267.

Genome annotation databases

EnsemblMetazoaiT22H6.2b; T22H6.2b; WBGene00011938. [P54889-1]
GeneIDi181417.
KEGGicel:CELE_T22H6.2.
UCSCiT22H6.2b. c. elegans. [P54889-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z50797 Genomic DNA. Translation: CAA90672.2 .
Z50797 Genomic DNA. Translation: CAC35828.2 .
PIRi T25140.
RefSeqi NP_510132.2. NM_077731.4. [P54889-1 ]
NP_510133.2. NM_077732.6. [P54889-2 ]
UniGenei Cel.17267.

3D structure databases

ProteinModelPortali P54889.
SMRi P54889. Positions 81-345, 356-782.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 6239.T22H6.2b.

Proteomic databases

PaxDbi P54889.
PRIDEi P54889.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai T22H6.2b ; T22H6.2b ; WBGene00011938 . [P54889-1 ]
GeneIDi 181417.
KEGGi cel:CELE_T22H6.2.
UCSCi T22H6.2b. c. elegans. [P54889-1 ]

Organism-specific databases

CTDi 181417.
WormBasei T22H6.2a ; CE47944 ; WBGene00011938 ; alh-13.
T22H6.2b ; CE47964 ; WBGene00011938 ; alh-13.

Phylogenomic databases

eggNOGi COG0014.
GeneTreei ENSGT00500000044903.
HOGENOMi HOG000246357.
KOi K12657.
OMAi LLPWVQS.
OrthoDBi EOG7WX07N.

Enzyme and pathway databases

UniPathwayi UPA00098 ; UER00359 .
UPA00098 ; UER00360 .

Miscellaneous databases

NextBioi 913848.
PROi P54889.

Family and domain databases

Gene3Di 3.40.1160.10. 1 hit.
3.40.309.10. 1 hit.
3.40.605.10. 2 hits.
HAMAPi MF_00412. ProA.
MF_00456. ProB.
InterProi IPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR000965. G-glutamylP_reductase.
IPR020593. G-glutamylP_reductase_CS.
IPR001057. Glu/AcGlu_kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR005766. P5_carboxy_syn.
[Graphical view ]
Pfami PF00696. AA_kinase. 1 hit.
PF00171. Aldedh. 1 hit.
[Graphical view ]
PIRSFi PIRSF036429. P5C_syn. 1 hit.
PRINTSi PR00474. GLU5KINASE.
SUPFAMi SSF53633. SSF53633. 1 hit.
SSF53720. SSF53720. 1 hit.
TIGRFAMsi TIGR01092. P5CS. 1 hit.
TIGR00407. proA. 1 hit.
TIGR01027. proB. 1 hit.
PROSITEi PS00902. GLUTAMATE_5_KINASE. 1 hit.
PS01223. PROA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiALH13_CAEEL
AccessioniPrimary (citable) accession number: P54889
Secondary accession number(s): Q9BI69
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 18, 2013
Last modified: September 3, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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