Reviewed, UniProtKB/Swiss-Prot P54889 (P5CS_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable delta-1-pyrroline-5-carboxylate synthetase
      Short name=P5CS
Including the following 2 domains:
    1- Recommended name:
            Glutamate 5-kinase
                Short name=GK
              EC=2.7.2.11
        Alternative name(s):
            Gamma-glutamyl kinase
    2- Recommended name:
            Gamma-glutamyl phosphate reductase
                Short name=GPR
              EC=1.2.1.41
        Alternative name(s):
            Glutamate-5-semialdehyde dehydrogenase
            Glutamyl-gamma-semialdehyde dehydrogenase

Gene names

ORF Names:

T22H6.2

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	800 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. L-glutamate 5-semialdehyde + phosphate + NADP(+) = L-glutamyl 5-phosphate + NADPH.
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.
Sequence similarities	In the N-terminal section; belongs to the glutamate 5-kinase family. In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Ligand	NADP
Molecular function	Kinase Oxidoreductase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	glutamate 5-kinase activity Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 800	800	Probable delta-1-pyrroline-5-carboxylate synthetase		PRO_0000109771
Regions
	Region	1 – 352	352	Glutamate 5-kinase
	Region	353 – 800	448	Gamma-glutamyl phosphate reductase

Sequences

	Sequence		Length	Mass (Da)
	P54889-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 39223F4973368F00	FASTA	800	86,527
	10 20 30 40 50 60 MFRATRCLRL PLRNSHINIL RPTQTELIKT RSSALAPYEK VSPITAVGAT PVGVGNGNYC 70 80 90 100 110 120 TKTRQKHPLI NTRNDLKKAQ RVVVKLGSAV ITREDECGLA LGRLASIVEQ VSELQQSGRQ 130 140 150 160 170 180 MLIVSSGAVA FGRQKLRQEL VMSMSMRQTL RGPSGMTADK RACAASGMPG LMSLYEQLFQ 190 200 210 220 230 240 QYGITVAQVL LTKPDIDDDQ RRKNLQATIE SLLSLNIIPI VNANDAVAPD PKLNMHISDN 250 260 270 280 290 300 DSLAARLSAE IEAELLIILS NVNGVYTGPP DLEGSRLLYT YVPSENSGVT FGANSKFGTG 310 320 330 340 350 360 GMESKVTACV NALNNGVTTV ITNGLAQDAI TDAVAGKKIG TMFCNTKGYE GPPIEEVAEK 370 380 390 400 410 420 CRDAGRQLAA LSNKERGAMV RHLAALLVDK EKYIIEANQT DLANAKSAGL DPQLLNRLKM 430 440 450 460 470 480 TPEKIQDLHA GLNTIADSAE TLVGRVLKKV KISEGLFLEQ VTVPIGSLMV IFESRPDCLP 490 500 510 520 530 540 QVASLAMASG NALLLKGGKE AEESNKALHA LVQEALGTHG FEMRDAVTLV RSREDVADLL 550 560 570 580 590 600 QLKDLIDLII PRGSSDLVRS MQEKSKGIPV LGHAEGVWHV YIDKDCDEQK AIQIVRDSKC 610 620 630 640 650 660 DYPSACNAAE TILIHKDLAT APFFDSLCSM FKAEGVKLHA GPKLAALLKF APPPAESMSF 670 680 690 700 710 720 EYGSLECTLE VVDNVEEAVA HIIRYGSGHT ESIITENTNT AEHFLKHVDS ACAFHNASTR 730 740 750 760 770 780 FADGYRFGLG AEVGISTGRI HARGPVGVEG LLTTKWLLRG EGHLVEDFKN GKYSYLHENL 790 800 NPSEVYRALD AAGELKKATA

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z50797 Genomic DNA. Translation: CAA90672.1.
PIR	T25140.
RefSeq	NP_510133.1.
UniGene	Cel.17267
3D structure databases
HSSP	HSSP built from PDB template 1O20 based on UniProtKB Q9WYC9.
ModBase	Search...
Genome annotation databases
Ensembl	T22H6.2. Caenorhabditis elegans. [Contig view]
GeneID	181417.
Organism-specific databases
WormBase	WBGene00011938. T22H6.2.
WormPep	T22H6.2. CE03697. [WorfDB]
Gene expression databases
ArrayExpress	P54889.
Family and domain databases
InterPro	IPR016163. Ald_DHase_C. IPR016162. Ald_DHase_N. IPR001048. Asp/Glu/Uridylate_kinase. IPR000965. Gglut_pp_reduct. IPR001057. Glu_5kinase. IPR005766. P5_carboxy_syn. IPR005715. ProB. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit. G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF036429. P5C_syn. 1 hit.
PRINTS	PR00474. GLU5KINASE.
TIGRFAMs	TIGR01092. P5CS. 1 hit. TIGR00407. proA. 1 hit. TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	913848.

Entry information

Entry name

P5CS_CAEEL

Accession

Primary (citable) accession number: P54889

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 25, 2008
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project