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P54889 (ALH13_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable delta-1-pyrroline-5-carboxylate synthase

Short name=P5CS

Including the following 2 domains:

  1. Glutamate 5-kinase
    Short name=GK
    EC=2.7.2.11
    Alternative name(s):
    Gamma-glutamyl kinase
  2. Gamma-glutamyl phosphate reductase
    Short name=GPR
    EC=1.2.1.41
    Alternative name(s):
    Aldehyde dehydrogenase family 13
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
Gene names
Name:alh-13
ORF Names:T22H6.2
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length802 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP-Rule MF_00456

L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP-Rule MF_00456

Pathway

Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP-Rule MF_00456

Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.

Sequence similarities

In the N-terminal section; belongs to the glutamate 5-kinase family.

In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform b (identifier: P54889-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform a (identifier: P54889-2)

The sequence of this isoform differs from the canonical sequence as follows:
     61-62: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 802802Probable delta-1-pyrroline-5-carboxylate synthase HAMAP-Rule MF_00456
PRO_0000109771

Regions

Nucleotide binding262 – 2632ATP By similarity
Nucleotide binding301 – 3077ATP By similarity
Region1 – 354354Glutamate 5-kinase HAMAP-Rule MF_00456
Region355 – 802448Gamma-glutamyl phosphate reductase HAMAP-Rule MF_00456

Sites

Binding site1281Substrate By similarity
Binding site2271Substrate By similarity
Binding site2421Substrate; via amide nitrogen By similarity

Natural variations

Alternative sequence61 – 622Missing in isoform a.
VSP_047890

Sequences

Sequence LengthMass (Da)Tools
Isoform b [UniParc].

Last modified September 18, 2013. Version 2.
Checksum: 9342768572E138E9

FASTA80286,694
        10         20         30         40         50         60 
MFRATRCLRL PLRNSHINIL RPTQTELIKT RSSALAPYEK VSPITAVGAT PVGVGNGNYC 

        70         80         90        100        110        120 
YSTKTRQKHP LINTRNDLKK AQRVVVKLGS AVITREDECG LALGRLASIV EQVSELQQSG 

       130        140        150        160        170        180 
RQMLIVSSGA VAFGRQKLRQ ELVMSMSMRQ TLRGPSGMTA DKRACAASGM PGLMSLYEQL 

       190        200        210        220        230        240 
FQQYGITVAQ VLLTKPDIDD DQRRKNLQAT IESLLSLNII PIVNANDAVA PDPKLNMHIS 

       250        260        270        280        290        300 
DNDSLAARLS AEIEAELLII LSNVNGVYTG PPDLEGSRLL YTYVPSENSG VTFGANSKFG 

       310        320        330        340        350        360 
TGGMESKVTA CVNALNNGVT TVITNGLAQD AITDAVAGKK IGTMFCNTKG YEGPPIEEVA 

       370        380        390        400        410        420 
EKCRDAGRQL AALSNKERGA MVRHLAALLV DKEKYIIEAN QTDLANAKSA GLDPQLLNRL 

       430        440        450        460        470        480 
KMTPEKIQDL HAGLNTIADS AETLVGRVLK KVKISEGLFL EQVTVPIGSL MVIFESRPDC 

       490        500        510        520        530        540 
LPQVASLAMA SGNALLLKGG KEAEESNKAL HALVQEALGT HGFEMRDAVT LVRSREDVAD 

       550        560        570        580        590        600 
LLQLKDLIDL IIPRGSSDLV RSMQEKSKGI PVLGHAEGVC HVYIDKDCDE QKAIQIVRDS 

       610        620        630        640        650        660 
KCDYPSACNA AETILIHKDL ATAPFFDSLC SMFKAEGVKL HAGPKLAALL KFAPPPAESM 

       670        680        690        700        710        720 
SFEYGSLECT LEVVDNVEEA VAHIIRYGSG HTESIITENT NTAEHFLKHV DSACAFHNAS 

       730        740        750        760        770        780 
TRFADGYRFG LGAEVGISTG RIHARGPVGV EGLLTTKWLL RGEGHLVEDF KNGKYSYLHE 

       790        800 
NLNPSEVYRA LDAAGELKKA TA 

« Hide

Isoform a [UniParc].

Checksum: 287B2698EE2F5B88
Show »

FASTA80086,444

References

[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z50797 Genomic DNA. Translation: CAA90672.2.
Z50797 Genomic DNA. Translation: CAC35828.2.
PIRT25140.
RefSeqNP_510132.2. NM_077731.4.
NP_510133.2. NM_077732.6.
UniGeneCel.17267.

3D structure databases

ProteinModelPortalP54889.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING6239.T22H6.2b.

Proteomic databases

PaxDbP54889.
PRIDEP54889.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID181417.
KEGGcel:CELE_T22H6.2.
UCSCT22H6.2b. c. elegans. [P54889-1]

Organism-specific databases

CTD181417.
WormBaseT22H6.2a; CE47944; WBGene00011938; alh-13.
T22H6.2b; CE47964; WBGene00011938; alh-13.

Phylogenomic databases

eggNOGCOG0014.
HOGENOMHOG000246357.
KOK12657.
OrthoDBEOG7WX07N.

Enzyme and pathway databases

UniPathwayUPA00098; UER00359.
UPA00098; UER00360.

Family and domain databases

Gene3D3.40.1160.10. 1 hit.
3.40.309.10. 1 hit.
3.40.605.10. 2 hits.
HAMAPMF_00456. ProB.
InterProIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR000965. G-glutamylP_reductase.
IPR020593. G-glutamylP_reductase_CS.
IPR001057. Glu/AcGlu_kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR005766. P5_carboxy_syn.
[Graphical view]
PfamPF00696. AA_kinase. 1 hit.
PF00171. Aldedh. 1 hit.
[Graphical view]
PIRSFPIRSF036429. P5C_syn. 1 hit.
PRINTSPR00474. GLU5KINASE.
SUPFAMSSF53633. SSF53633. 1 hit.
SSF53720. SSF53720. 1 hit.
TIGRFAMsTIGR01092. P5CS. 1 hit.
TIGR00407. proA. 1 hit.
TIGR01027. proB. 1 hit.
PROSITEPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS01223. PROA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio913848.
PROP54889.

Entry information

Entry nameALH13_CAEEL
AccessionPrimary (citable) accession number: P54889
Secondary accession number(s): Q9BI69
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 18, 2013
Last modified: March 19, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase