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P54889

- ALH13_CAEEL

UniProt

P54889 - ALH13_CAEEL

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Protein

Probable delta-1-pyrroline-5-carboxylate synthase

Gene

alh-13

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.
L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei128 – 1281SubstrateBy similarity
Binding sitei227 – 2271SubstrateBy similarity
Binding sitei242 – 2421Substrate; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi262 – 2632ATPBy similarity
Nucleotide bindingi301 – 3077ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate 5-kinase activity Source: UniProtKB-EC
  3. glutamate-5-semialdehyde dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. L-proline biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Oxidoreductase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

ATP-binding, NADP, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_254462. Amino acid synthesis and interconversion (transamination).
UniPathwayiUPA00098; UER00359.
UPA00098; UER00360.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable delta-1-pyrroline-5-carboxylate synthase
Short name:
P5CS
Including the following 2 domains:
Glutamate 5-kinase (EC:2.7.2.11)
Short name:
GK
Alternative name(s):
Gamma-glutamyl kinase
Gamma-glutamyl phosphate reductase (EC:1.2.1.41)
Short name:
GPR
Alternative name(s):
Aldehyde dehydrogenase family 13
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Gene namesi
Name:alh-13
ORF Names:T22H6.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome X

Organism-specific databases

WormBaseiT22H6.2a; CE47944; WBGene00011938; alh-13.
T22H6.2b; CE47964; WBGene00011938; alh-13.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: WormBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 802802Probable delta-1-pyrroline-5-carboxylate synthasePRO_0000109771Add
BLAST

Proteomic databases

PaxDbiP54889.
PRIDEiP54889.

Interactioni

Protein-protein interaction databases

STRINGi6239.T22H6.2b.

Structurei

3D structure databases

ProteinModelPortaliP54889.
SMRiP54889. Positions 75-343, 356-782.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 354354Glutamate 5-kinaseAdd
BLAST
Regioni355 – 802448Gamma-glutamyl phosphate reductaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glutamate 5-kinase family.Curated
In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.Curated

Phylogenomic databases

eggNOGiCOG0014.
GeneTreeiENSGT00500000044903.
HOGENOMiHOG000246357.
InParanoidiP54889.
KOiK12657.
OMAiLLPWVQS.
OrthoDBiEOG7WX07N.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
3.40.309.10. 1 hit.
3.40.605.10. 2 hits.
HAMAPiMF_00412. ProA.
MF_00456. ProB.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR000965. G-glutamylP_reductase.
IPR020593. G-glutamylP_reductase_CS.
IPR001057. Glu/AcGlu_kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR005766. P5_carboxy_syn.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF00171. Aldedh. 1 hit.
[Graphical view]
PIRSFiPIRSF036429. P5C_syn. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01092. P5CS. 1 hit.
TIGR00407. proA. 1 hit.
TIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS01223. PROA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform b (identifier: P54889-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFRATRCLRL PLRNSHINIL RPTQTELIKT RSSALAPYEK VSPITAVGAT
60 70 80 90 100
PVGVGNGNYC YSTKTRQKHP LINTRNDLKK AQRVVVKLGS AVITREDECG
110 120 130 140 150
LALGRLASIV EQVSELQQSG RQMLIVSSGA VAFGRQKLRQ ELVMSMSMRQ
160 170 180 190 200
TLRGPSGMTA DKRACAASGM PGLMSLYEQL FQQYGITVAQ VLLTKPDIDD
210 220 230 240 250
DQRRKNLQAT IESLLSLNII PIVNANDAVA PDPKLNMHIS DNDSLAARLS
260 270 280 290 300
AEIEAELLII LSNVNGVYTG PPDLEGSRLL YTYVPSENSG VTFGANSKFG
310 320 330 340 350
TGGMESKVTA CVNALNNGVT TVITNGLAQD AITDAVAGKK IGTMFCNTKG
360 370 380 390 400
YEGPPIEEVA EKCRDAGRQL AALSNKERGA MVRHLAALLV DKEKYIIEAN
410 420 430 440 450
QTDLANAKSA GLDPQLLNRL KMTPEKIQDL HAGLNTIADS AETLVGRVLK
460 470 480 490 500
KVKISEGLFL EQVTVPIGSL MVIFESRPDC LPQVASLAMA SGNALLLKGG
510 520 530 540 550
KEAEESNKAL HALVQEALGT HGFEMRDAVT LVRSREDVAD LLQLKDLIDL
560 570 580 590 600
IIPRGSSDLV RSMQEKSKGI PVLGHAEGVC HVYIDKDCDE QKAIQIVRDS
610 620 630 640 650
KCDYPSACNA AETILIHKDL ATAPFFDSLC SMFKAEGVKL HAGPKLAALL
660 670 680 690 700
KFAPPPAESM SFEYGSLECT LEVVDNVEEA VAHIIRYGSG HTESIITENT
710 720 730 740 750
NTAEHFLKHV DSACAFHNAS TRFADGYRFG LGAEVGISTG RIHARGPVGV
760 770 780 790 800
EGLLTTKWLL RGEGHLVEDF KNGKYSYLHE NLNPSEVYRA LDAAGELKKA

TA

Note: No experimental confirmation available.

Length:802
Mass (Da):86,694
Last modified:September 18, 2013 - v2
Checksum:i9342768572E138E9
GO
Isoform a (identifier: P54889-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     61-62: Missing.

Note: No experimental confirmation available.

Show »
Length:800
Mass (Da):86,444
Checksum:i287B2698EE2F5B88
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei61 – 622Missing in isoform a. CuratedVSP_047890

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z50797 Genomic DNA. Translation: CAA90672.2.
Z50797 Genomic DNA. Translation: CAC35828.2.
PIRiT25140.
RefSeqiNP_510132.2. NM_077731.4. [P54889-1]
NP_510133.2. NM_077732.6. [P54889-2]
UniGeneiCel.17267.

Genome annotation databases

EnsemblMetazoaiT22H6.2b; T22H6.2b; WBGene00011938. [P54889-1]
GeneIDi181417.
KEGGicel:CELE_T22H6.2.
UCSCiT22H6.2b. c. elegans. [P54889-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z50797 Genomic DNA. Translation: CAA90672.2 .
Z50797 Genomic DNA. Translation: CAC35828.2 .
PIRi T25140.
RefSeqi NP_510132.2. NM_077731.4. [P54889-1 ]
NP_510133.2. NM_077732.6. [P54889-2 ]
UniGenei Cel.17267.

3D structure databases

ProteinModelPortali P54889.
SMRi P54889. Positions 75-343, 356-782.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 6239.T22H6.2b.

Proteomic databases

PaxDbi P54889.
PRIDEi P54889.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai T22H6.2b ; T22H6.2b ; WBGene00011938 . [P54889-1 ]
GeneIDi 181417.
KEGGi cel:CELE_T22H6.2.
UCSCi T22H6.2b. c. elegans. [P54889-1 ]

Organism-specific databases

CTDi 181417.
WormBasei T22H6.2a ; CE47944 ; WBGene00011938 ; alh-13.
T22H6.2b ; CE47964 ; WBGene00011938 ; alh-13.

Phylogenomic databases

eggNOGi COG0014.
GeneTreei ENSGT00500000044903.
HOGENOMi HOG000246357.
InParanoidi P54889.
KOi K12657.
OMAi LLPWVQS.
OrthoDBi EOG7WX07N.

Enzyme and pathway databases

UniPathwayi UPA00098 ; UER00359 .
UPA00098 ; UER00360 .
Reactomei REACT_254462. Amino acid synthesis and interconversion (transamination).

Miscellaneous databases

NextBioi 913848.
PROi P54889.

Family and domain databases

Gene3Di 3.40.1160.10. 1 hit.
3.40.309.10. 1 hit.
3.40.605.10. 2 hits.
HAMAPi MF_00412. ProA.
MF_00456. ProB.
InterProi IPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR000965. G-glutamylP_reductase.
IPR020593. G-glutamylP_reductase_CS.
IPR001057. Glu/AcGlu_kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR005766. P5_carboxy_syn.
[Graphical view ]
Pfami PF00696. AA_kinase. 1 hit.
PF00171. Aldedh. 1 hit.
[Graphical view ]
PIRSFi PIRSF036429. P5C_syn. 1 hit.
PRINTSi PR00474. GLU5KINASE.
SUPFAMi SSF53633. SSF53633. 1 hit.
SSF53720. SSF53720. 1 hit.
TIGRFAMsi TIGR01092. P5CS. 1 hit.
TIGR00407. proA. 1 hit.
TIGR01027. proB. 1 hit.
PROSITEi PS00902. GLUTAMATE_5_KINASE. 1 hit.
PS01223. PROA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiALH13_CAEEL
AccessioniPrimary (citable) accession number: P54889
Secondary accession number(s): Q9BI69
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 18, 2013
Last modified: November 26, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3