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P54889 (ALH13_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable delta-1-pyrroline-5-carboxylate synthase
Short name=P5CS

Including the following 2 domains:

  1. Glutamate 5-kinase
    Short name=GK
    EC=2.7.2.11
    Alternative name(s):
    Gamma-glutamyl kinase
  2. Gamma-glutamyl phosphate reductase
    Short name=GPR
    EC=1.2.1.41
    Alternative name(s):
    Aldehyde dehydrogenase family 13
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
Gene names
Name:alh-13
ORF Names:T22H6.2
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length800 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.

L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH.

Pathway

Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2.

Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.

Sequence similarities

In the N-terminal section; belongs to the glutamate 5-kinase family.

In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 800800Probable delta-1-pyrroline-5-carboxylate synthase
PRO_0000109771

Regions

Nucleotide binding260 – 2612ATP By similarity
Nucleotide binding299 – 3057ATP By similarity
Region1 – 352352Glutamate 5-kinase
Region353 – 800448Gamma-glutamyl phosphate reductase

Sites

Binding site1261Substrate By similarity
Binding site2251Substrate By similarity
Binding site2401Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
P54889 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 39223F4973368F00

FASTA80086,527
        10         20         30         40         50         60 
MFRATRCLRL PLRNSHINIL RPTQTELIKT RSSALAPYEK VSPITAVGAT PVGVGNGNYC 

        70         80         90        100        110        120 
TKTRQKHPLI NTRNDLKKAQ RVVVKLGSAV ITREDECGLA LGRLASIVEQ VSELQQSGRQ 

       130        140        150        160        170        180 
MLIVSSGAVA FGRQKLRQEL VMSMSMRQTL RGPSGMTADK RACAASGMPG LMSLYEQLFQ 

       190        200        210        220        230        240 
QYGITVAQVL LTKPDIDDDQ RRKNLQATIE SLLSLNIIPI VNANDAVAPD PKLNMHISDN 

       250        260        270        280        290        300 
DSLAARLSAE IEAELLIILS NVNGVYTGPP DLEGSRLLYT YVPSENSGVT FGANSKFGTG 

       310        320        330        340        350        360 
GMESKVTACV NALNNGVTTV ITNGLAQDAI TDAVAGKKIG TMFCNTKGYE GPPIEEVAEK 

       370        380        390        400        410        420 
CRDAGRQLAA LSNKERGAMV RHLAALLVDK EKYIIEANQT DLANAKSAGL DPQLLNRLKM 

       430        440        450        460        470        480 
TPEKIQDLHA GLNTIADSAE TLVGRVLKKV KISEGLFLEQ VTVPIGSLMV IFESRPDCLP 

       490        500        510        520        530        540 
QVASLAMASG NALLLKGGKE AEESNKALHA LVQEALGTHG FEMRDAVTLV RSREDVADLL 

       550        560        570        580        590        600 
QLKDLIDLII PRGSSDLVRS MQEKSKGIPV LGHAEGVWHV YIDKDCDEQK AIQIVRDSKC 

       610        620        630        640        650        660 
DYPSACNAAE TILIHKDLAT APFFDSLCSM FKAEGVKLHA GPKLAALLKF APPPAESMSF 

       670        680        690        700        710        720 
EYGSLECTLE VVDNVEEAVA HIIRYGSGHT ESIITENTNT AEHFLKHVDS ACAFHNASTR 

       730        740        750        760        770        780 
FADGYRFGLG AEVGISTGRI HARGPVGVEG LLTTKWLLRG EGHLVEDFKN GKYSYLHENL 

       790        800 
NPSEVYRALD AAGELKKATA

« Hide

References

[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Z50797 Genomic DNA. Translation: CAA90672.1.
PIRT25140.
RefSeqNP_510133.2. NM_077732.6.

3D structure databases

ProteinModelPortalP54889.
SMRP54889. Positions 79-343, 354-780.
ModBaseSearch...

Proteomic databases

PaxDbP54889.
PRIDEP54889.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaT22H6.2a; T22H6.2a; T22H6.2.
GeneID181417.
KEGGcel:CELE_T22H6.2.
UCSCT22H6.2b. c. elegans.

Organism-specific databases

CTD181417.
WormBaseT22H6.2a; CE03697; WBGene00011938; alh-13.

Phylogenomic databases

eggNOGCOG0014.
GeneTreeENSGT00500000044903.
HOGENOMHOG000246357.
KOK12657.

Enzyme and pathway databases

UniPathwayUPA00098; UER00359.
UPA00098; UER00360.

Gene expression databases

ArrayExpressP54889.

Family and domain databases

Gene3D3.40.1160.10. 1 hit.
3.40.309.10. 1 hit.
3.40.605.10. 2 hits.
InterProIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR000965. G-glutamylP_reductase.
IPR020593. G-glutamylP_reductase_CS.
IPR001057. Glu/AcGlu_kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR005766. P5_carboxy_syn.
[Graphical view]
PfamPF00696. AA_kinase. 1 hit.
PF00171. Aldedh. 1 hit.
[Graphical view]
PIRSFPIRSF036429. P5C_syn. 1 hit.
PRINTSPR00474. GLU5KINASE.
SUPFAMSSF53633. Aa_kinase. 1 hit.
SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMsTIGR01092. P5CS. 1 hit.
TIGR00407. proA. 1 hit.
TIGR01027. proB. 1 hit.
PROSITEPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS01223. PROA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio913848.

Entry information

Entry nameALH13_CAEEL
AccessionPrimary (citable) accession number: P54889
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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