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Protein

Delta-1-pyrroline-5-carboxylate synthase B

Gene

P5CSB

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants.

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.
L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH.

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 and 2 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Delta-1-pyrroline-5-carboxylate synthase (AXX17_At3g50230), Delta-1-pyrroline-5-carboxylate synthase (AXX17_At2g36770), Delta-1-pyrroline-5-carboxylate synthase (P5CS1), Delta-1-pyrroline-5-carboxylate synthase (At2g39800), Delta-1-pyrroline-5-carboxylate synthase B (P5CSB), Delta-1-pyrroline-5-carboxylate synthase A (P5CSA)
  2. Delta-1-pyrroline-5-carboxylate synthase (AXX17_At3g50230), Delta-1-pyrroline-5-carboxylate synthase (AXX17_At2g36770), Delta-1-pyrroline-5-carboxylate synthase (P5CS1), Delta-1-pyrroline-5-carboxylate synthase (At2g39800), Delta-1-pyrroline-5-carboxylate synthase B (P5CSB), Delta-1-pyrroline-5-carboxylate synthase A (P5CSA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei60SubstrateBy similarity1
Binding sitei157SubstrateBy similarity1
Binding sitei176Substrate; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi196 – 197ATPBy similarity2
Nucleotide bindingi236 – 242ATPBy similarity7

GO - Molecular functioni

GO - Biological processi

  • embryo development ending in seed dormancy Source: TAIR
  • L-proline biosynthetic process Source: UniProtKB-UniPathway
  • pollen development Source: TAIR
  • proline biosynthetic process Source: TAIR

Keywordsi

Molecular functionKinase, Multifunctional enzyme, Oxidoreductase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandATP-binding, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G55610-MONOMER
ReactomeiR-ATH-70614 Amino acid synthesis and interconversion (transamination)
UniPathwayiUPA00098; UER00359
UPA00098; UER00360

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-1-pyrroline-5-carboxylate synthase B
Short name:
P5CS B
Including the following 2 domains:
Glutamate 5-kinase (EC:2.7.2.11)
Short name:
GK
Alternative name(s):
Gamma-glutamyl kinase
Gamma-glutamyl phosphate reductase (EC:1.2.1.41)
Short name:
GPR
Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Gene namesi
Name:P5CSB
Synonyms:P5CS2
Ordered Locus Names:At3g55610
ORF Names:F1I16_20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G55610
TAIRilocus:2078911 AT3G55610

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001097731 – 726Delta-1-pyrroline-5-carboxylate synthase BAdd BLAST726

Proteomic databases

PaxDbiP54888
PRIDEiP54888

PTM databases

iPTMnetiP54888

Expressioni

Gene expression databases

ExpressionAtlasiP54888 baseline and differential
GenevisibleiP54888 AT

Interactioni

Protein-protein interaction databases

BioGridi10043, 2 interactors
IntActiP54888, 2 interactors
STRINGi3702.AT3G55610.1

Structurei

3D structure databases

ProteinModelPortaliP54888
SMRiP54888
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 296Glutamate 5-kinaseAdd BLAST296
Regioni297 – 717Gamma-glutamyl phosphate reductaseAdd BLAST421

Sequence similaritiesi

In the N-terminal section; belongs to the glutamate 5-kinase family.Curated
In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.Curated

Phylogenomic databases

eggNOGiKOG1154 Eukaryota
KOG4165 Eukaryota
COG0014 LUCA
COG0263 LUCA
HOGENOMiHOG000246357
InParanoidiP54888
KOiK12657
OMAiETKSFHH
OrthoDBiEOG093605RS
PhylomeDBiP54888

Family and domain databases

CDDicd07079 ALDH_F18-19_ProA-GPR, 1 hit
Gene3Di3.40.1160.10, 1 hit
3.40.309.10, 1 hit
3.40.605.10, 2 hits
HAMAPiMF_00412 ProA, 1 hit
MF_00456 ProB, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR016161 Ald_DH/histidinol_DH
IPR016163 Ald_DH_C
IPR016162 Ald_DH_N
IPR001048 Asp/Glu/Uridylate_kinase
IPR020593 G-glutamylP_reductase_CS
IPR001057 Glu/AcGlu_kinase
IPR005715 Glu_5kinase/COase_Synthase
IPR019797 Glutamate_5-kinase_CS
IPR000965 GPR_dom
IPR005766 P5_carboxy_syn
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PIRSFiPIRSF036429 P5C_syn, 1 hit
PRINTSiPR00474 GLU5KINASE
SUPFAMiSSF53633 SSF53633, 1 hit
SSF53720 SSF53720, 1 hit
TIGRFAMsiTIGR01092 P5CS, 1 hit
TIGR00407 proA, 1 hit
TIGR01027 proB, 1 hit
PROSITEiView protein in PROSITE
PS00902 GLUTAMATE_5_KINASE, 1 hit
PS01223 PROA, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P54888-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTEIDRSRAF AKDVKRIVVK VGTAVVTGKG GRLALGRLGA ICEQLAELNS
60 70 80 90 100
DGFEVILVSS GAVGLGRQRL RYRQLVNSSF ADLQKPQMEL DGKACAGVGQ
110 120 130 140 150
SSLMAYYETM FDQLDVTVAQ MLVTDSSFRD KDFRKQLSET VKAMLRMRVI
160 170 180 190 200
PVFNENDAIS TRRAPYKDST GIFWDNDSLA ALLSLELKAD LLILLSDVEG
210 220 230 240 250
LYTGPPSDST SKLIHTFIKE KHQDEITFGE KSKLGRGGMT AKVKAAVNAA
260 270 280 290 300
YGGVPVIITS GYAAENISKV LRGLRVGTLF HQDAHLWAPV VDTTSRDMAV
310 320 330 340 350
AARESSRKLQ ALSSEDRKQI LHDIANALEV NEKTIKAEND LDVAAAQEAG
360 370 380 390 400
YEESLVARLV MKPGKISSLA ASVRQLAEME DPIGRVLKKT QVADDLILEK
410 420 430 440 450
TSSPIGVLLI VFESRPDALV QIASLAIRSG NGLLLKGGKE ARRSNAILHK
460 470 480 490 500
VITDAIPETV GGKLIGLVTS REEIPDLLKL DDVIDLVIPR GSNKLVSQIK
510 520 530 540 550
NSTKIPVLGH ADGICHVYVD KSGKLDMAKR IVSDAKLDYP AACNAMETLL
560 570 580 590 600
VHKDLEQNGF LDDLIYVLQT KGVTLYGGPR ASAKLNIPET KSFHHEYSSK
610 620 630 640 650
ACTVEIVEDV YGAIDHIHQH GSAHTDCIVT EDSEVAEIFL RQVDSAAVFH
660 670 680 690 700
NASTRFSDGF RFGLGAEVGI STSRIHARGP VGVEGLLTTR WIMRGKGQVV
710 720
DGDNGIVYTH KDLPVLQRTE AVENGI
Length:726
Mass (Da):78,871
Last modified:October 1, 1996 - v1
Checksum:iE01446A6659021FF
GO

Sequence cautioni

The sequence CAB81586 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X86778 Genomic DNA Translation: CAA60447.1
Y09355 mRNA Translation: CAA70527.1
AL161667 Genomic DNA Translation: CAB81586.1 Sequence problems.
CP002686 Genomic DNA Translation: AEE79408.1
AY091766 mRNA Translation: AAM10314.1
PIRiT47700
T50682 T50684
RefSeqiNP_191120.2, NM_115419.5 [P54888-1]
UniGeneiAt.25196

Genome annotation databases

EnsemblPlantsiAT3G55610.1; AT3G55610.1; AT3G55610 [P54888-1]
GeneIDi824727
GrameneiAT3G55610.1; AT3G55610.1; AT3G55610 [P54888-1]
KEGGiath:AT3G55610

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiP5CS2_ARATH
AccessioniPrimary (citable) accession number: P54888
Secondary accession number(s): Q9M061
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 25, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health