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Protein

Gamma-glutamyl phosphate reductase

Gene

PRO2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.

Miscellaneous

Present with 6920 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH.

Pathwayi: L-proline biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (PRO1)
  2. Gamma-glutamyl phosphate reductase (PRO2)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • glutamate-5-semialdehyde dehydrogenase activity Source: SGD
  • NADP binding Source: InterPro

GO - Biological processi

  • L-proline biosynthetic process Source: UniProtKB-UniPathway
  • proline biosynthetic process Source: SGD

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandNADP

Enzyme and pathway databases

BioCyciYEAST:YOR323C-MONOMER
ReactomeiR-SCE-70614 Amino acid synthesis and interconversion (transamination)
UniPathwayiUPA00098; UER00360

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-glutamyl phosphate reductase (EC:1.2.1.41)
Short name:
GPR
Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Short name:
GSA dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Gene namesi
Name:PRO2
Ordered Locus Names:YOR323C
ORF Names:O6155
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR323C
SGDiS000005850 PRO2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001898242 – 456Gamma-glutamyl phosphate reductaseAdd BLAST455

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP54885
PaxDbiP54885
PRIDEiP54885

PTM databases

iPTMnetiP54885

Interactioni

Protein-protein interaction databases

BioGridi34709, 31 interactors
DIPiDIP-6586N
IntActiP54885, 10 interactors
MINTiP54885
STRINGi4932.YOR323C

Structurei

Secondary structure

1456
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 18Combined sources16
Helixi23 – 39Combined sources17
Helixi41 – 57Combined sources17
Helixi62 – 68Combined sources7
Helixi75 – 88Combined sources14
Beta strandi92 – 94Combined sources3
Beta strandi96 – 103Combined sources8
Beta strandi106 – 114Combined sources9
Beta strandi117 – 124Combined sources8
Helixi127 – 139Combined sources13
Beta strandi142 – 146Combined sources5
Helixi149 – 151Combined sources3
Helixi152 – 173Combined sources22
Beta strandi179 – 182Combined sources4
Helixi190 – 194Combined sources5
Turni196 – 198Combined sources3
Beta strandi201 – 206Combined sources6
Helixi208 – 216Combined sources9
Beta strandi230 – 234Combined sources5
Helixi240 – 249Combined sources10
Beta strandi262 – 266Combined sources5
Helixi273 – 284Combined sources12
Beta strandi288 – 290Combined sources3
Helixi292 – 305Combined sources14
Helixi310 – 313Combined sources4
Beta strandi335 – 338Combined sources4
Helixi342 – 349Combined sources8
Beta strandi357 – 361Combined sources5
Helixi365 – 374Combined sources10
Beta strandi378 – 384Combined sources7
Helixi386 – 388Combined sources3
Helixi416 – 418Combined sources3
Beta strandi419 – 426Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VLUX-ray2.29A/B1-456[»]
ProteinModelPortaliP54885
SMRiP54885
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP54885

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00500000044903
HOGENOMiHOG000246356
InParanoidiP54885
KOiK00147
OMAiHGSHHTD
OrthoDBiEOG092C3AH4

Family and domain databases

CDDicd07079 ALDH_F18-19_ProA-GPR, 1 hit
Gene3Di3.40.309.10, 1 hit
3.40.605.10, 3 hits
HAMAPiMF_00412 ProA, 1 hit
InterProiView protein in InterPro
IPR016161 Ald_DH/histidinol_DH
IPR016163 Ald_DH_C
IPR016162 Ald_DH_N
IPR015590 Aldehyde_DH_dom
IPR020593 G-glutamylP_reductase_CS
IPR012134 Glu-5-SA_DH
IPR000965 GPR_dom
PfamiView protein in Pfam
PF00171 Aldedh, 1 hit
PIRSFiPIRSF000151 GPR, 1 hit
SUPFAMiSSF53720 SSF53720, 1 hit
TIGRFAMsiTIGR00407 proA, 1 hit
PROSITEiView protein in PROSITE
PS01223 PROA, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P54885-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSQQIAKN ARKAGNILKT ISNEGRSDIL YKIHDALKAN AHAIEEANKI
60 70 80 90 100
DLAVAKETGL ADSLLKRLDL FKGDKFEVML QGIKDVAELE DPVGKVKMAR
110 120 130 140 150
ELDDGLTLYQ VTAPVGVLLV IFESRPEVIA NITALSIKSG NAAILKGGKE
160 170 180 190 200
SVNTFREMAK IVNDTIAQFQ SETGVPVGSV QLIETRQDVS DLLDQDEYID
210 220 230 240 250
LVVPRGSNAL VRKIKDTTKI PVLGHADGIC SIYLDEDADL IKAKRISLDA
260 270 280 290 300
KTNYPAGCNA METLLINPKF SKWWEVLENL TLEGGVTIHA TKDLKTAYFD
310 320 330 340 350
KLNELGKLTE AIQCKTVDAD EEQDFDKEFL SLDLAAKFVT STESAIQHIN
360 370 380 390 400
THSSRHTDAI VTENKANAEK FMKGVDSSGV YWNASTRFAD GFRYGFGAEV
410 420 430 440 450
GISTSKIHAR GPVGLDGLVS YQYQIRGDGQ VASDYLGAGG NKAFVHKDLD

IKTVTL
Length:456
Mass (Da):49,740
Last modified:October 1, 1996 - v1
Checksum:i7EDEC40C9B3DB07D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43565 Genomic DNA Translation: AAA86261.1
X90565 Genomic DNA Translation: CAA62179.1
Z75231 Genomic DNA Translation: CAA99643.1
AY692984 Genomic DNA Translation: AAT93003.1
BK006948 Genomic DNA Translation: DAA11087.1
PIRiS58334
RefSeqiNP_014968.1, NM_001183743.1

Genome annotation databases

EnsemblFungiiYOR323C; YOR323C; YOR323C
GeneIDi854501
KEGGisce:YOR323C

Similar proteinsi

Entry informationi

Entry nameiPROA_YEAST
AccessioniPrimary (citable) accession number: P54885
Secondary accession number(s): D6W321
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 162 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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