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Protein

Gamma-glutamyl phosphate reductase

Gene

PRO2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.

Catalytic activityi

L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH.

Pathwayi: L-proline biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (PRO1)
  2. Gamma-glutamyl phosphate reductase (PRO2)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • glutamate-5-semialdehyde dehydrogenase activity Source: SGD
  • NADP binding Source: InterPro

GO - Biological processi

  • L-proline biosynthetic process Source: UniProtKB-UniPathway
  • proline biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciYEAST:YOR323C-MONOMER.
ReactomeiR-SCE-70614. Amino acid synthesis and interconversion (transamination).
UniPathwayiUPA00098; UER00360.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-glutamyl phosphate reductase (EC:1.2.1.41)
Short name:
GPR
Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Short name:
GSA dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Gene namesi
Name:PRO2
Ordered Locus Names:YOR323C
ORF Names:O6155
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR323C.
SGDiS000005850. PRO2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001898242 – 456Gamma-glutamyl phosphate reductaseAdd BLAST455

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP54885.
PRIDEiP54885.

Interactioni

Protein-protein interaction databases

BioGridi34709. 30 interactors.
DIPiDIP-6586N.
IntActiP54885. 10 interactors.
MINTiMINT-615046.

Structurei

Secondary structure

1456
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 18Combined sources16
Helixi23 – 39Combined sources17
Helixi41 – 57Combined sources17
Helixi62 – 68Combined sources7
Helixi75 – 88Combined sources14
Beta strandi92 – 94Combined sources3
Beta strandi96 – 103Combined sources8
Beta strandi106 – 114Combined sources9
Beta strandi117 – 124Combined sources8
Helixi127 – 139Combined sources13
Beta strandi142 – 146Combined sources5
Helixi149 – 151Combined sources3
Helixi152 – 173Combined sources22
Beta strandi179 – 182Combined sources4
Helixi190 – 194Combined sources5
Turni196 – 198Combined sources3
Beta strandi201 – 206Combined sources6
Helixi208 – 216Combined sources9
Beta strandi230 – 234Combined sources5
Helixi240 – 249Combined sources10
Beta strandi262 – 266Combined sources5
Helixi273 – 284Combined sources12
Beta strandi288 – 290Combined sources3
Helixi292 – 305Combined sources14
Helixi310 – 313Combined sources4
Beta strandi335 – 338Combined sources4
Helixi342 – 349Combined sources8
Beta strandi357 – 361Combined sources5
Helixi365 – 374Combined sources10
Beta strandi378 – 384Combined sources7
Helixi386 – 388Combined sources3
Helixi416 – 418Combined sources3
Beta strandi419 – 426Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VLUX-ray2.29A/B1-456[»]
ProteinModelPortaliP54885.
SMRiP54885.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP54885.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00500000044903.
HOGENOMiHOG000246356.
InParanoidiP54885.
KOiK00147.
OMAiCNAIETL.
OrthoDBiEOG092C3AH4.

Family and domain databases

CDDicd07079. ALDH_F18-19_ProA-GPR. 1 hit.
Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 2 hits.
HAMAPiMF_00412. ProA. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR020593. G-glutamylP_reductase_CS.
IPR012134. Glu-5-SA_DH.
IPR000965. GPR_dom.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
PIRSFiPIRSF000151. GPR. 1 hit.
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR00407. proA. 1 hit.
PROSITEiPS01223. PROA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P54885-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSQQIAKN ARKAGNILKT ISNEGRSDIL YKIHDALKAN AHAIEEANKI
60 70 80 90 100
DLAVAKETGL ADSLLKRLDL FKGDKFEVML QGIKDVAELE DPVGKVKMAR
110 120 130 140 150
ELDDGLTLYQ VTAPVGVLLV IFESRPEVIA NITALSIKSG NAAILKGGKE
160 170 180 190 200
SVNTFREMAK IVNDTIAQFQ SETGVPVGSV QLIETRQDVS DLLDQDEYID
210 220 230 240 250
LVVPRGSNAL VRKIKDTTKI PVLGHADGIC SIYLDEDADL IKAKRISLDA
260 270 280 290 300
KTNYPAGCNA METLLINPKF SKWWEVLENL TLEGGVTIHA TKDLKTAYFD
310 320 330 340 350
KLNELGKLTE AIQCKTVDAD EEQDFDKEFL SLDLAAKFVT STESAIQHIN
360 370 380 390 400
THSSRHTDAI VTENKANAEK FMKGVDSSGV YWNASTRFAD GFRYGFGAEV
410 420 430 440 450
GISTSKIHAR GPVGLDGLVS YQYQIRGDGQ VASDYLGAGG NKAFVHKDLD

IKTVTL
Length:456
Mass (Da):49,740
Last modified:October 1, 1996 - v1
Checksum:i7EDEC40C9B3DB07D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43565 Genomic DNA. Translation: AAA86261.1.
X90565 Genomic DNA. Translation: CAA62179.1.
Z75231 Genomic DNA. Translation: CAA99643.1.
AY692984 Genomic DNA. Translation: AAT93003.1.
BK006948 Genomic DNA. Translation: DAA11087.1.
PIRiS58334.
RefSeqiNP_014968.1. NM_001183743.1.

Genome annotation databases

EnsemblFungiiYOR323C; YOR323C; YOR323C.
GeneIDi854501.
KEGGisce:YOR323C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43565 Genomic DNA. Translation: AAA86261.1.
X90565 Genomic DNA. Translation: CAA62179.1.
Z75231 Genomic DNA. Translation: CAA99643.1.
AY692984 Genomic DNA. Translation: AAT93003.1.
BK006948 Genomic DNA. Translation: DAA11087.1.
PIRiS58334.
RefSeqiNP_014968.1. NM_001183743.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VLUX-ray2.29A/B1-456[»]
ProteinModelPortaliP54885.
SMRiP54885.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34709. 30 interactors.
DIPiDIP-6586N.
IntActiP54885. 10 interactors.
MINTiMINT-615046.

Proteomic databases

MaxQBiP54885.
PRIDEiP54885.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR323C; YOR323C; YOR323C.
GeneIDi854501.
KEGGisce:YOR323C.

Organism-specific databases

EuPathDBiFungiDB:YOR323C.
SGDiS000005850. PRO2.

Phylogenomic databases

GeneTreeiENSGT00500000044903.
HOGENOMiHOG000246356.
InParanoidiP54885.
KOiK00147.
OMAiCNAIETL.
OrthoDBiEOG092C3AH4.

Enzyme and pathway databases

UniPathwayiUPA00098; UER00360.
BioCyciYEAST:YOR323C-MONOMER.
ReactomeiR-SCE-70614. Amino acid synthesis and interconversion (transamination).

Miscellaneous databases

EvolutionaryTraceiP54885.
PROiP54885.

Family and domain databases

CDDicd07079. ALDH_F18-19_ProA-GPR. 1 hit.
Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 2 hits.
HAMAPiMF_00412. ProA. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR020593. G-glutamylP_reductase_CS.
IPR012134. Glu-5-SA_DH.
IPR000965. GPR_dom.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
PIRSFiPIRSF000151. GPR. 1 hit.
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR00407. proA. 1 hit.
PROSITEiPS01223. PROA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPROA_YEAST
AccessioniPrimary (citable) accession number: P54885
Secondary accession number(s): D6W321
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 152 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6920 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.