Reviewed, UniProtKB/Swiss-Prot P54875 (DNLI_METTF)

Names and origin

Protein names

Recommended name:
    DNA ligase
    EC=6.5.1.1
Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:

lig

Organism

Methanobacterium thermoformicicum

Taxonomic identifier

145262 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	557 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This protein seals, during DNA replication, DNA recombination and DNA repair, nicks in double-stranded DNA By similarity.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 557	557	DNA ligase		PRO_0000059608
Sites
	Active site	251	1	N6-AMP-lysine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P54875-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: C328FE239AD1CBAA	FASTA	557	62,822
	10 20 30 40 50 60 MKELLYMELA EVYHRLESTT KRLEKTEILA ELLRSVDKEL LPDVTILMLG RVFPIWSEEE 70 80 90 100 110 120 LGVGIKLLMK AISLVVGVSM DEIEDEIREQ GDIGLASEKL FSRKTQTTFF SQPLTVEFVY 130 140 150 160 170 180 SRLKALASAS GDRAQSKKIN ILVEVLSQAK PLEARYITRT VLEELRVGVA EGIIRDAIAR 190 200 210 220 230 240 AFEVDPALVE RAHMLTNDLG MVAAVAREEG EPGLGRLNLE PGRPVKPMLA QLASSIESAI 250 260 270 280 290 300 TELGRAFCET KYDGIRVQIH RCGDEVSIFT RRLENITAAV PEILEGVEEA LPADDYIVEG 310 320 330 340 350 360 EIIVTMDGRP ASFQYILQRV RRKYDVDRLT REVPLSLFLF DVLYHRGPLI DEPLWHRREV 370 380 390 400 410 420 LESILSEIPG RVEASRMVDV GPDNLDDALW LFKESIREGH EGIMIKDTEA PYIPGIRGKK 430 440 450 460 470 480 MLKFKAEPET LDLIVVGGTY GRGKRAHLVG SYLLAVRDEK SGELKTIAHV ATGLDDQTLQ 490 500 510 520 530 540 DLSERMENLK VERRGRKIRV KPEIILEVAY SEIVRSPEYE SGYSLRFPVV KRIRDDLSPD 550 DIDTLGRVIS LFKQGFS

References

[1]	"Growth- and substrate-dependent transcription of the formate dehydrogenase (fdhCAB) operon in Methanobacterium thermoformicicum Z-245." Noelling J., Reeve J.N. J. Bacteriol. 179:899-908(1997) [PubMed: 9006048] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 3720 / Z-245.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U52681 Genomic DNA. Translation: AAC44823.1.
3D structure databases
ModBase	Search...
Family and domain databases
HAMAP	MF_00407. [Tree]
InterPro	IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR012308. DNA_ligase_A_N. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_METTF

Accession

Primary (citable) accession number: P54875

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 25, 2008
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)