Reviewed, UniProtKB/Swiss-Prot P54874 (HMCS_SCHPO)

Names and origin

Protein names

Recommended name:
    Hydroxymethylglutaryl-CoA synthase
      Short name=HMG-CoA synthase
    EC=2.3.3.10
Alternative name(s):
    3-hydroxy-3-methylglutaryl coenzyme A synthase

Gene names

Name:	hcs1
Synonyms:	hcs
ORF Names:	SPAC4F8.14c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase By similarity.
Catalytic activity	Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.
Sequence similarities	Belongs to the HMG-CoA synthase family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Molecular function	Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	acetyl-CoA metabolic process Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe ergosterol biosynthetic process Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe farnesyl diphosphate biosynthetic process, mevalonate pathway Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_SPombe endoplasmic reticulum Inferred by curator. Source: GeneDB_SPombe nucleus Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	hydroxymethylglutaryl-CoA synthase activity Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	Hydroxymethylglutaryl-CoA synthase		PRO_0000213757
Sites
	Active site	118	1	Potential
Amino acid modifications
	Modified residue	398	1	Phosphothreonine Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P54874-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 919BDDBD9207B886	FASTA	447	49,239
	10 20 30 40 50 60 MSFDRKDIGI KGLVLYTPNQ YVEQAALEAH DGVSTGKYTI GLGLTKMAFV DDREDIYSFG 70 80 90 100 110 120 LTALSQLIKR YQIDISKIGR LEVGTETIID KSKSVKSVLM QLFGDNHNVE GIDCVNACYG 130 140 150 160 170 180 GVNALFNTID WIESSAWDGR DGIVVAGDIA LYAKGNARPT GGAGCVALLV GPNAPIVFEP 190 200 210 220 230 240 GLRGTYMQHA YDFYKPDLTS EYPYVDGHFS LECYVKALDG AYANYNVRDV AKNGKSQGLG 250 260 270 280 290 300 LDRFDYCIFH APTCKQVQKA YARLLYTDSA AEPSNPELEG VRELLSTLDA KKSLTDKALE 310 320 330 340 350 360 KGLMAITKER FNKRVSPSVY APTNCGNMYT ASIFSCLTAL LSRVPADELK GKRVGAYSYG 370 380 390 400 410 420 SGLAASFFSF VVKGDVSEIA KKTNLVNDLD NRHCLTPTQY EEAIELRHQA HLKKNFTPKG 430 440 SIERLRSGTY YLTGIDDMFR RSYSVKP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and sequencing of the hcs gene, which encodes 3-hydroxy-3-methylglutaryl coenzyme A synthase of Schizosaccharomyces pombe." Katayama S., Adachi N., Takao K., Nakagawa T., Matsuda H., Kawamukai M. Yeast 11:1533-1537(1995) [PubMed: 8750242] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[3]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-398, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U32187 Genomic DNA. Translation: AAB17601.1. CU329670 Genomic DNA. Translation: CAB11060.1.
PIR	S61875.
RefSeq	NP_593859.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P54874.
Genome annotation databases
GeneID	2543141.
GenomeReviews	Gene locus hcs1 in contig CU329670_GR.
KEGG	spo:SPAC4F8.14c.
NMPDR	fig|4896.1.peg.3829.
Organism-specific databases
GeneDB_Spombe	SPAC4F8.14c.
Phylogenomic databases
OMA	CLTVVQN.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-001509-MON.
BRENDA	2.3.3.10. 653.
Gene expression databases
ArrayExpress	P54874.
Family and domain databases
InterPro	IPR000590. HMG_CoA_synt_AS. IPR013746. HMG_CoA_synt_C. IPR013528. HMG_CoA_synth_N. IPR010122. HMG_CoA_synthase_euk. [Graphical view]
Pfam	PF08540. HMG_CoA_synt_C. 1 hit. PF01154. HMG_CoA_synt_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01833. HMG-CoA-S_euk. 1 hit.
PROSITE	PS01226. HMG_COA_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMCS_SCHPO

Accession

Primary (citable) accession number: P54874

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 3, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)