Reviewed,
UniProtKB/Swiss-Prot P54873 (HMCS_ARATH)
Last modified
November 3, 2009.
Version 84.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Hydroxymethylglutaryl-CoA synthase Short name=HMG-CoA synthase EC=2.3.3.10 Alternative name(s): 3-hydroxy-3-methylglutaryl coenzyme A synthase Protein EMBRYO DEFECTIVE 2778 | ||||||||
| Gene names |
| ||||||||
| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 461 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase By similarity. |
| Catalytic activity | Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA. |
| Pathway | |
| Sequence similarities | Belongs to the HMG-CoA synthase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Lipid synthesis Steroid biosynthesis Sterol biosynthesis |
| Coding sequence diversity | Alternative splicing |
| Molecular function | Transferase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | isopentenyl diphosphate biosynthetic process, mevalonate pathway Traceable author statement. Source: TAIR sterol biosynthetic processInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | hydroxymethylglutaryl-CoA synthase activity Ref.1 Inferred from genetic interaction. Source: TAIR |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: P54873-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: P54873-2) The sequence of this isoform differs from the canonical sequence as follows: 1-55: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 461 | 461 | Hydroxymethylglutaryl-CoA synthase | PRO_0000213756 | |||||
Sites | |||||||||
| Active site | 117 | 1 | Potential | ||||||
Natural variations | |||||||||
| Alternative sequence | 1 – 55 | 55 | Missing in isoform 2. | VSP_008902 | |||||
Experimental info | |||||||||
| Sequence conflict | 306 | 1 | A → S in CAA58763. Ref.1 | ||||||
| Sequence conflict | 342 | 1 | K → N in CAA58763. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Isolation and characterization of a cDNA encoding Arabidopsis thaliana 3-hydroxy-3-methylglutaryl-coenzyme A synthase." Montamat F., Guilloton M., Karst F., Delrot S. Gene 167:197-201(1995) [PubMed: 8566777] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: cv. Landsberg erecta. |
| [2] | "Post-transcriptional regulation of HMG-CoA synthase expression in Arabidopsis thaliana." Connolly E.L., Learned R.M. Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1). |
| [3] | "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. McCombie W.R.Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [4] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| X83882 mRNA. Translation: CAA58763.1. U79160 mRNA. Translation: AAD00297.1. U79161 Genomic DNA. Translation: AAD00298.1. AL078606 Genomic DNA. Translation: CAB44320.1. AL161532 Genomic DNA. Translation: CAB78225.1. AY140008 mRNA. Translation: AAM98150.1. BT008492 mRNA. Translation: AAP37851.1. | |
| IPI | IPI00530283. IPI00533922. |
| PIR | T09341. |
| RefSeq | NP_192919.1. NP_849361.1. |
| UniGene | At.5362 |
3D structure databases | |
| SMR | P54873. Positions 2-451. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P54873. |
Proteomic databases | |
| PRIDE | P54873. |
Genome annotation databases | |
| GeneID | 826788. |
| GenomeReviews | Gene locus AT4G11820 in contig CT486007_GR. |
| KEGG | ath:AT4G11820. |
Organism-specific databases | |
| TAIR | At4g11820. |
Phylogenomic databases | |
| OMA | CLTVVQN. |
Enzyme and pathway databases | |
| BioCyc | MetaCyc:AT4G11820-MON. |
| BRENDA | 2.3.3.10. 302. |
Gene expression databases | |
| ArrayExpress | P54873. |
| Genevestigator | P54873. |
| GermOnline | AT4G11820. Arabidopsis thaliana. |
Family and domain databases | |
| InterPro | IPR000590. HMG_CoA_synt_AS. IPR013746. HMG_CoA_synt_C. IPR013528. HMG_CoA_synth_N. IPR010122. HMG_CoA_synthase_euk. [Graphical view] |
| Pfam | PF08540. HMG_CoA_synt_C. 1 hit. PF01154. HMG_CoA_synt_N. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR01833. HMG-CoA-S_euk. 1 hit. |
| PROSITE | PS01226. HMG_COA_SYNTHASE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | HMCS_ARATH | ||||||||
| Accession | Primary (citable) accession number: P54873 Secondary accession number(s): Q8L721, Q9S707 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


