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P54873

- HMCS_ARATH

UniProt

P54873 - HMCS_ARATH

Protein

Hydroxymethylglutaryl-CoA synthase

Gene

HMGS

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 2 (06 Jun 2002)
      Previous versions | rss
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    Functioni

    This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase. Devoided of acetoacetyl-CoA thiolase (AACT) activity. Required for the development of both tapetosomes and elaioplasts in tapetal cells and for pollen viability during pollen tube elongation.1 Publication

    Catalytic activityi

    Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.1 PublicationPROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei83 – 831Proton donor/acceptorPROSITE-ProRule annotation
    Active sitei117 – 1171Acyl-thioester intermediatePROSITE-ProRule annotation
    Active sitei247 – 2471Proton donor/acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. hydroxymethylglutaryl-CoA synthase activity Source: TAIR

    GO - Biological processi

    1. isopentenyl diphosphate biosynthetic process, mevalonate pathway Source: TAIR
    2. sterol biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

    Enzyme and pathway databases

    BioCyciARA:AT4G11820-MONOMER.
    ARA:GQT-1470-MONOMER.
    MetaCyc:AT4G11820-MONOMER.
    BRENDAi2.3.3.10. 399.
    ReactomeiREACT_187634. PPARA activates gene expression.
    UniPathwayiUPA00058; UER00102.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hydroxymethylglutaryl-CoA synthase (EC:2.3.3.10)
    Short name:
    HMG-CoA synthase
    Alternative name(s):
    3-hydroxy-3-methylglutaryl coenzyme A synthase
    Protein EMBRYO DEFECTIVE 2778
    Protein FLAKY POLLEN 1
    Gene namesi
    Name:HMGS
    Synonyms:EMB2778, FKP1, MVA1
    Ordered Locus Names:At4g11820
    ORF Names:T26M18.30
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G11820.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: TAIR
    2. plasmodesma Source: TAIR

    Pathology & Biotechi

    Disruption phenotypei

    Defect in pollen adhesion to the stigma resulting in male sterility under normal growth conditions.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 461461Hydroxymethylglutaryl-CoA synthasePRO_0000213756Add
    BLAST

    Proteomic databases

    PaxDbiP54873.
    PRIDEiP54873.

    Expressioni

    Tissue specificityi

    Highly expressed in flower buds, pollen grains and anthers in the tapetal cells, at an intermediate levels in roots and weakly in leaves.1 Publication

    Gene expression databases

    GenevestigatoriP54873.

    Structurei

    3D structure databases

    ProteinModelPortaliP54873.
    SMRiP54873. Positions 2-451.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the HMG-CoA synthase family.Curated

    Phylogenomic databases

    eggNOGiCOG3425.
    HOGENOMiHOG000012351.
    InParanoidiP54873.
    KOiK01641.
    OMAiDDAYNWI.
    PhylomeDBiP54873.

    Family and domain databases

    Gene3Di3.40.47.10. 2 hits.
    InterProiIPR000590. HMG_CoA_synt_AS.
    IPR013746. HMG_CoA_synt_C_dom.
    IPR013528. HMG_CoA_synth_N.
    IPR010122. HMG_CoA_synthase_euk.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view]
    PfamiPF08540. HMG_CoA_synt_C. 1 hit.
    PF01154. HMG_CoA_synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF53901. SSF53901. 3 hits.
    TIGRFAMsiTIGR01833. HMG-CoA-S_euk. 1 hit.
    PROSITEiPS01226. HMG_COA_SYNTHASE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P54873-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAKNVGILAM DIYFPPTCVQ QEALEAHDGA SKGKYTIGLG QDCLAFCTEL    50
    EDVISMSFNA VTSLFEKYKI DPNQIGRLEV GSETVIDKSK SIKTFLMQLF 100
    EKCGNTDVEG VDSTNACYGG TAALLNCVNW VESNSWDGRY GLVICTDSAV 150
    YAEGPARPTG GAAAIAMLIG PDAPIVFESK LRASHMAHVY DFYKPNLASE 200
    YPVVDGKLSQ TCYLMALDSC YKHLCNKFEK IEGKEFSIND ADYIVFHSPY 250
    NKLVQKSFAR LLYNDFLRNA SSIDEAAKEK FTPYSSLTLD ESYQSRDLEK 300
    VSQQIAKPFY DAKVQPTTLI PKEVGNMYTA SLYAAFASLI HKKHNDLAGK 350
    RVVMFSYGSG STATMFSLRL NDNKPPFSIS NIASVMDVGG KLKARHEYAP 400
    EKFVETMKLM EHRYGAKDFV TTKEGIIDLL APGTYYLKEV DSLYRRFYGK 450
    KGEDGSVANG H 461
    Length:461
    Mass (Da):51,094
    Last modified:June 6, 2002 - v2
    Checksum:iF44908369AFBC5A8
    GO
    Isoform 2 (identifier: P54873-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-55: Missing.

    Show »
    Length:406
    Mass (Da):45,205
    Checksum:i41CED5FD425BC13A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti306 – 3061A → S in CAA58763. (PubMed:8566777)Curated
    Sequence conflicti342 – 3421K → N in CAA58763. (PubMed:8566777)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 5555Missing in isoform 2. 1 PublicationVSP_008902Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X83882 mRNA. Translation: CAA58763.1.
    U79160 mRNA. Translation: AAD00297.1.
    U79161 Genomic DNA. Translation: AAD00298.1.
    AL078606 Genomic DNA. Translation: CAB44320.1.
    AL161532 Genomic DNA. Translation: CAB78225.1.
    CP002687 Genomic DNA. Translation: AEE83052.1.
    CP002687 Genomic DNA. Translation: AEE83053.1.
    AY140008 mRNA. Translation: AAM98150.1.
    BT008492 mRNA. Translation: AAP37851.1.
    PIRiT09341.
    RefSeqiNP_192919.1. NM_117251.2. [P54873-1]
    NP_849361.1. NM_179030.1. [P54873-2]
    UniGeneiAt.5362.

    Genome annotation databases

    EnsemblPlantsiAT4G11820.2; AT4G11820.2; AT4G11820. [P54873-1]
    GeneIDi826788.
    KEGGiath:AT4G11820.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X83882 mRNA. Translation: CAA58763.1 .
    U79160 mRNA. Translation: AAD00297.1 .
    U79161 Genomic DNA. Translation: AAD00298.1 .
    AL078606 Genomic DNA. Translation: CAB44320.1 .
    AL161532 Genomic DNA. Translation: CAB78225.1 .
    CP002687 Genomic DNA. Translation: AEE83052.1 .
    CP002687 Genomic DNA. Translation: AEE83053.1 .
    AY140008 mRNA. Translation: AAM98150.1 .
    BT008492 mRNA. Translation: AAP37851.1 .
    PIRi T09341.
    RefSeqi NP_192919.1. NM_117251.2. [P54873-1 ]
    NP_849361.1. NM_179030.1. [P54873-2 ]
    UniGenei At.5362.

    3D structure databases

    ProteinModelPortali P54873.
    SMRi P54873. Positions 2-451.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi P54873.
    PRIDEi P54873.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G11820.2 ; AT4G11820.2 ; AT4G11820 . [P54873-1 ]
    GeneIDi 826788.
    KEGGi ath:AT4G11820.

    Organism-specific databases

    TAIRi AT4G11820.

    Phylogenomic databases

    eggNOGi COG3425.
    HOGENOMi HOG000012351.
    InParanoidi P54873.
    KOi K01641.
    OMAi DDAYNWI.
    PhylomeDBi P54873.

    Enzyme and pathway databases

    UniPathwayi UPA00058 ; UER00102 .
    BioCyci ARA:AT4G11820-MONOMER.
    ARA:GQT-1470-MONOMER.
    MetaCyc:AT4G11820-MONOMER.
    BRENDAi 2.3.3.10. 399.
    Reactomei REACT_187634. PPARA activates gene expression.

    Gene expression databases

    Genevestigatori P54873.

    Family and domain databases

    Gene3Di 3.40.47.10. 2 hits.
    InterProi IPR000590. HMG_CoA_synt_AS.
    IPR013746. HMG_CoA_synt_C_dom.
    IPR013528. HMG_CoA_synth_N.
    IPR010122. HMG_CoA_synthase_euk.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view ]
    Pfami PF08540. HMG_CoA_synt_C. 1 hit.
    PF01154. HMG_CoA_synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53901. SSF53901. 3 hits.
    TIGRFAMsi TIGR01833. HMG-CoA-S_euk. 1 hit.
    PROSITEi PS01226. HMG_COA_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of a cDNA encoding Arabidopsis thaliana 3-hydroxy-3-methylglutaryl-coenzyme A synthase."
      Montamat F., Guilloton M., Karst F., Delrot S.
      Gene 167:197-201(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CATALYTIC ACTIVITY.
      Strain: cv. Landsberg erecta.
    2. "Post-transcriptional regulation of HMG-CoA synthase expression in Arabidopsis thaliana."
      Connolly E.L., Learned R.M.
      Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
    3. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: cv. Columbia.
    6. "The Arabidopsis FLAKY POLLEN1 gene encodes a 3-hydroxy-3-methylglutaryl-coenzyme A synthase required for development of tapetum-specific organelles and fertility of pollen grains."
      Ishiguro S., Nishimori Y., Yamada M., Saito H., Suzuki T., Nakagawa T., Miyake H., Okada K., Nakamura K.
      Plant Cell Physiol. 51:896-911(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiHMCS_ARATH
    AccessioniPrimary (citable) accession number: P54873
    Secondary accession number(s): Q8L721, Q9S707
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: June 6, 2002
    Last modified: October 1, 2014
    This is version 123 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3