P54873 (HMCS_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxymethylglutaryl-CoA synthase
Short name=HMG-CoA synthase
EC=2.3.3.10

Alternative name(s):
3-hydroxy-3-methylglutaryl coenzyme A synthase
Protein EMBRYO DEFECTIVE 2778

Gene names

Name:	HMGS
Synonyms:	EMB2778, MVA1
Ordered Locus Names:	At4g11820
ORF Names:	T26M18.30

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase By similarity.
Catalytic activity	Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.
Sequence similarities	Belongs to the HMG-CoA synthase family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Coding sequence diversity	Alternative splicing
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate pathway Traceable author statement. Source: TAIR sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from direct assay. Source: TAIR plasmodesma Inferred from direct assay. Source: TAIR
Molecular function	hydroxymethylglutaryl-CoA synthase activity Inferred from genetic interaction Ref.1. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P54873-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P54873-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-55: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 461	461	Hydroxymethylglutaryl-CoA synthase		PRO_0000213756
Sites
	Active site	117	1	Potential
Natural variations
	Alternative sequence	1 – 55	55	Missing in isoform 2.		VSP_008902
Experimental info
	Sequence conflict	306	1	A → S in CAA58763. Ref.1
	Sequence conflict	342	1	K → N in CAA58763. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 6, 2002. Version 2. Checksum: F44908369AFBC5A8	FASTA	461	51,094
	10 20 30 40 50 60 MAKNVGILAM DIYFPPTCVQ QEALEAHDGA SKGKYTIGLG QDCLAFCTEL EDVISMSFNA 70 80 90 100 110 120 VTSLFEKYKI DPNQIGRLEV GSETVIDKSK SIKTFLMQLF EKCGNTDVEG VDSTNACYGG 130 140 150 160 170 180 TAALLNCVNW VESNSWDGRY GLVICTDSAV YAEGPARPTG GAAAIAMLIG PDAPIVFESK 190 200 210 220 230 240 LRASHMAHVY DFYKPNLASE YPVVDGKLSQ TCYLMALDSC YKHLCNKFEK IEGKEFSIND 250 260 270 280 290 300 ADYIVFHSPY NKLVQKSFAR LLYNDFLRNA SSIDEAAKEK FTPYSSLTLD ESYQSRDLEK 310 320 330 340 350 360 VSQQIAKPFY DAKVQPTTLI PKEVGNMYTA SLYAAFASLI HKKHNDLAGK RVVMFSYGSG 370 380 390 400 410 420 STATMFSLRL NDNKPPFSIS NIASVMDVGG KLKARHEYAP EKFVETMKLM EHRYGAKDFV 430 440 450 460 TTKEGIIDLL APGTYYLKEV DSLYRRFYGK KGEDGSVANG H
	Isoform 2 [UniParc]. Checksum: 41CED5FD425BC13A	FASTA	406	45,205
	10 20 30 40 50 60 MSFNAVTSLF EKYKIDPNQI GRLEVGSETV IDKSKSIKTF LMQLFEKCGN TDVEGVDSTN 70 80 90 100 110 120 ACYGGTAALL NCVNWVESNS WDGRYGLVIC TDSAVYAEGP ARPTGGAAAI AMLIGPDAPI 130 140 150 160 170 180 VFESKLRASH MAHVYDFYKP NLASEYPVVD GKLSQTCYLM ALDSCYKHLC NKFEKIEGKE 190 200 210 220 230 240 FSINDADYIV FHSPYNKLVQ KSFARLLYND FLRNASSIDE AAKEKFTPYS SLTLDESYQS 250 260 270 280 290 300 RDLEKVSQQI AKPFYDAKVQ PTTLIPKEVG NMYTASLYAA FASLIHKKHN DLAGKRVVMF 310 320 330 340 350 360 SYGSGSTATM FSLRLNDNKP PFSISNIASV MDVGGKLKAR HEYAPEKFVE TMKLMEHRYG 370 380 390 400 AKDFVTTKEG IIDLLAPGTY YLKEVDSLYR RFYGKKGEDG SVANGH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of a cDNA encoding Arabidopsis thaliana 3-hydroxy-3-methylglutaryl-coenzyme A synthase." Montamat F., Guilloton M., Karst F., Delrot S. Gene 167:197-201(1995) [PubMed: 8566777] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: cv. Landsberg erecta.
[2]	"Post-transcriptional regulation of HMG-CoA synthase expression in Arabidopsis thaliana." Connolly E.L., Learned R.M. Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
[3]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X83882 mRNA. Translation: CAA58763.1. U79160 mRNA. Translation: AAD00297.1. U79161 Genomic DNA. Translation: AAD00298.1. AL078606 Genomic DNA. Translation: CAB44320.1. AL161532 Genomic DNA. Translation: CAB78225.1. CP002687 Genomic DNA. Translation: AEE83052.1. CP002687 Genomic DNA. Translation: AEE83053.1. AY140008 mRNA. Translation: AAM98150.1. BT008492 mRNA. Translation: AAP37851.1.
IPI	IPI00530283. IPI00533922.
PIR	T09341.
RefSeq	NP_192919.1. NM_117251.2. NP_849361.1. NM_179030.1.
UniGene	At.5362.
3D structure databases
ProteinModelPortal	P54873.
SMR	P54873. Positions 2-451.
ModBase	Search...
Protein-protein interaction databases
STRING	P54873.
Proteomic databases
PRIDE	P54873.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G11820.2; AT4G11820.2; AT4G11820.
GeneID	826788.
GenomeReviews	Gene locus AT4G11820 in contig CT486007_GR.
KEGG	ath:AT4G11820.
Organism-specific databases
TAIR	At4g11820.
Phylogenomic databases
eggNOG	KOG1393.
GeneTree	EPGT00050000009656.
HOGENOM	HBG578402.
InParanoid	P54873.
OMA	DTVNACY.
PhylomeDB	P54873.
ProtClustDB	PLN02577.
Enzyme and pathway databases
BioCyc	ARA:AT4G11820-MONOMER. MetaCyc:AT4G11820-MONOMER.
BRENDA	2.3.3.10. 399.
Gene expression databases
ArrayExpress	P54873.
Genevestigator	P54873.
GermOnline	AT4G11820. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000590. HMG_CoA_synt_AS. IPR013746. HMG_CoA_synt_C. IPR013528. HMG_CoA_synth_N. IPR010122. HMG_CoA_synthase_euk. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 2 hits.
KO	K01641.
Pfam	PF08540. HMG_CoA_synt_C. 1 hit. PF01154. HMG_CoA_synt_N. 1 hit. [Graphical view]
SUPFAM	SSF53901. Thiolase-like. 2 hits.
TIGRFAMs	TIGR01833. HMG-CoA-S_euk. 1 hit.
PROSITE	PS01226. HMG_COA_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMCS_ARATH

Accession

Primary (citable) accession number: P54873
Secondary accession number(s): Q8L721, Q9S707

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	June 6, 2002
Last modified:	November 16, 2011
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program