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P54873 (HMCS_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxymethylglutaryl-CoA synthase

Short name=HMG-CoA synthase
EC=2.3.3.10
Alternative name(s):
3-hydroxy-3-methylglutaryl coenzyme A synthase
Protein EMBRYO DEFECTIVE 2778
Protein FLAKY POLLEN 1
Gene names
Name:HMGS
Synonyms:EMB2778, FKP1, MVA1
Ordered Locus Names:At4g11820
ORF Names:T26M18.30
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length461 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase. Devoided of acetoacetyl-CoA thiolase (AACT) activity. Required for the development of both tapetosomes and elaioplasts in tapetal cells and for pollen viability during pollen tube elongation. Ref.6

Catalytic activity

Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA. Ref.1

Pathway

Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.

Tissue specificity

Highly expressed in flower buds, pollen grains and anthers in the tapetal cells, at an intermediate levels in roots and weakly in leaves. Ref.6

Disruption phenotype

Defect in pollen adhesion to the stigma resulting in male sterility under normal growth conditions. Ref.6

Sequence similarities

Belongs to the HMG-CoA synthase family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P54873-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P54873-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-55: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 461461Hydroxymethylglutaryl-CoA synthase
PRO_0000213756

Sites

Active site831Proton donor/acceptor By similarity
Active site1171Acyl-thioester intermediate By similarity
Active site2471Proton donor/acceptor By similarity

Natural variations

Alternative sequence1 – 5555Missing in isoform 2.
VSP_008902

Experimental info

Sequence conflict3061A → S in CAA58763. Ref.1
Sequence conflict3421K → N in CAA58763. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 6, 2002. Version 2.
Checksum: F44908369AFBC5A8

FASTA46151,094
        10         20         30         40         50         60 
MAKNVGILAM DIYFPPTCVQ QEALEAHDGA SKGKYTIGLG QDCLAFCTEL EDVISMSFNA 

        70         80         90        100        110        120 
VTSLFEKYKI DPNQIGRLEV GSETVIDKSK SIKTFLMQLF EKCGNTDVEG VDSTNACYGG 

       130        140        150        160        170        180 
TAALLNCVNW VESNSWDGRY GLVICTDSAV YAEGPARPTG GAAAIAMLIG PDAPIVFESK 

       190        200        210        220        230        240 
LRASHMAHVY DFYKPNLASE YPVVDGKLSQ TCYLMALDSC YKHLCNKFEK IEGKEFSIND 

       250        260        270        280        290        300 
ADYIVFHSPY NKLVQKSFAR LLYNDFLRNA SSIDEAAKEK FTPYSSLTLD ESYQSRDLEK 

       310        320        330        340        350        360 
VSQQIAKPFY DAKVQPTTLI PKEVGNMYTA SLYAAFASLI HKKHNDLAGK RVVMFSYGSG 

       370        380        390        400        410        420 
STATMFSLRL NDNKPPFSIS NIASVMDVGG KLKARHEYAP EKFVETMKLM EHRYGAKDFV 

       430        440        450        460 
TTKEGIIDLL APGTYYLKEV DSLYRRFYGK KGEDGSVANG H 

« Hide

Isoform 2 [UniParc].

Checksum: 41CED5FD425BC13A
Show »

FASTA40645,205

References

« Hide 'large scale' references
[1]"Isolation and characterization of a cDNA encoding Arabidopsis thaliana 3-hydroxy-3-methylglutaryl-coenzyme A synthase."
Montamat F., Guilloton M., Karst F., Delrot S.
Gene 167:197-201(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CATALYTIC ACTIVITY.
Strain: cv. Landsberg erecta.
[2]"Post-transcriptional regulation of HMG-CoA synthase expression in Arabidopsis thaliana."
Connolly E.L., Learned R.M.
Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
[3]"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. expand/collapse author list , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: cv. Columbia.
[6]"The Arabidopsis FLAKY POLLEN1 gene encodes a 3-hydroxy-3-methylglutaryl-coenzyme A synthase required for development of tapetum-specific organelles and fertility of pollen grains."
Ishiguro S., Nishimori Y., Yamada M., Saito H., Suzuki T., Nakagawa T., Miyake H., Okada K., Nakamura K.
Plant Cell Physiol. 51:896-911(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X83882 mRNA. Translation: CAA58763.1.
U79160 mRNA. Translation: AAD00297.1.
U79161 Genomic DNA. Translation: AAD00298.1.
AL078606 Genomic DNA. Translation: CAB44320.1.
AL161532 Genomic DNA. Translation: CAB78225.1.
CP002687 Genomic DNA. Translation: AEE83052.1.
CP002687 Genomic DNA. Translation: AEE83053.1.
AY140008 mRNA. Translation: AAM98150.1.
BT008492 mRNA. Translation: AAP37851.1.
PIRT09341.
RefSeqNP_192919.1. NM_117251.2. [P54873-1]
NP_849361.1. NM_179030.1. [P54873-2]
UniGeneAt.5362.

3D structure databases

ProteinModelPortalP54873.
SMRP54873. Positions 2-451.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbP54873.
PRIDEP54873.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT4G11820.2; AT4G11820.2; AT4G11820. [P54873-1]
GeneID826788.
KEGGath:AT4G11820.

Organism-specific databases

TAIRAT4G11820.

Phylogenomic databases

eggNOGCOG3425.
HOGENOMHOG000012351.
InParanoidP54873.
KOK01641.
OMADDAYNWI.
PhylomeDBP54873.

Enzyme and pathway databases

BioCycARA:AT4G11820-MONOMER.
ARA:GQT-1470-MONOMER.
MetaCyc:AT4G11820-MONOMER.
BRENDA2.3.3.10. 399.
UniPathwayUPA00058; UER00102.

Gene expression databases

GenevestigatorP54873.

Family and domain databases

Gene3D3.40.47.10. 2 hits.
InterProIPR000590. HMG_CoA_synt_AS.
IPR013746. HMG_CoA_synt_C.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamPF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view]
SUPFAMSSF53901. SSF53901. 3 hits.
TIGRFAMsTIGR01833. HMG-CoA-S_euk. 1 hit.
PROSITEPS01226. HMG_COA_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHMCS_ARATH
AccessionPrimary (citable) accession number: P54873
Secondary accession number(s): Q8L721, Q9S707
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: June 6, 2002
Last modified: June 11, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names