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P54872

- HMCSA_DICDI

UniProt

P54872 - HMCSA_DICDI

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Protein
Hydroxymethylglutaryl-CoA synthase A
Gene
hgsA, DDB_G0288461
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase By similarity.

Catalytic activityi

Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei85 – 851Proton donor/acceptor By similarity
Active sitei119 – 1191Acyl-thioester intermediate By similarity
Active sitei249 – 2491Proton donor/acceptor By similarity

GO - Molecular functioni

  1. hydroxymethylglutaryl-CoA synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. isoprenoid biosynthetic process Source: InterPro
  2. sterol biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Enzyme and pathway databases

ReactomeiREACT_204470. PPARA activates gene expression.
REACT_205241. Activation of gene expression by SREBF (SREBP).
REACT_227623. Cholesterol biosynthesis.
UniPathwayiUPA00058; UER00102.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxymethylglutaryl-CoA synthase A (EC:2.3.3.10)
Short name:
HMG-CoA synthase A
Alternative name(s):
3-hydroxy-3-methylglutaryl coenzyme A synthase A
Gene namesi
Name:hgsA
ORF Names:DDB_G0288461
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0288461. hgsA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 482482Hydroxymethylglutaryl-CoA synthase A
PRO_0000213755Add
BLAST

Proteomic databases

PRIDEiP54872.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0219924.

Structurei

3D structure databases

ProteinModelPortaliP54872.
SMRiP54872. Positions 3-443.

Family & Domainsi

Sequence similaritiesi

Belongs to the HMG-CoA synthase family.

Phylogenomic databases

eggNOGiCOG3425.
KOiK01641.
OMAiPINHIVE.
PhylomeDBiP54872.

Family and domain databases

Gene3Di3.40.47.10. 1 hit.
InterProiIPR013746. HMG_CoA_synt_C_dom.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF53901. SSF53901. 3 hits.
TIGRFAMsiTIGR01833. HMG-CoA-S_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

P54872-1 [UniParc]FASTAAdd to Basket

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MTKPENIGIH GIEVYFPSTY VAQEDLEKFD GVSQGKYTLG LGQTNMAFCG    50
DREDIYSLSL NAVNNLMDKF NVDPNSIGRL EVGTETVIDK SKSVKTVLMD 100
LFAKHGNTSI DGIDTINACY GGTSALHNAL QWMESSYWDG RNAIVVAGDI 150
AVYEKGPARP TGGAGVVAML IGPNAPITFE SGLRGVHMEN VYDFYKPDMD 200
SEYPRVDGKL SISCYFRAID NCYNRYAKAF EKKYGKSFSL DQVDFALFHS 250
PYNKLVQKSF GRMLYNDFLN NPNDSRYASL EAYKNVKPED TYFDSVLEKA 300
LSAITKNDYA TKVAPTTLLA KQLGNTYCGS TYSGLLSLLD EKSNDLVGKR 350
VLTFSYGSGL AASAFSFKVE KPINHIVEKV DLKNRLAKRV RVEPEIFTEK 400
LSLRETRHNL KNYVPSDETT NMFPGSFYLS SVDNAGIRKY DRTYSTSAVL 450
GAFQRRQQIS QSTIKSLNLF RATKSVLSIL KK 482
Length:482
Mass (Da):53,741
Last modified:December 4, 2007 - v2
Checksum:iE6A00A6064B765AF
GO

Sequence cautioni

The sequence AAA91055.1 differs from that shown. Reason: Frameshift at positions 461 and 482.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti304 – 3063ITK → RHE in AAA91055. 1 Publication
Sequence conflicti434 – 4341N → K in AAA91055. 1 Publication
Sequence conflicti440 – 4401Y → F in AAA91055. 1 Publication
Sequence conflicti461 – 4666QSTIKS → HQQSNL in AAA91055. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000111 Genomic DNA. Translation: EAL63202.1.
L24114 mRNA. Translation: AAA91055.1. Frameshift.
RefSeqiXP_636713.1. XM_631621.1.

Genome annotation databases

EnsemblProtistsiDDB0219924; DDB0219924; DDB_G0288461.
GeneIDi8626645.
KEGGiddi:DDB_G0288461.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000111 Genomic DNA. Translation: EAL63202.1 .
L24114 mRNA. Translation: AAA91055.1 . Frameshift.
RefSeqi XP_636713.1. XM_631621.1.

3D structure databases

ProteinModelPortali P54872.
SMRi P54872. Positions 3-443.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0219924.

Proteomic databases

PRIDEi P54872.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0219924 ; DDB0219924 ; DDB_G0288461 .
GeneIDi 8626645.
KEGGi ddi:DDB_G0288461.

Organism-specific databases

dictyBasei DDB_G0288461. hgsA.

Phylogenomic databases

eggNOGi COG3425.
KOi K01641.
OMAi PINHIVE.
PhylomeDBi P54872.

Enzyme and pathway databases

UniPathwayi UPA00058 ; UER00102 .
Reactomei REACT_204470. PPARA activates gene expression.
REACT_205241. Activation of gene expression by SREBF (SREBP).
REACT_227623. Cholesterol biosynthesis.

Family and domain databases

Gene3Di 3.40.47.10. 1 hit.
InterProi IPR013746. HMG_CoA_synt_C_dom.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view ]
Pfami PF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view ]
SUPFAMi SSF53901. SSF53901. 3 hits.
TIGRFAMsi TIGR01833. HMG-CoA-S_euk. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. Bienvenut W.V., Ura S., Insall R.H.
    Submitted (JUL-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 96-104; 263-276; 300-321 AND 390-400, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: AX2.
  3. "A putative partial cDNA clone of HMG-CoA synthase from Dictyostelium discoideum."
    Dhar M.S., Hauser L.J., Olins D.E., Olins A.L.
    Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 304-482.

Entry informationi

Entry nameiHMCSA_DICDI
AccessioniPrimary (citable) accession number: P54872
Secondary accession number(s): Q54IW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 4, 2007
Last modified: September 3, 2014
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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