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P54872

- HMCSA_DICDI

UniProt

P54872 - HMCSA_DICDI

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Protein

Hydroxymethylglutaryl-CoA synthase A

Gene

hgsA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.By similarity

Catalytic activityi

Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei85 – 851Proton donor/acceptorBy similarity
Active sitei119 – 1191Acyl-thioester intermediateBy similarity
Active sitei249 – 2491Proton donor/acceptorBy similarity

GO - Molecular functioni

  1. hydroxymethylglutaryl-CoA synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. isoprenoid biosynthetic process Source: InterPro
  2. sterol biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Enzyme and pathway databases

ReactomeiREACT_204470. PPARA activates gene expression.
REACT_205241. Activation of gene expression by SREBF (SREBP).
REACT_227623. Cholesterol biosynthesis.
UniPathwayiUPA00058; UER00102.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxymethylglutaryl-CoA synthase A (EC:2.3.3.10)
Short name:
HMG-CoA synthase A
Alternative name(s):
3-hydroxy-3-methylglutaryl coenzyme A synthase A
Gene namesi
Name:hgsA
ORF Names:DDB_G0288461
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0288461. hgsA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 482482Hydroxymethylglutaryl-CoA synthase APRO_0000213755Add
BLAST

Proteomic databases

PRIDEiP54872.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0219924.

Structurei

3D structure databases

ProteinModelPortaliP54872.
SMRiP54872. Positions 3-443.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HMG-CoA synthase family.Curated

Phylogenomic databases

eggNOGiCOG3425.
InParanoidiP54872.
KOiK01641.
OMAiPINHIVE.
PhylomeDBiP54872.

Family and domain databases

Gene3Di3.40.47.10. 1 hit.
InterProiIPR013746. HMG_CoA_synt_C_dom.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF53901. SSF53901. 3 hits.
TIGRFAMsiTIGR01833. HMG-CoA-S_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

P54872-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTKPENIGIH GIEVYFPSTY VAQEDLEKFD GVSQGKYTLG LGQTNMAFCG
60 70 80 90 100
DREDIYSLSL NAVNNLMDKF NVDPNSIGRL EVGTETVIDK SKSVKTVLMD
110 120 130 140 150
LFAKHGNTSI DGIDTINACY GGTSALHNAL QWMESSYWDG RNAIVVAGDI
160 170 180 190 200
AVYEKGPARP TGGAGVVAML IGPNAPITFE SGLRGVHMEN VYDFYKPDMD
210 220 230 240 250
SEYPRVDGKL SISCYFRAID NCYNRYAKAF EKKYGKSFSL DQVDFALFHS
260 270 280 290 300
PYNKLVQKSF GRMLYNDFLN NPNDSRYASL EAYKNVKPED TYFDSVLEKA
310 320 330 340 350
LSAITKNDYA TKVAPTTLLA KQLGNTYCGS TYSGLLSLLD EKSNDLVGKR
360 370 380 390 400
VLTFSYGSGL AASAFSFKVE KPINHIVEKV DLKNRLAKRV RVEPEIFTEK
410 420 430 440 450
LSLRETRHNL KNYVPSDETT NMFPGSFYLS SVDNAGIRKY DRTYSTSAVL
460 470 480
GAFQRRQQIS QSTIKSLNLF RATKSVLSIL KK
Length:482
Mass (Da):53,741
Last modified:December 4, 2007 - v2
Checksum:iE6A00A6064B765AF
GO

Sequence cautioni

The sequence AAA91055.1 differs from that shown. Reason: Frameshift at positions 461 and 482.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti304 – 3063ITK → RHE in AAA91055. 1 PublicationCurated
Sequence conflicti434 – 4341N → K in AAA91055. 1 PublicationCurated
Sequence conflicti440 – 4401Y → F in AAA91055. 1 PublicationCurated
Sequence conflicti461 – 4666QSTIKS → HQQSNL in AAA91055. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000111 Genomic DNA. Translation: EAL63202.1.
L24114 mRNA. Translation: AAA91055.1. Frameshift.
RefSeqiXP_636713.1. XM_631621.1.

Genome annotation databases

EnsemblProtistsiDDB0219924; DDB0219924; DDB_G0288461.
GeneIDi8626645.
KEGGiddi:DDB_G0288461.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000111 Genomic DNA. Translation: EAL63202.1 .
L24114 mRNA. Translation: AAA91055.1 . Frameshift.
RefSeqi XP_636713.1. XM_631621.1.

3D structure databases

ProteinModelPortali P54872.
SMRi P54872. Positions 3-443.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0219924.

Proteomic databases

PRIDEi P54872.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0219924 ; DDB0219924 ; DDB_G0288461 .
GeneIDi 8626645.
KEGGi ddi:DDB_G0288461.

Organism-specific databases

dictyBasei DDB_G0288461. hgsA.

Phylogenomic databases

eggNOGi COG3425.
InParanoidi P54872.
KOi K01641.
OMAi PINHIVE.
PhylomeDBi P54872.

Enzyme and pathway databases

UniPathwayi UPA00058 ; UER00102 .
Reactomei REACT_204470. PPARA activates gene expression.
REACT_205241. Activation of gene expression by SREBF (SREBP).
REACT_227623. Cholesterol biosynthesis.

Family and domain databases

Gene3Di 3.40.47.10. 1 hit.
InterProi IPR013746. HMG_CoA_synt_C_dom.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view ]
Pfami PF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view ]
SUPFAMi SSF53901. SSF53901. 3 hits.
TIGRFAMsi TIGR01833. HMG-CoA-S_euk. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. Bienvenut W.V., Ura S., Insall R.H.
    Submitted (JUL-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 96-104; 263-276; 300-321 AND 390-400, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: AX2.
  3. "A putative partial cDNA clone of HMG-CoA synthase from Dictyostelium discoideum."
    Dhar M.S., Hauser L.J., Olins D.E., Olins A.L.
    Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 304-482.

Entry informationi

Entry nameiHMCSA_DICDI
AccessioniPrimary (citable) accession number: P54872
Secondary accession number(s): Q54IW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 4, 2007
Last modified: October 29, 2014
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3