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P54872

- HMCSA_DICDI

UniProt

P54872 - HMCSA_DICDI

Protein

Hydroxymethylglutaryl-CoA synthase A

Gene

hgsA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 2 (04 Dec 2007)
      Previous versions | rss
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    Functioni

    Condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.By similarity

    Catalytic activityi

    Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei85 – 851Proton donor/acceptorBy similarity
    Active sitei119 – 1191Acyl-thioester intermediateBy similarity
    Active sitei249 – 2491Proton donor/acceptorBy similarity

    GO - Molecular functioni

    1. hydroxymethylglutaryl-CoA synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. isoprenoid biosynthetic process Source: InterPro
    2. sterol biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

    Enzyme and pathway databases

    ReactomeiREACT_204470. PPARA activates gene expression.
    REACT_205241. Activation of gene expression by SREBF (SREBP).
    REACT_227623. Cholesterol biosynthesis.
    UniPathwayiUPA00058; UER00102.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hydroxymethylglutaryl-CoA synthase A (EC:2.3.3.10)
    Short name:
    HMG-CoA synthase A
    Alternative name(s):
    3-hydroxy-3-methylglutaryl coenzyme A synthase A
    Gene namesi
    Name:hgsA
    ORF Names:DDB_G0288461
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0288461. hgsA.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 482482Hydroxymethylglutaryl-CoA synthase APRO_0000213755Add
    BLAST

    Proteomic databases

    PRIDEiP54872.

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0219924.

    Structurei

    3D structure databases

    ProteinModelPortaliP54872.
    SMRiP54872. Positions 3-443.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the HMG-CoA synthase family.Curated

    Phylogenomic databases

    eggNOGiCOG3425.
    KOiK01641.
    OMAiPINHIVE.
    PhylomeDBiP54872.

    Family and domain databases

    Gene3Di3.40.47.10. 1 hit.
    InterProiIPR013746. HMG_CoA_synt_C_dom.
    IPR013528. HMG_CoA_synth_N.
    IPR010122. HMG_CoA_synthase_euk.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view]
    PfamiPF08540. HMG_CoA_synt_C. 1 hit.
    PF01154. HMG_CoA_synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF53901. SSF53901. 3 hits.
    TIGRFAMsiTIGR01833. HMG-CoA-S_euk. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P54872-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTKPENIGIH GIEVYFPSTY VAQEDLEKFD GVSQGKYTLG LGQTNMAFCG    50
    DREDIYSLSL NAVNNLMDKF NVDPNSIGRL EVGTETVIDK SKSVKTVLMD 100
    LFAKHGNTSI DGIDTINACY GGTSALHNAL QWMESSYWDG RNAIVVAGDI 150
    AVYEKGPARP TGGAGVVAML IGPNAPITFE SGLRGVHMEN VYDFYKPDMD 200
    SEYPRVDGKL SISCYFRAID NCYNRYAKAF EKKYGKSFSL DQVDFALFHS 250
    PYNKLVQKSF GRMLYNDFLN NPNDSRYASL EAYKNVKPED TYFDSVLEKA 300
    LSAITKNDYA TKVAPTTLLA KQLGNTYCGS TYSGLLSLLD EKSNDLVGKR 350
    VLTFSYGSGL AASAFSFKVE KPINHIVEKV DLKNRLAKRV RVEPEIFTEK 400
    LSLRETRHNL KNYVPSDETT NMFPGSFYLS SVDNAGIRKY DRTYSTSAVL 450
    GAFQRRQQIS QSTIKSLNLF RATKSVLSIL KK 482
    Length:482
    Mass (Da):53,741
    Last modified:December 4, 2007 - v2
    Checksum:iE6A00A6064B765AF
    GO

    Sequence cautioni

    The sequence AAA91055.1 differs from that shown. Reason: Frameshift at positions 461 and 482.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti304 – 3063ITK → RHE in AAA91055. 1 PublicationCurated
    Sequence conflicti434 – 4341N → K in AAA91055. 1 PublicationCurated
    Sequence conflicti440 – 4401Y → F in AAA91055. 1 PublicationCurated
    Sequence conflicti461 – 4666QSTIKS → HQQSNL in AAA91055. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000111 Genomic DNA. Translation: EAL63202.1.
    L24114 mRNA. Translation: AAA91055.1. Frameshift.
    RefSeqiXP_636713.1. XM_631621.1.

    Genome annotation databases

    EnsemblProtistsiDDB0219924; DDB0219924; DDB_G0288461.
    GeneIDi8626645.
    KEGGiddi:DDB_G0288461.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000111 Genomic DNA. Translation: EAL63202.1 .
    L24114 mRNA. Translation: AAA91055.1 . Frameshift.
    RefSeqi XP_636713.1. XM_631621.1.

    3D structure databases

    ProteinModelPortali P54872.
    SMRi P54872. Positions 3-443.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0219924.

    Proteomic databases

    PRIDEi P54872.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0219924 ; DDB0219924 ; DDB_G0288461 .
    GeneIDi 8626645.
    KEGGi ddi:DDB_G0288461.

    Organism-specific databases

    dictyBasei DDB_G0288461. hgsA.

    Phylogenomic databases

    eggNOGi COG3425.
    KOi K01641.
    OMAi PINHIVE.
    PhylomeDBi P54872.

    Enzyme and pathway databases

    UniPathwayi UPA00058 ; UER00102 .
    Reactomei REACT_204470. PPARA activates gene expression.
    REACT_205241. Activation of gene expression by SREBF (SREBP).
    REACT_227623. Cholesterol biosynthesis.

    Family and domain databases

    Gene3Di 3.40.47.10. 1 hit.
    InterProi IPR013746. HMG_CoA_synt_C_dom.
    IPR013528. HMG_CoA_synth_N.
    IPR010122. HMG_CoA_synthase_euk.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view ]
    Pfami PF08540. HMG_CoA_synt_C. 1 hit.
    PF01154. HMG_CoA_synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53901. SSF53901. 3 hits.
    TIGRFAMsi TIGR01833. HMG-CoA-S_euk. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. Bienvenut W.V., Ura S., Insall R.H.
      Submitted (JUL-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 96-104; 263-276; 300-321 AND 390-400, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: AX2.
    3. "A putative partial cDNA clone of HMG-CoA synthase from Dictyostelium discoideum."
      Dhar M.S., Hauser L.J., Olins D.E., Olins A.L.
      Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 304-482.

    Entry informationi

    Entry nameiHMCSA_DICDI
    AccessioniPrimary (citable) accession number: P54872
    Secondary accession number(s): Q54IW3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 88 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3