P54872 (HMCSA_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxymethylglutaryl-CoA synthase A
Short name=HMG-CoA synthase A
EC=2.3.3.10

Alternative name(s):
3-hydroxy-3-methylglutaryl coenzyme A synthase A

Gene names

Name:	hgsA
ORF Names:	DDB_G0288461

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase By similarity.
Catalytic activity	Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.
Sequence similarities	Belongs to the HMG-CoA synthase family.
Sequence caution	The sequence AAA91055.1 differs from that shown. Reason: Frameshift at positions 461 and 482.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Steroid biosynthesis Steroid metabolism Sterol biosynthesis Sterol metabolism
Molecular function	Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: InterPro sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	hydroxymethylglutaryl-CoA synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 482	482	Hydroxymethylglutaryl-CoA synthase A		PRO_0000213755
Experimental info
	Sequence conflict	304 – 306	3	ITK → RHE in AAA91055. Ref.3
	Sequence conflict	434	1	N → K in AAA91055. Ref.3
	Sequence conflict	440	1	Y → F in AAA91055. Ref.3
	Sequence conflict	461 – 466	6	QSTIKS → HQQSNL in AAA91055. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P54872 [UniParc]. Last modified December 4, 2007. Version 2. Checksum: E6A00A6064B765AF	FASTA	482	53,741
	10 20 30 40 50 60 MTKPENIGIH GIEVYFPSTY VAQEDLEKFD GVSQGKYTLG LGQTNMAFCG DREDIYSLSL 70 80 90 100 110 120 NAVNNLMDKF NVDPNSIGRL EVGTETVIDK SKSVKTVLMD LFAKHGNTSI DGIDTINACY 130 140 150 160 170 180 GGTSALHNAL QWMESSYWDG RNAIVVAGDI AVYEKGPARP TGGAGVVAML IGPNAPITFE 190 200 210 220 230 240 SGLRGVHMEN VYDFYKPDMD SEYPRVDGKL SISCYFRAID NCYNRYAKAF EKKYGKSFSL 250 260 270 280 290 300 DQVDFALFHS PYNKLVQKSF GRMLYNDFLN NPNDSRYASL EAYKNVKPED TYFDSVLEKA 310 320 330 340 350 360 LSAITKNDYA TKVAPTTLLA KQLGNTYCGS TYSGLLSLLD EKSNDLVGKR VLTFSYGSGL 370 380 390 400 410 420 AASAFSFKVE KPINHIVEKV DLKNRLAKRV RVEPEIFTEK LSLRETRHNL KNYVPSDETT 430 440 450 460 470 480 NMFPGSFYLS SVDNAGIRKY DRTYSTSAVL GAFQRRQQIS QSTIKSLNLF RATKSVLSIL KK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	Bienvenut W.V., Ura S., Insall R.H. Submitted (JUL-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 96-104; 263-276; 300-321 AND 390-400, MASS SPECTROMETRY. Strain: AX2.
[3]	"A putative partial cDNA clone of HMG-CoA synthase from Dictyostelium discoideum." Dhar M.S., Hauser L.J., Olins D.E., Olins A.L. Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 304-482.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000111 Genomic DNA. Translation: EAL63202.1. L24114 mRNA. Translation: AAA91055.1. Frameshift.
RefSeq	XP_636713.1. XM_631621.1.
3D structure databases
ProteinModelPortal	P54872.
SMR	P54872. Positions 3-443.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0219924.
Proteomic databases
PRIDE	P54872.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0219924; DDB0219924; DDB_G0288461.
GeneID	8626645.
KEGG	ddi:DDB_G0288461.
Organism-specific databases
dictyBase	DDB_G0288461. hgsA.
Phylogenomic databases
eggNOG	COG3425.
KO	K01641.
OMA	RDFTLND.
ProtClustDB	CLSZ2429883.
Enzyme and pathway databases
UniPathway	UPA00058; UER00102.
Family and domain databases
Gene3D	3.40.47.10. 1 hit.
InterPro	IPR013746. HMG_CoA_synt_C. IPR013528. HMG_CoA_synth_N. IPR010122. HMG_CoA_synthase_euk. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Pfam	PF08540. HMG_CoA_synt_C. 1 hit. PF01154. HMG_CoA_synt_N. 1 hit. [Graphical view]
SUPFAM	SSF53901. Thiolase-like. 2 hits.
TIGRFAMs	TIGR01833. HMG-CoA-S_euk. 1 hit.
PROSITE	PS01226. HMG_COA_SYNTHASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMCSA_DICDI

Accession

Primary (citable) accession number: P54872
Secondary accession number(s): Q54IW3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	December 4, 2007
Last modified:	May 1, 2013
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)