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Protein

Hydroxymethylglutaryl-CoA synthase A

Gene

hgsA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.By similarity

Catalytic activityi

Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.

Pathwayi: (R)-mevalonate biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes (R)-mevalonate from acetyl-CoA.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Hydroxymethylglutaryl-CoA synthase A (hgsA), Hydroxymethylglutaryl-CoA synthase B (hgsB)
  3. 3-hydroxy-3-methylglutaryl-coenzyme A reductase 1 (hmgA), 3-hydroxy-3-methylglutaryl-coenzyme A reductase 2 (hmgB)
This subpathway is part of the pathway (R)-mevalonate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-mevalonate from acetyl-CoA, the pathway (R)-mevalonate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei85Proton donor/acceptorBy similarity1
Active sitei119Acyl-thioester intermediateBy similarity1
Active sitei249Proton donor/acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Enzyme and pathway databases

ReactomeiR-DDI-191273. Cholesterol biosynthesis.
R-DDI-77111. Synthesis of Ketone Bodies.
UniPathwayiUPA00058; UER00102.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxymethylglutaryl-CoA synthase A (EC:2.3.3.10)
Short name:
HMG-CoA synthase A
Alternative name(s):
3-hydroxy-3-methylglutaryl coenzyme A synthase A
Gene namesi
Name:hgsA
ORF Names:DDB_G0288461
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 5, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0288461. hgsA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002137551 – 482Hydroxymethylglutaryl-CoA synthase AAdd BLAST482

Proteomic databases

PaxDbiP54872.
PRIDEiP54872.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0219924.

Structurei

3D structure databases

ProteinModelPortaliP54872.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HMG-CoA synthase family.Curated

Phylogenomic databases

eggNOGiKOG1393. Eukaryota.
COG3425. LUCA.
InParanoidiP54872.
KOiK01641.
OMAiQRCISEE.
PhylomeDBiP54872.

Family and domain databases

Gene3Di3.40.47.10. 1 hit.
InterProiIPR013746. HMG_CoA_synt_C_dom.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
[Graphical view]
PfamiPF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF53901. SSF53901. 3 hits.
TIGRFAMsiTIGR01833. HMG-CoA-S_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

P54872-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKPENIGIH GIEVYFPSTY VAQEDLEKFD GVSQGKYTLG LGQTNMAFCG
60 70 80 90 100
DREDIYSLSL NAVNNLMDKF NVDPNSIGRL EVGTETVIDK SKSVKTVLMD
110 120 130 140 150
LFAKHGNTSI DGIDTINACY GGTSALHNAL QWMESSYWDG RNAIVVAGDI
160 170 180 190 200
AVYEKGPARP TGGAGVVAML IGPNAPITFE SGLRGVHMEN VYDFYKPDMD
210 220 230 240 250
SEYPRVDGKL SISCYFRAID NCYNRYAKAF EKKYGKSFSL DQVDFALFHS
260 270 280 290 300
PYNKLVQKSF GRMLYNDFLN NPNDSRYASL EAYKNVKPED TYFDSVLEKA
310 320 330 340 350
LSAITKNDYA TKVAPTTLLA KQLGNTYCGS TYSGLLSLLD EKSNDLVGKR
360 370 380 390 400
VLTFSYGSGL AASAFSFKVE KPINHIVEKV DLKNRLAKRV RVEPEIFTEK
410 420 430 440 450
LSLRETRHNL KNYVPSDETT NMFPGSFYLS SVDNAGIRKY DRTYSTSAVL
460 470 480
GAFQRRQQIS QSTIKSLNLF RATKSVLSIL KK
Length:482
Mass (Da):53,741
Last modified:December 4, 2007 - v2
Checksum:iE6A00A6064B765AF
GO

Sequence cautioni

The sequence AAA91055 differs from that shown. Reason: Frameshift at positions 461 and 482.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti304 – 306ITK → RHE in AAA91055 (Ref. 3) Curated3
Sequence conflicti434N → K in AAA91055 (Ref. 3) Curated1
Sequence conflicti440Y → F in AAA91055 (Ref. 3) Curated1
Sequence conflicti461 – 466QSTIKS → HQQSNL in AAA91055 (Ref. 3) Curated6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000111 Genomic DNA. Translation: EAL63202.1.
L24114 mRNA. Translation: AAA91055.1. Frameshift.
RefSeqiXP_636713.1. XM_631621.1.

Genome annotation databases

EnsemblProtistsiEAL63202; EAL63202; DDB_G0288461.
GeneIDi8626645.
KEGGiddi:DDB_G0288461.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000111 Genomic DNA. Translation: EAL63202.1.
L24114 mRNA. Translation: AAA91055.1. Frameshift.
RefSeqiXP_636713.1. XM_631621.1.

3D structure databases

ProteinModelPortaliP54872.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0219924.

Proteomic databases

PaxDbiP54872.
PRIDEiP54872.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL63202; EAL63202; DDB_G0288461.
GeneIDi8626645.
KEGGiddi:DDB_G0288461.

Organism-specific databases

dictyBaseiDDB_G0288461. hgsA.

Phylogenomic databases

eggNOGiKOG1393. Eukaryota.
COG3425. LUCA.
InParanoidiP54872.
KOiK01641.
OMAiQRCISEE.
PhylomeDBiP54872.

Enzyme and pathway databases

UniPathwayiUPA00058; UER00102.
ReactomeiR-DDI-191273. Cholesterol biosynthesis.
R-DDI-77111. Synthesis of Ketone Bodies.

Miscellaneous databases

PROiP54872.

Family and domain databases

Gene3Di3.40.47.10. 1 hit.
InterProiIPR013746. HMG_CoA_synt_C_dom.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
[Graphical view]
PfamiPF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF53901. SSF53901. 3 hits.
TIGRFAMsiTIGR01833. HMG-CoA-S_euk. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHMCSA_DICDI
AccessioniPrimary (citable) accession number: P54872
Secondary accession number(s): Q54IW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 4, 2007
Last modified: November 2, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.