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P54872 (HMCSA_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxymethylglutaryl-CoA synthase A

Short name=HMG-CoA synthase A
EC=2.3.3.10
Alternative name(s):
3-hydroxy-3-methylglutaryl coenzyme A synthase A
Gene names
Name:hgsA
ORF Names:DDB_G0288461
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length482 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase By similarity.

Catalytic activity

Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.

Pathway

Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.

Sequence similarities

Belongs to the HMG-CoA synthase family.

Sequence caution

The sequence AAA91055.1 differs from that shown. Reason: Frameshift at positions 461 and 482.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 482482Hydroxymethylglutaryl-CoA synthase A
PRO_0000213755

Experimental info

Sequence conflict304 – 3063ITK → RHE in AAA91055. Ref.3
Sequence conflict4341N → K in AAA91055. Ref.3
Sequence conflict4401Y → F in AAA91055. Ref.3
Sequence conflict461 – 4666QSTIKS → HQQSNL in AAA91055. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P54872 [UniParc].

Last modified December 4, 2007. Version 2.
Checksum: E6A00A6064B765AF

FASTA48253,741
        10         20         30         40         50         60 
MTKPENIGIH GIEVYFPSTY VAQEDLEKFD GVSQGKYTLG LGQTNMAFCG DREDIYSLSL 

        70         80         90        100        110        120 
NAVNNLMDKF NVDPNSIGRL EVGTETVIDK SKSVKTVLMD LFAKHGNTSI DGIDTINACY 

       130        140        150        160        170        180 
GGTSALHNAL QWMESSYWDG RNAIVVAGDI AVYEKGPARP TGGAGVVAML IGPNAPITFE 

       190        200        210        220        230        240 
SGLRGVHMEN VYDFYKPDMD SEYPRVDGKL SISCYFRAID NCYNRYAKAF EKKYGKSFSL 

       250        260        270        280        290        300 
DQVDFALFHS PYNKLVQKSF GRMLYNDFLN NPNDSRYASL EAYKNVKPED TYFDSVLEKA 

       310        320        330        340        350        360 
LSAITKNDYA TKVAPTTLLA KQLGNTYCGS TYSGLLSLLD EKSNDLVGKR VLTFSYGSGL 

       370        380        390        400        410        420 
AASAFSFKVE KPINHIVEKV DLKNRLAKRV RVEPEIFTEK LSLRETRHNL KNYVPSDETT 

       430        440        450        460        470        480 
NMFPGSFYLS SVDNAGIRKY DRTYSTSAVL GAFQRRQQIS QSTIKSLNLF RATKSVLSIL 


KK 

« Hide

References

« Hide 'large scale' references
[1]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]Bienvenut W.V., Ura S., Insall R.H.
Submitted (JUL-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 96-104; 263-276; 300-321 AND 390-400, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: AX2.
[3]"A putative partial cDNA clone of HMG-CoA synthase from Dictyostelium discoideum."
Dhar M.S., Hauser L.J., Olins D.E., Olins A.L.
Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 304-482.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000111 Genomic DNA. Translation: EAL63202.1.
L24114 mRNA. Translation: AAA91055.1. Frameshift.

3D structure databases

ProteinModelPortalP54872.
SMRP54872. Positions 3-443.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0219924.

Proteomic databases

PRIDEP54872.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0219924; DDB0219924; DDB_G0288461.
KEGGddi:DDB_G0288461.

Organism-specific databases

dictyBaseDDB_G0288461. hgsA.

Phylogenomic databases

eggNOGCOG3425.
KOK01641.
OMAVPTCKLV.
PhylomeDBP54872.
ProtClustDBCLSZ2429883.

Enzyme and pathway databases

UniPathwayUPA00058; UER00102.

Family and domain databases

Gene3D3.40.47.10. 1 hit.
InterProIPR013746. HMG_CoA_synt_C.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamPF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view]
SUPFAMSSF53901. SSF53901. 3 hits.
TIGRFAMsTIGR01833. HMG-CoA-S_euk. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHMCSA_DICDI
AccessionPrimary (citable) accession number: P54872
Secondary accession number(s): Q54IW3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 4, 2007
Last modified: April 16, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase