##gff-version 3
P54871	UniProtKB	Chain	1	462	.	.	.	ID=PRO_0000213754;Note=Hydroxymethylglutaryl-CoA synthase	
P54871	UniProtKB	Active site	92	92	.	.	.	Note=Proton donor/acceptor;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU10116	
P54871	UniProtKB	Active site	124	124	.	.	.	Note=Acyl-thioester intermediate;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU10116	
P54871	UniProtKB	Active site	257	257	.	.	.	Note=Proton donor/acceptor;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU10116	
P54871	UniProtKB	Cross-link	408	408	.	.	.	Note=Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO);Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:25187565;Dbxref=PMID:25187565	
P54871	UniProtKB	Mutagenesis	291	291	.	.	.	Note=Reduced sumoylation%3B when associated with R-314%2C R-408 and R-420. K->R;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:25187565;Dbxref=PMID:25187565	
P54871	UniProtKB	Mutagenesis	314	314	.	.	.	Note=Reduced sumoylation%3B when associated with R-291%2C R-408 and R-420. K->R;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:25187565;Dbxref=PMID:25187565	
P54871	UniProtKB	Mutagenesis	408	408	.	.	.	Note=Reduced sumoylation. K->R;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:25187565;Dbxref=PMID:25187565	
P54871	UniProtKB	Mutagenesis	420	420	.	.	.	Note=Reduced sumoylation%3B when associated with R-291%2C R-314 and R-408. K->R;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:25187565;Dbxref=PMID:25187565