Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Hydroxymethylglutaryl-CoA synthase

Gene

hmgs-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.By similarity

Catalytic activityi

Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.PROSITE-ProRule annotation

Pathwayi: (R)-mevalonate biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes (R)-mevalonate from acetyl-CoA.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Hydroxymethylglutaryl-CoA synthase (hmgs-1)
  3. 3-hydroxy-3-methylglutaryl coenzyme A reductase (hmgr-1)
This subpathway is part of the pathway (R)-mevalonate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-mevalonate from acetyl-CoA, the pathway (R)-mevalonate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei92Proton donor/acceptorPROSITE-ProRule annotation1
Active sitei124Acyl-thioester intermediatePROSITE-ProRule annotation1
Active sitei257Proton donor/acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Enzyme and pathway databases

BRENDAi2.3.3.10. 1045.
ReactomeiR-CEL-191273. Cholesterol biosynthesis.
R-CEL-77111. Synthesis of Ketone Bodies.
UniPathwayiUPA00058; UER00102.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxymethylglutaryl-CoA synthase (EC:2.3.3.10)
Short name:
HMG-CoA synthase
Alternative name(s):
3-hydroxy-3-methylglutaryl coenzyme A synthase
Gene namesi
Name:hmgs-1Imported
ORF Names:F25B4.6Imported
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiF25B4.6; CE09624; WBGene00017769; hmgs-1.

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown results in sterility, slow development, reduced body size, severe paralysis and reduced pharyngeal pumping.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi291K → R: Reduced sumoylation; when associated with R-314, R-408 and R-420. 1 Publication1
Mutagenesisi314K → R: Reduced sumoylation; when associated with R-291, R-408 and R-420. 1 Publication1
Mutagenesisi408K → R: Reduced sumoylation. 1 Publication1
Mutagenesisi420K → R: Reduced sumoylation; when associated with R-291, R-314 and R-408. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002137541 – 462Hydroxymethylglutaryl-CoA synthaseAdd BLAST462

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki408Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)1 Publication

Post-translational modificationi

Ubiquitinated.1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiP54871.
PaxDbiP54871.
PeptideAtlasiP54871.
PRIDEiP54871.

Expressioni

Gene expression databases

BgeeiWBGene00017769.

Interactioni

Protein-protein interaction databases

BioGridi44005. 1 interactor.
IntActiP54871. 1 interactor.
STRINGi6239.F25B4.6.

Structurei

3D structure databases

ProteinModelPortaliP54871.
SMRiP54871.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HMG-CoA synthase family.Curated

Phylogenomic databases

eggNOGiKOG1393. Eukaryota.
COG3425. LUCA.
GeneTreeiENSGT00390000006096.
HOGENOMiHOG000012351.
InParanoidiP54871.
KOiK01641.
OMAiQWKQAGS.
OrthoDBiEOG091G0791.
PhylomeDBiP54871.

Family and domain databases

Gene3Di3.40.47.10. 1 hit.
InterProiIPR000590. HMG_CoA_synt_AS.
IPR013746. HMG_CoA_synt_C_dom.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
[Graphical view]
PfamiPF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF53901. SSF53901. 3 hits.
TIGRFAMsiTIGR01833. HMG-CoA-S_euk. 1 hit.
PROSITEiPS01226. HMG_COA_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54871-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLGQLSYTP VTDVGIGAIE LYFPQNFVDQ NDLEKFNNVS SGKYTIGLGQ
60 70 80 90 100
QQMGFCSDNE DIVSISLTVT RKLIETYKIS TDSIGCLVVG TETMIDKSKS
110 120 130 140 150
VKTALMDLFP GNSDIEGVDI KNACFGGAQA LLHAIDWVTV NHPLDKKNAI
160 170 180 190 200
VVVADIAIYE EGPARCTGGA GAIAFLICPD ASIPIDRQFS ACHMKNTWDF
210 220 230 240 250
FKPITPIPSE YPVVDGSLSL SSYLEAVRMT YTYFISKVNR HTTGIDGLNS
260 270 280 290 300
FDGVFLHSPF TKMVQKGLAV MNYTDSQLRH KQLNGNGVDH KLDENDRAGL
310 320 330 340 350
AKMIELSAQV WKEKTDPYLV FNRRIGNMYT PSLFAQLLAY LAADDCVTGE
360 370 380 390 400
KSILFFAYGS GLASAIFPGR VRQTSNLDKI RQVAIRAIKR LDDRIQFTPE
410 420 430 440 450
EFTETLQKRE VFLRSKEIPK SPSETSLFPN TYFLDNMDKL YRRSYTLHEE
460
PNGVQNGNGI HH
Length:462
Mass (Da):51,416
Last modified:June 20, 2002 - v2
Checksum:i89BDE382588F6D9F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080538 Genomic DNA. Translation: CCD64508.1.
U12787 mRNA. Translation: AAA92672.1.
PIRiT25726.
RefSeqiNP_504496.1. NM_072095.3.
UniGeneiCel.3914.

Genome annotation databases

EnsemblMetazoaiF25B4.6; F25B4.6; WBGene00017769.
GeneIDi178956.
KEGGicel:CELE_F25B4.6.
UCSCiF25B4.6. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080538 Genomic DNA. Translation: CCD64508.1.
U12787 mRNA. Translation: AAA92672.1.
PIRiT25726.
RefSeqiNP_504496.1. NM_072095.3.
UniGeneiCel.3914.

3D structure databases

ProteinModelPortaliP54871.
SMRiP54871.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi44005. 1 interactor.
IntActiP54871. 1 interactor.
STRINGi6239.F25B4.6.

Proteomic databases

EPDiP54871.
PaxDbiP54871.
PeptideAtlasiP54871.
PRIDEiP54871.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF25B4.6; F25B4.6; WBGene00017769.
GeneIDi178956.
KEGGicel:CELE_F25B4.6.
UCSCiF25B4.6. c. elegans.

Organism-specific databases

CTDi178956.
WormBaseiF25B4.6; CE09624; WBGene00017769; hmgs-1.

Phylogenomic databases

eggNOGiKOG1393. Eukaryota.
COG3425. LUCA.
GeneTreeiENSGT00390000006096.
HOGENOMiHOG000012351.
InParanoidiP54871.
KOiK01641.
OMAiQWKQAGS.
OrthoDBiEOG091G0791.
PhylomeDBiP54871.

Enzyme and pathway databases

UniPathwayiUPA00058; UER00102.
BRENDAi2.3.3.10. 1045.
ReactomeiR-CEL-191273. Cholesterol biosynthesis.
R-CEL-77111. Synthesis of Ketone Bodies.

Miscellaneous databases

PROiP54871.

Gene expression databases

BgeeiWBGene00017769.

Family and domain databases

Gene3Di3.40.47.10. 1 hit.
InterProiIPR000590. HMG_CoA_synt_AS.
IPR013746. HMG_CoA_synt_C_dom.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
[Graphical view]
PfamiPF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF53901. SSF53901. 3 hits.
TIGRFAMsiTIGR01833. HMG-CoA-S_euk. 1 hit.
PROSITEiPS01226. HMG_COA_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHMCS_CAEEL
AccessioniPrimary (citable) accession number: P54871
Secondary accession number(s): Q22962
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: June 20, 2002
Last modified: November 2, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.