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Protein

Hydroxymethylglutaryl-CoA synthase

Gene

F25B4.6

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.By similarity

Catalytic activityi

Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei92 – 921Proton donor/acceptorPROSITE-ProRule annotation
Active sitei124 – 1241Acyl-thioester intermediatePROSITE-ProRule annotation
Active sitei257 – 2571Proton donor/acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. hydroxymethylglutaryl-CoA synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. isoprenoid biosynthetic process Source: InterPro
  2. sterol biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Enzyme and pathway databases

BRENDAi2.3.3.10. 1045.
ReactomeiREACT_276918. Activation of gene expression by SREBF (SREBP).
REACT_304599. PPARA activates gene expression.
REACT_310269. Cholesterol biosynthesis.
REACT_343709. Synthesis of Ketone Bodies.
UniPathwayiUPA00058; UER00102.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxymethylglutaryl-CoA synthase (EC:2.3.3.10)
Short name:
HMG-CoA synthase
Alternative name(s):
3-hydroxy-3-methylglutaryl coenzyme A synthase
Gene namesi
ORF Names:F25B4.6
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiF25B4.6; CE09624; WBGene00017769.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 462462Hydroxymethylglutaryl-CoA synthasePRO_0000213754Add
BLAST

Proteomic databases

PaxDbiP54871.
PRIDEiP54871.

Interactioni

Protein-protein interaction databases

BioGridi44005. 1 interaction.
IntActiP54871. 1 interaction.
STRINGi6239.F25B4.6.

Structurei

3D structure databases

ProteinModelPortaliP54871.
SMRiP54871. Positions 13-445.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HMG-CoA synthase family.Curated

Phylogenomic databases

eggNOGiCOG3425.
GeneTreeiENSGT00390000006096.
HOGENOMiHOG000012351.
InParanoidiP54871.
KOiK01641.
OMAiYERIFFE.
OrthoDBiEOG741Z1W.
PhylomeDBiP54871.

Family and domain databases

Gene3Di3.40.47.10. 1 hit.
InterProiIPR000590. HMG_CoA_synt_AS.
IPR013746. HMG_CoA_synt_C_dom.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF53901. SSF53901. 3 hits.
TIGRFAMsiTIGR01833. HMG-CoA-S_euk. 1 hit.
PROSITEiPS01226. HMG_COA_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54871-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLGQLSYTP VTDVGIGAIE LYFPQNFVDQ NDLEKFNNVS SGKYTIGLGQ
60 70 80 90 100
QQMGFCSDNE DIVSISLTVT RKLIETYKIS TDSIGCLVVG TETMIDKSKS
110 120 130 140 150
VKTALMDLFP GNSDIEGVDI KNACFGGAQA LLHAIDWVTV NHPLDKKNAI
160 170 180 190 200
VVVADIAIYE EGPARCTGGA GAIAFLICPD ASIPIDRQFS ACHMKNTWDF
210 220 230 240 250
FKPITPIPSE YPVVDGSLSL SSYLEAVRMT YTYFISKVNR HTTGIDGLNS
260 270 280 290 300
FDGVFLHSPF TKMVQKGLAV MNYTDSQLRH KQLNGNGVDH KLDENDRAGL
310 320 330 340 350
AKMIELSAQV WKEKTDPYLV FNRRIGNMYT PSLFAQLLAY LAADDCVTGE
360 370 380 390 400
KSILFFAYGS GLASAIFPGR VRQTSNLDKI RQVAIRAIKR LDDRIQFTPE
410 420 430 440 450
EFTETLQKRE VFLRSKEIPK SPSETSLFPN TYFLDNMDKL YRRSYTLHEE
460
PNGVQNGNGI HH
Length:462
Mass (Da):51,416
Last modified:June 20, 2002 - v2
Checksum:i89BDE382588F6D9F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080538 Genomic DNA. Translation: CCD64508.1.
U12787 mRNA. Translation: AAA92672.1.
PIRiT25726.
RefSeqiNP_504496.1. NM_072095.3.
UniGeneiCel.3914.

Genome annotation databases

EnsemblMetazoaiF25B4.6; F25B4.6; WBGene00017769.
GeneIDi178956.
KEGGicel:CELE_F25B4.6.
UCSCiF25B4.6. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080538 Genomic DNA. Translation: CCD64508.1.
U12787 mRNA. Translation: AAA92672.1.
PIRiT25726.
RefSeqiNP_504496.1. NM_072095.3.
UniGeneiCel.3914.

3D structure databases

ProteinModelPortaliP54871.
SMRiP54871. Positions 13-445.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi44005. 1 interaction.
IntActiP54871. 1 interaction.
STRINGi6239.F25B4.6.

Proteomic databases

PaxDbiP54871.
PRIDEiP54871.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF25B4.6; F25B4.6; WBGene00017769.
GeneIDi178956.
KEGGicel:CELE_F25B4.6.
UCSCiF25B4.6. c. elegans.

Organism-specific databases

CTDi178956.
WormBaseiF25B4.6; CE09624; WBGene00017769.

Phylogenomic databases

eggNOGiCOG3425.
GeneTreeiENSGT00390000006096.
HOGENOMiHOG000012351.
InParanoidiP54871.
KOiK01641.
OMAiYERIFFE.
OrthoDBiEOG741Z1W.
PhylomeDBiP54871.

Enzyme and pathway databases

UniPathwayiUPA00058; UER00102.
BRENDAi2.3.3.10. 1045.
ReactomeiREACT_276918. Activation of gene expression by SREBF (SREBP).
REACT_304599. PPARA activates gene expression.
REACT_310269. Cholesterol biosynthesis.
REACT_343709. Synthesis of Ketone Bodies.

Miscellaneous databases

NextBioi903278.
PROiP54871.

Family and domain databases

Gene3Di3.40.47.10. 1 hit.
InterProiIPR000590. HMG_CoA_synt_AS.
IPR013746. HMG_CoA_synt_C_dom.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF53901. SSF53901. 3 hits.
TIGRFAMsiTIGR01833. HMG-CoA-S_euk. 1 hit.
PROSITEiPS01226. HMG_COA_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. "Human mitochondrial HMG CoA synthase: liver cDNA and partial genomic cloning, chromosome mapping to 1p12-p13, and possible role in vertebrate evolution."
    Boukaftane Y., Duncan A., Wang S., Labuda D., Robert M.-F., Sarrazin J., Schappert K.T., Mitchell G.A.
    Genomics 23:552-559(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-462.

Entry informationi

Entry nameiHMCS_CAEEL
AccessioniPrimary (citable) accession number: P54871
Secondary accession number(s): Q22962
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: June 20, 2002
Last modified: April 29, 2015
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.