P54871 (HMCS_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxymethylglutaryl-CoA synthase
Short name=HMG-CoA synthase
EC=2.3.3.10

Alternative name(s):
3-hydroxy-3-methylglutaryl coenzyme A synthase

Gene names

ORF Names:

F25B4.6

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase By similarity.
Catalytic activity	Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.
Sequence similarities	Belongs to the HMG-CoA synthase family.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Steroid biosynthesis Steroid metabolism Sterol biosynthesis Sterol metabolism
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: InterPro sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	hydroxymethylglutaryl-CoA synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 462	462	Hydroxymethylglutaryl-CoA synthase		PRO_0000213754
Sites
	Active site	124	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	P54871 [UniParc]. Last modified June 20, 2002. Version 2. Checksum: 89BDE382588F6D9F	FASTA	462	51,416
	10 20 30 40 50 60 MSLGQLSYTP VTDVGIGAIE LYFPQNFVDQ NDLEKFNNVS SGKYTIGLGQ QQMGFCSDNE 70 80 90 100 110 120 DIVSISLTVT RKLIETYKIS TDSIGCLVVG TETMIDKSKS VKTALMDLFP GNSDIEGVDI 130 140 150 160 170 180 KNACFGGAQA LLHAIDWVTV NHPLDKKNAI VVVADIAIYE EGPARCTGGA GAIAFLICPD 190 200 210 220 230 240 ASIPIDRQFS ACHMKNTWDF FKPITPIPSE YPVVDGSLSL SSYLEAVRMT YTYFISKVNR 250 260 270 280 290 300 HTTGIDGLNS FDGVFLHSPF TKMVQKGLAV MNYTDSQLRH KQLNGNGVDH KLDENDRAGL 310 320 330 340 350 360 AKMIELSAQV WKEKTDPYLV FNRRIGNMYT PSLFAQLLAY LAADDCVTGE KSILFFAYGS 370 380 390 400 410 420 GLASAIFPGR VRQTSNLDKI RQVAIRAIKR LDDRIQFTPE EFTETLQKRE VFLRSKEIPK 430 440 450 460 SPSETSLFPN TYFLDNMDKL YRRSYTLHEE PNGVQNGNGI HH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Human mitochondrial HMG CoA synthase: liver cDNA and partial genomic cloning, chromosome mapping to 1p12-p13, and possible role in vertebrate evolution." Boukaftane Y., Duncan A., Wang S., Labuda D., Robert M.-F., Sarrazin J., Schappert K.T., Mitchell G.A. Genomics 23:552-559(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-462.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FO080538 Genomic DNA. Translation: CCD64508.1. U12787 mRNA. Translation: AAA92672.1.
PIR	T25726.
RefSeq	NP_504496.1. NM_072095.3.
3D structure databases
ProteinModelPortal	P54871.
SMR	P54871. Positions 13-445.
ModBase	Search...
Protein-protein interaction databases
IntAct	P54871. 1 interaction.
STRING	6239.F25B4.6.
Proteomic databases
PaxDb	P54871.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	F25B4.6; F25B4.6; F25B4.6.
GeneID	178956.
KEGG	cel:CELE_F25B4.6.
UCSC	F25B4.6. c. elegans.
Organism-specific databases
CTD	178956.
WormBase	F25B4.6; CE09624; WBGene00017769.
Phylogenomic databases
eggNOG	COG3425.
GeneTree	ENSGT00390000006096.
HOGENOM	HOG000012351.
InParanoid	P54871.
KO	K01641.
OMA	DSVTMAS.
Enzyme and pathway databases
BRENDA	2.3.3.10. 1045.
UniPathway	UPA00058; UER00102.
Family and domain databases
Gene3D	3.40.47.10. 1 hit.
InterPro	IPR000590. HMG_CoA_synt_AS. IPR013746. HMG_CoA_synt_C. IPR013528. HMG_CoA_synth_N. IPR010122. HMG_CoA_synthase_euk. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Pfam	PF08540. HMG_CoA_synt_C. 1 hit. PF01154. HMG_CoA_synt_N. 1 hit. [Graphical view]
SUPFAM	SSF53901. Thiolase-like. 2 hits.
TIGRFAMs	TIGR01833. HMG-CoA-S_euk. 1 hit.
PROSITE	PS01226. HMG_COA_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	903278.

Entry information

Entry name

HMCS_CAEEL

Accession

Primary (citable) accession number: P54871
Secondary accession number(s): Q22962

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	June 20, 2002
Last modified:	May 1, 2013
This is version 104 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project