Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Hydroxymethylglutaryl-CoA synthase 2

Gene

HMGCS-2

Organism
Blattella germanica (German cockroach) (Blatta germanica)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.By similarity

Catalytic activityi

Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.PROSITE-ProRule annotation

Pathway: (R)-mevalonate biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes (R)-mevalonate from acetyl-CoA.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Hydroxymethylglutaryl-CoA synthase 1 (HMGCS-1), Hydroxymethylglutaryl-CoA synthase 2 (HMGCS-2)
  3. 3-hydroxy-3-methylglutaryl-coenzyme A reductase
This subpathway is part of the pathway (R)-mevalonate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-mevalonate from acetyl-CoA, the pathway (R)-mevalonate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei86 – 861Proton donor/acceptorPROSITE-ProRule annotation
Active sitei120 – 1201Acyl-thioester intermediatePROSITE-ProRule annotation
Active sitei255 – 2551Proton donor/acceptorPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Enzyme and pathway databases

BRENDAi2.3.3.10. 875.
UniPathwayiUPA00058; UER00102.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxymethylglutaryl-CoA synthase 2 (EC:2.3.3.10)
Short name:
HMG-CoA synthase 2
Alternative name(s):
3-hydroxy-3-methylglutaryl coenzyme A synthase 2
Gene namesi
Name:HMGCS-2
OrganismiBlattella germanica (German cockroach) (Blatta germanica)
Taxonomic identifieri6973 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraOrthopteroideaDictyopteraBlattodeaBlaberoideaEctobiidaeBlattellinaeBlattella

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 455455Hydroxymethylglutaryl-CoA synthase 2PRO_0000213753Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP54870.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HMG-CoA synthase family.Curated

Family and domain databases

Gene3Di3.40.47.10. 1 hit.
InterProiIPR000590. HMG_CoA_synt_AS.
IPR013746. HMG_CoA_synt_C_dom.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF53901. SSF53901. 3 hits.
TIGRFAMsiTIGR01833. HMG-CoA-S_euk. 1 hit.
PROSITEiPS01226. HMG_COA_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54870-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHWPEDVGI IGIEMIFPSL YVDQAELETY DEVSPGKYTM GLGQDKMGVC
60 70 80 90 100
TDREDINSLC LTAVDKLMER NNIDYNDIGW LEVGTETILD KVKSVKTVLM
110 120 130 140 150
QLFEESGNTD VEGIDTINAC YRGTAALFNA LIWIESSSWD GRYAIVVAAD
160 170 180 190 200
IAIYAKECSP TGGAGALLML IGANAPIVID RGVRASHMKH AYDFYKPDLM
210 220 230 240 250
SEYPVVDGKL SVQCYLSALD HCYPRFCSKT EKYLKRCGKE NTKIDLDYFD
260 270 280 290 300
AFVFHSPYCK LVQKSVARLV LNDFIQYPEK YQDLQQLRNL KFEDTYFDRD
310 320 330 340 350
IEKIFMDKSK QLFEKKTKPS LMLANQVGNM YTTSLYGGLV SLLISEDIGE
360 370 380 390 400
LAGKCICMFS YGSGFAASMF SLHISTDSSP GSTLSRLVTN LTHIKPQVQQ
410 420 430 440 450
RVKLSPGEFE NIMEIREQNH HKAPYTPVAS PNTLFPGTWY LESIDSMHRR

KYKRV
Length:455
Mass (Da):51,425
Last modified:October 1, 1996 - v1
Checksum:iDD030356548A63B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77516 mRNA. Translation: CAA54652.1.
PIRiA53565.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77516 mRNA. Translation: CAA54652.1.
PIRiA53565.

3D structure databases

ProteinModelPortaliP54870.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00058; UER00102.
BRENDAi2.3.3.10. 875.

Family and domain databases

Gene3Di3.40.47.10. 1 hit.
InterProiIPR000590. HMG_CoA_synt_AS.
IPR013746. HMG_CoA_synt_C_dom.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF53901. SSF53901. 3 hits.
TIGRFAMsiTIGR01833. HMG-CoA-S_euk. 1 hit.
PROSITEiPS01226. HMG_COA_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Blattella germanica has two HMG-CoA synthase genes. Both are regulated in the ovary during the gonadotrophic cycle."
    Buesa C., Martinez-Gonzalez J., Casals N., Haro D., Piulachs M.D., Belles X., Hegardt F.G.
    J. Biol. Chem. 269:11707-11713(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiHMCS2_BLAGE
AccessioniPrimary (citable) accession number: P54870
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 1, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.