P54855 (UDB15_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-glucuronosyltransferase 2B15
Short name=UDPGT 2B15
EC=2.4.1.17

Alternative name(s):
HLUG4
UDP-glucuronosyltransferase 2B8
Short name=UDPGT 2B8
UDPGTh-3

Gene names

Name:	UGT2B15
Synonyms:	UGT2B8

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	530 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	UDPGTs are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isozyme displays activity toward several classes of xenobiotic substrates, including simple phenolic compounds, 7-hydroxylated coumarins, flavonoids, anthraquinones, and certain drugs and their hydroxylated metabolites. It also catalyzes the glucuronidation of endogenous estrogens and androgens.
Catalytic activity	UDP-glucuronate + acceptor = UDP + acceptor beta-D-glucuronoside.
Subcellular location	Microsome membrane; Single-pass membrane protein Potential. Endoplasmic reticulum membrane; Single-pass membrane protein Potential.
Tissue specificity	Expressed in many tissues. Present in liver, prostate and testis. Ref.1
Sequence similarities	Belongs to the UDP-glycosyltransferase family.
Caution	Ref.6 thought that this was a separate form (UGT2B8). The name UGT2B8 has now been reused for a rat enzyme.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Coding sequence diversity	Polymorphism
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cellular glucuronidation Inferred from direct assay PubMed 20056724. Source: BHF-UCL steroid metabolic process Traceable author statement Ref.2. Source: ProtInc xenobiotic metabolic process Traceable author statement Ref.2. Source: ProtInc
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	glucuronosyltransferase activity Inferred from direct assay PubMed 20056724. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 530	507	UDP-glucuronosyltransferase 2B15		PRO_0000036039
Regions
	Transmembrane	495 – 515	21	Helical; Potential
Amino acid modifications
	Glycosylation	65	1	N-linked (GlcNAc...) Ref.7
	Glycosylation	316	1	N-linked (GlcNAc...) Potential
	Glycosylation	483	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	85	1	Y → D. Ref.1 Ref.2 Corresponds to variant rs1902023 [ dbSNP | Ensembl ].		VAR_007713
	Natural variant	523	1	K → T. Ref.1 Ref.2 Ref.3 Ref.8 Corresponds to variant rs4148269 [ dbSNP | Ensembl ].		VAR_018348
Experimental info
	Sequence conflict	119	1	E → A in AAA83406. Ref.6
	Sequence conflict	145	1	K → R in AAA83406. Ref.6
	Sequence conflict	150 – 155	6	LADALN → PGDPVF in AAA83406. Ref.6
	Sequence conflict	162	1	A → S in AAA83406. Ref.6
	Sequence conflict	165	1	F → L in AAA83406. Ref.6
	Sequence conflict	170 – 177	8	LYSLRFSV → VYRSRISR in AAA83406. Ref.6
	Sequence conflict	181	1	F → I in AAA83406. Ref.6
	Sequence conflict	203	1	S → I in AAA83406. Ref.6
	Sequence conflict	293	1	M → L in AAA83406. Ref.6
	Sequence conflict	401	1	H → D in AAA83406. Ref.6
	Sequence conflict	443	1	V → A in AAA83406. Ref.6
	Sequence conflict	501	1	C → W in AAA83406. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P54855 [UniParc]. Last modified May 3, 2011. Version 3. Checksum: EB2DAE838769C955	FASTA	530	61,036
	10 20 30 40 50 60 MSLKWTSVFL LIQLSCYFSS GSCGKVLVWP TEYSHWINMK TILEELVQRG HEVTVLTSSA 70 80 90 100 110 120 STLVNASKSS AIKLEVYPTS LTKNYLEDSL LKILDRWIYG VSKNTFWSYF SQLQELCWEY 130 140 150 160 170 180 YDYSNKLCKD AVLNKKLMMK LQESKFDVIL ADALNPCGEL LAELFNIPFL YSLRFSVGYT 190 200 210 220 230 240 FEKNGGGFLF PPSYVPVVMS ELSDQMIFME RIKNMIHMLY FDFWFQIYDL KKWDQFYSEV 250 260 270 280 290 300 LGRPTTLFET MGKAEMWLIR TYWDFEFPRP FLPNVDFVGG LHCKPAKPLP KEMEEFVQSS 310 320 330 340 350 360 GENGIVVFSL GSMISNMSEE SANMIASALA QIPQKVLWRF DGKKPNTLGS NTRLYKWLPQ 370 380 390 400 410 420 NDLLGHPKTK AFITHGGTNG IYEAIYHGIP MVGIPLFADQ HDNIAHMKAK GAALSVDIRT 430 440 450 460 470 480 MSSRDLLNAL KSVINDPVYK ENVMKLSRIH HDQPMKPLDR AVFWIEFVMR HKGAKHLRVA 490 500 510 520 530 AHNLTWIQYH SLDVIAFLLA CVATVIFIIT KFCLFCFRKL AKKGKKKKRD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a cloned human dihydrotestosterone/androstanediol UDP-glucuronosyltransferase and its comparison to other steroid isoforms." Chen F., Ritter J.K., Wang M.G., McBride O.W., Lubet R.A., Owens I.S. Biochemistry 32:10648-10657(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, TISSUE SPECIFICITY, VARIANTS ASP-85 AND THR-523. Tissue: Liver.
[2]	"Stable expression of a human liver UDP-glucuronosyltransferase (UGT2B15) with activity toward steroid and xenobiotic substrates." Green M.D., Oturu E.M., Tephly T.R. Drug Metab. Dispos. 22:799-805(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS ASP-85 AND THR-523. Tissue: Liver.
[3]	"Isolation and characterization of UGT2B15(Y85): a UDP-glucuronosyltransferase encoded by a polymorphic gene." Levesque E., Beaulieu M., Green M.D., Tephly T.R., Belanger A., Hum D.W. Pharmacogenetics 7:317-325(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT THR-523.
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"Characterization and primary sequence of a human hepatic microsomal estriol UDPglucuronosyltransferase." Coffman B.L., Tephly T.R., Irshaid Y.M., Green M.D., Smith C., Jackson M.R., Wooster R., Burchell B. Arch. Biochem. Biophys. 281:170-175(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-530, PARTIAL PROTEIN SEQUENCE. Tissue: Liver.
[7]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-65, MASS SPECTROMETRY. Tissue: Liver.
[8]	"A major genotype in UDP-glucuronosyltransferase 2B15." Toide K., Umeda S., Yamazaki H., Takahashi Y., Terauchi Y., Fujii T., Kamataki T. Drug Metab. Pharmacokinet. 17:164-166(2002) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT THR-523.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF548389 mRNA. Translation: AAN40695.1. U08854 mRNA. Translation: AAC50077.1. AF180322 mRNA. Translation: AAD55093.1. AC019173 Genomic DNA. No translation available. AC147055 Genomic DNA. No translation available. CH471057 Genomic DNA. Translation: EAX05574.1. U06641 mRNA. Translation: AAA83406.1.
IPI	IPI00008905.
PIR	A48633. S11309.
RefSeq	NP_001067.2. NM_001076.3.
UniGene	Hs.150207.
3D structure databases
ProteinModelPortal	P54855.
SMR	P54855. Positions 286-447.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000341045.
Protein family/group databases
CAZy	GT1. Glycosyltransferase Family 1.
PTM databases
PhosphoSite	P54855.
Polymorphism databases
DMDM	143811472.
Proteomic databases
PaxDb	P54855.
PRIDE	P54855.
Protocols and materials databases
DNASU	7366.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000338206; ENSP00000341045; ENSG00000196620.
GeneID	7366.
KEGG	hsa:7366.
UCSC	uc021xow.1. human.
Organism-specific databases
CTD	7366.
GeneCards	GC04M069561.
H-InvDB	HIX0031375. HIX0164240.
HGNC	HGNC:12546. UGT2B15.
HPA	HPA045108.
MIM	600069. gene.
neXtProt	NX_P54855.
PharmGKB	PA37188.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1819.
HOGENOM	HOG000220831.
HOVERGEN	HBG004033.
InParanoid	P54855.
KO	K00699.
OMA	WEYSDCI.
OrthoDB	EOG4DV5KX.
PhylomeDB	P54855.
Enzyme and pathway databases
BRENDA	2.4.1.17. 2681.
Reactome	REACT_111217. Metabolism.
SABIO-RK	P54855.
Gene expression databases
Bgee	P54855.
CleanEx	HS_UGT2B15.
Genevestigator	P54855.
GermOnline	ENSG00000197592. Homo sapiens.
Family and domain databases
InterPro	IPR002213. UDP_glucos_trans. [Graphical view]
PANTHER	PTHR11926. PTHR11926. 1 hit.
Pfam	PF00201. UDPGT. 1 hit. [Graphical view]
PROSITE	PS00375. UDPGT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL6161.
GenomeRNAi	7366.
NextBio	28842.
SOURCE	Search...

Entry information

Entry name

UDB15_HUMAN

Accession

Primary (citable) accession number: P54855
Secondary accession number(s): A6NDX0 , A6NNJ4, P23765, Q9UK63

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	May 3, 2011
Last modified:	April 3, 2013
This is version 120 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.