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Protein

UDP-glucuronosyltransferase 2B15

Gene

UGT2B15

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

UDPGTs are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isozyme displays activity toward several classes of xenobiotic substrates, including simple phenolic compounds, 7-hydroxylated coumarins, flavonoids, anthraquinones, and certain drugs and their hydroxylated metabolites. It also catalyzes the glucuronidation of endogenous estrogens and androgens.

Catalytic activityi

UDP-glucuronate + acceptor = UDP + acceptor beta-D-glucuronoside.

GO - Molecular functioni

  1. glucuronosyltransferase activity Source: UniProtKB

GO - Biological processi

  1. cellular glucuronidation Source: UniProtKB
  2. cellular response to hormone stimulus Source: GO_Central
  3. flavonoid biosynthetic process Source: GO_Central
  4. flavonoid glucuronidation Source: GO_Central
  5. steroid metabolic process Source: ProtInc
  6. xenobiotic metabolic process Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.17. 2681.
ReactomeiREACT_6784. Glucuronidation.
SABIO-RKP54855.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-glucuronosyltransferase 2B15 (EC:2.4.1.17)
Short name:
UDPGT 2B15
Alternative name(s):
HLUG4
UDP-glucuronosyltransferase 2B8
Short name:
UDPGT 2B8
UDPGTh-3
Gene namesi
Name:UGT2B15
Synonyms:UGT2B8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 4

Organism-specific databases

HGNCiHGNC:12546. UGT2B15.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei495 – 51521HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. intracellular membrane-bounded organelle Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA37188.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 530507UDP-glucuronosyltransferase 2B15PRO_0000036039Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi65 – 651N-linked (GlcNAc...)1 Publication
Glycosylationi316 – 3161N-linked (GlcNAc...)Sequence Analysis
Glycosylationi483 – 4831N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP54855.
PRIDEiP54855.

PTM databases

PhosphoSiteiP54855.

Expressioni

Tissue specificityi

Expressed in many tissues. Present in liver, prostate and testis.1 Publication

Gene expression databases

BgeeiP54855.
CleanExiHS_UGT2B15.
GenevestigatoriP54855.

Organism-specific databases

HPAiHPA045108.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000341045.

Structurei

3D structure databases

ProteinModelPortaliP54855.
SMRiP54855. Positions 286-447.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the UDP-glycosyltransferase family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1819.
GeneTreeiENSGT00760000118949.
HOGENOMiHOG000220831.
HOVERGENiHBG004033.
InParanoidiP54855.
KOiK00699.
OMAiMPAMMAM.
OrthoDBiEOG7GBFWS.
PhylomeDBiP54855.
TreeFamiTF315472.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEiPS00375. UDPGT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P54855-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLKWTSVFL LIQLSCYFSS GSCGKVLVWP TEYSHWINMK TILEELVQRG
60 70 80 90 100
HEVTVLTSSA STLVNASKSS AIKLEVYPTS LTKNYLEDSL LKILDRWIYG
110 120 130 140 150
VSKNTFWSYF SQLQELCWEY YDYSNKLCKD AVLNKKLMMK LQESKFDVIL
160 170 180 190 200
ADALNPCGEL LAELFNIPFL YSLRFSVGYT FEKNGGGFLF PPSYVPVVMS
210 220 230 240 250
ELSDQMIFME RIKNMIHMLY FDFWFQIYDL KKWDQFYSEV LGRPTTLFET
260 270 280 290 300
MGKAEMWLIR TYWDFEFPRP FLPNVDFVGG LHCKPAKPLP KEMEEFVQSS
310 320 330 340 350
GENGIVVFSL GSMISNMSEE SANMIASALA QIPQKVLWRF DGKKPNTLGS
360 370 380 390 400
NTRLYKWLPQ NDLLGHPKTK AFITHGGTNG IYEAIYHGIP MVGIPLFADQ
410 420 430 440 450
HDNIAHMKAK GAALSVDIRT MSSRDLLNAL KSVINDPVYK ENVMKLSRIH
460 470 480 490 500
HDQPMKPLDR AVFWIEFVMR HKGAKHLRVA AHNLTWIQYH SLDVIAFLLA
510 520 530
CVATVIFIIT KFCLFCFRKL AKKGKKKKRD
Length:530
Mass (Da):61,036
Last modified:May 3, 2011 - v3
Checksum:iEB2DAE838769C955
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti119 – 1191E → A in AAA83406. (PubMed:2116769)Curated
Sequence conflicti145 – 1451K → R in AAA83406. (PubMed:2116769)Curated
Sequence conflicti150 – 1556LADALN → PGDPVF in AAA83406. (PubMed:2116769)Curated
Sequence conflicti162 – 1621A → S in AAA83406. (PubMed:2116769)Curated
Sequence conflicti165 – 1651F → L in AAA83406. (PubMed:2116769)Curated
Sequence conflicti170 – 1778LYSLRFSV → VYRSRISR in AAA83406. (PubMed:2116769)Curated
Sequence conflicti181 – 1811F → I in AAA83406. (PubMed:2116769)Curated
Sequence conflicti203 – 2031S → I in AAA83406. (PubMed:2116769)Curated
Sequence conflicti293 – 2931M → L in AAA83406. (PubMed:2116769)Curated
Sequence conflicti401 – 4011H → D in AAA83406. (PubMed:2116769)Curated
Sequence conflicti443 – 4431V → A in AAA83406. (PubMed:2116769)Curated
Sequence conflicti501 – 5011C → W in AAA83406. (PubMed:2116769)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti85 – 851Y → D.2 Publications
Corresponds to variant rs1902023 [ dbSNP | Ensembl ].
VAR_007713
Natural varianti523 – 5231K → T.5 Publications
Corresponds to variant rs4148269 [ dbSNP | Ensembl ].
VAR_018348

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF548389 mRNA. Translation: AAN40695.1.
U08854 mRNA. Translation: AAC50077.1.
AF180322 mRNA. Translation: AAD55093.1.
AK289419 mRNA. Translation: BAF82108.1.
AC019173 Genomic DNA. No translation available.
AC147055 Genomic DNA. No translation available.
CH471057 Genomic DNA. Translation: EAX05574.1.
U06641 mRNA. Translation: AAA83406.1.
CCDSiCCDS3524.1.
PIRiA48633.
S11309.
RefSeqiNP_001067.2. NM_001076.3.
UniGeneiHs.150207.

Genome annotation databases

EnsembliENST00000338206; ENSP00000341045; ENSG00000196620.
ENST00000616841; ENSP00000482004; ENSG00000196620.
GeneIDi7366.
KEGGihsa:7366.
UCSCiuc021xow.1. human.

Polymorphism databases

DMDMi332278237.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF548389 mRNA. Translation: AAN40695.1.
U08854 mRNA. Translation: AAC50077.1.
AF180322 mRNA. Translation: AAD55093.1.
AK289419 mRNA. Translation: BAF82108.1.
AC019173 Genomic DNA. No translation available.
AC147055 Genomic DNA. No translation available.
CH471057 Genomic DNA. Translation: EAX05574.1.
U06641 mRNA. Translation: AAA83406.1.
CCDSiCCDS3524.1.
PIRiA48633.
S11309.
RefSeqiNP_001067.2. NM_001076.3.
UniGeneiHs.150207.

3D structure databases

ProteinModelPortaliP54855.
SMRiP54855. Positions 286-447.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000341045.

Chemistry

ChEMBLiCHEMBL6161.
DrugBankiDB00316. Acetaminophen.
DB06695. Dabigatran etexilate.
DB00973. Ezetimibe.
DB00186. Lorazepam.
DB00295. Morphine.
DB00842. Oxazepam.
DB00313. Valproic Acid.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

PTM databases

PhosphoSiteiP54855.

Polymorphism databases

DMDMi332278237.

Proteomic databases

PaxDbiP54855.
PRIDEiP54855.

Protocols and materials databases

DNASUi7366.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000338206; ENSP00000341045; ENSG00000196620.
ENST00000616841; ENSP00000482004; ENSG00000196620.
GeneIDi7366.
KEGGihsa:7366.
UCSCiuc021xow.1. human.

Organism-specific databases

CTDi7366.
GeneCardsiGC04M069561.
H-InvDBHIX0031375.
HIX0164240.
HGNCiHGNC:12546. UGT2B15.
HPAiHPA045108.
MIMi600069. gene.
neXtProtiNX_P54855.
PharmGKBiPA37188.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG1819.
GeneTreeiENSGT00760000118949.
HOGENOMiHOG000220831.
HOVERGENiHBG004033.
InParanoidiP54855.
KOiK00699.
OMAiMPAMMAM.
OrthoDBiEOG7GBFWS.
PhylomeDBiP54855.
TreeFamiTF315472.

Enzyme and pathway databases

BRENDAi2.4.1.17. 2681.
ReactomeiREACT_6784. Glucuronidation.
SABIO-RKP54855.

Miscellaneous databases

GeneWikiiUGT2B15.
GenomeRNAii7366.
NextBioi28842.
PROiP54855.
SOURCEiSearch...

Gene expression databases

BgeeiP54855.
CleanExiHS_UGT2B15.
GenevestigatoriP54855.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEiPS00375. UDPGT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a cloned human dihydrotestosterone/androstanediol UDP-glucuronosyltransferase and its comparison to other steroid isoforms."
    Chen F., Ritter J.K., Wang M.G., McBride O.W., Lubet R.A., Owens I.S.
    Biochemistry 32:10648-10657(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, TISSUE SPECIFICITY, VARIANTS ASP-85 AND THR-523.
    Tissue: Liver.
  2. "Stable expression of a human liver UDP-glucuronosyltransferase (UGT2B15) with activity toward steroid and xenobiotic substrates."
    Green M.D., Oturu E.M., Tephly T.R.
    Drug Metab. Dispos. 22:799-805(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS ASP-85 AND THR-523.
    Tissue: Liver.
  3. "Isolation and characterization of UGT2B15(Y85): a UDP-glucuronosyltransferase encoded by a polymorphic gene."
    Levesque E., Beaulieu M., Green M.D., Tephly T.R., Belanger A., Hum D.W.
    Pharmacogenetics 7:317-325(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT THR-523.
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-523.
    Tissue: Mammary gland.
  5. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "Characterization and primary sequence of a human hepatic microsomal estriol UDPglucuronosyltransferase."
    Coffman B.L., Tephly T.R., Irshaid Y.M., Green M.D., Smith C., Jackson M.R., Wooster R., Burchell B.
    Arch. Biochem. Biophys. 281:170-175(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-530, PARTIAL PROTEIN SEQUENCE.
    Tissue: Liver.
  8. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
    Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
    J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-65.
    Tissue: Liver.
  9. Cited for: VARIANT THR-523.

Entry informationi

Entry nameiUDB15_HUMAN
AccessioniPrimary (citable) accession number: P54855
Secondary accession number(s): A6NDX0
, A6NNJ4, A8K054, P23765, Q9UK63
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 3, 2011
Last modified: February 4, 2015
This is version 136 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

PubMed:2116769 thought that this was a separate form (UGT2B8). The name UGT2B8 has now been reused for a rat enzyme.Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.