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Protein

Epithelial membrane protein 1

Gene

EMP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GO - Biological processi

  • bleb assembly Source: UniProtKB
  • cell death Source: UniProtKB
  • cell growth Source: InterPro
  • cell proliferation Source: ProtInc
  • epidermis development Source: ProtInc
  • multicellular organism development Source: ProtInc
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Epithelial membrane protein 1
Short name:
EMP-1
Alternative name(s):
CL-20
Protein B4B
Tumor-associated membrane protein
Gene namesi
Name:EMP1
Synonyms:B4B, TMP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:3333. EMP1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1 – 2121HelicalSequence analysisAdd
BLAST
Transmembranei67 – 8721HelicalSequence analysisAdd
BLAST
Transmembranei95 – 11521HelicalSequence analysisAdd
BLAST
Transmembranei134 – 15421HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: ProtInc
  • membrane Source: ProtInc
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27770.

Polymorphism and mutation databases

BioMutaiEMP1.
DMDMi3041684.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 157157Epithelial membrane protein 1PRO_0000164653Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi43 – 431N-linked (GlcNAc...)Sequence analysis
Glycosylationi46 – 461N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP54849.
PRIDEiP54849.
TopDownProteomicsiP54849-1. [P54849-1]

Expressioni

Gene expression databases

BgeeiP54849.
CleanExiHS_EMP1.
ExpressionAtlasiP54849. baseline and differential.
GenevisibleiP54849. HS.

Organism-specific databases

HPAiHPA056250.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CEP70Q8NHQ13EBI-4319440,EBI-739624
SMIM3Q9BZL33EBI-4319440,EBI-741850
SYNE4Q8N2053EBI-4319440,EBI-7131783

Protein-protein interaction databases

BioGridi108327. 3 interactions.
IntActiP54849. 4 interactions.
STRINGi9606.ENSP00000256951.

Structurei

3D structure databases

ProteinModelPortaliP54849.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PMP-22/EMP/MP20 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IW7W. Eukaryota.
ENOG410Z5WT. LUCA.
GeneTreeiENSGT00510000046328.
HOGENOMiHOG000059542.
HOVERGENiHBG001690.
InParanoidiP54849.
OMAiCWLCILV.
OrthoDBiEOG7F24VG.
PhylomeDBiP54849.
TreeFamiTF330414.

Family and domain databases

InterProiIPR003932. EMP_1.
IPR004031. PMP22/EMP/MP20/Claudin.
IPR004032. PMP22_EMP_MP20.
[Graphical view]
PANTHERiPTHR10671:SF6. PTHR10671:SF6. 1 hit.
PfamiPF00822. PMP22_Claudin. 1 hit.
[Graphical view]
PRINTSiPR01453. EPMEMFAMILY.
PR01454. EPMEMPROT1.
PROSITEiPS01221. PMP22_1. 1 hit.
PS01222. PMP22_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P54849-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLVLLAGIFV VHIATVIMLF VSTIANVWLV SNTVDASVGL WKNCTNISCS
60 70 80 90 100
DSLSYASEDA LKTVQAFMIL SIIFCVIALL VFVFQLFTME KGNRFFLSGA
110 120 130 140 150
TTLVCWLCIL VGVSIYTSHY ANRDGTQYHH GYSYILGWIC FCFSFIIGVL

YLVLRKK
Length:157
Mass (Da):17,563
Last modified:July 15, 1998 - v3
Checksum:iB470B4ADC5C16821
GO
Isoform 2 (identifier: P54849-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     106-157: WLCILVGVSI...GVLYLVLRKK → FIMRIVMERS...ASSSAFSIWS

Note: No experimental confirmation available.
Show »
Length:141
Mass (Da):15,252
Checksum:iFBA7A4754382838C
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti57 – 571S → N.
Corresponds to variant rs34412222 [ dbSNP | Ensembl ].
VAR_050608

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei106 – 15752WLCIL…VLRKK → FIMRIVMERSITTAIPTSWA GSASASASSSAFSIWS in isoform 2. 1 PublicationVSP_056984Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43916 mRNA. Translation: AAC51783.1.
U77085 mRNA. Translation: AAC51207.1.
Z50751 mRNA. Translation: CAA90627.1.
Y07909 mRNA. Translation: CAA69217.1.
AK299388 mRNA. Translation: BAG61373.1.
AK312246 mRNA. Translation: BAG35178.1.
AC022276 Genomic DNA. No translation available.
AC079628 Genomic DNA. No translation available.
CH471094 Genomic DNA. Translation: EAW96304.1.
BC047300 mRNA. Translation: AAH47300.1.
CCDSiCCDS8660.1. [P54849-1]
PIRiG02355.
RefSeqiNP_001414.1. NM_001423.2. [P54849-1]
UniGeneiHs.719042.

Genome annotation databases

EnsembliENST00000256951; ENSP00000256951; ENSG00000134531. [P54849-1]
ENST00000396301; ENSP00000379595; ENSG00000134531. [P54849-2]
GeneIDi2012.
KEGGihsa:2012.
UCSCiuc001rbr.4. human. [P54849-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43916 mRNA. Translation: AAC51783.1.
U77085 mRNA. Translation: AAC51207.1.
Z50751 mRNA. Translation: CAA90627.1.
Y07909 mRNA. Translation: CAA69217.1.
AK299388 mRNA. Translation: BAG61373.1.
AK312246 mRNA. Translation: BAG35178.1.
AC022276 Genomic DNA. No translation available.
AC079628 Genomic DNA. No translation available.
CH471094 Genomic DNA. Translation: EAW96304.1.
BC047300 mRNA. Translation: AAH47300.1.
CCDSiCCDS8660.1. [P54849-1]
PIRiG02355.
RefSeqiNP_001414.1. NM_001423.2. [P54849-1]
UniGeneiHs.719042.

3D structure databases

ProteinModelPortaliP54849.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108327. 3 interactions.
IntActiP54849. 4 interactions.
STRINGi9606.ENSP00000256951.

Polymorphism and mutation databases

BioMutaiEMP1.
DMDMi3041684.

Proteomic databases

PaxDbiP54849.
PRIDEiP54849.
TopDownProteomicsiP54849-1. [P54849-1]

Protocols and materials databases

DNASUi2012.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000256951; ENSP00000256951; ENSG00000134531. [P54849-1]
ENST00000396301; ENSP00000379595; ENSG00000134531. [P54849-2]
GeneIDi2012.
KEGGihsa:2012.
UCSCiuc001rbr.4. human. [P54849-1]

Organism-specific databases

CTDi2012.
GeneCardsiEMP1.
HGNCiHGNC:3333. EMP1.
HPAiHPA056250.
MIMi602333. gene.
neXtProtiNX_P54849.
PharmGKBiPA27770.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IW7W. Eukaryota.
ENOG410Z5WT. LUCA.
GeneTreeiENSGT00510000046328.
HOGENOMiHOG000059542.
HOVERGENiHBG001690.
InParanoidiP54849.
OMAiCWLCILV.
OrthoDBiEOG7F24VG.
PhylomeDBiP54849.
TreeFamiTF330414.

Miscellaneous databases

ChiTaRSiEMP1. human.
GeneWikiiEMP1.
GenomeRNAii2012.
PROiP54849.
SOURCEiSearch...

Gene expression databases

BgeeiP54849.
CleanExiHS_EMP1.
ExpressionAtlasiP54849. baseline and differential.
GenevisibleiP54849. HS.

Family and domain databases

InterProiIPR003932. EMP_1.
IPR004031. PMP22/EMP/MP20/Claudin.
IPR004032. PMP22_EMP_MP20.
[Graphical view]
PANTHERiPTHR10671:SF6. PTHR10671:SF6. 1 hit.
PfamiPF00822. PMP22_Claudin. 1 hit.
[Graphical view]
PRINTSiPR01453. EPMEMFAMILY.
PR01454. EPMEMPROT1.
PROSITEiPS01221. PMP22_1. 1 hit.
PS01222. PMP22_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a tumor-associated gene, a member of a novel family of genes encoding membrane glycoproteins."
    Ben-Porath I., Benvenisty N.
    Gene 183:69-75(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Fetal kidney.
  2. Ben-Porath I., Benvenisty N.
    Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 83.
  3. "cDNA cloning, genomic structure, and chromosome mapping of the human epithelial membrane protein CL-20 gene (EMP1), a member of the PMP22 family."
    Chen Y., Medvedev A., Ruzanov P., Marvin K.W., Jetten A.M.
    Genomics 41:40-48(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  4. "B4B, a novel growth-arrest gene, is expressed by a subset of progenitor/pre-B lymphocytes negative for cytoplasmic mu-chain."
    Ruegg C.L., Wu H., Fagnoni F.F., Engleman E.G., Laus R.
    J. Immunol. 157:72-80(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Blood.
  5. "Differential gene expression in human mammary carcinoma cells: identification of a new member of a receptor family."
    Schiemann S., Rueckels M., Engelholm L.H., Schwirzke M., Bruenner N., Weidle U.H.
    Anticancer Res. 17:13-20(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  6. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Tongue.
  7. "The finished DNA sequence of human chromosome 12."
    Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R.
    , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
    Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  8. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  9. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Liver.

Entry informationi

Entry nameiEMP1_HUMAN
AccessioniPrimary (citable) accession number: P54849
Secondary accession number(s): B2R5N1
, B4DRR1, O00681, Q13481, Q13834
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 15, 1998
Last modified: June 8, 2016
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.