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Protein

Transcription factor MafB

Gene

Mafb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a transcriptional activator or repressor. Plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells. Required for monocytic, macrophage, osteoclast, podocyte and islet beta cell differentiation. Involved in renal tubule survival and F4/80 maturation. Activates the insulin and glucagon promoters. Together with PAX6, transactivates weakly the glucagon gene promoter through the G1 element. SUMO modification controls its transcriptional activity and ability to specify macrophage fate. Binds element G1 on the glucagon promoter. Involved either as an oncogene or as a tumor suppressor, depending on the cell context.By similarity8 Publications

GO - Molecular functioni

  • DNA binding Source: MGI
  • protein heterodimerization activity Source: Ensembl
  • protein homodimerization activity Source: Ensembl
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB
  • transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB
  • transcription factor binding Source: MGI

GO - Biological processi

  • abducens nerve formation Source: UniProtKB
  • brain segmentation Source: MGI
  • inner ear morphogenesis Source: MGI
  • negative regulation of erythrocyte differentiation Source: MGI
  • negative regulation of osteoclast differentiation Source: UniProtKB
  • positive regulation of transcription by RNA polymerase II Source: MGI
  • regulation of transcription, DNA-templated Source: MGI
  • regulation of transcription by RNA polymerase II Source: MGI
  • respiratory gaseous exchange Source: MGI
  • rhombomere 5 development Source: MGI
  • rhombomere 6 development Source: MGI
  • segment specification Source: MGI
  • T cell differentiation in thymus Source: MGI
  • thymus development Source: MGI

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor MafB
Short name:
Maf-B
Alternative name(s):
Kreisler
Segmentation protein Kr
Transcription factor Maf-1
V-maf musculoaponeurotic fibrosarcoma oncogene homolog B
Gene namesi
Name:Mafb
Synonyms:Krml, Maf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:104555 Mafb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice show a defect in frequency of respiratory rhythm with a fatal apnea at birth due to lack of neurons from the preBoetC region. They displayed renal dysgenesis with abnormal podocyte differentiation as well as tubular apoptosis. They show altered actin-dependent macrophage morphology. They show a reduced number of cells expressing insulin and glucagon. Embryos also lack the abducens nerve which normaly innervates the lateral rectus muscle that is involved in eye movement (PubMed:27181683).6 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi32K → R: Loss of SUMO modification, increases transactivation activity, increases macrophage differentiation and inhibits myeloid progenitor growth; when associated with R-297. 1 Publication1
Mutagenesisi248N → A: Reduces ability to activate insulin and glucagon gene expression. 1 Publication1
Mutagenesisi248N → S: Loss of transcriptional activity. 1 Publication1
Mutagenesisi297K → R: Loss of SUMO modification, increases transactivation activity, increases macrophage differentiation and inhibits myeloid progenitor growth; when associated with R-32. 1 Publication1

Keywords - Diseasei

Proto-oncogene, Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000764951 – 323Transcription factor MafBAdd BLAST323

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki28Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki32Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternate1 Publication
Cross-linki32Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Cross-linki281Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki297Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)1 Publication

Post-translational modificationi

Sumoylated. Sumoylation on Lys-32 and Lys-297 stimulates its transcriptional repression activity and promotes macrophage differentiation from myeloid progenitors.1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP54841
PaxDbiP54841
PeptideAtlasiP54841
PRIDEiP54841

PTM databases

iPTMnetiP54841
PhosphoSitePlusiP54841

Expressioni

Tissue specificityi

Expressed in pancreatic alpha-cells (glucagon-positive cells), in podocytes of the kidney and macrophages (at protein level). Most abundant in kidney, gut, lung and brain.3 Publications

Developmental stagei

Expressed in pancreatic alpha-cells at 10.5 dpc. Expressed in insulin and glucagon islet progenitor cells at 12 dpc onwards (at protein level). Detectable at 8.0 dpc (one somite) as a band in the caudal hindbrain and by 8.5 dpc (six to eight somites) as a sharp rostral edge coincident with the rhombomeres (r) 4 and 5 boundary and a diffuse caudal edge located midway through r6. Expressed in the lens epithelial cells at 10.5 to 14.5 dpc.3 Publications

Gene expression databases

BgeeiENSMUSG00000074622
CleanExiMM_MAF1
GenevisibleiP54841 MM

Interactioni

Subunit structurei

Homodimer or heterodimer with other bHLH-Zip transcription factors. Forms homodimers and heterodimers with FOS, FOSB and FOSL2, but not with JUN proteins (JUN, JUNB and JUND). Interacts with the intracellular cytoplasmic domain of LRP1 (LRPICD); the interaction results in a moderate reduction of MAFB transcriptional potential (By similarity). Binds DNA as a homodimer or a heterodimer. Interacts with PAX6; the interaction is direct. Interacts with ETS1 and LRP1.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
FosP011014EBI-16093217,EBI-4288185

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201014, 5 interactors
DIPiDIP-60663N
IntActiP54841, 1 interactor
STRINGi10090.ENSMUSP00000096728

Structurei

Secondary structure

1323
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi215 – 218Combined sources4
Helixi221 – 225Combined sources5
Turni228 – 230Combined sources3
Helixi233 – 299Combined sources67

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WT7X-ray2.30B214-303[»]
2WTYX-ray2.90A/B211-306[»]
4AUWX-ray2.90A/B/E/F211-305[»]
ProteinModelPortaliP54841
SMRiP54841
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini238 – 301bZIPPROSITE-ProRule annotationAdd BLAST64

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni238 – 263Basic motifPROSITE-ProRule annotationAdd BLAST26
Regioni266 – 287Leucine-zipperPROSITE-ProRule annotationAdd BLAST22

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi131 – 143Poly-HisAdd BLAST13
Compositional biasi158 – 167Poly-His10

Domaini

The leucine-zipper domain is involved in the interaction with LRPICD.

Sequence similaritiesi

Belongs to the bZIP family. Maf subfamily.Curated

Phylogenomic databases

eggNOGiKOG4196 Eukaryota
ENOG41102C7 LUCA
GeneTreeiENSGT00550000074549
HOGENOMiHOG000261683
HOVERGENiHBG000313
InParanoidiP54841
KOiK09036
OMAiCNRLQPQ
OrthoDBiEOG091G0H46
PhylomeDBiP54841
TreeFamiTF325689

Family and domain databases

InterProiView protein in InterPro
IPR004827 bZIP
IPR004826 bZIP_Maf
IPR013592 Maf_TF_N
IPR028571 MafB
IPR008917 TF_DNA-bd_sf
IPR024874 Transciption_factor_Maf_fam
PANTHERiPTHR10129 PTHR10129, 1 hit
PTHR10129:SF10 PTHR10129:SF10, 1 hit
PfamiView protein in Pfam
PF03131 bZIP_Maf, 1 hit
PF08383 Maf_N, 1 hit
SMARTiView protein in SMART
SM00338 BRLZ, 1 hit
SUPFAMiSSF47454 SSF47454, 1 hit
PROSITEiView protein in PROSITE
PS50217 BZIP, 1 hit

Sequencei

Sequence statusi: Complete.

P54841-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAELSMGQE LPTSPLAMEY VNDFDLLKFD VKKEPLGRAE RPGRPCTRLQ
60 70 80 90 100
PAGSVSSTPL STPCSSVPSS PSFSPTEPKT HLEDLYWMAS NYQQMNPEAL
110 120 130 140 150
NLTPEDAVEA LIGSHPVPQP LQSFDGFRSA HHHHHHHHPH PHHGYPGAGV
160 170 180 190 200
THDDLGQHAH PHHHHHHQAS PPPSSAASPA QQLPTSHPGP GPHATAAATA
210 220 230 240 250
AGGNGSVEDR FSDDQLVSMS VRELNRHLRG FTKDEVIRLK QKRRTLKNRG
260 270 280 290 300
YAQSCRYKRV QQKHHLENEK TQLIQQVEQL KQEVSRLARE RDAYKVKCEK
310 320
LANSGFREAG STSDSPSSPE FFL
Length:323
Mass (Da):35,809
Last modified:October 1, 1996 - v1
Checksum:iD77AE07ABD9C2AD2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti275Q → K in BAE32860 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36435 mRNA Translation: AAA65689.1
AF180338 Genomic DNA Translation: AAD56221.1
AK132425 mRNA Translation: BAE21161.1
AK143209 mRNA Translation: BAE25306.1
AK154830 mRNA Translation: BAE32860.1
AL591665 Genomic DNA Translation: CAM21248.1
CH466551 Genomic DNA Translation: EDL06288.1
BC016434 mRNA Translation: AAH16434.1
BC038256 mRNA Translation: AAH38256.1
CCDSiCCDS16994.1
PIRiI49529
RefSeqiNP_034788.1, NM_010658.3
UniGeneiMm.330745

Genome annotation databases

EnsembliENSMUST00000099126; ENSMUSP00000096728; ENSMUSG00000074622
GeneIDi16658
KEGGimmu:16658
UCSCiuc008nqw.2 mouse

Similar proteinsi

Entry informationi

Entry nameiMAFB_MOUSE
AccessioniPrimary (citable) accession number: P54841
Secondary accession number(s): Q3U3C8, Q3UPT9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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