Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P54839 (HMCS_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxymethylglutaryl-CoA synthase

Short name=HMG-CoA synthase
EC=2.3.3.10
Alternative name(s):
3-hydroxy-3-methylglutaryl coenzyme A synthase
Gene names
Name:ERG13
Synonyms:HMGS
Ordered Locus Names:YML126C
ORF Names:YM4987.09C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length491 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase By similarity.

Catalytic activity

Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.

Pathway

Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.

Miscellaneous

Present with 34800 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the HMG-CoA synthase family.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 491491Hydroxymethylglutaryl-CoA synthase
PRO_0000213758

Sites

Active site1271Proton donor/acceptor By similarity
Active site1591Acyl-thioester intermediate By similarity
Active site2961Proton donor/acceptor By similarity

Amino acid modifications

Modified residue2761Phosphoserine Ref.5

Sequences

Sequence LengthMass (Da)Tools
P54839 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 44DFF3C0B0356D9E

FASTA49155,014
        10         20         30         40         50         60 
MKLSTKLCWC GIKGRLRPQK QQQLHNTNLQ MTELKKQKTA EQKTRPQNVG IKGIQIYIPT 

        70         80         90        100        110        120 
QCVNQSELEK FDGVSQGKYT IGLGQTNMSF VNDREDIYSM SLTVLSKLIK SYNIDTNKIG 

       130        140        150        160        170        180 
RLEVGTETLI DKSKSVKSVL MQLFGENTDV EGIDTLNACY GGTNALFNSL NWIESNAWDG 

       190        200        210        220        230        240 
RDAIVVCGDI AIYDKGAARP TGGAGTVAMW IGPDAPIVFD SVRASYMEHA YDFYKPDFTS 

       250        260        270        280        290        300 
EYPYVDGHFS LTCYVKALDQ VYKSYSKKAI SKGLVSDPAG SDALNVLKYF DYNVFHVPTC 

       310        320        330        340        350        360 
KLVTKSYGRL LYNDFRANPQ LFPEVDAELA TRDYDESLTD KNIEKTFVNV AKPFHKERVA 

       370        380        390        400        410        420 
QSLIVPTNTG NMYTASVYAA FASLLNYVGS DDLQGKRVGL FSYGSGLAAS LYSCKIVGDV 

       430        440        450        460        470        480 
QHIIKELDIT NKLAKRITET PKDYEAAIEL RENAHLKKNF KPQGSIEHLQ SGVYYLTNID 

       490 
DKFRRSYDVK K 

« Hide

References

« Hide 'large scale' references
[1]Kribii R., Cordier H., Karst F.
Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 28383 / FL100 / VTT C-80102.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-276, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X96617 Genomic DNA. Translation: CAA65437.1.
Z50178 Genomic DNA. Translation: CAA90557.1.
BK006946 Genomic DNA. Translation: DAA09773.1.
PIRS58202.
RefSeqNP_013580.1. NM_001182489.1.

3D structure databases

ProteinModelPortalP54839.
SMRP54839. Positions 48-487.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35079. 69 interactions.
DIPDIP-5071N.
IntActP54839. 36 interactions.
MINTMINT-546775.
STRING4932.YML126C.

Proteomic databases

MaxQBP54839.
PaxDbP54839.
PeptideAtlasP54839.
PRIDEP54839.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYML126C; YML126C; YML126C.
GeneID854913.
KEGGsce:YML126C.

Organism-specific databases

CYGDYML126c.
SGDS000004595. ERG13.

Phylogenomic databases

eggNOGCOG3425.
GeneTreeENSGT00390000006096.
HOGENOMHOG000012351.
KOK01641.
OMARDFTLND.
OrthoDBEOG7VHT69.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-648.
YEAST:YML126C-MONOMER.
BRENDA2.3.3.10. 984.
UniPathwayUPA00058; UER00102.

Gene expression databases

GenevestigatorP54839.

Family and domain databases

Gene3D3.40.47.10. 1 hit.
InterProIPR000590. HMG_CoA_synt_AS.
IPR013746. HMG_CoA_synt_C.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamPF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view]
SUPFAMSSF53901. SSF53901. 3 hits.
TIGRFAMsTIGR01833. HMG-CoA-S_euk. 1 hit.
PROSITEPS01226. HMG_COA_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio977914.

Entry information

Entry nameHMCS_YEAST
AccessionPrimary (citable) accession number: P54839
Secondary accession number(s): D6W0F9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways