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P54839

- HMCS_YEAST

UniProt

P54839 - HMCS_YEAST

Protein

Hydroxymethylglutaryl-CoA synthase

Gene

ERG13

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.By similarity

    Catalytic activityi

    Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei127 – 1271Proton donor/acceptorPROSITE-ProRule annotation
    Active sitei159 – 1591Acyl-thioester intermediatePROSITE-ProRule annotation
    Active sitei296 – 2961Proton donor/acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. hydroxymethylglutaryl-CoA synthase activity Source: SGD

    GO - Biological processi

    1. ergosterol biosynthetic process Source: SGD
    2. isoprenoid biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-648.
    YEAST:YML126C-MONOMER.
    BRENDAi2.3.3.10. 984.
    ReactomeiREACT_188982. Activation of gene expression by SREBF (SREBP).
    REACT_188984. PPARA activates gene expression.
    UniPathwayiUPA00058; UER00102.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hydroxymethylglutaryl-CoA synthase (EC:2.3.3.10)
    Short name:
    HMG-CoA synthase
    Alternative name(s):
    3-hydroxy-3-methylglutaryl coenzyme A synthase
    Gene namesi
    Name:ERG13
    Synonyms:HMGS
    Ordered Locus Names:YML126C
    ORF Names:YM4987.09C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYML126c.
    SGDiS000004595. ERG13.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 491491Hydroxymethylglutaryl-CoA synthasePRO_0000213758Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei276 – 2761Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP54839.
    PaxDbiP54839.
    PeptideAtlasiP54839.
    PRIDEiP54839.

    Expressioni

    Gene expression databases

    GenevestigatoriP54839.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ARO8P530901EBI-8373,EBI-2042933
    RNR1P215241EBI-8373,EBI-15234
    RPB5P204341EBI-8373,EBI-15781

    Protein-protein interaction databases

    BioGridi35079. 69 interactions.
    DIPiDIP-5071N.
    IntActiP54839. 36 interactions.
    MINTiMINT-546775.
    STRINGi4932.YML126C.

    Structurei

    3D structure databases

    ProteinModelPortaliP54839.
    SMRiP54839. Positions 48-487.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the HMG-CoA synthase family.Curated

    Phylogenomic databases

    eggNOGiCOG3425.
    GeneTreeiENSGT00390000006096.
    HOGENOMiHOG000012351.
    KOiK01641.
    OMAiRDFTLND.
    OrthoDBiEOG7VHT69.

    Family and domain databases

    Gene3Di3.40.47.10. 1 hit.
    InterProiIPR000590. HMG_CoA_synt_AS.
    IPR013746. HMG_CoA_synt_C_dom.
    IPR013528. HMG_CoA_synth_N.
    IPR010122. HMG_CoA_synthase_euk.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view]
    PfamiPF08540. HMG_CoA_synt_C. 1 hit.
    PF01154. HMG_CoA_synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF53901. SSF53901. 3 hits.
    TIGRFAMsiTIGR01833. HMG-CoA-S_euk. 1 hit.
    PROSITEiPS01226. HMG_COA_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P54839-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLSTKLCWC GIKGRLRPQK QQQLHNTNLQ MTELKKQKTA EQKTRPQNVG    50
    IKGIQIYIPT QCVNQSELEK FDGVSQGKYT IGLGQTNMSF VNDREDIYSM 100
    SLTVLSKLIK SYNIDTNKIG RLEVGTETLI DKSKSVKSVL MQLFGENTDV 150
    EGIDTLNACY GGTNALFNSL NWIESNAWDG RDAIVVCGDI AIYDKGAARP 200
    TGGAGTVAMW IGPDAPIVFD SVRASYMEHA YDFYKPDFTS EYPYVDGHFS 250
    LTCYVKALDQ VYKSYSKKAI SKGLVSDPAG SDALNVLKYF DYNVFHVPTC 300
    KLVTKSYGRL LYNDFRANPQ LFPEVDAELA TRDYDESLTD KNIEKTFVNV 350
    AKPFHKERVA QSLIVPTNTG NMYTASVYAA FASLLNYVGS DDLQGKRVGL 400
    FSYGSGLAAS LYSCKIVGDV QHIIKELDIT NKLAKRITET PKDYEAAIEL 450
    RENAHLKKNF KPQGSIEHLQ SGVYYLTNID DKFRRSYDVK K 491
    Length:491
    Mass (Da):55,014
    Last modified:October 1, 1996 - v1
    Checksum:i44DFF3C0B0356D9E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X96617 Genomic DNA. Translation: CAA65437.1.
    Z50178 Genomic DNA. Translation: CAA90557.1.
    BK006946 Genomic DNA. Translation: DAA09773.1.
    PIRiS58202.
    RefSeqiNP_013580.1. NM_001182489.1.

    Genome annotation databases

    EnsemblFungiiYML126C; YML126C; YML126C.
    GeneIDi854913.
    KEGGisce:YML126C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X96617 Genomic DNA. Translation: CAA65437.1 .
    Z50178 Genomic DNA. Translation: CAA90557.1 .
    BK006946 Genomic DNA. Translation: DAA09773.1 .
    PIRi S58202.
    RefSeqi NP_013580.1. NM_001182489.1.

    3D structure databases

    ProteinModelPortali P54839.
    SMRi P54839. Positions 48-487.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35079. 69 interactions.
    DIPi DIP-5071N.
    IntActi P54839. 36 interactions.
    MINTi MINT-546775.
    STRINGi 4932.YML126C.

    Proteomic databases

    MaxQBi P54839.
    PaxDbi P54839.
    PeptideAtlasi P54839.
    PRIDEi P54839.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YML126C ; YML126C ; YML126C .
    GeneIDi 854913.
    KEGGi sce:YML126C.

    Organism-specific databases

    CYGDi YML126c.
    SGDi S000004595. ERG13.

    Phylogenomic databases

    eggNOGi COG3425.
    GeneTreei ENSGT00390000006096.
    HOGENOMi HOG000012351.
    KOi K01641.
    OMAi RDFTLND.
    OrthoDBi EOG7VHT69.

    Enzyme and pathway databases

    UniPathwayi UPA00058 ; UER00102 .
    BioCyci MetaCyc:MONOMER-648.
    YEAST:YML126C-MONOMER.
    BRENDAi 2.3.3.10. 984.
    Reactomei REACT_188982. Activation of gene expression by SREBF (SREBP).
    REACT_188984. PPARA activates gene expression.

    Miscellaneous databases

    NextBioi 977914.

    Gene expression databases

    Genevestigatori P54839.

    Family and domain databases

    Gene3Di 3.40.47.10. 1 hit.
    InterProi IPR000590. HMG_CoA_synt_AS.
    IPR013746. HMG_CoA_synt_C_dom.
    IPR013528. HMG_CoA_synth_N.
    IPR010122. HMG_CoA_synthase_euk.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view ]
    Pfami PF08540. HMG_CoA_synt_C. 1 hit.
    PF01154. HMG_CoA_synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53901. SSF53901. 3 hits.
    TIGRFAMsi TIGR01833. HMG-CoA-S_euk. 1 hit.
    PROSITEi PS01226. HMG_COA_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Kribii R., Cordier H., Karst F.
      Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 28383 / FL100 / VTT C-80102.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-276, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiHMCS_YEAST
    AccessioniPrimary (citable) accession number: P54839
    Secondary accession number(s): D6W0F9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 127 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 34800 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3