P54839 (HMCS_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxymethylglutaryl-CoA synthase
Short name=HMG-CoA synthase
EC=2.3.3.10

Alternative name(s):
3-hydroxy-3-methylglutaryl coenzyme A synthase

Gene names

Name:	ERG13
Synonyms:	HMGS
Ordered Locus Names:	YML126C
ORF Names:	YM4987.09C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase By similarity.
Catalytic activity	Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.
Miscellaneous	Present with 34800 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the HMG-CoA synthase family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Molecular function	Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ergosterol biosynthetic process Inferred from mutant phenotype. Source: SGD isoprenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	hydroxymethylglutaryl-CoA synthase activity Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ARO8	P53090	1	EBI-8373,EBI-2042933
RNR1	P21524	1	EBI-8373,EBI-15234
RPB5	P20434	1	EBI-8373,EBI-15781

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 491	491	Hydroxymethylglutaryl-CoA synthase		PRO_0000213758
Sites
	Active site	159	1	Potential
Amino acid modifications
	Modified residue	276	1	Phosphoserine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P54839 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 44DFF3C0B0356D9E	FASTA	491	55,014
	10 20 30 40 50 60 MKLSTKLCWC GIKGRLRPQK QQQLHNTNLQ MTELKKQKTA EQKTRPQNVG IKGIQIYIPT 70 80 90 100 110 120 QCVNQSELEK FDGVSQGKYT IGLGQTNMSF VNDREDIYSM SLTVLSKLIK SYNIDTNKIG 130 140 150 160 170 180 RLEVGTETLI DKSKSVKSVL MQLFGENTDV EGIDTLNACY GGTNALFNSL NWIESNAWDG 190 200 210 220 230 240 RDAIVVCGDI AIYDKGAARP TGGAGTVAMW IGPDAPIVFD SVRASYMEHA YDFYKPDFTS 250 260 270 280 290 300 EYPYVDGHFS LTCYVKALDQ VYKSYSKKAI SKGLVSDPAG SDALNVLKYF DYNVFHVPTC 310 320 330 340 350 360 KLVTKSYGRL LYNDFRANPQ LFPEVDAELA TRDYDESLTD KNIEKTFVNV AKPFHKERVA 370 380 390 400 410 420 QSLIVPTNTG NMYTASVYAA FASLLNYVGS DDLQGKRVGL FSYGSGLAAS LYSCKIVGDV 430 440 450 460 470 480 QHIIKELDIT NKLAKRITET PKDYEAAIEL RENAHLKKNF KPQGSIEHLQ SGVYYLTNID 490 DKFRRSYDVK K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Kribii R., Cordier H., Karst F. Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 28383 / FL100 / VTT C-80102.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-276, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X96617 Genomic DNA. Translation: CAA65437.1. Z50178 Genomic DNA. Translation: CAA90557.1. BK006946 Genomic DNA. Translation: DAA09773.1.
PIR	S58202.
RefSeq	NP_013580.1. NM_001182489.1.
3D structure databases
ProteinModelPortal	P54839.
SMR	P54839. Positions 47-490.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5071N.
IntAct	P54839. 40 interactions.
MINT	MINT-546775.
STRING	P54839.
Proteomic databases
PeptideAtlas	P54839.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML126C; YML126C; YML126C.
GeneID	854913.
KEGG	sce:YML126C.
NMPDR	fig|4932.3.peg.4616.
Organism-specific databases
CYGD	YML126c.
SGD	S000004595. ERG13.
Phylogenomic databases
eggNOG	fuNOG04295.
GeneTree	EFGT00050000005944.
HOGENOM	HBG578402.
OMA	WIESSYW.
OrthoDB	EOG4G7G73.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-648.
BRENDA	2.3.3.10. 984.
Gene expression databases
ArrayExpress	P54839.
Genevestigator	P54839.
GermOnline	YML126C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000590. HMG_CoA_synt_AS. IPR013746. HMG_CoA_synt_C. IPR013528. HMG_CoA_synth_N. IPR010122. HMG_CoA_synthase_euk. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 1 hit.
KO	K01641.
Pfam	PF08540. HMG_CoA_synt_C. 1 hit. PF01154. HMG_CoA_synt_N. 1 hit. [Graphical view]
SUPFAM	SSF53901. Thiolase-like. 2 hits.
TIGRFAMs	TIGR01833. HMG-CoA-S_euk. 1 hit.
PROSITE	PS01226. HMG_COA_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977914.

Entry information

Entry name

HMCS_YEAST

Accession

Primary (citable) accession number: P54839
Secondary accession number(s): D6W0F9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program