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P54839

- HMCS_YEAST

UniProt

P54839 - HMCS_YEAST

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Protein

Hydroxymethylglutaryl-CoA synthase

Gene

ERG13

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.By similarity

Catalytic activityi

Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei127 – 1271Proton donor/acceptorPROSITE-ProRule annotation
Active sitei159 – 1591Acyl-thioester intermediatePROSITE-ProRule annotation
Active sitei296 – 2961Proton donor/acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. hydroxymethylglutaryl-CoA synthase activity Source: SGD

GO - Biological processi

  1. ergosterol biosynthetic process Source: SGD
  2. isoprenoid biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-648.
YEAST:YML126C-MONOMER.
BRENDAi2.3.3.10. 984.
ReactomeiREACT_188982. Activation of gene expression by SREBF (SREBP).
REACT_188984. PPARA activates gene expression.
REACT_240742. Synthesis of Ketone Bodies.
REACT_253879. Cholesterol biosynthesis.
UniPathwayiUPA00058; UER00102.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxymethylglutaryl-CoA synthase (EC:2.3.3.10)
Short name:
HMG-CoA synthase
Alternative name(s):
3-hydroxy-3-methylglutaryl coenzyme A synthase
Gene namesi
Name:ERG13
Synonyms:HMGS
Ordered Locus Names:YML126C
ORF Names:YM4987.09C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYML126c.
SGDiS000004595. ERG13.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 491491Hydroxymethylglutaryl-CoA synthasePRO_0000213758Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei276 – 2761Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP54839.
PaxDbiP54839.
PeptideAtlasiP54839.
PRIDEiP54839.

Expressioni

Gene expression databases

GenevestigatoriP54839.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ARO8P530901EBI-8373,EBI-2042933
RNR1P215241EBI-8373,EBI-15234
RPB5P204341EBI-8373,EBI-15781

Protein-protein interaction databases

BioGridi35079. 70 interactions.
DIPiDIP-5071N.
IntActiP54839. 36 interactions.
MINTiMINT-546775.
STRINGi4932.YML126C.

Structurei

3D structure databases

ProteinModelPortaliP54839.
SMRiP54839. Positions 48-487.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HMG-CoA synthase family.Curated

Phylogenomic databases

eggNOGiCOG3425.
GeneTreeiENSGT00390000006096.
HOGENOMiHOG000012351.
InParanoidiP54839.
KOiK01641.
OMAiRDFTLND.
OrthoDBiEOG7VHT69.

Family and domain databases

Gene3Di3.40.47.10. 1 hit.
InterProiIPR000590. HMG_CoA_synt_AS.
IPR013746. HMG_CoA_synt_C_dom.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF53901. SSF53901. 3 hits.
TIGRFAMsiTIGR01833. HMG-CoA-S_euk. 1 hit.
PROSITEiPS01226. HMG_COA_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54839-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLSTKLCWC GIKGRLRPQK QQQLHNTNLQ MTELKKQKTA EQKTRPQNVG
60 70 80 90 100
IKGIQIYIPT QCVNQSELEK FDGVSQGKYT IGLGQTNMSF VNDREDIYSM
110 120 130 140 150
SLTVLSKLIK SYNIDTNKIG RLEVGTETLI DKSKSVKSVL MQLFGENTDV
160 170 180 190 200
EGIDTLNACY GGTNALFNSL NWIESNAWDG RDAIVVCGDI AIYDKGAARP
210 220 230 240 250
TGGAGTVAMW IGPDAPIVFD SVRASYMEHA YDFYKPDFTS EYPYVDGHFS
260 270 280 290 300
LTCYVKALDQ VYKSYSKKAI SKGLVSDPAG SDALNVLKYF DYNVFHVPTC
310 320 330 340 350
KLVTKSYGRL LYNDFRANPQ LFPEVDAELA TRDYDESLTD KNIEKTFVNV
360 370 380 390 400
AKPFHKERVA QSLIVPTNTG NMYTASVYAA FASLLNYVGS DDLQGKRVGL
410 420 430 440 450
FSYGSGLAAS LYSCKIVGDV QHIIKELDIT NKLAKRITET PKDYEAAIEL
460 470 480 490
RENAHLKKNF KPQGSIEHLQ SGVYYLTNID DKFRRSYDVK K
Length:491
Mass (Da):55,014
Last modified:October 1, 1996 - v1
Checksum:i44DFF3C0B0356D9E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96617 Genomic DNA. Translation: CAA65437.1.
Z50178 Genomic DNA. Translation: CAA90557.1.
BK006946 Genomic DNA. Translation: DAA09773.1.
PIRiS58202.
RefSeqiNP_013580.1. NM_001182489.1.

Genome annotation databases

EnsemblFungiiYML126C; YML126C; YML126C.
GeneIDi854913.
KEGGisce:YML126C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96617 Genomic DNA. Translation: CAA65437.1 .
Z50178 Genomic DNA. Translation: CAA90557.1 .
BK006946 Genomic DNA. Translation: DAA09773.1 .
PIRi S58202.
RefSeqi NP_013580.1. NM_001182489.1.

3D structure databases

ProteinModelPortali P54839.
SMRi P54839. Positions 48-487.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35079. 70 interactions.
DIPi DIP-5071N.
IntActi P54839. 36 interactions.
MINTi MINT-546775.
STRINGi 4932.YML126C.

Proteomic databases

MaxQBi P54839.
PaxDbi P54839.
PeptideAtlasi P54839.
PRIDEi P54839.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YML126C ; YML126C ; YML126C .
GeneIDi 854913.
KEGGi sce:YML126C.

Organism-specific databases

CYGDi YML126c.
SGDi S000004595. ERG13.

Phylogenomic databases

eggNOGi COG3425.
GeneTreei ENSGT00390000006096.
HOGENOMi HOG000012351.
InParanoidi P54839.
KOi K01641.
OMAi RDFTLND.
OrthoDBi EOG7VHT69.

Enzyme and pathway databases

UniPathwayi UPA00058 ; UER00102 .
BioCyci MetaCyc:MONOMER-648.
YEAST:YML126C-MONOMER.
BRENDAi 2.3.3.10. 984.
Reactomei REACT_188982. Activation of gene expression by SREBF (SREBP).
REACT_188984. PPARA activates gene expression.
REACT_240742. Synthesis of Ketone Bodies.
REACT_253879. Cholesterol biosynthesis.

Miscellaneous databases

NextBioi 977914.

Gene expression databases

Genevestigatori P54839.

Family and domain databases

Gene3Di 3.40.47.10. 1 hit.
InterProi IPR000590. HMG_CoA_synt_AS.
IPR013746. HMG_CoA_synt_C_dom.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view ]
Pfami PF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view ]
SUPFAMi SSF53901. SSF53901. 3 hits.
TIGRFAMsi TIGR01833. HMG-CoA-S_euk. 1 hit.
PROSITEi PS01226. HMG_COA_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Kribii R., Cordier H., Karst F.
    Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 28383 / FL100 / VTT C-80102.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-276, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHMCS_YEAST
AccessioniPrimary (citable) accession number: P54839
Secondary accession number(s): D6W0F9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 26, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 34800 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3