Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Hydroxymethylglutaryl-CoA synthase

Gene

ERG13

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.By similarity

Miscellaneous

Present with 34800 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.PROSITE-ProRule annotation

Pathwayi: (R)-mevalonate biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes (R)-mevalonate from acetyl-CoA.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Acetyl-CoA acetyltransferase (ERG10)
  2. Hydroxymethylglutaryl-CoA synthase (ERG13)
  3. 3-hydroxy-3-methylglutaryl-coenzyme A reductase 2 (HMG2), 3-hydroxy-3-methylglutaryl-coenzyme A reductase 1 (HMG1)
This subpathway is part of the pathway (R)-mevalonate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-mevalonate from acetyl-CoA, the pathway (R)-mevalonate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei127Proton donor/acceptorPROSITE-ProRule annotation1
Active sitei159Acyl-thioester intermediatePROSITE-ProRule annotation1
Active sitei296Proton donor/acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

  • hydroxymethylglutaryl-CoA synthase activity Source: SGD

GO - Biological processi

  • ergosterol biosynthetic process Source: SGD
  • isoprenoid biosynthetic process Source: InterPro

Keywordsi

Molecular functionTransferase
Biological processLipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Enzyme and pathway databases

BioCyciMetaCyc:YML126C-MONOMER
YEAST:YML126C-MONOMER
BRENDAi2.3.3.10 984
ReactomeiR-SCE-191273 Cholesterol biosynthesis
R-SCE-77111 Synthesis of Ketone Bodies
UniPathwayiUPA00058; UER00102

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxymethylglutaryl-CoA synthase (EC:2.3.3.10)
Short name:
HMG-CoA synthase
Alternative name(s):
3-hydroxy-3-methylglutaryl coenzyme A synthase
Gene namesi
Name:ERG13
Synonyms:HMGS
Ordered Locus Names:YML126C
ORF Names:YM4987.09C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML126C
SGDiS000004595 ERG13

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002137581 – 491Hydroxymethylglutaryl-CoA synthaseAdd BLAST491

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei276PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP54839
PaxDbiP54839
PRIDEiP54839

PTM databases

iPTMnetiP54839

Interactioni

Protein-protein interaction databases

BioGridi35079, 265 interactors
DIPiDIP-5071N
IntActiP54839, 36 interactors
MINTiP54839
STRINGi4932.YML126C

Structurei

3D structure databases

ProteinModelPortaliP54839
SMRiP54839
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HMG-CoA synthase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000006096
HOGENOMiHOG000012351
InParanoidiP54839
KOiK01641
OMAiATHMEHA
OrthoDBiEOG092C22BG

Family and domain databases

Gene3Di3.40.47.10, 1 hit
InterProiView protein in InterPro
IPR000590 HMG_CoA_synt_AS
IPR013746 HMG_CoA_synt_C_dom
IPR013528 HMG_CoA_synth_N
IPR010122 HMG_CoA_synthase_euk
IPR016039 Thiolase-like
PfamiView protein in Pfam
PF08540 HMG_CoA_synt_C, 1 hit
PF01154 HMG_CoA_synt_N, 1 hit
SUPFAMiSSF53901 SSF53901, 3 hits
TIGRFAMsiTIGR01833 HMG-CoA-S_euk, 1 hit
PROSITEiView protein in PROSITE
PS01226 HMG_COA_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

P54839-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLSTKLCWC GIKGRLRPQK QQQLHNTNLQ MTELKKQKTA EQKTRPQNVG
60 70 80 90 100
IKGIQIYIPT QCVNQSELEK FDGVSQGKYT IGLGQTNMSF VNDREDIYSM
110 120 130 140 150
SLTVLSKLIK SYNIDTNKIG RLEVGTETLI DKSKSVKSVL MQLFGENTDV
160 170 180 190 200
EGIDTLNACY GGTNALFNSL NWIESNAWDG RDAIVVCGDI AIYDKGAARP
210 220 230 240 250
TGGAGTVAMW IGPDAPIVFD SVRASYMEHA YDFYKPDFTS EYPYVDGHFS
260 270 280 290 300
LTCYVKALDQ VYKSYSKKAI SKGLVSDPAG SDALNVLKYF DYNVFHVPTC
310 320 330 340 350
KLVTKSYGRL LYNDFRANPQ LFPEVDAELA TRDYDESLTD KNIEKTFVNV
360 370 380 390 400
AKPFHKERVA QSLIVPTNTG NMYTASVYAA FASLLNYVGS DDLQGKRVGL
410 420 430 440 450
FSYGSGLAAS LYSCKIVGDV QHIIKELDIT NKLAKRITET PKDYEAAIEL
460 470 480 490
RENAHLKKNF KPQGSIEHLQ SGVYYLTNID DKFRRSYDVK K
Length:491
Mass (Da):55,014
Last modified:October 1, 1996 - v1
Checksum:i44DFF3C0B0356D9E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96617 Genomic DNA Translation: CAA65437.1
Z50178 Genomic DNA Translation: CAA90557.1
BK006946 Genomic DNA Translation: DAA09773.1
PIRiS58202
RefSeqiNP_013580.1, NM_001182489.1

Genome annotation databases

EnsemblFungiiYML126C; YML126C; YML126C
GeneIDi854913
KEGGisce:YML126C

Similar proteinsi

Entry informationi

Entry nameiHMCS_YEAST
AccessioniPrimary (citable) accession number: P54839
Secondary accession number(s): D6W0F9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health