P54838 (DAK1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxyacetone kinase 1
Short name=DHA kinase 1
EC=2.7.1.29

Alternative name(s):
Glycerone kinase 1

Gene names

Name:	DAK1
Ordered Locus Names:	YML070W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	584 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + glycerone = ADP + glycerone phosphate.
Pathway	Polyol metabolism; glycerol fermentation; glycerone phosphate from glycerol (oxidative route): step 2/2.
Miscellaneous	Present with 23600 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the dihydroxyacetone kinase (DAK) family. Contains 1 DhaK domain. Contains 1 DhaL domain.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	glycerol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerone kinase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 584	584	Dihydroxyacetone kinase 1		PRO_0000121522
Regions
	Domain	7 – 353	347	DhaK
	Domain	386 – 582	197	DhaL
	Nucleotide binding	415 – 418	4	ATP By similarity
	Nucleotide binding	459 – 460	2	ATP By similarity
	Nucleotide binding	514 – 515	2	ATP By similarity
	Nucleotide binding	567 – 569	3	ATP By similarity
	Region	51 – 54	4	Substrate binding By similarity
Sites
	Active site	220	1	Tele-hemiaminal-histidine intermediate By similarity
	Binding site	103	1	Substrate By similarity
	Binding site	108	1	Substrate By similarity
Amino acid modifications
	Modified residue	5	1	Phosphoserine Ref.6
	Modified residue	25	1	Phosphoserine Ref.6
	Modified residue	233	1	Phosphoserine Ref.6
	Modified residue	365	1	Phosphoserine Ref.5 Ref.6
	Modified residue	563	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P54838 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 3B488A5649FAFE25	FASTA	584	62,207
	10 20 30 40 50 60 MSAKSFEVTD PVNSSLKGFA LANPSITLVP EEKILFRKTD SDKIALISGG GSGHEPTHAG 70 80 90 100 110 120 FIGKGMLSGA VVGEIFASPS TKQILNAIRL VNENASGVLL IVKNYTGDVL HFGLSAERAR 130 140 150 160 170 180 ALGINCRVAV IGDDVAVGRE KGGMVGRRAL AGTVLVHKIV GAFAEEYSSK YGLDGTAKVA 190 200 210 220 230 240 KIINDNLVTI GSSLDHCKVP GRKFESELNE KQMELGMGIH NEPGVKVLDP IPSTEDLISK 250 260 270 280 290 300 YMLPKLLDPN DKDRAFVKFD EDDEVVLLVN NLGGVSNFVI SSITSKTTDF LKENYNITPV 310 320 330 340 350 360 QTIAGTLMTS FNGNGFSITL LNATKATKAL QSDFEEIKSV LDLLNAFTNA PGWPIADFEK 370 380 390 400 410 420 TSAPSVNDDL LHNEVTAKAV GTYDFDKFAE WMKSGAEQVI KSEPHITELD NQVGDGDCGY 430 440 450 460 470 480 TLVAGVKGIT ENLDKLSKDS LSQAVAQISD FIEGSMGGTS GGLYSILLSG FSHGLIQVCK 490 500 510 520 530 540 SKDEPVTKEI VAKSLGIALD TLYKYTKARK GSSTMIDALE PFVKEFTASK DFNKAVKAAE 550 560 570 580 EGAKSTATFE AKFGRASYVG DSSQVEDPGA VGLCEFLKGV QSAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Metabolic and regulatory changes associated with growth of Saccharomyces cerevisiae in 1.4 M NaCl. Evidence for osmotic induction of glycerol dissimilation via the dihydroxyacetone pathway." Norbeck J., Blomberg A. J. Biol. Chem. 272:5544-5554(1997) [PubMed: 9038161] [Abstract] Cited for: PROTEIN SEQUENCE OF 104-112 AND 159-167, PARTIAL CHARACTERIZATION. Strain: ATCC 44827 / SKQ2N.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-365, MASS SPECTROMETRY.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-5; SER-25; SER-233; SER-365 AND SER-563, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z38114 Genomic DNA. Translation: CAA86250.1. BK006946 Genomic DNA. Translation: DAA09827.1.
PIR	S48327.
RefSeq	NP_013641.1. NM_001182429.1.
3D structure databases
ProteinModelPortal	P54838.
SMR	P54838. Positions 3-355, 381-584.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4531N.
IntAct	P54838. 2 interactions.
MINT	MINT-478762.
STRING	P54838.
Proteomic databases
PeptideAtlas	P54838.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML070W; YML070W; YML070W.
GeneID	854932.
KEGG	sce:YML070W.
NMPDR	fig|4932.3.peg.4678.
Organism-specific databases
CYGD	YML070w.
SGD	S000004535. DAK1.
Phylogenomic databases
eggNOG	fuNOG05468.
GeneTree	EFGT00050000006766.
HOGENOM	HBG473357.
OMA	HCKVPGR.
OrthoDB	EOG46T68Z.
Enzyme and pathway databases
BRENDA	2.7.1.29. 984.
Gene expression databases
ArrayExpress	P54838.
Genevestigator	P54838.
GermOnline	YML070W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004006. Dak1. IPR004007. Dak2. IPR012734. DhaK_ATP. [Graphical view]
KO	K00863.
Pfam	PF02733. Dak1. 1 hit. PF02734. Dak2. 1 hit. [Graphical view]
SUPFAM	SSF101473. Dak2. 1 hit.
TIGRFAMs	TIGR02361. Dak_ATP. 1 hit.
PROSITE	PS51481. DHAK. 1 hit. PS51480. DHAL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977965.

Entry information

Entry name

DAK1_YEAST

Accession

Primary (citable) accession number: P54838
Secondary accession number(s): D6VZA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program