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Protein

Dihydroxyacetone kinase 1

Gene

DAK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde.By similarity

Miscellaneous

Present with 23600 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + glycerone = ADP + glycerone phosphate.
ATP + D-glyceraldehyde = ADP + D-glyceraldehyde 3-phosphate.

Pathwayi: glycerol fermentation

This protein is involved in step 2 of the subpathway that synthesizes glycerone phosphate from glycerol (oxidative route).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Dihydroxyacetone kinase 1 (DAK1), Dihydroxyacetone kinase 2 (DAK2)
This subpathway is part of the pathway glycerol fermentation, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycerone phosphate from glycerol (oxidative route), the pathway glycerol fermentation and in Polyol metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei103SubstrateBy similarity1
Binding sitei108SubstrateBy similarity1
Active sitei220Tele-hemiaminal-histidine intermediatePROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi415 – 418ATPBy similarity4
Nucleotide bindingi459 – 460ATPBy similarity2
Nucleotide bindingi514 – 515ATPBy similarity2
Nucleotide bindingi567 – 569ATPBy similarity3

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • glycerone kinase activity Source: SGD
  • triokinase activity Source: UniProtKB-EC

GO - Biological processi

  • anaerobic glycerol catabolic process Source: UniProtKB-UniPathway
  • cellular response to toxic substance Source: SGD
  • glycerol catabolic process Source: SGD

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycerol metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:YML070W-MONOMER
YEAST:YML070W-MONOMER
BRENDAi2.7.1.29 984
ReactomeiR-SCE-70350 Fructose catabolism
UniPathwayiUPA00617; UER00669

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydroxyacetone kinase 1 (EC:2.7.1.28, EC:2.7.1.29)
Short name:
DHA kinase 1
Alternative name(s):
Glycerone kinase 1
Triokinase 1
Triose kinase 1
Gene namesi
Name:DAK1
Ordered Locus Names:YML070W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML070W
SGDiS000004535 DAK1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001215222 – 584Dihydroxyacetone kinase 1Add BLAST583

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei2PhosphoserineCombined sources1
Modified residuei5PhosphoserineCombined sources1
Modified residuei365PhosphoserineCombined sources1
Modified residuei512PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP54838
PaxDbiP54838
PRIDEiP54838

PTM databases

iPTMnetiP54838

Interactioni

Protein-protein interaction databases

BioGridi35096, 56 interactors
DIPiDIP-4531N
IntActiP54838, 7 interactors
STRINGi4932.YML070W

Structurei

3D structure databases

ProteinModelPortaliP54838
SMRiP54838
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 353DhaKPROSITE-ProRule annotationAdd BLAST347
Domaini386 – 582DhaLPROSITE-ProRule annotationAdd BLAST197

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni51 – 54Substrate bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000015415
HOGENOMiHOG000234158
InParanoidiP54838
KOiK00863
OMAiAWPNVAK
OrthoDBiEOG092C1OGC

Family and domain databases

Gene3Di1.25.40.340, 1 hit
InterProiView protein in InterPro
IPR012734 DhaK_ATP
IPR004006 DhaK_dom
IPR004007 DhaL_dom
IPR036117 DhaL_dom_sf
PfamiView protein in Pfam
PF02733 Dak1, 1 hit
PF02734 Dak2, 1 hit
SMARTiView protein in SMART
SM01120 Dak2, 1 hit
SUPFAMiSSF101473 SSF101473, 1 hit
TIGRFAMsiTIGR02361 dak_ATP, 1 hit
PROSITEiView protein in PROSITE
PS51481 DHAK, 1 hit
PS51480 DHAL, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P54838-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAKSFEVTD PVNSSLKGFA LANPSITLVP EEKILFRKTD SDKIALISGG
60 70 80 90 100
GSGHEPTHAG FIGKGMLSGA VVGEIFASPS TKQILNAIRL VNENASGVLL
110 120 130 140 150
IVKNYTGDVL HFGLSAERAR ALGINCRVAV IGDDVAVGRE KGGMVGRRAL
160 170 180 190 200
AGTVLVHKIV GAFAEEYSSK YGLDGTAKVA KIINDNLVTI GSSLDHCKVP
210 220 230 240 250
GRKFESELNE KQMELGMGIH NEPGVKVLDP IPSTEDLISK YMLPKLLDPN
260 270 280 290 300
DKDRAFVKFD EDDEVVLLVN NLGGVSNFVI SSITSKTTDF LKENYNITPV
310 320 330 340 350
QTIAGTLMTS FNGNGFSITL LNATKATKAL QSDFEEIKSV LDLLNAFTNA
360 370 380 390 400
PGWPIADFEK TSAPSVNDDL LHNEVTAKAV GTYDFDKFAE WMKSGAEQVI
410 420 430 440 450
KSEPHITELD NQVGDGDCGY TLVAGVKGIT ENLDKLSKDS LSQAVAQISD
460 470 480 490 500
FIEGSMGGTS GGLYSILLSG FSHGLIQVCK SKDEPVTKEI VAKSLGIALD
510 520 530 540 550
TLYKYTKARK GSSTMIDALE PFVKEFTASK DFNKAVKAAE EGAKSTATFE
560 570 580
AKFGRASYVG DSSQVEDPGA VGLCEFLKGV QSAL
Length:584
Mass (Da):62,207
Last modified:October 1, 1996 - v1
Checksum:i3B488A5649FAFE25
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38114 Genomic DNA Translation: CAA86250.1
BK006946 Genomic DNA Translation: DAA09827.1
PIRiS48327
RefSeqiNP_013641.1, NM_001182429.1

Genome annotation databases

EnsemblFungiiYML070W; YML070W; YML070W
GeneIDi854932
KEGGisce:YML070W

Similar proteinsi

Entry informationi

Entry nameiDAK1_YEAST
AccessioniPrimary (citable) accession number: P54838
Secondary accession number(s): D6VZA3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health