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P54837

- TMEDA_YEAST

UniProt

P54837 - TMEDA_YEAST

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Protein
Endoplasmic reticulum vesicle protein 25
Gene
ERV25, YML012W, YM9571.06
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Constituent of COPII-coated endoplasmic reticulum-derived transport vesicles. Required for efficient transport of a subset of secretory proteins to the Golgi. Possesses a C-terminal dilysine motif that interacts with COPI coat subunits. Facilitates retrograde transport from the Golgi to the endoplasmic reticulum.2 Publications

GO - Molecular functioni

  1. protein binding Source: IntAct

GO - Biological processi

  1. ER to Golgi vesicle-mediated transport Source: SGD
  2. protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32616-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoplasmic reticulum vesicle protein 25
Gene namesi
Name:ERV25
Ordered Locus Names:YML012W
ORF Names:YM9571.06
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYML012w.
SGDiS000004473. ERV25.

Subcellular locationi

Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein
Note: Recycles between endoplasmic reticulum and Golgi.4 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 180160Lumenal Reviewed prediction
Add
BLAST
Transmembranei181 – 20121Helical; Reviewed prediction
Add
BLAST
Topological domaini202 – 21110Cytoplasmic Reviewed prediction

GO - Cellular componenti

  1. ER to Golgi transport vesicle Source: SGD
  2. Golgi membrane Source: UniProtKB-SubCell
  3. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  4. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi206 – 2083KTK → ATA: Decrease in COPI-binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 20202 Publications
Add
BLAST
Chaini21 – 211191Endoplasmic reticulum vesicle protein 25
PRO_0000010414Add
BLAST

Proteomic databases

PaxDbiP54837.
PeptideAtlasiP54837.
PRIDEiP54837.

Expressioni

Gene expression databases

GenevestigatoriP54837.

Interactioni

Subunit structurei

Associates with EMP24, ERP1 and ERP2.

Binary interactionsi

WithEntry#Exp.IntActNotes
EMP24P328033EBI-6642,EBI-6431
ERP1Q053596EBI-6642,EBI-6581
ERP5P388193EBI-6642,EBI-6603

Protein-protein interaction databases

BioGridi35157. 162 interactions.
DIPiDIP-2134N.
IntActiP54837. 66 interactions.
MINTiMINT-569840.
STRINGi4932.YML012W.

Structurei

3D structure databases

ProteinModelPortaliP54837.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini33 – 12189GOLD
Add
BLAST

Sequence similaritiesi

Belongs to the EMP24/GP25L family.
Contains 1 GOLD domain.

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG323830.
GeneTreeiENSGT00550000074954.
HOGENOMiHOG000160227.
OMAiVNEMEYL.
OrthoDBiEOG7RFTTD.

Family and domain databases

InterProiIPR009038. GOLD.
IPR015720. TMP21-related.
[Graphical view]
PANTHERiPTHR22811. PTHR22811. 1 hit.
PfamiPF01105. EMP24_GP25L. 1 hit.
[Graphical view]
PROSITEiPS50866. GOLD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P54837-1 [UniParc]FASTAAdd to Basket

« Hide

MQVLQLWLTT LISLVVAVQG LHFDIAASTD PEQVCIRDFV TEGQLVVADI    50
HSDGSVGDGQ KLNLFVRDSV GNEYRRKRDF AGDVRVAFTA PSSTAFDVCF 100
ENQAQYRGRS LSRAIELDIE SGAEARDWNK ISANEKLKPI EVELRRVEEI 150
TDEIVDELTY LKNREERLRD TNESTNRRVR NFSILVIIVL SSLGVWQVNY 200
LKNYFKTKHI I 211
Length:211
Mass (Da):24,106
Last modified:October 1, 1996 - v1
Checksum:i8E5ECE0336B1F885
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49810 Genomic DNA. Translation: CAA89940.1.
BK006946 Genomic DNA. Translation: DAA09886.1.
PIRiS55107.
RefSeqiNP_013701.1. NM_001182369.1.

Genome annotation databases

EnsemblFungiiYML012W; YML012W; YML012W.
GeneIDi854997.
KEGGisce:YML012W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49810 Genomic DNA. Translation: CAA89940.1 .
BK006946 Genomic DNA. Translation: DAA09886.1 .
PIRi S55107.
RefSeqi NP_013701.1. NM_001182369.1.

3D structure databases

ProteinModelPortali P54837.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35157. 162 interactions.
DIPi DIP-2134N.
IntActi P54837. 66 interactions.
MINTi MINT-569840.
STRINGi 4932.YML012W.

Proteomic databases

PaxDbi P54837.
PeptideAtlasi P54837.
PRIDEi P54837.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YML012W ; YML012W ; YML012W .
GeneIDi 854997.
KEGGi sce:YML012W.

Organism-specific databases

CYGDi YML012w.
SGDi S000004473. ERV25.

Phylogenomic databases

eggNOGi NOG323830.
GeneTreei ENSGT00550000074954.
HOGENOMi HOG000160227.
OMAi VNEMEYL.
OrthoDBi EOG7RFTTD.

Enzyme and pathway databases

BioCyci YEAST:G3O-32616-MONOMER.

Miscellaneous databases

NextBioi 978145.
PROi P54837.

Gene expression databases

Genevestigatori P54837.

Family and domain databases

InterProi IPR009038. GOLD.
IPR015720. TMP21-related.
[Graphical view ]
PANTHERi PTHR22811. PTHR22811. 1 hit.
Pfami PF01105. EMP24_GP25L. 1 hit.
[Graphical view ]
PROSITEi PS50866. GOLD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Erv25p, a component of COPII-coated vesicles, forms a complex with Emp24p that is required for efficient endoplasmic reticulum to Golgi transport."
    Belden W.J., Barlowe C.
    J. Biol. Chem. 271:26939-26946(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 21-36, FUNCTION, INTERACTION WITH EMP24, SUBCELLULAR LOCATION, ABSENCE OF GLYCOSYLATION.
  4. "Proteins in the early Golgi compartment of Saccharomyces cerevisiae immunoisolated by Sed5p."
    Cho J.-H., Noda Y., Yoda K.
    FEBS Lett. 469:151-154(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 21-25, SUBCELLULAR LOCATION.
  5. Cited for: INTERACTION WITH EMP24; ERP1 AND ERP2.
  6. "Distinct roles for the cytoplasmic tail sequences of Emp24p and Erv25p in transport between the endoplasmic reticulum and Golgi complex."
    Belden W.J., Barlowe C.
    J. Biol. Chem. 276:43040-43048(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF 206-LYS--LYS-208.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Immunoisolaton of the yeast Golgi subcompartments and characterization of a novel membrane protein, Svp26, discovered in the Sed5-containing compartments."
    Inadome H., Noda Y., Adachi H., Yoda K.
    Mol. Cell. Biol. 25:7696-7710(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiTMEDA_YEAST
AccessioniPrimary (citable) accession number: P54837
Secondary accession number(s): D6VZG2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 51700 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

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