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Protein

Endoplasmic reticulum vesicle protein 25

Gene

ERV25

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Constituent of COPII-coated endoplasmic reticulum-derived transport vesicles. Required for efficient transport of a subset of secretory proteins to the Golgi. Possesses a C-terminal dilysine motif that interacts with COPI coat subunits. Facilitates retrograde transport from the Golgi to the endoplasmic reticulum.2 Publications

GO - Biological processi

  1. ER to Golgi vesicle-mediated transport Source: SGD
  2. protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32616-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoplasmic reticulum vesicle protein 25
Gene namesi
Name:ERV25
Ordered Locus Names:YML012W
ORF Names:YM9571.06
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYML012w.
SGDiS000004473. ERV25.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 180160LumenalSequence AnalysisAdd
BLAST
Transmembranei181 – 20121HelicalSequence AnalysisAdd
BLAST
Topological domaini202 – 21110CytoplasmicSequence Analysis

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. ER to Golgi transport vesicle Source: SGD
  3. Golgi membrane Source: UniProtKB-SubCell
  4. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi206 – 2083KTK → ATA: Decrease in COPI-binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 20202 PublicationsAdd
BLAST
Chaini21 – 211191Endoplasmic reticulum vesicle protein 25PRO_0000010414Add
BLAST

Proteomic databases

PaxDbiP54837.
PeptideAtlasiP54837.
PRIDEiP54837.

Expressioni

Gene expression databases

GenevestigatoriP54837.

Interactioni

Subunit structurei

Associates with EMP24, ERP1 and ERP2.

Binary interactionsi

WithEntry#Exp.IntActNotes
EMP24P328033EBI-6642,EBI-6431
ERP1Q053596EBI-6642,EBI-6581
ERP5P388193EBI-6642,EBI-6603

Protein-protein interaction databases

BioGridi35157. 162 interactions.
DIPiDIP-2134N.
IntActiP54837. 66 interactions.
MINTiMINT-569840.
STRINGi4932.YML012W.

Structurei

3D structure databases

ProteinModelPortaliP54837.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini33 – 12189GOLDPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the EMP24/GP25L family.Curated
Contains 1 GOLD domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG323830.
GeneTreeiENSGT00550000074954.
HOGENOMiHOG000160227.
InParanoidiP54837.
OMAiDAIGNEY.
OrthoDBiEOG7RFTTD.

Family and domain databases

InterProiIPR009038. GOLD.
IPR015720. TMP21-related.
[Graphical view]
PANTHERiPTHR22811. PTHR22811. 1 hit.
PfamiPF01105. EMP24_GP25L. 1 hit.
[Graphical view]
PROSITEiPS50866. GOLD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P54837-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQVLQLWLTT LISLVVAVQG LHFDIAASTD PEQVCIRDFV TEGQLVVADI
60 70 80 90 100
HSDGSVGDGQ KLNLFVRDSV GNEYRRKRDF AGDVRVAFTA PSSTAFDVCF
110 120 130 140 150
ENQAQYRGRS LSRAIELDIE SGAEARDWNK ISANEKLKPI EVELRRVEEI
160 170 180 190 200
TDEIVDELTY LKNREERLRD TNESTNRRVR NFSILVIIVL SSLGVWQVNY
210
LKNYFKTKHI I
Length:211
Mass (Da):24,106
Last modified:October 1, 1996 - v1
Checksum:i8E5ECE0336B1F885
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49810 Genomic DNA. Translation: CAA89940.1.
BK006946 Genomic DNA. Translation: DAA09886.1.
PIRiS55107.
RefSeqiNP_013701.1. NM_001182369.1.

Genome annotation databases

EnsemblFungiiYML012W; YML012W; YML012W.
GeneIDi854997.
KEGGisce:YML012W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49810 Genomic DNA. Translation: CAA89940.1.
BK006946 Genomic DNA. Translation: DAA09886.1.
PIRiS55107.
RefSeqiNP_013701.1. NM_001182369.1.

3D structure databases

ProteinModelPortaliP54837.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35157. 162 interactions.
DIPiDIP-2134N.
IntActiP54837. 66 interactions.
MINTiMINT-569840.
STRINGi4932.YML012W.

Proteomic databases

PaxDbiP54837.
PeptideAtlasiP54837.
PRIDEiP54837.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYML012W; YML012W; YML012W.
GeneIDi854997.
KEGGisce:YML012W.

Organism-specific databases

CYGDiYML012w.
SGDiS000004473. ERV25.

Phylogenomic databases

eggNOGiNOG323830.
GeneTreeiENSGT00550000074954.
HOGENOMiHOG000160227.
InParanoidiP54837.
OMAiDAIGNEY.
OrthoDBiEOG7RFTTD.

Enzyme and pathway databases

BioCyciYEAST:G3O-32616-MONOMER.

Miscellaneous databases

NextBioi978145.
PROiP54837.

Gene expression databases

GenevestigatoriP54837.

Family and domain databases

InterProiIPR009038. GOLD.
IPR015720. TMP21-related.
[Graphical view]
PANTHERiPTHR22811. PTHR22811. 1 hit.
PfamiPF01105. EMP24_GP25L. 1 hit.
[Graphical view]
PROSITEiPS50866. GOLD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Erv25p, a component of COPII-coated vesicles, forms a complex with Emp24p that is required for efficient endoplasmic reticulum to Golgi transport."
    Belden W.J., Barlowe C.
    J. Biol. Chem. 271:26939-26946(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 21-36, FUNCTION, INTERACTION WITH EMP24, SUBCELLULAR LOCATION, ABSENCE OF GLYCOSYLATION.
  4. "Proteins in the early Golgi compartment of Saccharomyces cerevisiae immunoisolated by Sed5p."
    Cho J.-H., Noda Y., Yoda K.
    FEBS Lett. 469:151-154(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 21-25, SUBCELLULAR LOCATION.
  5. Cited for: INTERACTION WITH EMP24; ERP1 AND ERP2.
  6. "Distinct roles for the cytoplasmic tail sequences of Emp24p and Erv25p in transport between the endoplasmic reticulum and Golgi complex."
    Belden W.J., Barlowe C.
    J. Biol. Chem. 276:43040-43048(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF 206-LYS--LYS-208.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Immunoisolaton of the yeast Golgi subcompartments and characterization of a novel membrane protein, Svp26, discovered in the Sed5-containing compartments."
    Inadome H., Noda Y., Adachi H., Yoda K.
    Mol. Cell. Biol. 25:7696-7710(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiTMEDA_YEAST
AccessioniPrimary (citable) accession number: P54837
Secondary accession number(s): D6VZG2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 51700 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.