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P54837

- TMEDA_YEAST

UniProt

P54837 - TMEDA_YEAST

Protein

Endoplasmic reticulum vesicle protein 25

Gene

ERV25

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Constituent of COPII-coated endoplasmic reticulum-derived transport vesicles. Required for efficient transport of a subset of secretory proteins to the Golgi. Possesses a C-terminal dilysine motif that interacts with COPI coat subunits. Facilitates retrograde transport from the Golgi to the endoplasmic reticulum.2 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. ER to Golgi vesicle-mediated transport Source: SGD
    2. protein transport Source: UniProtKB-KW

    Keywords - Biological processi

    ER-Golgi transport, Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32616-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoplasmic reticulum vesicle protein 25
    Gene namesi
    Name:ERV25
    Ordered Locus Names:YML012W
    ORF Names:YM9571.06
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYML012w.
    SGDiS000004473. ERV25.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. ER to Golgi transport vesicle Source: SGD
    3. Golgi membrane Source: UniProtKB-SubCell
    4. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Golgi apparatus, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi206 – 2083KTK → ATA: Decrease in COPI-binding. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 20202 PublicationsAdd
    BLAST
    Chaini21 – 211191Endoplasmic reticulum vesicle protein 25PRO_0000010414Add
    BLAST

    Proteomic databases

    PaxDbiP54837.
    PeptideAtlasiP54837.
    PRIDEiP54837.

    Expressioni

    Gene expression databases

    GenevestigatoriP54837.

    Interactioni

    Subunit structurei

    Associates with EMP24, ERP1 and ERP2.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    EMP24P328033EBI-6642,EBI-6431
    ERP1Q053596EBI-6642,EBI-6581
    ERP5P388193EBI-6642,EBI-6603

    Protein-protein interaction databases

    BioGridi35157. 162 interactions.
    DIPiDIP-2134N.
    IntActiP54837. 66 interactions.
    MINTiMINT-569840.
    STRINGi4932.YML012W.

    Structurei

    3D structure databases

    ProteinModelPortaliP54837.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini21 – 180160LumenalSequence AnalysisAdd
    BLAST
    Topological domaini202 – 21110CytoplasmicSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei181 – 20121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini33 – 12189GOLDPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the EMP24/GP25L family.Curated
    Contains 1 GOLD domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG323830.
    GeneTreeiENSGT00550000074954.
    HOGENOMiHOG000160227.
    OMAiVNEMEYL.
    OrthoDBiEOG7RFTTD.

    Family and domain databases

    InterProiIPR009038. GOLD.
    IPR015720. TMP21-related.
    [Graphical view]
    PANTHERiPTHR22811. PTHR22811. 1 hit.
    PfamiPF01105. EMP24_GP25L. 1 hit.
    [Graphical view]
    PROSITEiPS50866. GOLD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P54837-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQVLQLWLTT LISLVVAVQG LHFDIAASTD PEQVCIRDFV TEGQLVVADI    50
    HSDGSVGDGQ KLNLFVRDSV GNEYRRKRDF AGDVRVAFTA PSSTAFDVCF 100
    ENQAQYRGRS LSRAIELDIE SGAEARDWNK ISANEKLKPI EVELRRVEEI 150
    TDEIVDELTY LKNREERLRD TNESTNRRVR NFSILVIIVL SSLGVWQVNY 200
    LKNYFKTKHI I 211
    Length:211
    Mass (Da):24,106
    Last modified:October 1, 1996 - v1
    Checksum:i8E5ECE0336B1F885
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49810 Genomic DNA. Translation: CAA89940.1.
    BK006946 Genomic DNA. Translation: DAA09886.1.
    PIRiS55107.
    RefSeqiNP_013701.1. NM_001182369.1.

    Genome annotation databases

    EnsemblFungiiYML012W; YML012W; YML012W.
    GeneIDi854997.
    KEGGisce:YML012W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49810 Genomic DNA. Translation: CAA89940.1 .
    BK006946 Genomic DNA. Translation: DAA09886.1 .
    PIRi S55107.
    RefSeqi NP_013701.1. NM_001182369.1.

    3D structure databases

    ProteinModelPortali P54837.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35157. 162 interactions.
    DIPi DIP-2134N.
    IntActi P54837. 66 interactions.
    MINTi MINT-569840.
    STRINGi 4932.YML012W.

    Proteomic databases

    PaxDbi P54837.
    PeptideAtlasi P54837.
    PRIDEi P54837.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YML012W ; YML012W ; YML012W .
    GeneIDi 854997.
    KEGGi sce:YML012W.

    Organism-specific databases

    CYGDi YML012w.
    SGDi S000004473. ERV25.

    Phylogenomic databases

    eggNOGi NOG323830.
    GeneTreei ENSGT00550000074954.
    HOGENOMi HOG000160227.
    OMAi VNEMEYL.
    OrthoDBi EOG7RFTTD.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32616-MONOMER.

    Miscellaneous databases

    NextBioi 978145.
    PROi P54837.

    Gene expression databases

    Genevestigatori P54837.

    Family and domain databases

    InterProi IPR009038. GOLD.
    IPR015720. TMP21-related.
    [Graphical view ]
    PANTHERi PTHR22811. PTHR22811. 1 hit.
    Pfami PF01105. EMP24_GP25L. 1 hit.
    [Graphical view ]
    PROSITEi PS50866. GOLD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Erv25p, a component of COPII-coated vesicles, forms a complex with Emp24p that is required for efficient endoplasmic reticulum to Golgi transport."
      Belden W.J., Barlowe C.
      J. Biol. Chem. 271:26939-26946(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 21-36, FUNCTION, INTERACTION WITH EMP24, SUBCELLULAR LOCATION, ABSENCE OF GLYCOSYLATION.
    4. "Proteins in the early Golgi compartment of Saccharomyces cerevisiae immunoisolated by Sed5p."
      Cho J.-H., Noda Y., Yoda K.
      FEBS Lett. 469:151-154(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 21-25, SUBCELLULAR LOCATION.
    5. Cited for: INTERACTION WITH EMP24; ERP1 AND ERP2.
    6. "Distinct roles for the cytoplasmic tail sequences of Emp24p and Erv25p in transport between the endoplasmic reticulum and Golgi complex."
      Belden W.J., Barlowe C.
      J. Biol. Chem. 276:43040-43048(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF 206-LYS--LYS-208.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Immunoisolaton of the yeast Golgi subcompartments and characterization of a novel membrane protein, Svp26, discovered in the Sed5-containing compartments."
      Inadome H., Noda Y., Adachi H., Yoda K.
      Mol. Cell. Biol. 25:7696-7710(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiTMEDA_YEAST
    AccessioniPrimary (citable) accession number: P54837
    Secondary accession number(s): D6VZG2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 51700 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3