P54837 (TMEDA_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoplasmic reticulum vesicle protein 25

Gene names

Name:	ERV25
Ordered Locus Names:	YML012W
ORF Names:	YM9571.06

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	211 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Constituent of COPII-coated endoplasmic reticulum-derived transport vesicles. Required for efficient transport of a subset of secretory proteins to the Golgi. Possesses a C-terminal dilysine motif that interacts with COPI coat subunits. Facilitates retrograde transport from the Golgi to the endoplasmic reticulum. Ref.3 Ref.6
Subunit structure	Associates with EMP24, ERP1 and ERP2.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Note: Recycles between endoplasmic reticulum and Golgi. Ref.3 Ref.4 Ref.6 Ref.8
Miscellaneous	Present with 51700 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the EMP24/GP25L family. Contains 1 GOLD domain.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane
Domain	Signal Transmembrane Transmembrane helix
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	ER to Golgi vesicle-mediated transport Inferred from physical interaction. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ER to Golgi transport vesicle Inferred from direct assay. Source: SGD Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ERP1	Q05359	6	EBI-6642,EBI-6581
ERP2	P39704	4	EBI-6642,EBI-6587
ERP3	Q12403	3	EBI-6642,EBI-6593

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Ref.3 Ref.4
	Chain	21 – 211	191	Endoplasmic reticulum vesicle protein 25		PRO_0000010414
Regions
	Topological domain	21 – 180	160	Lumenal Potential
	Transmembrane	181 – 201	21	Helical; Potential
	Topological domain	202 – 211	10	Cytoplasmic Potential
	Domain	33 – 121	89	GOLD
Experimental info
	Mutagenesis	206 – 208	3	KTK → ATA: Decrease in COPI-binding. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P54837 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 8E5ECE0336B1F885	FASTA	211	24,106
	10 20 30 40 50 60 MQVLQLWLTT LISLVVAVQG LHFDIAASTD PEQVCIRDFV TEGQLVVADI HSDGSVGDGQ 70 80 90 100 110 120 KLNLFVRDSV GNEYRRKRDF AGDVRVAFTA PSSTAFDVCF ENQAQYRGRS LSRAIELDIE 130 140 150 160 170 180 SGAEARDWNK ISANEKLKPI EVELRRVEEI TDEIVDELTY LKNREERLRD TNESTNRRVR 190 200 210 NFSILVIIVL SSLGVWQVNY LKNYFKTKHI I

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Erv25p, a component of COPII-coated vesicles, forms a complex with Emp24p that is required for efficient endoplasmic reticulum to Golgi transport." Belden W.J., Barlowe C. J. Biol. Chem. 271:26939-26946(1996) [PubMed: 8900179] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-36, FUNCTION, INTERACTION WITH EMP24, SUBCELLULAR LOCATION, ABSENCE OF GLYCOSYLATION.
[4]	"Proteins in the early Golgi compartment of Saccharomyces cerevisiae immunoisolated by Sed5p." Cho J.-H., Noda Y., Yoda K. FEBS Lett. 469:151-154(2000) [PubMed: 10713261] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-25, SUBCELLULAR LOCATION.
[5]	"Erp1p and Erp2p, partners for Emp24p and Erv25p in a yeast p24 complex." Marzioch M., Henthorn D.C., Herrmann J.M., Wilson R., Thomas D.Y., Bergeron J.J.M., Solari R.C., Rowley A. Mol. Biol. Cell 10:1923-1938(1999) [PubMed: 10359606] [Abstract] Cited for: INTERACTION WITH EMP24; ERP1 AND ERP2.
[6]	"Distinct roles for the cytoplasmic tail sequences of Emp24p and Erv25p in transport between the endoplasmic reticulum and Golgi complex." Belden W.J., Barlowe C. J. Biol. Chem. 276:43040-43048(2001) [PubMed: 11560939] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF 206-LYS--LYS-208.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Immunoisolaton of the yeast Golgi subcompartments and characterization of a novel membrane protein, Svp26, discovered in the Sed5-containing compartments." Inadome H., Noda Y., Adachi H., Yoda K. Mol. Cell. Biol. 25:7696-7710(2005) [PubMed: 16107716] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49810 Genomic DNA. Translation: CAA89940.1. BK006946 Genomic DNA. Translation: DAA09886.1.
PIR	S55107.
RefSeq	NP_013701.1. NM_001182369.1.
3D structure databases
ProteinModelPortal	P54837.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2134N.
IntAct	P54837. 113 interactions.
MINT	MINT-569840.
STRING	P54837.
Proteomic databases
PeptideAtlas	P54837.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML012W; YML012W; YML012W.
GeneID	854997.
KEGG	sce:YML012W.
NMPDR	fig|4932.3.peg.4742.
Organism-specific databases
CYGD	YML012w.
SGD	S000004473. ERV25.
Phylogenomic databases
eggNOG	fuNOG04308.
GeneTree	EFGT00070000008733.
HOGENOM	HBG559015.
OMA	NERVKWF.
OrthoDB	EOG47H90K.
Gene expression databases
ArrayExpress	P54837.
Genevestigator	P54837.
GermOnline	YML012W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR009038. GOLD. [Graphical view]
Pfam	PF01105. EMP24_GP25L. 1 hit. [Graphical view]
PROSITE	PS50866. GOLD. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978145.

Entry information

Entry name

TMEDA_YEAST

Accession

Primary (citable) accession number: P54837
Secondary accession number(s): D6VZG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program