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Reviewed, UniProtKB/Swiss-Prot P54837 (ERV25_YEAST)

Last modified November 24, 2009. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Endoplasmic reticulum vesicle protein 25
Gene names
Name: ERV25
Ordered Locus Names: YML012W
ORF Names: YM9571.06
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length211 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Constituent of COPII-coated endoplasmic reticulum-derived transport vesicles. Required for efficient transport of a subset of secretory proteins to the Golgi. Possesses a C-terminal dilysine motif that interacts with COPI coat subunits. Facilitates retrograde transport from the Golgi to the endoplasmic reticulum. Ref.2 Ref.5

Subunit structure

Associates with EMP24, ERP1 and ERP2.

Subcellular location

Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Note: Recycles between endoplasmic reticulum and Golgi. Ref.2 Ref.5 Ref.3 Ref.7

Miscellaneous

Present with 51700 molecules/cell in log phase SD medium. Ref.6

Sequence similarities

Belongs to the EMP24/GP25L family.

Contains 1 GOLD domain.

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself1EBI-6642,EBI-6642
P381421EBI-6642,EBI-21079
P386941EBI-6642,EBI-24511
P388451EBI-6642,EBI-24770
Q121441EBI-6642,EBI-37691
Q121551EBI-6642,EBI-30729
Q121641EBI-6642,EBI-30000
Q127461EBI-6642,EBI-27973
AGP1P253761EBI-6642,EBI-2357
AIM27P360391EBI-6642,EBI-27005
ALG5P403501EBI-6642,EBI-2470
ARF1P110761EBI-6642,EBI-2816
BAP2P380841EBI-6642,EBI-3414
CAN1P048171EBI-6642,EBI-3993
CBR1P386261EBI-6642,EBI-2048224
CHO1P084561EBI-6642,EBI-14055
COS4P435421EBI-6642,EBI-2050225
CPT1P178981EBI-6642,EBI-2050738
CSG2P352061EBI-6642,EBI-2051140
DAL5P153651EBI-6642,EBI-5527
ELO1P395401EBI-6642,EBI-2049096
ELO2P253581EBI-6642,EBI-6415
EMC2P471331EBI-6642,EBI-25568
EMP47P435551EBI-6642,EBI-6439
EPT1P221401EBI-6642,EBI-6494
ERG1P324761EBI-6642,EBI-6545
ERG11P106141EBI-6642,EBI-5127
ERG25P530451EBI-6642,EBI-6506
ERG26P531991EBI-6642,EBI-6514
ERG3P323531EBI-6642,EBI-6554
ERG5P547811EBI-6642,EBI-6563
ERP1Q053591EBI-6642,EBI-6581
ERP2P397041EBI-6642,EBI-6587
ERP3Q124031EBI-6642,EBI-6593
ERP4Q124501EBI-6642,EBI-6598
ERP5P388191EBI-6642,EBI-6603
ERP6P531981EBI-6642,EBI-2058104
ERV29P533371EBI-6642,EBI-23662
GAP1P191451EBI-6642,EBI-7314
GNP1P488131EBI-6642,EBI-7758
GOT1Q035541EBI-6642,EBI-2057821
GRX2P176951EBI-6642,EBI-7699
GSF2Q046971EBI-6642,EBI-27807
GTT1P405821EBI-6642,EBI-7941
GWT1P470261EBI-6642,EBI-25989
HXT3P324661EBI-6642,EBI-8770
HXT7P390041EBI-6642,EBI-8790
ICE2P404991EBI-6642,EBI-25109
IST2P382501EBI-6642,EBI-21520
KRE27P405401EBI-6642,EBI-24977
MCD4P360511EBI-6642,EBI-2070979
NSG2P538981EBI-6642,EBI-2048508
OLE1P211471EBI-6642,EBI-2098
ORM1P532241EBI-6642,EBI-12592
ORM2Q061441EBI-6642,EBI-34916
OST1P415431EBI-6642,EBI-12651
PHO84P252971EBI-6642,EBI-13320
PHO88P382641EBI-6642,EBI-13350
PHS1P408571EBI-6642,EBI-26003
PIS1P061971EBI-6642,EBI-13458
PKR1Q038801EBI-6642,EBI-27252
PMP2P409751EBI-6642,EBI-2043041
PMP3P872841EBI-6642,EBI-13555
PMR1P135861EBI-6642,EBI-3091
PPZ1P265701EBI-6642,EBI-13807
RBD2Q122701EBI-6642,EBI-31471
RTN1Q049471EBI-6642,EBI-38020
SAC1P323681EBI-6642,EBI-16210
SAM3Q089861EBI-6642,EBI-2056826
SCS7Q035291EBI-6642,EBI-16747
SEC61P329151EBI-6642,EBI-16400
SEC63P149061EBI-6642,EBI-16636
SEY1Q992871EBI-6642,EBI-37523
SHR3Q027741EBI-6642,EBI-17099
SPF1P399861EBI-6642,EBI-3128
SSH1P383531EBI-6642,EBI-18175
SUR2P389921EBI-6642,EBI-18574
SVP26P388691EBI-6642,EBI-24844
SWP1Q027951EBI-6642,EBI-12666
TNA1P533221EBI-6642,EBI-2051164
UBA4P388201EBI-6642,EBI-24676
UIP4Q089261EBI-6642,EBI-2044051
VHS3Q084381EBI-6642,EBI-30482
VMA21P418061EBI-6642,EBI-20323
VRG4P401071EBI-6642,EBI-7764
WBP1P337671EBI-6642,EBI-12658
YDC1Q028961EBI-6642,EBI-22203
YDR476CQ033621EBI-6642,EBI-2057915
YER053C-AQ3E7B01EBI-6642,EBI-2047907
YET1P357231EBI-6642,EBI-26730
YOP1Q124021EBI-6642,EBI-37092

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Ref.2 Ref.3
Chain21 – 211191Endoplasmic reticulum vesicle protein 25
PRO_0000010414

Regions

Topological domain21 – 180160Lumenal Potential
Transmembrane181 – 20121 Potential
Topological domain202 – 21110Cytoplasmic Potential
Domain33 – 12189GOLD

Experimental info

Mutagenesis206 – 2083KTK → ATA: Decrease in COPI-binding. Ref.5

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Sequences

Sequence LengthMass (Da)Tools
P54837-1 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 8E5ECE0336B1F885

FASTA21124,106
        10         20         30         40         50         60 
MQVLQLWLTT LISLVVAVQG LHFDIAASTD PEQVCIRDFV TEGQLVVADI HSDGSVGDGQ 

        70         80         90        100        110        120 
KLNLFVRDSV GNEYRRKRDF AGDVRVAFTA PSSTAFDVCF ENQAQYRGRS LSRAIELDIE 

       130        140        150        160        170        180 
SGAEARDWNK ISANEKLKPI EVELRRVEEI TDEIVDELTY LKNREERLRD TNESTNRRVR 

       190        200        210 
NFSILVIIVL SSLGVWQVNY LKNYFKTKHI I

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References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed: 9169872] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]"Erv25p, a component of COPII-coated vesicles, forms a complex with Emp24p that is required for efficient endoplasmic reticulum to Golgi transport."
Belden W.J., Barlowe C.
J. Biol. Chem. 271:26939-26946(1996) [PubMed: 8900179] [Abstract]
Cited for: PROTEIN SEQUENCE OF 21-36, FUNCTION, INTERACTION WITH EMP24, SUBCELLULAR LOCATION, ABSENCE OF GLYCOSYLATION.
[3]"Proteins in the early Golgi compartment of Saccharomyces cerevisiae immunoisolated by Sed5p."
Cho J.-H., Noda Y., Yoda K.
FEBS Lett. 469:151-154(2000) [PubMed: 10713261] [Abstract]
Cited for: PROTEIN SEQUENCE OF 21-25, SUBCELLULAR LOCATION.
[4]"Erp1p and Erp2p, partners for Emp24p and Erv25p in a yeast p24 complex."
Marzioch M., Henthorn D.C., Herrmann J.M., Wilson R., Thomas D.Y., Bergeron J.J.M., Solari R.C., Rowley A.
Mol. Biol. Cell 10:1923-1938(1999) [PubMed: 10359606] [Abstract]
Cited for: INTERACTION WITH EMP24; ERP1 AND ERP2.
[5]"Distinct roles for the cytoplasmic tail sequences of Emp24p and Erv25p in transport between the endoplasmic reticulum and Golgi complex."
Belden W.J., Barlowe C.
J. Biol. Chem. 276:43040-43048(2001) [PubMed: 11560939] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF 206-LYS--LYS-208.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Immunoisolaton of the yeast Golgi subcompartments and characterization of a novel membrane protein, Svp26, discovered in the Sed5-containing compartments."
Inadome H., Noda Y., Adachi H., Yoda K.
Mol. Cell. Biol. 25:7696-7710(2005) [PubMed: 16107716] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

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Cross-references

Sequence databases

Z49810 Genomic DNA. Translation: CAA89940.1.
PIRS55107.
RefSeqNP_013701.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:2134N.
IntActP54837. 118 interactions.
STRINGP54837.

Proteomic databases

PeptideAtlasP54837.

Genome annotation databases

EnsemblYML012W; YML012W; YML012W; Saccharomyces cerevisiae. [Genome view]
GeneID854997.
KEGGsce:YML012W.
NMPDRfig|4932.3.peg.4742.

Organism-specific databases

CYGDYML012w.
SGDS000004473. ERV25.

Phylogenomic databases

HOGENOMP54837.
OMALESGHTK
OrthoDBEOG9QJT4T

Gene expression databases

ArrayExpressP54837.
GenevestigatorP54837.
GermOnlineYML012W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR000348. Emp24_gp25L_p24.
IPR009038. GOLD.
[Graphical view]
PfamPF01105. EMP24_GP25L. 1 hit.
[Graphical view]
PROSITEPS50866. GOLD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio978145.

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Entry information

Entry nameERV25_YEAST
AccessionPrimary (citable) accession number: P54837
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 24, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents