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Protein

Endoplasmic reticulum vesicle protein 25

Gene

ERV25

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Constituent of COPII-coated endoplasmic reticulum-derived transport vesicles. Required for efficient transport of a subset of secretory proteins to the Golgi. Possesses a C-terminal dilysine motif that interacts with COPI coat subunits. Facilitates retrograde transport from the Golgi to the endoplasmic reticulum.2 Publications

Miscellaneous

Present with 51700 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

  • ER to Golgi vesicle-mediated transport Source: SGD
  • protein transport Source: UniProtKB-KW

Keywordsi

Biological processER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32616-MONOMER.
ReactomeiR-SCE-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoplasmic reticulum vesicle protein 25
Gene namesi
Name:ERV25
Ordered Locus Names:YML012W
ORF Names:YM9571.06
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML012W.
SGDiS000004473. ERV25.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 180LumenalSequence analysisAdd BLAST160
Transmembranei181 – 201HelicalSequence analysisAdd BLAST21
Topological domaini202 – 211CytoplasmicSequence analysis10

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi206 – 208KTK → ATA: Decrease in COPI-binding. 1 Publication3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 202 PublicationsAdd BLAST20
ChainiPRO_000001041421 – 211Endoplasmic reticulum vesicle protein 25Add BLAST191

Proteomic databases

PRIDEiP54837.

Interactioni

Subunit structurei

Associates with EMP24, ERP1 and ERP2.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35157. 190 interactors.
DIPiDIP-2134N.
IntActiP54837. 66 interactors.
MINTiMINT-569840.
STRINGi4932.YML012W.

Structurei

3D structure databases

ProteinModelPortaliP54837.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 121GOLDPROSITE-ProRule annotationAdd BLAST89

Sequence similaritiesi

Belongs to the EMP24/GP25L family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000074954.
HOGENOMiHOG000160227.
InParanoidiP54837.
KOiK20352.
OMAiFSMCCLI.
OrthoDBiEOG092C4SOM.

Family and domain databases

InterProiView protein in InterPro
IPR009038. GOLD_dom.
IPR015720. TMP21-related.
PANTHERiPTHR22811. PTHR22811. 1 hit.
PfamiView protein in Pfam
PF01105. EMP24_GP25L. 1 hit.
SMARTiView protein in SMART
SM01190. EMP24_GP25L. 1 hit.
PROSITEiView protein in PROSITE
PS50866. GOLD. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P54837-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQVLQLWLTT LISLVVAVQG LHFDIAASTD PEQVCIRDFV TEGQLVVADI
60 70 80 90 100
HSDGSVGDGQ KLNLFVRDSV GNEYRRKRDF AGDVRVAFTA PSSTAFDVCF
110 120 130 140 150
ENQAQYRGRS LSRAIELDIE SGAEARDWNK ISANEKLKPI EVELRRVEEI
160 170 180 190 200
TDEIVDELTY LKNREERLRD TNESTNRRVR NFSILVIIVL SSLGVWQVNY
210
LKNYFKTKHI I
Length:211
Mass (Da):24,106
Last modified:October 1, 1996 - v1
Checksum:i8E5ECE0336B1F885
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49810 Genomic DNA. Translation: CAA89940.1.
BK006946 Genomic DNA. Translation: DAA09886.1.
PIRiS55107.
RefSeqiNP_013701.1. NM_001182369.1.

Genome annotation databases

EnsemblFungiiYML012W; YML012W; YML012W.
GeneIDi854997.
KEGGisce:YML012W.

Similar proteinsi

Entry informationi

Entry nameiTMEDA_YEAST
AccessioniPrimary (citable) accession number: P54837
Secondary accession number(s): D6VZG2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 25, 2017
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names