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Protein

Endoplasmic reticulum vesicle protein 25

Gene

ERV25

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Constituent of COPII-coated endoplasmic reticulum-derived transport vesicles. Required for efficient transport of a subset of secretory proteins to the Golgi. Possesses a C-terminal dilysine motif that interacts with COPI coat subunits. Facilitates retrograde transport from the Golgi to the endoplasmic reticulum.2 Publications

GO - Biological processi

  • ER to Golgi vesicle-mediated transport Source: SGD
  • protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32616-MONOMER.
ReactomeiR-SCE-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoplasmic reticulum vesicle protein 25
Gene namesi
Name:ERV25
Ordered Locus Names:YML012W
ORF Names:YM9571.06
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML012W.
SGDiS000004473. ERV25.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 180LumenalSequence analysisAdd BLAST160
Transmembranei181 – 201HelicalSequence analysisAdd BLAST21
Topological domaini202 – 211CytoplasmicSequence analysis10

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi206 – 208KTK → ATA: Decrease in COPI-binding. 1 Publication3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 202 PublicationsAdd BLAST20
ChainiPRO_000001041421 – 211Endoplasmic reticulum vesicle protein 25Add BLAST191

Proteomic databases

PRIDEiP54837.

Interactioni

Subunit structurei

Associates with EMP24, ERP1 and ERP2.

Binary interactionsi

WithEntry#Exp.IntActNotes
EMP24P328033EBI-6642,EBI-6431
ERP1Q053596EBI-6642,EBI-6581
ERP5P388193EBI-6642,EBI-6603

Protein-protein interaction databases

BioGridi35157. 169 interactors.
DIPiDIP-2134N.
IntActiP54837. 66 interactors.
MINTiMINT-569840.

Structurei

3D structure databases

ProteinModelPortaliP54837.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 121GOLDPROSITE-ProRule annotationAdd BLAST89

Sequence similaritiesi

Belongs to the EMP24/GP25L family.Curated
Contains 1 GOLD domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000074954.
HOGENOMiHOG000160227.
InParanoidiP54837.
KOiK20352.
OMAiNTRVKWF.
OrthoDBiEOG092C4SOM.

Family and domain databases

InterProiIPR009038. GOLD_dom.
IPR015720. TMP21-related.
[Graphical view]
PANTHERiPTHR22811. PTHR22811. 1 hit.
PfamiPF01105. EMP24_GP25L. 1 hit.
[Graphical view]
SMARTiSM01190. EMP24_GP25L. 1 hit.
[Graphical view]
PROSITEiPS50866. GOLD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P54837-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQVLQLWLTT LISLVVAVQG LHFDIAASTD PEQVCIRDFV TEGQLVVADI
60 70 80 90 100
HSDGSVGDGQ KLNLFVRDSV GNEYRRKRDF AGDVRVAFTA PSSTAFDVCF
110 120 130 140 150
ENQAQYRGRS LSRAIELDIE SGAEARDWNK ISANEKLKPI EVELRRVEEI
160 170 180 190 200
TDEIVDELTY LKNREERLRD TNESTNRRVR NFSILVIIVL SSLGVWQVNY
210
LKNYFKTKHI I
Length:211
Mass (Da):24,106
Last modified:October 1, 1996 - v1
Checksum:i8E5ECE0336B1F885
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49810 Genomic DNA. Translation: CAA89940.1.
BK006946 Genomic DNA. Translation: DAA09886.1.
PIRiS55107.
RefSeqiNP_013701.1. NM_001182369.1.

Genome annotation databases

EnsemblFungiiYML012W; YML012W; YML012W.
GeneIDi854997.
KEGGisce:YML012W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49810 Genomic DNA. Translation: CAA89940.1.
BK006946 Genomic DNA. Translation: DAA09886.1.
PIRiS55107.
RefSeqiNP_013701.1. NM_001182369.1.

3D structure databases

ProteinModelPortaliP54837.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35157. 169 interactors.
DIPiDIP-2134N.
IntActiP54837. 66 interactors.
MINTiMINT-569840.

Proteomic databases

PRIDEiP54837.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYML012W; YML012W; YML012W.
GeneIDi854997.
KEGGisce:YML012W.

Organism-specific databases

EuPathDBiFungiDB:YML012W.
SGDiS000004473. ERV25.

Phylogenomic databases

GeneTreeiENSGT00550000074954.
HOGENOMiHOG000160227.
InParanoidiP54837.
KOiK20352.
OMAiNTRVKWF.
OrthoDBiEOG092C4SOM.

Enzyme and pathway databases

BioCyciYEAST:G3O-32616-MONOMER.
ReactomeiR-SCE-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Miscellaneous databases

PROiP54837.

Family and domain databases

InterProiIPR009038. GOLD_dom.
IPR015720. TMP21-related.
[Graphical view]
PANTHERiPTHR22811. PTHR22811. 1 hit.
PfamiPF01105. EMP24_GP25L. 1 hit.
[Graphical view]
SMARTiSM01190. EMP24_GP25L. 1 hit.
[Graphical view]
PROSITEiPS50866. GOLD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTMEDA_YEAST
AccessioniPrimary (citable) accession number: P54837
Secondary accession number(s): D6VZG2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 51700 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.