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P54837 (TMEDA_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoplasmic reticulum vesicle protein 25
Gene names
Name:ERV25
Ordered Locus Names:YML012W
ORF Names:YM9571.06
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length211 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Constituent of COPII-coated endoplasmic reticulum-derived transport vesicles. Required for efficient transport of a subset of secretory proteins to the Golgi. Possesses a C-terminal dilysine motif that interacts with COPI coat subunits. Facilitates retrograde transport from the Golgi to the endoplasmic reticulum. Ref.3 Ref.6

Subunit structure

Associates with EMP24, ERP1 and ERP2.

Subcellular location

Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Note: Recycles between endoplasmic reticulum and Golgi. Ref.3 Ref.4 Ref.6 Ref.8

Miscellaneous

Present with 51700 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the EMP24/GP25L family.

Contains 1 GOLD domain.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Ref.3 Ref.4
Chain21 – 211191Endoplasmic reticulum vesicle protein 25
PRO_0000010414

Regions

Topological domain21 – 180160Lumenal Potential
Transmembrane181 – 20121Helical; Potential
Topological domain202 – 21110Cytoplasmic Potential
Domain33 – 12189GOLD

Experimental info

Mutagenesis206 – 2083KTK → ATA: Decrease in COPI-binding. Ref.6

Sequences

Sequence LengthMass (Da)Tools
P54837 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 8E5ECE0336B1F885

FASTA21124,106
        10         20         30         40         50         60 
MQVLQLWLTT LISLVVAVQG LHFDIAASTD PEQVCIRDFV TEGQLVVADI HSDGSVGDGQ 

        70         80         90        100        110        120 
KLNLFVRDSV GNEYRRKRDF AGDVRVAFTA PSSTAFDVCF ENQAQYRGRS LSRAIELDIE 

       130        140        150        160        170        180 
SGAEARDWNK ISANEKLKPI EVELRRVEEI TDEIVDELTY LKNREERLRD TNESTNRRVR 

       190        200        210 
NFSILVIIVL SSLGVWQVNY LKNYFKTKHI I 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Erv25p, a component of COPII-coated vesicles, forms a complex with Emp24p that is required for efficient endoplasmic reticulum to Golgi transport."
Belden W.J., Barlowe C.
J. Biol. Chem. 271:26939-26946(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 21-36, FUNCTION, INTERACTION WITH EMP24, SUBCELLULAR LOCATION, ABSENCE OF GLYCOSYLATION.
[4]"Proteins in the early Golgi compartment of Saccharomyces cerevisiae immunoisolated by Sed5p."
Cho J.-H., Noda Y., Yoda K.
FEBS Lett. 469:151-154(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 21-25, SUBCELLULAR LOCATION.
[5]"Erp1p and Erp2p, partners for Emp24p and Erv25p in a yeast p24 complex."
Marzioch M., Henthorn D.C., Herrmann J.M., Wilson R., Thomas D.Y., Bergeron J.J.M., Solari R.C., Rowley A.
Mol. Biol. Cell 10:1923-1938(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH EMP24; ERP1 AND ERP2.
[6]"Distinct roles for the cytoplasmic tail sequences of Emp24p and Erv25p in transport between the endoplasmic reticulum and Golgi complex."
Belden W.J., Barlowe C.
J. Biol. Chem. 276:43040-43048(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF 206-LYS--LYS-208.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Immunoisolaton of the yeast Golgi subcompartments and characterization of a novel membrane protein, Svp26, discovered in the Sed5-containing compartments."
Inadome H., Noda Y., Adachi H., Yoda K.
Mol. Cell. Biol. 25:7696-7710(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49810 Genomic DNA. Translation: CAA89940.1.
BK006946 Genomic DNA. Translation: DAA09886.1.
PIRS55107.
RefSeqNP_013701.1. NM_001182369.1.

3D structure databases

ProteinModelPortalP54837.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35157. 161 interactions.
DIPDIP-2134N.
IntActP54837. 66 interactions.
MINTMINT-569840.
STRING4932.YML012W.

Proteomic databases

PaxDbP54837.
PeptideAtlasP54837.
PRIDEP54837.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYML012W; YML012W; YML012W.
GeneID854997.
KEGGsce:YML012W.

Organism-specific databases

CYGDYML012w.
SGDS000004473. ERV25.

Phylogenomic databases

eggNOGNOG323830.
GeneTreeENSGT00550000074954.
HOGENOMHOG000160227.
OMAVNEMEYL.
OrthoDBEOG7RFTTD.

Enzyme and pathway databases

BioCycYEAST:G3O-32616-MONOMER.

Gene expression databases

GenevestigatorP54837.

Family and domain databases

InterProIPR009038. GOLD.
IPR015720. TMP21-related.
[Graphical view]
PANTHERPTHR22811. PTHR22811. 1 hit.
PfamPF01105. EMP24_GP25L. 1 hit.
[Graphical view]
PROSITEPS50866. GOLD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio978145.
PROP54837.

Entry information

Entry nameTMEDA_YEAST
AccessionPrimary (citable) accession number: P54837
Secondary accession number(s): D6VZG2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families