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Protein

Tyrosinase

Gene

TYR

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine. In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone.By similarity

Catalytic activityi

2 L-dopa + O2 = 2 dopaquinone + 2 H2O.By similarity
L-tyrosine + O2 = dopaquinone + H2O.By similarity

Cofactori

Cu2+By similarityNote: Binds 2 copper ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi180Copper ABy similarity1
Metal bindingi202Copper ABy similarity1
Metal bindingi211Copper ABy similarity1
Metal bindingi363Copper BBy similarity1
Metal bindingi367Copper BBy similarity1
Metal bindingi390Copper BBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Melanin biosynthesis

Keywords - Ligandi

Copper, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosinase (EC:1.14.18.1)
Alternative name(s):
Monophenol monooxygenase
Gene namesi
Name:TYR
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Unplaced

Subcellular locationi

  • Melanosome membrane By similarity; Single-pass type I membrane protein By similarity
  • Melanosome By similarity

  • Note: Proper trafficking to melanosome is regulated by SGSM2, ANKRD27, RAB9A, RAB32 and RAB38.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini19 – 473Lumenal, melanosomeSequence analysisAdd BLAST455
Transmembranei474 – 494HelicalSequence analysisAdd BLAST21
Topological domaini495 – 530CytoplasmicSequence analysisAdd BLAST36

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000003587719 – 530TyrosinaseAdd BLAST512

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi86N-linked (GlcNAc...)Sequence analysis1
Glycosylationi111N-linked (GlcNAc...)Sequence analysis1
Glycosylationi161N-linked (GlcNAc...)Sequence analysis1
Glycosylationi230N-linked (GlcNAc...)Sequence analysis1
Glycosylationi337N-linked (GlcNAc...)Sequence analysis1
Glycosylationi371N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Glycosylated.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP54834.

Interactioni

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000006504.

Structurei

3D structure databases

ProteinModelPortaliP54834.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tyrosinase family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IEZU. Eukaryota.
ENOG410Y42I. LUCA.
HOGENOMiHOG000118376.
HOVERGENiHBG003553.
InParanoidiP54834.
KOiK00505.

Family and domain databases

Gene3Di1.10.1280.10. 1 hit.
InterProiIPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view]
PfamiPF00264. Tyrosinase. 1 hit.
[Graphical view]
PRINTSiPR00092. TYROSINASE.
SUPFAMiSSF48056. SSF48056. 1 hit.
PROSITEiPS00497. TYROSINASE_1. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P54834-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLAALCCLL WSFRTSTGHF PRACASSKSL MEKECCPPWS GDGSPCGQLS
60 70 80 90 100
GRGACQDIIL SNAPFGPQFP FTGVDDRESW PSVFYNRTCQ CFGNFMGFNC
110 120 130 140 150
GNCKFGFWGQ NCTEKRLLVR KNIFDLSVPE KNKFLAYLTL AKHTTSPDYV
160 170 180 190 200
IPTGTYGQMN NGSTPMFNDI NIYDLFVWMH YYVSRDTLLG GSEIWKDIDF
210 220 230 240 250
AHEAPGFLPW HRLFLLLWEQ EIQKLTGDEN FTIPYWDWRD AKSCDICTDE
260 270 280 290 300
YMGGRNPANP NLLSPASFFS SWQIVCTRLE EYNSRQALCD GTPEGPLLRN
310 320 330 340 350
PGNHDKARTP RLPSSADVEF CLSLTQYESD SMDKAANFSF RNTLEGFASP
360 370 380 390 400
LTGIADASQS SMHNALHIYM NGTMSQVPGS ANDPIFLLHH AFVDSIFEQW
410 420 430 440 450
LRRHHPLREV YPEANAPIGH NRESYMVPFI PLYRNGDLFI SSRDLGYDYS
460 470 480 490 500
NLQESERDIF QDYIKPYLEQ ASRIWPWLIG AAVVGCVVTA VLGGLTSLLC
510 520 530
RRNRKQLHEE KQPLLMEKED YHSLLYQTHL
Length:530
Mass (Da):60,336
Last modified:April 12, 2005 - v2
Checksum:iB1C45F6362ACF0E3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3L → V in AAA86420 (Ref. 2) Curated1
Sequence conflicti7C → R in AAA86420 (Ref. 2) Curated1
Sequence conflicti59I → V in AAA86420 (Ref. 2) Curated1
Sequence conflicti115K → R in AAA86420 (Ref. 2) Curated1
Sequence conflicti132N → D in AAA86420 (Ref. 2) Curated1
Sequence conflicti212R → T in AAA86420 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY336053 mRNA. Translation: AAQ17535.1.
U42219 Genomic DNA. Translation: AAA86420.1.
RefSeqiNP_001002941.1. NM_001002941.1.
UniGeneiCfa.104.

Genome annotation databases

GeneIDi403405.
KEGGicfa:403405.

Cross-referencesi

Web resourcesi

Protein Spotlight

Snowy stardom - Issue 49 of August 2004

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY336053 mRNA. Translation: AAQ17535.1.
U42219 Genomic DNA. Translation: AAA86420.1.
RefSeqiNP_001002941.1. NM_001002941.1.
UniGeneiCfa.104.

3D structure databases

ProteinModelPortaliP54834.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000006504.

Proteomic databases

PaxDbiP54834.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi403405.
KEGGicfa:403405.

Organism-specific databases

CTDi7299.

Phylogenomic databases

eggNOGiENOG410IEZU. Eukaryota.
ENOG410Y42I. LUCA.
HOGENOMiHOG000118376.
HOVERGENiHBG003553.
InParanoidiP54834.
KOiK00505.

Family and domain databases

Gene3Di1.10.1280.10. 1 hit.
InterProiIPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view]
PfamiPF00264. Tyrosinase. 1 hit.
[Graphical view]
PRINTSiPR00092. TYROSINASE.
SUPFAMiSSF48056. SSF48056. 1 hit.
PROSITEiPS00497. TYROSINASE_1. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTYRO_CANLF
AccessioniPrimary (citable) accession number: P54834
Secondary accession number(s): Q7YRB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: April 12, 2005
Last modified: November 2, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.