Reviewed, UniProtKB/Swiss-Prot P54832 (PON2_CANFA)

Names and origin

Protein names

Recommended name:
    Serum paraoxonase/arylesterase 2
      Short name=PON 2
    EC=3.1.1.2
    EC=3.1.8.1
Alternative name(s):
    Serum aryldialkylphosphatase 2
    Aromatic esterase 2
      Short name=A-esterase 2

Gene names

Name:

PON2

Organism

Canis familiaris (Dog)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and a number of aromatic carboxylic acid esters.
Catalytic activity	A phenyl acetate + H2O = a phenol + acetate. An aryl dialkyl phosphate + H2O = dialkyl phosphate + an aryl alcohol.
Cofactor	Binds 2 calcium ions per subunit By similarity.
Subcellular location	Membrane; Peripheral membrane protein By similarity.
Post-translational modification	Glycosylated By similarity. The signal sequence is not cleaved By similarity.
Sequence similarities	Belongs to the paraoxonase family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: InterPro extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aryldialkylphosphatase activity Inferred from electronic annotation. Source: EC arylesterase activity Inferred from electronic annotation. Source: EC calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Serum paraoxonase/arylesterase 2		PRO_0000223286
	Signal peptide	1 – ?		Not cleaved Potential
Sites
	Active site	114	1	Proton acceptor By similarity
	Metal binding	53	1	Calcium 1; catalytic By similarity
	Metal binding	54	1	Calcium 2 By similarity
	Metal binding	116	1	Calcium 2; via carbonyl oxygen By similarity
	Metal binding	167	1	Calcium 1; catalytic By similarity
	Metal binding	168	1	Calcium 2 By similarity
	Metal binding	223	1	Calcium 1; catalytic By similarity
	Metal binding	268	1	Calcium 1; catalytic By similarity
	Metal binding	269	1	Calcium 1; catalytic By similarity
Amino acid modifications
	Glycosylation	254	1	N-linked (GlcNAc...) Potential
	Glycosylation	269	1	N-linked (GlcNAc...) Potential
	Glycosylation	323	1	N-linked (GlcNAc...) Potential
	Disulfide bond	42 ↔ 352		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P54832-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 2E7A007D800DDCD4	FASTA	354	39,639
	10 20 30 40 50 60 MGRLLGVGLL GDRWRSWGER LLALRNRLKA SREVESVDLP NCHLIKGIEA GADDIDILPN 70 80 90 100 110 120 GLAFFSVGLK CPGLHSFSPD KPGGILMMDL KKENPRALEL RISRGFNLAS FNPHGISTFI 130 140 150 160 170 180 DSDDTVYLFV VNHPEFKNTV EIFKFEEEEN SLLHLKTIKH ELLPSVNDII AVGPAHFYAT 190 200 210 220 230 240 NDHYFSDPFL KYLETYLNLH WANVVYYSPD EVKVVAEGFD AANGINISPD KKYIYVADIL 250 260 270 280 290 300 AHEIHVLEKH PNMNLTQLKV LKLDTLVDNL SIDPSSGDIL VGCHPNGQKL FIYDPNNPPS 310 320 330 340 350 SEVLRIQNIL CEKPTVTTVY ANNGSVLQGS SVASVYDRKL LIGTLYHRAL YCEL

References

[1]	"The human serum paraoxonase/arylesterase gene (PON1) is one member of a multigene family." Primo-Parmo S.L., Sorenson R.C., Teiber J., La Du B.N. Genomics 33:498-507(1996) [PubMed: 8661009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Mongrel. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L48515 mRNA. Translation: AAC41635.1.
RefSeq	NP_001003205.1.
UniGene	Cfa.3715
3D structure databases
SMR	P54832. Positions 23-354.
ModBase	Search...
Protein-protein interaction databases
STRING	P54832.
Genome annotation databases
Ensembl	ENSCAFT00000003349; ENSCAFP00000003109; ENSCAFG00000002123; Canis familiaris. [Genome view]
GeneID	403855.
KEGG	cfa:403855.
Organism-specific databases
CTD	403855.
Phylogenomic databases
eggNOG	maNOG17971.
HOVERGEN	P54832.
InParanoid	P54832.
Enzyme and pathway databases
BRENDA	3.1.1.2. 463. 3.1.8.1. 463.
Family and domain databases
InterPro	IPR011042. 6-blade_b-propeller_TolB-like. IPR002640. Arylesterase. IPR008364. Paraoxonase2. [Graphical view]
Gene3D	G3DSA:2.120.10.30. 6-blade_b-propeller_TolB-like. 1 hit.
Pfam	PF01731. Arylesterase. 1 hit. [Graphical view]
PRINTS	PR01785. PARAOXONASE. PR01787. PARAOXONASE2.
ProtoNet	Search...

Entry information

Entry name

PON2_CANFA

Accession

Primary (citable) accession number: P54832

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	February 9, 2010
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)