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P54830 (PTN5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tyrosine-protein phosphatase non-receptor type 5

EC=3.1.3.48
Alternative name(s):
Neural-specific protein-tyrosine phosphatase
Striatum-enriched protein-tyrosine phosphatase
Short name=STEP
Gene names
Name:Ptpn5
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length541 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May regulate the activity of several effector molecules involved in synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors By similarity.

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Subcellular location

Isoform STEP61: Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.5.

Isoform STEP46: Cytoplasm Ref.5.

Tissue specificity

STEP20 is expressed only in the CNS.

Post-translational modification

Phosphorylation at Ser-221 by PKA deactivates PTPN5. Phosphorylation at Thr-231 and Ser-244 by MAPKs stabilizes the phosphatase, dephosphorylation of these sites results in ubiquitin-mediated degradation of the active phosphatase By similarity.

Sequence similarities

Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.

Contains 1 tyrosine-protein phosphatase domain.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform STEP61 (identifier: P54830-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform STEP46 (identifier: P54830-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-172: Missing.
Isoform STEP38 (identifier: P54830-3)

The sequence of this isoform differs from the canonical sequence as follows:
     337-346: GYSGEEKVYI → VCSSIPRAFH
     347-541: Missing.
Note: Lacks the catalytic domain.
Isoform STEP20 (identifier: P54830-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-172: Missing.
     337-346: GYSGEEKVYI → VCSSIPRAFH
     347-541: Missing.
Note: Lacks the catalytic domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 541541Tyrosine-protein phosphatase non-receptor type 5
PRO_0000094756

Regions

Transmembrane64 – 8421Helical; Potential
Transmembrane122 – 14221Helical; Potential
Domain276 – 531256Tyrosine-protein phosphatase
Region472 – 4787Substrate binding By similarity

Sites

Active site4721Phosphocysteine intermediate By similarity
Binding site4371Substrate By similarity
Binding site5161Substrate By similarity

Amino acid modifications

Modified residue2211Phosphoserine; by PKA By similarity
Modified residue2311Phosphothreonine; by MAPK By similarity
Modified residue2441Phosphoserine; by MAPK By similarity

Natural variations

Alternative sequence1 – 172172Missing in isoform STEP20 and isoform STEP46.
VSP_005126
Alternative sequence337 – 34610GYSGEEKVYI → VCSSIPRAFH in isoform STEP38 and isoform STEP20.
VSP_005127
Alternative sequence347 – 541195Missing in isoform STEP38 and isoform STEP20.
VSP_005128

Experimental info

Sequence conflict4261S → T in AAA73574. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform STEP61 [UniParc].

Last modified February 10, 2009. Version 2.
Checksum: 31A0D2C9139F90A4

FASTA54160,815
        10         20         30         40         50         60 
MCCSERLLGL PQPVEMEAPD EAEGLPSKQK EMPPPPPPSP PSEPAQKLPP QGAGSHSLTV 

        70         80         90        100        110        120 
RSSLCLFAAS QFLLACGVLW LSGHGHSWLQ NTTDLISSSL TVLNHLGPVA WLGSGTWGIP 

       130        140        150        160        170        180 
SLLLVSLTVS LVIVTTLVWH LLKAPPEPPA PLPPEDRRQS VSRQPSFTYS EWMEEKVEDD 

       190        200        210        220        230        240 
FLDLDAVPET PVFDCVMDIK PETDPASLTV KSMGLQERRG SNVSLTLDMC TPGCNEEGFG 

       250        260        270        280        290        300 
YLVSPREESA HEYLLSASRV LRAEELHEKA LDPFLLQAEF FEIPMNFVDP KEYDIPGLVR 

       310        320        330        340        350        360 
KNRYKTILPN PHSRVRLTSP DPEDPLSSYI NANYIRGYSG EEKVYIATQG PIVSTVADFW 

       370        380        390        400        410        420 
RMVWQERTPI IVMITNIEEM NEKCTEYWPE EQVVHDGVEI TVQKVIHTED YRLRLISLRR 

       430        440        450        460        470        480 
GTEERSLKHY WFTSWPDQKT PDRAPPLLHL VREVEEAAQQ EGPHCSPIIV HCSAGIGRTG 

       490        500        510        520        530        540 
CFIATSICCQ QLRREGVVDI LKTTCQLRQD RGGMIQTCEQ YQFVHHAMSL YEKQLSLQSS 


E 

« Hide

Isoform STEP46 [UniParc].

Checksum: 562A8FA130A7D186
Show »

FASTA36942,341
Isoform STEP38 [UniParc].

Checksum: E40E188D64C2263B
Show »

FASTA34638,238
Isoform STEP20 [UniParc].

Checksum: 19CDAB71DA14EAA0
Show »

FASTA17419,763

References

« Hide 'large scale' references
[1]"Identification of two alternatively spliced transcripts of STEP: a subfamily of brain-enriched protein tyrosine phosphatases."
Sharma E., Zhao F., Bult A., Lombroso P.J.
Brain Res. Mol. Brain Res. 32:87-93(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS STEP20; STEP38; STEP46 AND STEP61).
Strain: BALB/c.
Tissue: Brain.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM STEP61).
Strain: C57BL/6J.
Tissue: Hypothalamus.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM STEP61).
Strain: C57BL/6.
Tissue: Brain.
[5]"STEP61: a member of a family of brain-enriched PTPs is localized to the endoplasmic reticulum."
Bult A., Zhao F., Dirkx R. Jr., Sharma E., Lukacsi E., Solimena M., Naegele J.R., Lombroso P.J.
J. Neurosci. 16:7821-7831(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U28217 mRNA. Translation: AAA73574.1.
U28216 mRNA. Translation: AAA73573.1.
S80329 mRNA. Translation: AAB35656.2.
AK038416 mRNA. Translation: BAC29993.1.
CH466603 Genomic DNA. Translation: EDL22958.1.
BC079592 mRNA. Translation: AAH79592.1.
CCDSCCDS21294.1. [P54830-1]
RefSeqNP_001157037.1. NM_001163565.1. [P54830-1]
NP_038671.2. NM_013643.2. [P54830-1]
XP_006540776.1. XM_006540713.1. [P54830-1]
XP_006540777.1. XM_006540714.1. [P54830-1]
XP_006540778.1. XM_006540715.1. [P54830-1]
XP_006540779.1. XM_006540716.1. [P54830-1]
UniGeneMm.4654.

3D structure databases

ProteinModelPortalP54830.
SMRP54830. Positions 256-536.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid202488. 1 interaction.
DIPDIP-32453N.
IntActP54830. 3 interactions.
MINTMINT-4109079.

PTM databases

PhosphoSiteP54830.

Proteomic databases

PaxDbP54830.
PRIDEP54830.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000033142; ENSMUSP00000033142; ENSMUSG00000030854. [P54830-1]
ENSMUST00000102626; ENSMUSP00000099686; ENSMUSG00000030854. [P54830-1]
ENSMUST00000107617; ENSMUSP00000103242; ENSMUSG00000030854. [P54830-3]
GeneID19259.
KEGGmmu:19259.
UCSCuc009gzz.2. mouse. [P54830-1]
uc009hac.1. mouse. [P54830-3]

Organism-specific databases

CTD84867.
MGIMGI:97807. Ptpn5.

Phylogenomic databases

eggNOGCOG5599.
GeneTreeENSGT00750000117606.
HOVERGENHBG001594.
InParanoidQ8CAN0.
KOK18018.
OMACTPGCSE.
OrthoDBEOG7288QX.
TreeFamTF331016.

Gene expression databases

ArrayExpressP54830.
BgeeP54830.
CleanExMM_PTPN5.
GenevestigatorP54830.

Family and domain databases

Gene3D3.90.190.10. 1 hit.
InterProIPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR008356. Tyr_Pase_KIM-con.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
IPR016334. Tyr_Pase_rcpt_R/non-rcpt_5.
[Graphical view]
PfamPF00102. Y_phosphatase. 1 hit.
[Graphical view]
PIRSFPIRSF001997. PTPRR. 1 hit.
PRINTSPR01778. KIMPTPASE.
PR00700. PRTYPHPHTASE.
SMARTSM00194. PTPc. 1 hit.
[Graphical view]
SUPFAMSSF52799. SSF52799. 1 hit.
PROSITEPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio296112.
PROP54830.
SOURCESearch...

Entry information

Entry namePTN5_MOUSE
AccessionPrimary (citable) accession number: P54830
Secondary accession number(s): Q64694, Q8CAN0
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: February 10, 2009
Last modified: July 9, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot