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P54830

- PTN5_MOUSE

UniProt

P54830 - PTN5_MOUSE

Protein

Tyrosine-protein phosphatase non-receptor type 5

Gene

Ptpn5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 2 (10 Feb 2009)
      Previous versions | rss
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    Functioni

    May regulate the activity of several effector molecules involved in synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors.By similarity

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei437 – 4371SubstrateBy similarity
    Active sitei472 – 4721Phosphocysteine intermediatePROSITE-ProRule annotation
    Binding sitei516 – 5161SubstrateBy similarity

    GO - Molecular functioni

    1. protein tyrosine phosphatase activity Source: MGI

    GO - Biological processi

    1. peptidyl-tyrosine dephosphorylation Source: GOC
    2. protein dephosphorylation Source: MGI

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tyrosine-protein phosphatase non-receptor type 5 (EC:3.1.3.48)
    Alternative name(s):
    Neural-specific protein-tyrosine phosphatase
    Striatum-enriched protein-tyrosine phosphatase
    Short name:
    STEP
    Gene namesi
    Name:Ptpn5
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:97807. Ptpn5.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasm, Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 541541Tyrosine-protein phosphatase non-receptor type 5PRO_0000094756Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei221 – 2211Phosphoserine; by PKABy similarity
    Modified residuei231 – 2311Phosphothreonine; by MAPKBy similarity
    Modified residuei244 – 2441Phosphoserine; by MAPKBy similarity

    Post-translational modificationi

    Phosphorylation at Ser-221 by PKA deactivates PTPN5. Phosphorylation at Thr-231 and Ser-244 by MAPKs stabilizes the phosphatase, dephosphorylation of these sites results in ubiquitin-mediated degradation of the active phosphatase By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP54830.
    PRIDEiP54830.

    PTM databases

    PhosphoSiteiP54830.

    Expressioni

    Tissue specificityi

    STEP20 is expressed only in the CNS.

    Gene expression databases

    ArrayExpressiP54830.
    BgeeiP54830.
    CleanExiMM_PTPN5.
    GenevestigatoriP54830.

    Interactioni

    Protein-protein interaction databases

    BioGridi202488. 1 interaction.
    DIPiDIP-32453N.
    IntActiP54830. 3 interactions.
    MINTiMINT-4109079.

    Structurei

    3D structure databases

    ProteinModelPortaliP54830.
    SMRiP54830. Positions 256-536.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei64 – 8421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei122 – 14221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini276 – 531256Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni472 – 4787Substrate bindingBy similarity

    Sequence similaritiesi

    Contains 1 tyrosine-protein phosphatase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5599.
    GeneTreeiENSGT00750000117606.
    HOVERGENiHBG001594.
    InParanoidiQ8CAN0.
    KOiK18018.
    OMAiCTPGCSE.
    OrthoDBiEOG7288QX.
    TreeFamiTF331016.

    Family and domain databases

    Gene3Di3.90.190.10. 1 hit.
    InterProiIPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    IPR008356. Tyr_Pase_KIM-con.
    IPR000242. Tyr_Pase_rcpt/non-rcpt.
    IPR016334. Tyr_Pase_rcpt_R/non-rcpt_5.
    [Graphical view]
    PfamiPF00102. Y_phosphatase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001997. PTPRR. 1 hit.
    PRINTSiPR01778. KIMPTPASE.
    PR00700. PRTYPHPHTASE.
    SMARTiSM00194. PTPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 1 hit.
    PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Note: Additional isoforms seem to exist.

    Isoform STEP61 (identifier: P54830-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MCCSERLLGL PQPVEMEAPD EAEGLPSKQK EMPPPPPPSP PSEPAQKLPP    50
    QGAGSHSLTV RSSLCLFAAS QFLLACGVLW LSGHGHSWLQ NTTDLISSSL 100
    TVLNHLGPVA WLGSGTWGIP SLLLVSLTVS LVIVTTLVWH LLKAPPEPPA 150
    PLPPEDRRQS VSRQPSFTYS EWMEEKVEDD FLDLDAVPET PVFDCVMDIK 200
    PETDPASLTV KSMGLQERRG SNVSLTLDMC TPGCNEEGFG YLVSPREESA 250
    HEYLLSASRV LRAEELHEKA LDPFLLQAEF FEIPMNFVDP KEYDIPGLVR 300
    KNRYKTILPN PHSRVRLTSP DPEDPLSSYI NANYIRGYSG EEKVYIATQG 350
    PIVSTVADFW RMVWQERTPI IVMITNIEEM NEKCTEYWPE EQVVHDGVEI 400
    TVQKVIHTED YRLRLISLRR GTEERSLKHY WFTSWPDQKT PDRAPPLLHL 450
    VREVEEAAQQ EGPHCSPIIV HCSAGIGRTG CFIATSICCQ QLRREGVVDI 500
    LKTTCQLRQD RGGMIQTCEQ YQFVHHAMSL YEKQLSLQSS E 541
    Length:541
    Mass (Da):60,815
    Last modified:February 10, 2009 - v2
    Checksum:i31A0D2C9139F90A4
    GO
    Isoform STEP46 (identifier: P54830-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-172: Missing.

    Show »
    Length:369
    Mass (Da):42,341
    Checksum:i562A8FA130A7D186
    GO
    Isoform STEP38 (identifier: P54830-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         337-346: GYSGEEKVYI → VCSSIPRAFH
         347-541: Missing.

    Note: Lacks the catalytic domain.

    Show »
    Length:346
    Mass (Da):38,238
    Checksum:iE40E188D64C2263B
    GO
    Isoform STEP20 (identifier: P54830-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-172: Missing.
         337-346: GYSGEEKVYI → VCSSIPRAFH
         347-541: Missing.

    Note: Lacks the catalytic domain.

    Show »
    Length:174
    Mass (Da):19,763
    Checksum:i19CDAB71DA14EAA0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti426 – 4261S → T in AAA73574. (PubMed:7494467)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 172172Missing in isoform STEP20 and isoform STEP46. 1 PublicationVSP_005126Add
    BLAST
    Alternative sequencei337 – 34610GYSGEEKVYI → VCSSIPRAFH in isoform STEP38 and isoform STEP20. 1 PublicationVSP_005127
    Alternative sequencei347 – 541195Missing in isoform STEP38 and isoform STEP20. 1 PublicationVSP_005128Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U28217 mRNA. Translation: AAA73574.1.
    U28216 mRNA. Translation: AAA73573.1.
    S80329 mRNA. Translation: AAB35656.2.
    AK038416 mRNA. Translation: BAC29993.1.
    CH466603 Genomic DNA. Translation: EDL22958.1.
    BC079592 mRNA. Translation: AAH79592.1.
    CCDSiCCDS21294.1. [P54830-1]
    RefSeqiNP_001157037.1. NM_001163565.1. [P54830-1]
    NP_038671.2. NM_013643.2. [P54830-1]
    XP_006540776.1. XM_006540713.1. [P54830-1]
    XP_006540777.1. XM_006540714.1. [P54830-1]
    XP_006540778.1. XM_006540715.1. [P54830-1]
    XP_006540779.1. XM_006540716.1. [P54830-1]
    UniGeneiMm.4654.

    Genome annotation databases

    EnsembliENSMUST00000033142; ENSMUSP00000033142; ENSMUSG00000030854. [P54830-1]
    ENSMUST00000102626; ENSMUSP00000099686; ENSMUSG00000030854. [P54830-1]
    ENSMUST00000107617; ENSMUSP00000103242; ENSMUSG00000030854. [P54830-3]
    GeneIDi19259.
    KEGGimmu:19259.
    UCSCiuc009gzz.2. mouse. [P54830-1]
    uc009hac.1. mouse. [P54830-3]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U28217 mRNA. Translation: AAA73574.1 .
    U28216 mRNA. Translation: AAA73573.1 .
    S80329 mRNA. Translation: AAB35656.2 .
    AK038416 mRNA. Translation: BAC29993.1 .
    CH466603 Genomic DNA. Translation: EDL22958.1 .
    BC079592 mRNA. Translation: AAH79592.1 .
    CCDSi CCDS21294.1. [P54830-1 ]
    RefSeqi NP_001157037.1. NM_001163565.1. [P54830-1 ]
    NP_038671.2. NM_013643.2. [P54830-1 ]
    XP_006540776.1. XM_006540713.1. [P54830-1 ]
    XP_006540777.1. XM_006540714.1. [P54830-1 ]
    XP_006540778.1. XM_006540715.1. [P54830-1 ]
    XP_006540779.1. XM_006540716.1. [P54830-1 ]
    UniGenei Mm.4654.

    3D structure databases

    ProteinModelPortali P54830.
    SMRi P54830. Positions 256-536.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202488. 1 interaction.
    DIPi DIP-32453N.
    IntActi P54830. 3 interactions.
    MINTi MINT-4109079.

    PTM databases

    PhosphoSitei P54830.

    Proteomic databases

    PaxDbi P54830.
    PRIDEi P54830.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000033142 ; ENSMUSP00000033142 ; ENSMUSG00000030854 . [P54830-1 ]
    ENSMUST00000102626 ; ENSMUSP00000099686 ; ENSMUSG00000030854 . [P54830-1 ]
    ENSMUST00000107617 ; ENSMUSP00000103242 ; ENSMUSG00000030854 . [P54830-3 ]
    GeneIDi 19259.
    KEGGi mmu:19259.
    UCSCi uc009gzz.2. mouse. [P54830-1 ]
    uc009hac.1. mouse. [P54830-3 ]

    Organism-specific databases

    CTDi 84867.
    MGIi MGI:97807. Ptpn5.

    Phylogenomic databases

    eggNOGi COG5599.
    GeneTreei ENSGT00750000117606.
    HOVERGENi HBG001594.
    InParanoidi Q8CAN0.
    KOi K18018.
    OMAi CTPGCSE.
    OrthoDBi EOG7288QX.
    TreeFami TF331016.

    Miscellaneous databases

    NextBioi 296112.
    PROi P54830.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P54830.
    Bgeei P54830.
    CleanExi MM_PTPN5.
    Genevestigatori P54830.

    Family and domain databases

    Gene3Di 3.90.190.10. 1 hit.
    InterProi IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    IPR008356. Tyr_Pase_KIM-con.
    IPR000242. Tyr_Pase_rcpt/non-rcpt.
    IPR016334. Tyr_Pase_rcpt_R/non-rcpt_5.
    [Graphical view ]
    Pfami PF00102. Y_phosphatase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001997. PTPRR. 1 hit.
    PRINTSi PR01778. KIMPTPASE.
    PR00700. PRTYPHPHTASE.
    SMARTi SM00194. PTPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52799. SSF52799. 1 hit.
    PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of two alternatively spliced transcripts of STEP: a subfamily of brain-enriched protein tyrosine phosphatases."
      Sharma E., Zhao F., Bult A., Lombroso P.J.
      Brain Res. Mol. Brain Res. 32:87-93(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS STEP20; STEP38; STEP46 AND STEP61).
      Strain: BALB/c.
      Tissue: Brain.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM STEP61).
      Strain: C57BL/6J.
      Tissue: Hypothalamus.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM STEP61).
      Strain: C57BL/6.
      Tissue: Brain.
    5. "STEP61: a member of a family of brain-enriched PTPs is localized to the endoplasmic reticulum."
      Bult A., Zhao F., Dirkx R. Jr., Sharma E., Lukacsi E., Solimena M., Naegele J.R., Lombroso P.J.
      J. Neurosci. 16:7821-7831(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiPTN5_MOUSE
    AccessioniPrimary (citable) accession number: P54830
    Secondary accession number(s): Q64694, Q8CAN0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: February 10, 2009
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3