P54830 (PTN5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein phosphatase non-receptor type 5
EC=3.1.3.48

Alternative name(s):
Neural-specific protein-tyrosine phosphatase
Striatum-enriched protein-tyrosine phosphatase
Short name=STEP

Gene names

Name:

Ptpn5

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	541 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May regulate the activity of several effector molecules involved in synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors By similarity.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Subcellular location	Isoform STEP61: Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.5. Isoform STEP46: Cytoplasm Ref.5.
Tissue specificity	STEP20 is expressed only in the CNS.
Post-translational modification	Phosphorylation at Ser-221 by PKA deactivates PTPN5. Phosphorylation at Thr-231 and Ser-244 by MAPKs stabilizes the phosphatase, dephosphorylation of these sites results in ubiquitin-mediated degradation of the active phosphatase By similarity.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Endoplasmic reticulum Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase Protein phosphatase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	protein tyrosine phosphatase activity Inferred from mutant phenotype PubMed 16025111. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform STEP61 (identifier: P54830-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform STEP46 (identifier: P54830-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-172: Missing.

Isoform STEP38 (identifier: P54830-3)

The sequence of this isoform differs from the canonical sequence as follows:
     337-346: GYSGEEKVYI → VCSSIPRAFH
     347-541: Missing.

Note: Lacks the catalytic domain.

Isoform STEP20 (identifier: P54830-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-172: Missing.
     337-346: GYSGEEKVYI → VCSSIPRAFH
     347-541: Missing.

Note: Lacks the catalytic domain.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 541	541	Tyrosine-protein phosphatase non-receptor type 5		PRO_0000094756
Regions
	Transmembrane	64 – 84	21	Helical; Potential
	Transmembrane	122 – 142	21	Helical; Potential
	Domain	276 – 531	256	Tyrosine-protein phosphatase
	Region	472 – 478	7	Substrate binding By similarity
Sites
	Active site	472	1	Phosphocysteine intermediate By similarity
	Binding site	437	1	Substrate By similarity
	Binding site	516	1	Substrate By similarity
Amino acid modifications
	Modified residue	221	1	Phosphoserine; by PKA By similarity
	Modified residue	231	1	Phosphothreonine; by MAPK By similarity
	Modified residue	244	1	Phosphoserine; by MAPK By similarity
Natural variations
	Alternative sequence	1 – 172	172	Missing in isoform STEP20 and isoform STEP46.		VSP_005126
	Alternative sequence	337 – 346	10	GYSGEEKVYI → VCSSIPRAFH in isoform STEP38 and isoform STEP20.		VSP_005127
	Alternative sequence	347 – 541	195	Missing in isoform STEP38 and isoform STEP20.		VSP_005128
Experimental info
	Sequence conflict	426	1	S → T in AAA73574. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform STEP61 [UniParc]. Last modified February 10, 2009. Version 2. Checksum: 31A0D2C9139F90A4	FASTA	541	60,815
	10 20 30 40 50 60 MCCSERLLGL PQPVEMEAPD EAEGLPSKQK EMPPPPPPSP PSEPAQKLPP QGAGSHSLTV 70 80 90 100 110 120 RSSLCLFAAS QFLLACGVLW LSGHGHSWLQ NTTDLISSSL TVLNHLGPVA WLGSGTWGIP 130 140 150 160 170 180 SLLLVSLTVS LVIVTTLVWH LLKAPPEPPA PLPPEDRRQS VSRQPSFTYS EWMEEKVEDD 190 200 210 220 230 240 FLDLDAVPET PVFDCVMDIK PETDPASLTV KSMGLQERRG SNVSLTLDMC TPGCNEEGFG 250 260 270 280 290 300 YLVSPREESA HEYLLSASRV LRAEELHEKA LDPFLLQAEF FEIPMNFVDP KEYDIPGLVR 310 320 330 340 350 360 KNRYKTILPN PHSRVRLTSP DPEDPLSSYI NANYIRGYSG EEKVYIATQG PIVSTVADFW 370 380 390 400 410 420 RMVWQERTPI IVMITNIEEM NEKCTEYWPE EQVVHDGVEI TVQKVIHTED YRLRLISLRR 430 440 450 460 470 480 GTEERSLKHY WFTSWPDQKT PDRAPPLLHL VREVEEAAQQ EGPHCSPIIV HCSAGIGRTG 490 500 510 520 530 540 CFIATSICCQ QLRREGVVDI LKTTCQLRQD RGGMIQTCEQ YQFVHHAMSL YEKQLSLQSS E
	Isoform STEP46 [UniParc]. Checksum: 562A8FA130A7D186	FASTA	369	42,341
	10 20 30 40 50 60 MEEKVEDDFL DLDAVPETPV FDCVMDIKPE TDPASLTVKS MGLQERRGSN VSLTLDMCTP 70 80 90 100 110 120 GCNEEGFGYL VSPREESAHE YLLSASRVLR AEELHEKALD PFLLQAEFFE IPMNFVDPKE 130 140 150 160 170 180 YDIPGLVRKN RYKTILPNPH SRVRLTSPDP EDPLSSYINA NYIRGYSGEE KVYIATQGPI 190 200 210 220 230 240 VSTVADFWRM VWQERTPIIV MITNIEEMNE KCTEYWPEEQ VVHDGVEITV QKVIHTEDYR 250 260 270 280 290 300 LRLISLRRGT EERSLKHYWF TSWPDQKTPD RAPPLLHLVR EVEEAAQQEG PHCSPIIVHC 310 320 330 340 350 360 SAGIGRTGCF IATSICCQQL RREGVVDILK TTCQLRQDRG GMIQTCEQYQ FVHHAMSLYE KQLSLQSSE
	Isoform STEP38 [UniParc]. Checksum: E40E188D64C2263B	FASTA	346	38,238
	10 20 30 40 50 60 MCCSERLLGL PQPVEMEAPD EAEGLPSKQK EMPPPPPPSP PSEPAQKLPP QGAGSHSLTV 70 80 90 100 110 120 RSSLCLFAAS QFLLACGVLW LSGHGHSWLQ NTTDLISSSL TVLNHLGPVA WLGSGTWGIP 130 140 150 160 170 180 SLLLVSLTVS LVIVTTLVWH LLKAPPEPPA PLPPEDRRQS VSRQPSFTYS EWMEEKVEDD 190 200 210 220 230 240 FLDLDAVPET PVFDCVMDIK PETDPASLTV KSMGLQERRG SNVSLTLDMC TPGCNEEGFG 250 260 270 280 290 300 YLVSPREESA HEYLLSASRV LRAEELHEKA LDPFLLQAEF FEIPMNFVDP KEYDIPGLVR 310 320 330 340 KNRYKTILPN PHSRVRLTSP DPEDPLSSYI NANYIRVCSS IPRAFH
	Isoform STEP20 [UniParc]. Checksum: 19CDAB71DA14EAA0	FASTA	174	19,763
	10 20 30 40 50 60 MEEKVEDDFL DLDAVPETPV FDCVMDIKPE TDPASLTVKS MGLQERRGSN VSLTLDMCTP 70 80 90 100 110 120 GCNEEGFGYL VSPREESAHE YLLSASRVLR AEELHEKALD PFLLQAEFFE IPMNFVDPKE 130 140 150 160 170 YDIPGLVRKN RYKTILPNPH SRVRLTSPDP EDPLSSYINA NYIRVCSSIP RAFH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of two alternatively spliced transcripts of STEP: a subfamily of brain-enriched protein tyrosine phosphatases." Sharma E., Zhao F., Bult A., Lombroso P.J. Brain Res. Mol. Brain Res. 32:87-93(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS STEP20; STEP38; STEP46 AND STEP61). Strain: BALB/c. Tissue: Brain.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM STEP61). Strain: C57BL/6J. Tissue: Hypothalamus.
[3]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM STEP61). Strain: C57BL/6. Tissue: Brain.
[5]	"STEP61: a member of a family of brain-enriched PTPs is localized to the endoplasmic reticulum." Bult A., Zhao F., Dirkx R. Jr., Sharma E., Lukacsi E., Solimena M., Naegele J.R., Lombroso P.J. J. Neurosci. 16:7821-7831(1996) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U28217 mRNA. Translation: AAA73574.1. U28216 mRNA. Translation: AAA73573.1. S80329 mRNA. Translation: AAB35656.2. AK038416 mRNA. Translation: BAC29993.1. CH466603 Genomic DNA. Translation: EDL22958.1. BC079592 mRNA. Translation: AAH79592.1.
IPI	IPI00275198. IPI00331399. IPI00467661. IPI00474037.
RefSeq	NP_001157037.1. NM_001163565.1. NP_038671.2. NM_013643.2.
UniGene	Mm.4654.
3D structure databases
ProteinModelPortal	P54830.
SMR	P54830. Positions 256-536.
ModBase	Search...
Protein-protein interaction databases
IntAct	P54830. 2 interactions.
MINT	MINT-4109079.
PTM databases
PhosphoSite	P54830.
Proteomic databases
PaxDb	P54830.
PRIDE	P54830.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000033142; ENSMUSP00000033142; ENSMUSG00000030854. ENSMUST00000102626; ENSMUSP00000099686; ENSMUSG00000030854. ENSMUST00000107617; ENSMUSP00000103242; ENSMUSG00000030854.
GeneID	19259.
KEGG	mmu:19259.
UCSC	uc009gzz.2. mouse. uc009hac.1. mouse.
Organism-specific databases
CTD	84867.
MGI	MGI:97807. Ptpn5.
Phylogenomic databases
eggNOG	COG5599.
GeneTree	ENSGT00700000104095.
HOVERGEN	HBG001594.
InParanoid	Q8CAN0.
KO	K04458.
OMA	CTPGCSE.
OrthoDB	EOG4229JX.
Gene expression databases
ArrayExpress	P54830.
Bgee	P54830.
CleanEx	MM_PTPN5.
Genevestigator	P54830.
GermOnline	ENSMUSG00000030854. Mus musculus.
Family and domain databases
InterPro	IPR000387. Tyr/Dual-sp_Pase. IPR016130. Tyr_Pase_AS. IPR008356. Tyr_Pase_KIM-con. IPR000242. Tyr_Pase_rcpt/non-rcpt. IPR016334. Tyr_Pase_rcpt_R/non-rcpt_5. [Graphical view]
Pfam	PF00102. Y_phosphatase. 1 hit. [Graphical view]
PIRSF	PIRSF001997. PTPRR. 1 hit.
PRINTS	PR01778. KIMPTPASE. PR00700. PRTYPHPHTASE.
SMART	SM00194. PTPc. 1 hit. [Graphical view]
PROSITE	PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50055. TYR_PHOSPHATASE_PTP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	296112.
SOURCE	Search...

Entry information

Entry name

PTN5_MOUSE

Accession

Primary (citable) accession number: P54830
Secondary accession number(s): Q64694, Q8CAN0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	February 10, 2009
Last modified:	May 29, 2013
This is version 106 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program