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P54830

- PTN5_MOUSE

UniProt

P54830 - PTN5_MOUSE

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Protein
Tyrosine-protein phosphatase non-receptor type 5
Gene
Ptpn5
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

May regulate the activity of several effector molecules involved in synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors By similarity.

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei437 – 4371Substrate By similarity
Active sitei472 – 4721Phosphocysteine intermediate By similarity
Binding sitei516 – 5161Substrate By similarity

GO - Molecular functioni

  1. protein tyrosine phosphatase activity Source: MGI
Complete GO annotation...

GO - Biological processi

  1. peptidyl-tyrosine dephosphorylation Source: GOC
  2. protein dephosphorylation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein phosphatase non-receptor type 5 (EC:3.1.3.48)
Alternative name(s):
Neural-specific protein-tyrosine phosphatase
Striatum-enriched protein-tyrosine phosphatase
Short name:
STEP
Gene namesi
Name:Ptpn5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:97807. Ptpn5.

Subcellular locationi

Isoform STEP61 : Endoplasmic reticulum membrane; Multi-pass membrane protein 1 Publication
Isoform STEP46 : Cytoplasm 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei64 – 8421Helical; Reviewed prediction
Add
BLAST
Transmembranei122 – 14221Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 541541Tyrosine-protein phosphatase non-receptor type 5
PRO_0000094756Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei221 – 2211Phosphoserine; by PKA By similarity
Modified residuei231 – 2311Phosphothreonine; by MAPK By similarity
Modified residuei244 – 2441Phosphoserine; by MAPK By similarity

Post-translational modificationi

Phosphorylation at Ser-221 by PKA deactivates PTPN5. Phosphorylation at Thr-231 and Ser-244 by MAPKs stabilizes the phosphatase, dephosphorylation of these sites results in ubiquitin-mediated degradation of the active phosphatase By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP54830.
PRIDEiP54830.

PTM databases

PhosphoSiteiP54830.

Expressioni

Tissue specificityi

STEP20 is expressed only in the CNS.

Gene expression databases

ArrayExpressiP54830.
BgeeiP54830.
CleanExiMM_PTPN5.
GenevestigatoriP54830.

Interactioni

Protein-protein interaction databases

BioGridi202488. 1 interaction.
DIPiDIP-32453N.
IntActiP54830. 3 interactions.
MINTiMINT-4109079.

Structurei

3D structure databases

ProteinModelPortaliP54830.
SMRiP54830. Positions 256-536.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini276 – 531256Tyrosine-protein phosphatase
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni472 – 4787Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5599.
GeneTreeiENSGT00750000117606.
HOVERGENiHBG001594.
InParanoidiQ8CAN0.
KOiK18018.
OMAiCTPGCSE.
OrthoDBiEOG7288QX.
TreeFamiTF331016.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR008356. Tyr_Pase_KIM-con.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
IPR016334. Tyr_Pase_rcpt_R/non-rcpt_5.
[Graphical view]
PfamiPF00102. Y_phosphatase. 1 hit.
[Graphical view]
PIRSFiPIRSF001997. PTPRR. 1 hit.
PRINTSiPR01778. KIMPTPASE.
PR00700. PRTYPHPHTASE.
SMARTiSM00194. PTPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Note: Additional isoforms seem to exist.

Isoform STEP61 (identifier: P54830-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MCCSERLLGL PQPVEMEAPD EAEGLPSKQK EMPPPPPPSP PSEPAQKLPP    50
QGAGSHSLTV RSSLCLFAAS QFLLACGVLW LSGHGHSWLQ NTTDLISSSL 100
TVLNHLGPVA WLGSGTWGIP SLLLVSLTVS LVIVTTLVWH LLKAPPEPPA 150
PLPPEDRRQS VSRQPSFTYS EWMEEKVEDD FLDLDAVPET PVFDCVMDIK 200
PETDPASLTV KSMGLQERRG SNVSLTLDMC TPGCNEEGFG YLVSPREESA 250
HEYLLSASRV LRAEELHEKA LDPFLLQAEF FEIPMNFVDP KEYDIPGLVR 300
KNRYKTILPN PHSRVRLTSP DPEDPLSSYI NANYIRGYSG EEKVYIATQG 350
PIVSTVADFW RMVWQERTPI IVMITNIEEM NEKCTEYWPE EQVVHDGVEI 400
TVQKVIHTED YRLRLISLRR GTEERSLKHY WFTSWPDQKT PDRAPPLLHL 450
VREVEEAAQQ EGPHCSPIIV HCSAGIGRTG CFIATSICCQ QLRREGVVDI 500
LKTTCQLRQD RGGMIQTCEQ YQFVHHAMSL YEKQLSLQSS E 541
Length:541
Mass (Da):60,815
Last modified:February 10, 2009 - v2
Checksum:i31A0D2C9139F90A4
GO
Isoform STEP46 (identifier: P54830-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-172: Missing.

Show »
Length:369
Mass (Da):42,341
Checksum:i562A8FA130A7D186
GO
Isoform STEP38 (identifier: P54830-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     337-346: GYSGEEKVYI → VCSSIPRAFH
     347-541: Missing.

Note: Lacks the catalytic domain.

Show »
Length:346
Mass (Da):38,238
Checksum:iE40E188D64C2263B
GO
Isoform STEP20 (identifier: P54830-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-172: Missing.
     337-346: GYSGEEKVYI → VCSSIPRAFH
     347-541: Missing.

Note: Lacks the catalytic domain.

Show »
Length:174
Mass (Da):19,763
Checksum:i19CDAB71DA14EAA0
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 172172Missing in isoform STEP20 and isoform STEP46.
VSP_005126Add
BLAST
Alternative sequencei337 – 34610GYSGEEKVYI → VCSSIPRAFH in isoform STEP38 and isoform STEP20.
VSP_005127
Alternative sequencei347 – 541195Missing in isoform STEP38 and isoform STEP20.
VSP_005128Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti426 – 4261S → T in AAA73574. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U28217 mRNA. Translation: AAA73574.1.
U28216 mRNA. Translation: AAA73573.1.
S80329 mRNA. Translation: AAB35656.2.
AK038416 mRNA. Translation: BAC29993.1.
CH466603 Genomic DNA. Translation: EDL22958.1.
BC079592 mRNA. Translation: AAH79592.1.
CCDSiCCDS21294.1. [P54830-1]
RefSeqiNP_001157037.1. NM_001163565.1. [P54830-1]
NP_038671.2. NM_013643.2. [P54830-1]
XP_006540776.1. XM_006540713.1. [P54830-1]
XP_006540777.1. XM_006540714.1. [P54830-1]
XP_006540778.1. XM_006540715.1. [P54830-1]
XP_006540779.1. XM_006540716.1. [P54830-1]
UniGeneiMm.4654.

Genome annotation databases

EnsembliENSMUST00000033142; ENSMUSP00000033142; ENSMUSG00000030854. [P54830-1]
ENSMUST00000102626; ENSMUSP00000099686; ENSMUSG00000030854. [P54830-1]
ENSMUST00000107617; ENSMUSP00000103242; ENSMUSG00000030854. [P54830-3]
GeneIDi19259.
KEGGimmu:19259.
UCSCiuc009gzz.2. mouse. [P54830-1]
uc009hac.1. mouse. [P54830-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U28217 mRNA. Translation: AAA73574.1 .
U28216 mRNA. Translation: AAA73573.1 .
S80329 mRNA. Translation: AAB35656.2 .
AK038416 mRNA. Translation: BAC29993.1 .
CH466603 Genomic DNA. Translation: EDL22958.1 .
BC079592 mRNA. Translation: AAH79592.1 .
CCDSi CCDS21294.1. [P54830-1 ]
RefSeqi NP_001157037.1. NM_001163565.1. [P54830-1 ]
NP_038671.2. NM_013643.2. [P54830-1 ]
XP_006540776.1. XM_006540713.1. [P54830-1 ]
XP_006540777.1. XM_006540714.1. [P54830-1 ]
XP_006540778.1. XM_006540715.1. [P54830-1 ]
XP_006540779.1. XM_006540716.1. [P54830-1 ]
UniGenei Mm.4654.

3D structure databases

ProteinModelPortali P54830.
SMRi P54830. Positions 256-536.
ModBasei Search...

Protein-protein interaction databases

BioGridi 202488. 1 interaction.
DIPi DIP-32453N.
IntActi P54830. 3 interactions.
MINTi MINT-4109079.

PTM databases

PhosphoSitei P54830.

Proteomic databases

PaxDbi P54830.
PRIDEi P54830.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000033142 ; ENSMUSP00000033142 ; ENSMUSG00000030854 . [P54830-1 ]
ENSMUST00000102626 ; ENSMUSP00000099686 ; ENSMUSG00000030854 . [P54830-1 ]
ENSMUST00000107617 ; ENSMUSP00000103242 ; ENSMUSG00000030854 . [P54830-3 ]
GeneIDi 19259.
KEGGi mmu:19259.
UCSCi uc009gzz.2. mouse. [P54830-1 ]
uc009hac.1. mouse. [P54830-3 ]

Organism-specific databases

CTDi 84867.
MGIi MGI:97807. Ptpn5.

Phylogenomic databases

eggNOGi COG5599.
GeneTreei ENSGT00750000117606.
HOVERGENi HBG001594.
InParanoidi Q8CAN0.
KOi K18018.
OMAi CTPGCSE.
OrthoDBi EOG7288QX.
TreeFami TF331016.

Miscellaneous databases

NextBioi 296112.
PROi P54830.
SOURCEi Search...

Gene expression databases

ArrayExpressi P54830.
Bgeei P54830.
CleanExi MM_PTPN5.
Genevestigatori P54830.

Family and domain databases

Gene3Di 3.90.190.10. 1 hit.
InterProi IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR008356. Tyr_Pase_KIM-con.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
IPR016334. Tyr_Pase_rcpt_R/non-rcpt_5.
[Graphical view ]
Pfami PF00102. Y_phosphatase. 1 hit.
[Graphical view ]
PIRSFi PIRSF001997. PTPRR. 1 hit.
PRINTSi PR01778. KIMPTPASE.
PR00700. PRTYPHPHTASE.
SMARTi SM00194. PTPc. 1 hit.
[Graphical view ]
SUPFAMi SSF52799. SSF52799. 1 hit.
PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of two alternatively spliced transcripts of STEP: a subfamily of brain-enriched protein tyrosine phosphatases."
    Sharma E., Zhao F., Bult A., Lombroso P.J.
    Brain Res. Mol. Brain Res. 32:87-93(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS STEP20; STEP38; STEP46 AND STEP61).
    Strain: BALB/c.
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM STEP61).
    Strain: C57BL/6J.
    Tissue: Hypothalamus.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM STEP61).
    Strain: C57BL/6.
    Tissue: Brain.
  5. "STEP61: a member of a family of brain-enriched PTPs is localized to the endoplasmic reticulum."
    Bult A., Zhao F., Dirkx R. Jr., Sharma E., Lukacsi E., Solimena M., Naegele J.R., Lombroso P.J.
    J. Neurosci. 16:7821-7831(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiPTN5_MOUSE
AccessioniPrimary (citable) accession number: P54830
Secondary accession number(s): Q64694, Q8CAN0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: February 10, 2009
Last modified: July 9, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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