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Protein

Tyrosine-protein phosphatase non-receptor type 5

Gene

PTPN5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May regulate the activity of several effector molecules involved in synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors.1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei461SubstrateBy similarity1
Active sitei496Phosphocysteine intermediatePROSITE-ProRule annotation1
Binding sitei540SubstrateBy similarity1

GO - Molecular functioni

  • phosphotyrosine binding Source: UniProtKB
  • protein tyrosine phosphatase activity Source: UniProtKB

GO - Biological processi

  • protein dephosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Enzyme and pathway databases

BioCyciZFISH:HS03338-MONOMER.
BRENDAi3.1.3.48. 2681.
SIGNORiP54829.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein phosphatase non-receptor type 5 (EC:3.1.3.48)
Alternative name(s):
Neural-specific protein-tyrosine phosphatase
Striatum-enriched protein-tyrosine phosphatase
Short name:
STEP
Gene namesi
Name:PTPN5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:9657. PTPN5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei88 – 108HelicalSequence analysisAdd BLAST21
Transmembranei146 – 166HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi84867.
OpenTargetsiENSG00000110786.
PharmGKBiPA34001.

Chemistry databases

ChEMBLiCHEMBL2007628.

Polymorphism and mutation databases

BioMutaiPTPN5.
DMDMi317373540.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003636571 – 565Tyrosine-protein phosphatase non-receptor type 5Add BLAST565

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei245Phosphoserine; by PKA1 Publication1
Modified residuei255Phosphothreonine; by MAPK1 Publication1
Modified residuei268Phosphoserine; by MAPK1 Publication1

Post-translational modificationi

Phosphorylation at Ser-245 by PKA deactivates PTPN5. Phosphorylation at Thr-255 and Ser-268 by MAPKs stabilizes the phosphatase, dephosphorylation of these sites results in ubiquitin-mediated degradation of the active phosphatase.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP54829.
PeptideAtlasiP54829.
PRIDEiP54829.

PTM databases

DEPODiP54829.
iPTMnetiP54829.
PhosphoSitePlusiP54829.

Expressioni

Gene expression databases

BgeeiENSG00000110786.
CleanExiHS_PTPN5.
ExpressionAtlasiP54829. baseline and differential.
GenevisibleiP54829. HS.

Organism-specific databases

HPAiHPA031014.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CXCL9Q073253EBI-1220572,EBI-3911467
TERF1P542742EBI-1220572,EBI-710997
TMEM51Q9BSA03EBI-1220572,EBI-10297449
TMEM51Q9NW973EBI-1220572,EBI-726044
VTI1BQ9UEU03EBI-1220572,EBI-723716

GO - Molecular functioni

  • phosphotyrosine binding Source: UniProtKB

Protein-protein interaction databases

BioGridi124312. 16 interactors.
IntActiP54829. 14 interactors.
STRINGi9606.ENSP00000351342.

Chemistry databases

BindingDBiP54829.

Structurei

Secondary structure

1565
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi287 – 293Combined sources7
Helixi297 – 304Combined sources8
Helixi314 – 316Combined sources3
Helixi322 – 325Combined sources4
Helixi335 – 337Combined sources3
Beta strandi338 – 340Combined sources3
Turni349 – 352Combined sources4
Beta strandi355 – 359Combined sources5
Helixi362 – 364Combined sources3
Beta strandi368 – 373Combined sources6
Helixi377 – 379Combined sources3
Helixi380 – 390Combined sources11
Beta strandi394 – 398Combined sources5
Helixi401 – 403Combined sources3
Turni404 – 406Combined sources3
Beta strandi414 – 419Combined sources6
Beta strandi422 – 431Combined sources10
Beta strandi433 – 443Combined sources11
Beta strandi448 – 456Combined sources9
Helixi465 – 467Combined sources3
Helixi468 – 484Combined sources17
Beta strandi492 – 500Combined sources9
Helixi501 – 519Combined sources19
Helixi524 – 534Combined sources11
Helixi542 – 559Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BIJX-ray2.05A282-563[»]
2BV5X-ray1.80A280-561[»]
2CJZX-ray1.70A282-563[»]
ProteinModelPortaliP54829.
SMRiP54829.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP54829.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini300 – 555Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd BLAST256

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni496 – 502Substrate binding7

Sequence similaritiesi

Contains 1 tyrosine-protein phosphatase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0789. Eukaryota.
COG5599. LUCA.
GeneTreeiENSGT00770000120452.
HOGENOMiHOG000294188.
HOVERGENiHBG001594.
InParanoidiP54829.
KOiK18018.
OMAiFTYSEWT.
OrthoDBiEOG091G0HLB.
PhylomeDBiP54829.
TreeFamiTF331016.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR000242. PTPase_domain.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR008356. Tyr_Pase_KIM-con.
IPR016334. Tyr_Pase_rcpt_R/non-rcpt_5.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PfamiPF00102. Y_phosphatase. 1 hit.
[Graphical view]
PIRSFiPIRSF001997. PTPRR. 1 hit.
PRINTSiPR01778. KIMPTPASE.
PR00700. PRTYPHPHTASE.
SMARTiSM00194. PTPc. 1 hit.
SM00404. PTPc_motif. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P54829-1) [UniParc]FASTAAdd to basket
Also known as: STEP61

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNYEGARSER ENHAADDSEG GALDMCCSER LPGLPQPIVM EALDEAEGLQ
60 70 80 90 100
DSQREMPPPP PPSPPSDPAQ KPPPRGAGSH SLTVRSSLCL FAASQFLLAC
110 120 130 140 150
GVLWFSGYGH IWSQNATNLV SSLLTLLKQL EPTAWLDSGT WGVPSLLLVF
160 170 180 190 200
LSVGLVLVTT LVWHLLRTPP EPPTPLPPED RRQSVSRQPS FTYSEWMEEK
210 220 230 240 250
IEDDFLDLDP VPETPVFDCV MDIKPEADPT SLTVKSMGLQ ERRGSNVSLT
260 270 280 290 300
LDMCTPGCNE EGFGYLMSPR EESAREYLLS ASRVLQAEEL HEKALDPFLL
310 320 330 340 350
QAEFFEIPMN FVDPKEYDIP GLVRKNRYKT ILPNPHSRVC LTSPDPDDPL
360 370 380 390 400
SSYINANYIR GYGGEEKVYI ATQGPIVSTV ADFWRMVWQE HTPIIVMITN
410 420 430 440 450
IEEMNEKCTE YWPEEQVAYD GVEITVQKVI HTEDYRLRLI SLKSGTEERG
460 470 480 490 500
LKHYWFTSWP DQKTPDRAPP LLHLVREVEE AAQQEGPHCA PIIVHCSAGI
510 520 530 540 550
GRTGCFIATS ICCQQLRQEG VVDILKTTCQ LRQDRGGMIQ TCEQYQFVHH
560
VMSLYEKQLS HQSPE
Length:565
Mass (Da):63,538
Last modified:January 11, 2011 - v4
Checksum:iDE32BEFFEFF7C494
GO
Isoform 2 (identifier: P54829-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     98-129: Missing.

Note: No experimental confirmation available.
Show »
Length:533
Mass (Da):60,035
Checksum:iA27BA66C3292C132
GO
Isoform 3 (identifier: P54829-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-24: Missing.

Note: No experimental confirmation available.
Show »
Length:541
Mass (Da):60,961
Checksum:iFE808E1908C2AB58
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti32 – 34PGL → LGR in AAA87555 (PubMed:7490079).Curated3
Sequence conflicti104W → R in BAC03548 (PubMed:14702039).Curated1
Sequence conflicti107G → A in CAD38632 (PubMed:17974005).Curated1
Sequence conflicti110H → D in CAD38632 (PubMed:17974005).Curated1
Sequence conflicti111I → M in AAA87555 (PubMed:7490079).Curated1
Sequence conflicti134A → S in AAA87555 (PubMed:7490079).Curated1
Sequence conflicti145S → G in AAA87555 (PubMed:7490079).Curated1
Sequence conflicti162V → L in CAD38632 (PubMed:17974005).Curated1
Sequence conflicti313D → V in AAA87555 (PubMed:7490079).Curated1
Sequence conflicti322 – 323LV → RC in AAA87555 (PubMed:7490079).Curated2
Sequence conflicti541T → H in AAA87555 (PubMed:7490079).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_054369170P → A.2 PublicationsCorresponds to variant rs4757707dbSNPEnsembl.1
Natural variantiVAR_054370561H → R.Corresponds to variant rs11024773dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0545611 – 24Missing in isoform 3. 1 PublicationAdd BLAST24
Alternative sequenceiVSP_04265498 – 129Missing in isoform 2. 1 PublicationAdd BLAST32

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK090923 mRNA. Translation: BAC03548.1.
AK127312 mRNA. Translation: BAG54479.1.
AK295604 mRNA. Translation: BAH12122.1.
AK316270 mRNA. Translation: BAH14641.1.
AL832541 mRNA. Translation: CAD38632.2.
AC103974 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW68363.1.
CH471064 Genomic DNA. Translation: EAW68365.1.
CH471064 Genomic DNA. Translation: EAW68367.1.
BC064807 mRNA. Translation: AAH64807.1.
U27831 mRNA. Translation: AAA87555.1.
CCDSiCCDS41626.1. [P54829-2]
CCDS60746.1. [P54829-3]
CCDS7845.1. [P54829-1]
RefSeqiNP_001035059.1. NM_001039970.1. [P54829-2]
NP_001265165.1. NM_001278236.1. [P54829-2]
NP_001265167.1. NM_001278238.1. [P54829-3]
NP_001265168.1. NM_001278239.1.
NP_008837.1. NM_006906.1. [P54829-1]
NP_116170.3. NM_032781.3. [P54829-1]
UniGeneiHs.79092.

Genome annotation databases

EnsembliENST00000358540; ENSP00000351342; ENSG00000110786. [P54829-1]
ENST00000396168; ENSP00000379471; ENSG00000110786. [P54829-3]
ENST00000396170; ENSP00000379473; ENSG00000110786. [P54829-2]
GeneIDi84867.
KEGGihsa:84867.
UCSCiuc001mpd.5. human. [P54829-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK090923 mRNA. Translation: BAC03548.1.
AK127312 mRNA. Translation: BAG54479.1.
AK295604 mRNA. Translation: BAH12122.1.
AK316270 mRNA. Translation: BAH14641.1.
AL832541 mRNA. Translation: CAD38632.2.
AC103974 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW68363.1.
CH471064 Genomic DNA. Translation: EAW68365.1.
CH471064 Genomic DNA. Translation: EAW68367.1.
BC064807 mRNA. Translation: AAH64807.1.
U27831 mRNA. Translation: AAA87555.1.
CCDSiCCDS41626.1. [P54829-2]
CCDS60746.1. [P54829-3]
CCDS7845.1. [P54829-1]
RefSeqiNP_001035059.1. NM_001039970.1. [P54829-2]
NP_001265165.1. NM_001278236.1. [P54829-2]
NP_001265167.1. NM_001278238.1. [P54829-3]
NP_001265168.1. NM_001278239.1.
NP_008837.1. NM_006906.1. [P54829-1]
NP_116170.3. NM_032781.3. [P54829-1]
UniGeneiHs.79092.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BIJX-ray2.05A282-563[»]
2BV5X-ray1.80A280-561[»]
2CJZX-ray1.70A282-563[»]
ProteinModelPortaliP54829.
SMRiP54829.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124312. 16 interactors.
IntActiP54829. 14 interactors.
STRINGi9606.ENSP00000351342.

Chemistry databases

BindingDBiP54829.
ChEMBLiCHEMBL2007628.

PTM databases

DEPODiP54829.
iPTMnetiP54829.
PhosphoSitePlusiP54829.

Polymorphism and mutation databases

BioMutaiPTPN5.
DMDMi317373540.

Proteomic databases

PaxDbiP54829.
PeptideAtlasiP54829.
PRIDEiP54829.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000358540; ENSP00000351342; ENSG00000110786. [P54829-1]
ENST00000396168; ENSP00000379471; ENSG00000110786. [P54829-3]
ENST00000396170; ENSP00000379473; ENSG00000110786. [P54829-2]
GeneIDi84867.
KEGGihsa:84867.
UCSCiuc001mpd.5. human. [P54829-1]

Organism-specific databases

CTDi84867.
DisGeNETi84867.
GeneCardsiPTPN5.
HGNCiHGNC:9657. PTPN5.
HPAiHPA031014.
MIMi176879. gene.
neXtProtiNX_P54829.
OpenTargetsiENSG00000110786.
PharmGKBiPA34001.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0789. Eukaryota.
COG5599. LUCA.
GeneTreeiENSGT00770000120452.
HOGENOMiHOG000294188.
HOVERGENiHBG001594.
InParanoidiP54829.
KOiK18018.
OMAiFTYSEWT.
OrthoDBiEOG091G0HLB.
PhylomeDBiP54829.
TreeFamiTF331016.

Enzyme and pathway databases

BioCyciZFISH:HS03338-MONOMER.
BRENDAi3.1.3.48. 2681.
SIGNORiP54829.

Miscellaneous databases

EvolutionaryTraceiP54829.
GeneWikiiPTPN5.
GenomeRNAii84867.
PROiP54829.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000110786.
CleanExiHS_PTPN5.
ExpressionAtlasiP54829. baseline and differential.
GenevisibleiP54829. HS.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR000242. PTPase_domain.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR008356. Tyr_Pase_KIM-con.
IPR016334. Tyr_Pase_rcpt_R/non-rcpt_5.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PfamiPF00102. Y_phosphatase. 1 hit.
[Graphical view]
PIRSFiPIRSF001997. PTPRR. 1 hit.
PRINTSiPR01778. KIMPTPASE.
PR00700. PRTYPHPHTASE.
SMARTiSM00194. PTPc. 1 hit.
SM00404. PTPc_motif. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTN5_HUMAN
AccessioniPrimary (citable) accession number: P54829
Secondary accession number(s): B3KXG7
, B7Z386, B7ZAF5, D3DQY7, Q6P1Z2, Q8N2A1, Q8NDP8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 11, 2011
Last modified: November 30, 2016
This is version 150 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-25 is the initiator.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.