Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Lens fiber membrane intrinsic protein

Gene

Lim2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Present in the thicker 16-17 nm junctions of mammalian lens fiber cells, where it may contribute to cell junctional organization. Acts as a receptor for calmodulin. May play an important role in both lens development and cataractogenesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Eye lens protein

Protein family/group databases

TCDBi8.A.16.3.1. the ca(+) channel auxiliary subunit Gama1-Gama8 (ccaGama) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Lens fiber membrane intrinsic protein
Alternative name(s):
MP17
MP18
MP19
MP20
Gene namesi
Name:Lim2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi621482. Lim2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 33CytoplasmicSequence analysis
Transmembranei4 – 2421HelicalSequence analysisAdd
BLAST
Topological domaini25 – 6642ExtracellularSequence analysisAdd
BLAST
Transmembranei67 – 8721HelicalSequence analysisAdd
BLAST
Topological domaini88 – 9811CytoplasmicSequence analysisAdd
BLAST
Transmembranei99 – 11921HelicalSequence analysisAdd
BLAST
Topological domaini120 – 14021ExtracellularSequence analysisAdd
BLAST
Transmembranei141 – 16121HelicalSequence analysisAdd
BLAST
Topological domaini162 – 17312CytoplasmicAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: RGD
  • vesicle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 173173Lens fiber membrane intrinsic proteinPRO_0000164666Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi43 – 431C-linked (Man)By similarity
Glycosylationi61 – 611C-linked (Man)By similarity
Glycosylationi62 – 621N-linked (GlcNAc...)Sequence analysis
Modified residuei170 – 1701PhosphoserineBy similarity
Modified residuei171 – 1711PhosphothreonineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP54825.

Expressioni

Tissue specificityi

Eye lens specific.

Gene expression databases

GenevisibleiP54825. RN.

Interactioni

Subunit structurei

Seems to be associated with itself or another lens membrane component via disulfide bonds.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023858.

Family & Domainsi

Sequence similaritiesi

Belongs to the PMP-22/EMP/MP20 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IN7P. Eukaryota.
ENOG4111H51. LUCA.
GeneTreeiENSGT00510000046994.
HOGENOMiHOG000116021.
HOVERGENiHBG003671.
InParanoidiP54825.
OMAiYCLGNKC.
OrthoDBiEOG7Z69DM.
PhylomeDBiP54825.
TreeFamiTF330587.

Family and domain databases

InterProiIPR003935. LMIP.
IPR004031. PMP22/EMP/MP20/Claudin.
IPR004032. PMP22_EMP_MP20.
[Graphical view]
PfamiPF00822. PMP22_Claudin. 1 hit.
[Graphical view]
PRINTSiPR01453. EPMEMFAMILY.
PR01457. LENSMEMPROT.
PROSITEiPS01221. PMP22_1. 1 hit.
PS01222. PMP22_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54825-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYSFMGGGLF CAWVGTILLV VATATDHWMQ YRLSGSFAHQ GLWRYCLGNK
60 70 80 90 100
CFLQTESIAY WNATRAFMIL SALCATSGII MGVLAFAQQS TFTRLSRPFS
110 120 130 140 150
AGIMFFASTL FVLLALAIYT GVTVSFLGRR FGDWRFSWSY ILGWVALLMT
160 170
FFAGIFYMCA YRMHECRRLS TPR
Length:173
Mass (Da):19,637
Last modified:October 1, 1996 - v1
Checksum:iC41FA9D76618D57F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti28 – 281W → Q AA sequence (PubMed:2584203).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S55224 mRNA. Translation: AAB25334.1.
M87053 mRNA. Translation: AAA41631.1.
L04191 mRNA. Translation: AAB02792.1.
PIRiA49182.
RefSeqiNP_446223.1. NM_053771.1.
UniGeneiRn.9688.

Genome annotation databases

EnsembliENSRNOT00000023858; ENSRNOP00000023858; ENSRNOG00000017681.
GeneIDi114903.
KEGGirno:114903.
UCSCiRGD:621482. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S55224 mRNA. Translation: AAB25334.1.
M87053 mRNA. Translation: AAA41631.1.
L04191 mRNA. Translation: AAB02792.1.
PIRiA49182.
RefSeqiNP_446223.1. NM_053771.1.
UniGeneiRn.9688.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023858.

Protein family/group databases

TCDBi8.A.16.3.1. the ca(+) channel auxiliary subunit Gama1-Gama8 (ccaGama) family.

Proteomic databases

PaxDbiP54825.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000023858; ENSRNOP00000023858; ENSRNOG00000017681.
GeneIDi114903.
KEGGirno:114903.
UCSCiRGD:621482. rat.

Organism-specific databases

CTDi3982.
RGDi621482. Lim2.

Phylogenomic databases

eggNOGiENOG410IN7P. Eukaryota.
ENOG4111H51. LUCA.
GeneTreeiENSGT00510000046994.
HOGENOMiHOG000116021.
HOVERGENiHBG003671.
InParanoidiP54825.
OMAiYCLGNKC.
OrthoDBiEOG7Z69DM.
PhylomeDBiP54825.
TreeFamiTF330587.

Miscellaneous databases

NextBioi618935.
PROiP54825.

Gene expression databases

GenevisibleiP54825. RN.

Family and domain databases

InterProiIPR003935. LMIP.
IPR004031. PMP22/EMP/MP20/Claudin.
IPR004032. PMP22_EMP_MP20.
[Graphical view]
PfamiPF00822. PMP22_Claudin. 1 hit.
[Graphical view]
PRINTSiPR01453. EPMEMFAMILY.
PR01457. LENSMEMPROT.
PROSITEiPS01221. PMP22_1. 1 hit.
PS01222. PMP22_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and expression of a major rat lens membrane protein, MP20."
    Kumar N.M., Jarvis L.J., Tenbroek E., Louis C.F.
    Exp. Eye Res. 56:35-43(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lens.
  2. "The human lens fiber-cell intrinsic membrane protein MP19 gene: isolation and sequence analysis."
    Church R.L., Wang J.H.
    Curr. Eye Res. 12:1057-1065(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lens.
  3. "Identification of an 18,000-dalton protein in mammalian lens fiber cell membranes."
    Louis C.F., Hur K.C., Galvan A.C., Tenbroek E.M., Jarvis L.J., Eccleston E.D., Howard J.B.
    J. Biol. Chem. 264:19967-19973(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-31.
    Tissue: Lens.

Entry informationi

Entry nameiLMIP_RAT
AccessioniPrimary (citable) accession number: P54825
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: February 17, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.