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Protein

Probable ATP-dependent RNA helicase DDX6

Gene

Ddx6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

In the process of mRNA degradation, plays a role in mRNA decapping (By similarity). Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degration of their transcripts (PubMed:26098573).By similarity1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.Curated

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi140 – 147ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

  • cytoplasmic mRNA processing body assembly Source: MGI
  • negative regulation of neuron differentiation Source: MGI
  • regulation of translation Source: GO_Central
  • RNA secondary structure unwinding Source: GO_Central
  • spermatogenesis Source: Ensembl
  • stem cell population maintenance Source: MGI
  • viral RNA genome packaging Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-430039. mRNA decay by 5' to 3' exoribonuclease.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase DDX6Curated (EC:3.6.4.13Curated)
Alternative name(s):
ATP-dependent RNA helicase p54
DEAD box protein 6
Oncogene RCK homolog
Gene namesi
Name:Ddx6
Synonyms:Hlr2, Rck
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:104976. Ddx6.

Subcellular locationi

  • CytoplasmP-body By similarity
  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Upon cellular stress, relocalizes to stress granules.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000549841 – 483Probable ATP-dependent RNA helicase DDX6Add BLAST483

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei36PhosphothreonineBy similarity1

Post-translational modificationi

Sumoylated.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP54823.
MaxQBiP54823.
PaxDbiP54823.
PeptideAtlasiP54823.
PRIDEiP54823.

PTM databases

iPTMnetiP54823.
PhosphoSitePlusiP54823.
SwissPalmiP54823.

Expressioni

Developmental stagei

Abundant expression in growing oocytes, levels decline in primary and secondary oocytes, and degradation appears to be complete by the mid-late two-cell stage.1 Publication

Gene expression databases

BgeeiENSMUSG00000032097.
CleanExiMM_DDX6.
GenevisibleiP54823. MM.

Interactioni

Subunit structurei

Forms a complex with DCP1A, DCP2, EDC3 and EDC4/HEDLS (By similarity). Interacts with LIMD1, WTIP and AJUBA (By similarity). Interacts with APOBEC3G in an RNA-dependent manner (By similarity). Interacts with RC3H1 (PubMed:20639877). Interacts with ATXN2L (By similarity). Interacts with MCRIP1 (By similarity). Interacts with MCRIP2 (By similarity). Interacts with NUFIP2 (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199087. 2 interactors.
IntActiP54823. 6 interactors.
MINTiMINT-4093002.
STRINGi10090.ENSMUSP00000128421.

Structurei

3D structure databases

ProteinModelPortaliP54823.
SMRiP54823.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini127 – 298Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST172
Domaini308 – 468Helicase C-terminalPROSITE-ProRule annotationAdd BLAST161

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi96 – 124Q motifAdd BLAST29
Motifi246 – 249DEAD box4

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0326. Eukaryota.
ENOG410XRAZ. LUCA.
GeneTreeiENSGT00860000133781.
HOGENOMiHOG000268797.
HOVERGENiHBG106685.
InParanoidiP54823.
KOiK12614.
OMAiIYQKVQV.
OrthoDBiEOG091G0623.
PhylomeDBiP54823.
TreeFamiTF300440.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54823-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTARTENPV IMGLSSQNGQ LRGPVKASAG PGGGGTQPQP QLNQLKNTST
60 70 80 90 100
INNGTPQQAQ SMAATIKPGD DWKKTLKLPP KDLRIKTSDV TSTKGNEFED
110 120 130 140 150
YCLKRELLMG IFEMGWEKPS PIQEESIPIA LSGRDILARA KNGTGKSGAY
160 170 180 190 200
LIPLLERLDL KKDNIQAMVI VPTRELALQV SQICIQVSKH MGGAKVMATT
210 220 230 240 250
GGTNLRDDIM RLDDTVHVVI ATPGRILDLI KKGVAKVDHV QMIVLDEADK
260 270 280 290 300
LLSQDFVQIM EDIILTLPKN RQILLYSATF PLSVQKFMNS HLQKPYEINL
310 320 330 340 350
MEELTLKGVT QYYAYVTERQ KVHCLNTLFS RLQINQSIIF CNSSQRVELL
360 370 380 390 400
AKKISQLGYS CFYIHAKMRQ EHRNRVFHDF RNGLCRNLVC TDLFTRGIDI
410 420 430 440 450
QAVNVVINFD FPKLAETYLH RIGRSGRFGH LGLAINLITY DDRFNLKSIE
460 470 480
EQLGTEIKPI PSNIDKSLYV AEYHSEPAED EKP
Length:483
Mass (Da):54,192
Last modified:October 1, 1996 - v1
Checksum:i9AD22D171F8BC14D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti38 – 42PQPQL → TQQQM in AAB94769 (PubMed:9883581).Curated5
Sequence conflicti202G → P in AAB94769 (PubMed:9883581).Curated1
Sequence conflicti241Q → R in BAC35670 (PubMed:15489334).Curated1
Sequence conflicti311Q → E in BAC35670 (PubMed:15489334).Curated1
Sequence conflicti381R → E in AAB94769 (PubMed:9883581).Curated1
Sequence conflicti407I → M in BAC35670 (PubMed:15489334).Curated1
Sequence conflicti422I → V in AAB94769 (PubMed:9883581).Curated1
Sequence conflicti478A → V in AAB94769 (PubMed:9883581).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50494 mRNA. Translation: BAA09088.1.
AK054144 mRNA. Translation: BAC35670.1.
AK148483 mRNA. Translation: BAE28578.1.
BC021452 mRNA. Translation: AAH21452.1.
AF038995 mRNA. Translation: AAB94769.1.
CCDSiCCDS23116.1.
RefSeqiNP_001104296.1. NM_001110826.1.
NP_031867.1. NM_007841.4.
NP_851841.2. NM_181324.3.
XP_011240693.1. XM_011242391.2.
XP_011240694.1. XM_011242392.2.
XP_017168613.1. XM_017313124.1.
UniGeneiMm.267061.

Genome annotation databases

EnsembliENSMUST00000170489; ENSMUSP00000128421; ENSMUSG00000032097.
GeneIDi13209.
KEGGimmu:13209.
UCSCiuc009pdy.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50494 mRNA. Translation: BAA09088.1.
AK054144 mRNA. Translation: BAC35670.1.
AK148483 mRNA. Translation: BAE28578.1.
BC021452 mRNA. Translation: AAH21452.1.
AF038995 mRNA. Translation: AAB94769.1.
CCDSiCCDS23116.1.
RefSeqiNP_001104296.1. NM_001110826.1.
NP_031867.1. NM_007841.4.
NP_851841.2. NM_181324.3.
XP_011240693.1. XM_011242391.2.
XP_011240694.1. XM_011242392.2.
XP_017168613.1. XM_017313124.1.
UniGeneiMm.267061.

3D structure databases

ProteinModelPortaliP54823.
SMRiP54823.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199087. 2 interactors.
IntActiP54823. 6 interactors.
MINTiMINT-4093002.
STRINGi10090.ENSMUSP00000128421.

PTM databases

iPTMnetiP54823.
PhosphoSitePlusiP54823.
SwissPalmiP54823.

Proteomic databases

EPDiP54823.
MaxQBiP54823.
PaxDbiP54823.
PeptideAtlasiP54823.
PRIDEiP54823.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000170489; ENSMUSP00000128421; ENSMUSG00000032097.
GeneIDi13209.
KEGGimmu:13209.
UCSCiuc009pdy.2. mouse.

Organism-specific databases

CTDi1656.
MGIiMGI:104976. Ddx6.

Phylogenomic databases

eggNOGiKOG0326. Eukaryota.
ENOG410XRAZ. LUCA.
GeneTreeiENSGT00860000133781.
HOGENOMiHOG000268797.
HOVERGENiHBG106685.
InParanoidiP54823.
KOiK12614.
OMAiIYQKVQV.
OrthoDBiEOG091G0623.
PhylomeDBiP54823.
TreeFamiTF300440.

Enzyme and pathway databases

ReactomeiR-MMU-430039. mRNA decay by 5' to 3' exoribonuclease.

Miscellaneous databases

PROiP54823.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032097.
CleanExiMM_DDX6.
GenevisibleiP54823. MM.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDDX6_MOUSE
AccessioniPrimary (citable) accession number: P54823
Secondary accession number(s): O54979, Q3UFI3, Q8BW68
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.