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P54823 (DDX6_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable ATP-dependent RNA helicase DDX6

EC=3.6.4.13
Alternative name(s):
ATP-dependent RNA helicase p54
DEAD box protein 6
Oncogene RCK homolog
Gene names
Name:Ddx6
Synonyms:Hlr2, Rck
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length483 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

In the process of mRNA degradation, may play a role in mRNA decapping.

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

Forms a complex with DCP1A, DCP2, EDC3 and EDC4/HEDLS By similarity. Interacts with LIMD1, WTIP and AJUBA By similarity. Interacts with APOBEC3G in an RNA-dependent manner By similarity. Interacts with RC3H1. Ref.5

Subcellular location

CytoplasmP-body By similarity.

Developmental stage

Abundant expression in growing oocytes, levels decline in primary and secondary oocytes, and degradation appears to be complete by the mid-late two-cell stage. Ref.4

Sequence similarities

Belongs to the DEAD box helicase family. DDX6/DHH1 subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 483483Probable ATP-dependent RNA helicase DDX6
PRO_0000054984

Regions

Domain127 – 298172Helicase ATP-binding
Domain308 – 468161Helicase C-terminal
Nucleotide binding140 – 1478ATP By similarity
Motif96 – 12429Q motif
Motif246 – 2494DEAD box

Amino acid modifications

Modified residue361Phosphothreonine By similarity

Experimental info

Sequence conflict38 – 425PQPQL → TQQQM in AAB94769. Ref.4
Sequence conflict2021G → P in AAB94769. Ref.4
Sequence conflict2411Q → R in BAC35670. Ref.3
Sequence conflict3111Q → E in BAC35670. Ref.3
Sequence conflict3811R → E in AAB94769. Ref.4
Sequence conflict4071I → M in BAC35670. Ref.3
Sequence conflict4221I → V in AAB94769. Ref.4
Sequence conflict4781A → V in AAB94769. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P54823 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 9AD22D171F8BC14D

FASTA48354,192
        10         20         30         40         50         60 
MSTARTENPV IMGLSSQNGQ LRGPVKASAG PGGGGTQPQP QLNQLKNTST INNGTPQQAQ 

        70         80         90        100        110        120 
SMAATIKPGD DWKKTLKLPP KDLRIKTSDV TSTKGNEFED YCLKRELLMG IFEMGWEKPS 

       130        140        150        160        170        180 
PIQEESIPIA LSGRDILARA KNGTGKSGAY LIPLLERLDL KKDNIQAMVI VPTRELALQV 

       190        200        210        220        230        240 
SQICIQVSKH MGGAKVMATT GGTNLRDDIM RLDDTVHVVI ATPGRILDLI KKGVAKVDHV 

       250        260        270        280        290        300 
QMIVLDEADK LLSQDFVQIM EDIILTLPKN RQILLYSATF PLSVQKFMNS HLQKPYEINL 

       310        320        330        340        350        360 
MEELTLKGVT QYYAYVTERQ KVHCLNTLFS RLQINQSIIF CNSSQRVELL AKKISQLGYS 

       370        380        390        400        410        420 
CFYIHAKMRQ EHRNRVFHDF RNGLCRNLVC TDLFTRGIDI QAVNVVINFD FPKLAETYLH 

       430        440        450        460        470        480 
RIGRSGRFGH LGLAINLITY DDRFNLKSIE EQLGTEIKPI PSNIDKSLYV AEYHSEPAED 


EKP 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and expression of a murine cDNA homologous to the human RCK/P54, a lymphoma-linked chromosomal translocation junction gene on 11q23."
Seto M., Yamamoto K., Takahashi T., Ueda R.
Gene 166:293-296(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Spleen.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Oviduct and Pancreas.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Kidney.
[4]"RNA-binding proteins in mouse oocytes and embryos: expression of genes encoding Y box, DEAD box RNA helicase, and polyA binding proteins."
Paynton B.V.
Dev. Genet. 23:285-298(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 26-478, DEVELOPMENTAL STAGE.
Strain: CB6F1/J.
Tissue: Oocyte.
[5]"Roquin binds inducible costimulator mRNA and effectors of mRNA decay to induce microRNA-independent post-transcriptional repression."
Glasmacher E., Hoefig K.P., Vogel K.U., Rath N., Du L., Wolf C., Kremmer E., Wang X., Heissmeyer V.
Nat. Immunol. 11:725-733(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RC3H1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D50494 mRNA. Translation: BAA09088.1.
AK054144 mRNA. Translation: BAC35670.1.
AK148483 mRNA. Translation: BAE28578.1.
BC021452 mRNA. Translation: AAH21452.1.
AF038995 mRNA. Translation: AAB94769.1.
CCDSCCDS23116.1.
RefSeqNP_001104296.1. NM_001110826.1.
NP_031867.1. NM_007841.4.
NP_851841.2. NM_181324.3.
UniGeneMm.267061.

3D structure databases

ProteinModelPortalP54823.
SMRP54823. Positions 94-472.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199087. 2 interactions.
IntActP54823. 5 interactions.
MINTMINT-4093002.

PTM databases

PhosphoSiteP54823.

Proteomic databases

MaxQBP54823.
PaxDbP54823.
PRIDEP54823.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000170489; ENSMUSP00000128421; ENSMUSG00000032097.
GeneID13209.
KEGGmmu:13209.
UCSCuc009pdy.2. mouse.

Organism-specific databases

CTD1656.
MGIMGI:104976. Ddx6.

Phylogenomic databases

eggNOGCOG0513.
GeneTreeENSGT00740000115573.
HOGENOMHOG000268797.
HOVERGENHBG106685.
InParanoidP54823.
KOK12614.
OMALLQFHPK.
OrthoDBEOG7D85W7.
PhylomeDBP54823.
TreeFamTF300440.

Gene expression databases

BgeeP54823.
CleanExMM_DDX6.
GenevestigatorP54823.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio283376.
PROP54823.
SOURCESearch...

Entry information

Entry nameDDX6_MOUSE
AccessionPrimary (citable) accession number: P54823
Secondary accession number(s): O54979, Q3UFI3, Q8BW68
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 9, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot