P54810 (THIL_PARDE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetyl-CoA acetyltransferase
EC=2.3.1.9

Alternative name(s):
Acetoacetyl-CoA thiolase

Gene names

Name:

phaA

Organism

Paracoccus denitrificans

Taxonomic identifier

266 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Paracoccus

Protein attributes

Sequence length	391 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2 acetyl-CoA = CoA + acetoacetyl-CoA.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 1/3.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the thiolase family.

Ontologies

Keywords
Biological process	PHB biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	poly-hydroxybutyrate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA C-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 391	391	Acetyl-CoA acetyltransferase		PRO_0000206460
Sites
	Active site	88	1	Acyl-thioester intermediate By similarity
	Active site	347	1	Proton acceptor By similarity
	Active site	377	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P54810 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: A6278FD632EC697B	FASTA	391	40,745
	10 20 30 40 50 60 MTKAVIVSAA RTPVGSFLGS FANLPAHELG AIVLKAVVER AGIDPSEVSE TILGQVLTAA 70 80 90 100 110 120 QGQNPARQAH IKVGLPRESA AWVINQVCGS GLRTVALAAQ QVLLGDARIV VAGGQESMSL 130 140 150 160 170 180 APHAAYIAPG QKMGDMKMLD TMIKDGLWDA FNDYHMGTTA ENVAGKWEIS RAEQDQFAVA 190 200 210 220 230 240 SQNKAEAAQK AGKFADEIVP VTIKSRKGET VVDADEYIRH GATLEAMENV RPAFSKEGTV 250 260 270 280 290 300 TAGNASGLND GAAAVLVMTE DEAARRGLTP LARIASYATA GVDPQIMGTG PIPASRKALE 310 320 330 340 350 360 KAGWSVGDLD LVEANEAFAA QAIAVNRDMG WDPSIVNVNG GAIAIGHPIG ASGCRILNTL 370 380 390 LFEMQRRDAK KGLATLCIGG GMGVALCLER P

References

[1]	"Analysis of beta-ketothiolase and acetoacetyl-CoA reductase genes of a methylotrophic bacterium, Paracoccus denitrificans, and their expression in Escherichia coli." Yabutani T., Maehara A., Ueda S., Yamane T. FEMS Microbiol. Lett. 133:85-90(1995) [PubMed: 8566717] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D49362 Genomic DNA. Translation: BAA08357.1.
3D structure databases
ProteinModelPortal	P54810.
SMR	P54810. Positions 5-389.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002155. Thiolase. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. IPR020615. Thiolase_acyl_enz_int_AS. IPR020610. Thiolase_AS. IPR020617. Thiolase_C. IPR020613. Thiolase_CS. IPR020616. Thiolase_N. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 4 hits.
PANTHER	PTHR18919. Thiolase. 1 hit.
Pfam	PF02803. Thiolase_C. 1 hit. PF00108. Thiolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAM	SSF53901. Thiolase-like. 2 hits.
TIGRFAMs	TIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITE	PS00098. THIOLASE_1. 1 hit. PS00737. THIOLASE_2. 1 hit. PS00099. THIOLASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THIL_PARDE

Accession

Primary (citable) accession number: P54810

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 31, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program