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Protein

Origin recognition complex subunit 4

Gene

ORC4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the origin recognition complex (ORC) that binds origins of replication. It has a role in both chromosomal replication and mating type transcriptional silencing. Binds to the ARS consensus sequence (ACS) of origins of replication.

GO - Molecular functioni

  • DNA replication origin binding Source: SGD

GO - Biological processi

  • chromatin silencing at silent mating-type cassette Source: SGD
  • DNA replication initiation Source: SGD
  • pre-replicative complex assembly involved in nuclear cell cycle DNA replication Source: SGD
Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34291-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Origin recognition complex subunit 4
Alternative name(s):
Origin recognition complex 56 kDa subunit
Gene namesi
Name:ORC4
Ordered Locus Names:YPR162C
ORF Names:P9325.5
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XVI

Organism-specific databases

CYGDiYPR162c.
EuPathDBiFungiDB:YPR162C.
SGDiS000006366. ORC4.

Subcellular locationi

GO - Cellular componenti

  • DNA replication preinitiation complex Source: SGD
  • nuclear origin of replication recognition complex Source: SGD
  • nuclear pre-replicative complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 529529Origin recognition complex subunit 4PRO_0000127091Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei9 – 91Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP54791.
PaxDbiP54791.

Expressioni

Gene expression databases

GenevestigatoriP54791.

Interactioni

Subunit structurei

Component of the origin recognition complex (ORC) composed of at least ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6.

Binary interactionsi

WithEntry#Exp.IntActNotes
ORC1P547844EBI-12580,EBI-12568
ORC5P508745EBI-12580,EBI-12584

Protein-protein interaction databases

BioGridi36335. 23 interactions.
DIPiDIP-2287N.
IntActiP54791. 15 interactions.
MINTiMINT-669611.
STRINGi4932.YPR162C.

Structurei

3D structure databases

ProteinModelPortaliP54791.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ORC4 family.Curated

Phylogenomic databases

eggNOGiNOG289784.
GeneTreeiENSGT00390000016542.
HOGENOMiHOG000001008.
InParanoidiP54791.
KOiK02606.
OMAiCTTKLNI.
OrthoDBiEOG70610P.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR016527. ORC4.
IPR027417. P-loop_NTPase.
[Graphical view]
PIRSFiPIRSF007858. ORC4. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P54791-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTISEARLSP QVNLLPIKRH SNEEVEETAA ILKKRTIDNE KCKDSDPGFG
60 70 80 90 100
SLQRRLLQQL YGTLPTDEKI IFTYLQDCQQ EIDRIIKQSI IQKESHSVIL
110 120 130 140 150
VGPRQSYKTY LLDYELSLLQ QSYKEQFITI RLNGFIHSEQ TAINGIATQL
160 170 180 190 200
EQQLQKIHGS EEKIDDTSLE TISSGSLTEV FEKILLLLDS TTKTRNEDSG
210 220 230 240 250
EVDRESITKI TVVFIFDEID TFAGPVRQTL LYNLFDMVEH SRVPVCIFGC
260 270 280 290 300
TTKLNILEYL EKRVKSRFSQ RVIYMPQIQN LDDMVDAVRN LLTVRSEISP
310 320 330 340 350
WVSQWNETLE KELSDPRSNL NRHIRMNFET FRSLPTLKNS IIPLVATSKN
360 370 380 390 400
FGSLCTAIKS CSFLDIYNKN QLSNNLTGRL QSLSDLELAI LISAARVALR
410 420 430 440 450
AKDGSFNFNL AYAEYEKMIK AINSRIPTVA PTTNVGTGQS TFSIDNTIKL
460 470 480 490 500
WLKKDVKNVW ENLVQLDFFT EKSAVGLRDN ATAAFYASNY QFQGTMIPFD
510 520
LRSYQMQIIL QELRRIIPKS NMYYSWTQL
Length:529
Mass (Da):60,705
Last modified:October 1, 1996 - v1
Checksum:iACF064F2C633F13F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34862 Genomic DNA. Translation: AAB38250.1.
U25840 Genomic DNA. Translation: AAB68149.1.
AY723871 Genomic DNA. Translation: AAU09788.1.
BK006949 Genomic DNA. Translation: DAA11579.1.
PIRiS59821.
RefSeqiNP_015488.1. NM_001184259.1.

Genome annotation databases

EnsemblFungiiYPR162C; YPR162C; YPR162C.
GeneIDi856291.
KEGGisce:YPR162C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34862 Genomic DNA. Translation: AAB38250.1.
U25840 Genomic DNA. Translation: AAB68149.1.
AY723871 Genomic DNA. Translation: AAU09788.1.
BK006949 Genomic DNA. Translation: DAA11579.1.
PIRiS59821.
RefSeqiNP_015488.1. NM_001184259.1.

3D structure databases

ProteinModelPortaliP54791.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36335. 23 interactions.
DIPiDIP-2287N.
IntActiP54791. 15 interactions.
MINTiMINT-669611.
STRINGi4932.YPR162C.

Proteomic databases

MaxQBiP54791.
PaxDbiP54791.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR162C; YPR162C; YPR162C.
GeneIDi856291.
KEGGisce:YPR162C.

Organism-specific databases

CYGDiYPR162c.
EuPathDBiFungiDB:YPR162C.
SGDiS000006366. ORC4.

Phylogenomic databases

eggNOGiNOG289784.
GeneTreeiENSGT00390000016542.
HOGENOMiHOG000001008.
InParanoidiP54791.
KOiK02606.
OMAiCTTKLNI.
OrthoDBiEOG70610P.

Enzyme and pathway databases

BioCyciYEAST:G3O-34291-MONOMER.

Miscellaneous databases

NextBioi981632.
PROiP54791.

Gene expression databases

GenevestigatoriP54791.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR016527. ORC4.
IPR027417. P-loop_NTPase.
[Graphical view]
PIRSFiPIRSF007858. ORC4. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The multidomain structure of Orc1p reveals similarity to regulators of DNA replication and transcriptional silencing."
    Bell S.P., Mitchell J., Leber J., Kobayashi R., Stillman B.
    Cell 83:563-568(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiORC4_YEAST
AccessioniPrimary (citable) accession number: P54791
Secondary accession number(s): D6W4G3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 27, 2015
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2170 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.