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P54791

- ORC4_YEAST

UniProt

P54791 - ORC4_YEAST

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Protein

Origin recognition complex subunit 4

Gene

ORC4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the origin recognition complex (ORC) that binds origins of replication. It has a role in both chromosomal replication and mating type transcriptional silencing. Binds to the ARS consensus sequence (ACS) of origins of replication.

GO - Molecular functioni

  1. DNA replication origin binding Source: SGD

GO - Biological processi

  1. chromatin silencing at silent mating-type cassette Source: SGD
  2. DNA replication initiation Source: SGD
  3. pre-replicative complex assembly involved in nuclear cell cycle DNA replication Source: SGD
Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34291-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Origin recognition complex subunit 4
Alternative name(s):
Origin recognition complex 56 kDa subunit
Gene namesi
Name:ORC4
Ordered Locus Names:YPR162C
ORF Names:P9325.5
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPR162c.
SGDiS000006366. ORC4.

Subcellular locationi

GO - Cellular componenti

  1. DNA replication preinitiation complex Source: SGD
  2. nuclear origin of replication recognition complex Source: SGD
  3. nuclear pre-replicative complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 529529Origin recognition complex subunit 4PRO_0000127091Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei9 – 91Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP54791.
PaxDbiP54791.

Expressioni

Gene expression databases

GenevestigatoriP54791.

Interactioni

Subunit structurei

Component of the origin recognition complex (ORC) composed of at least ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6.

Binary interactionsi

WithEntry#Exp.IntActNotes
ORC1P547844EBI-12580,EBI-12568
ORC5P508745EBI-12580,EBI-12584

Protein-protein interaction databases

BioGridi36335. 22 interactions.
DIPiDIP-2287N.
IntActiP54791. 15 interactions.
MINTiMINT-669611.
STRINGi4932.YPR162C.

Structurei

3D structure databases

ProteinModelPortaliP54791.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ORC4 family.Curated

Phylogenomic databases

eggNOGiNOG289784.
GeneTreeiENSGT00390000016542.
HOGENOMiHOG000001008.
InParanoidiP54791.
KOiK02606.
OMAiCTTKLNI.
OrthoDBiEOG70610P.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR016527. ORC4.
IPR027417. P-loop_NTPase.
[Graphical view]
PIRSFiPIRSF007858. ORC4. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P54791-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTISEARLSP QVNLLPIKRH SNEEVEETAA ILKKRTIDNE KCKDSDPGFG
60 70 80 90 100
SLQRRLLQQL YGTLPTDEKI IFTYLQDCQQ EIDRIIKQSI IQKESHSVIL
110 120 130 140 150
VGPRQSYKTY LLDYELSLLQ QSYKEQFITI RLNGFIHSEQ TAINGIATQL
160 170 180 190 200
EQQLQKIHGS EEKIDDTSLE TISSGSLTEV FEKILLLLDS TTKTRNEDSG
210 220 230 240 250
EVDRESITKI TVVFIFDEID TFAGPVRQTL LYNLFDMVEH SRVPVCIFGC
260 270 280 290 300
TTKLNILEYL EKRVKSRFSQ RVIYMPQIQN LDDMVDAVRN LLTVRSEISP
310 320 330 340 350
WVSQWNETLE KELSDPRSNL NRHIRMNFET FRSLPTLKNS IIPLVATSKN
360 370 380 390 400
FGSLCTAIKS CSFLDIYNKN QLSNNLTGRL QSLSDLELAI LISAARVALR
410 420 430 440 450
AKDGSFNFNL AYAEYEKMIK AINSRIPTVA PTTNVGTGQS TFSIDNTIKL
460 470 480 490 500
WLKKDVKNVW ENLVQLDFFT EKSAVGLRDN ATAAFYASNY QFQGTMIPFD
510 520
LRSYQMQIIL QELRRIIPKS NMYYSWTQL
Length:529
Mass (Da):60,705
Last modified:October 1, 1996 - v1
Checksum:iACF064F2C633F13F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34862 Genomic DNA. Translation: AAB38250.1.
U25840 Genomic DNA. Translation: AAB68149.1.
AY723871 Genomic DNA. Translation: AAU09788.1.
BK006949 Genomic DNA. Translation: DAA11579.1.
PIRiS59821.
RefSeqiNP_015488.1. NM_001184259.1.

Genome annotation databases

EnsemblFungiiYPR162C; YPR162C; YPR162C.
GeneIDi856291.
KEGGisce:YPR162C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34862 Genomic DNA. Translation: AAB38250.1 .
U25840 Genomic DNA. Translation: AAB68149.1 .
AY723871 Genomic DNA. Translation: AAU09788.1 .
BK006949 Genomic DNA. Translation: DAA11579.1 .
PIRi S59821.
RefSeqi NP_015488.1. NM_001184259.1.

3D structure databases

ProteinModelPortali P54791.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36335. 22 interactions.
DIPi DIP-2287N.
IntActi P54791. 15 interactions.
MINTi MINT-669611.
STRINGi 4932.YPR162C.

Proteomic databases

MaxQBi P54791.
PaxDbi P54791.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPR162C ; YPR162C ; YPR162C .
GeneIDi 856291.
KEGGi sce:YPR162C.

Organism-specific databases

CYGDi YPR162c.
SGDi S000006366. ORC4.

Phylogenomic databases

eggNOGi NOG289784.
GeneTreei ENSGT00390000016542.
HOGENOMi HOG000001008.
InParanoidi P54791.
KOi K02606.
OMAi CTTKLNI.
OrthoDBi EOG70610P.

Enzyme and pathway databases

BioCyci YEAST:G3O-34291-MONOMER.

Miscellaneous databases

NextBioi 981632.
PROi P54791.

Gene expression databases

Genevestigatori P54791.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR016527. ORC4.
IPR027417. P-loop_NTPase.
[Graphical view ]
PIRSFi PIRSF007858. ORC4. 1 hit.
SUPFAMi SSF52540. SSF52540. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The multidomain structure of Orc1p reveals similarity to regulators of DNA replication and transcriptional silencing."
    Bell S.P., Mitchell J., Leber J., Kobayashi R., Stillman B.
    Cell 83:563-568(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiORC4_YEAST
AccessioniPrimary (citable) accession number: P54791
Secondary accession number(s): D6W4G3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2170 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3