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Protein

Origin recognition complex subunit 4

Gene

ORC4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the origin recognition complex (ORC) that binds origins of replication. It has a role in both chromosomal replication and mating type transcriptional silencing. Binds to the ARS consensus sequence (ACS) of origins of replication.

Miscellaneous

Present with 2170 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • DNA replication origin binding Source: SGD

GO - Biological processi

  • chromatin silencing at silent mating-type cassette Source: SGD
  • DNA replication initiation Source: SGD
  • pre-replicative complex assembly involved in nuclear cell cycle DNA replication Source: SGD

Keywordsi

Molecular functionDNA-binding
Biological processDNA replication

Enzyme and pathway databases

BioCyciYEAST:G3O-34291-MONOMER.
ReactomeiR-SCE-68616. Assembly of the ORC complex at the origin of replication.
R-SCE-68949. Orc1 removal from chromatin.
R-SCE-68962. Activation of the pre-replicative complex.
R-SCE-69298. Association of licensing factors with the pre-replicative complex.

Names & Taxonomyi

Protein namesi
Recommended name:
Origin recognition complex subunit 4
Alternative name(s):
Origin recognition complex 56 kDa subunit
Gene namesi
Name:ORC4
Ordered Locus Names:YPR162C
ORF Names:P9325.5
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR162C.
SGDiS000006366. ORC4.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001270911 – 529Origin recognition complex subunit 4Add BLAST529

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP54791.
PRIDEiP54791.

PTM databases

iPTMnetiP54791.

Interactioni

Subunit structurei

Component of the origin recognition complex (ORC) composed of at least ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi36335. 306 interactors.
DIPiDIP-2287N.
IntActiP54791. 29 interactors.
MINTiMINT-669611.
STRINGi4932.YPR162C.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5UDBelectron microscopy3.90D1-529[»]
5V8Felectron microscopy3.90D1-529[»]
ProteinModelPortaliP54791.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ORC4 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000016542.
HOGENOMiHOG000001008.
InParanoidiP54791.
KOiK02606.
OMAiCTTKLNI.
OrthoDBiEOG092C1ZIR.

Family and domain databases

InterProiView protein in InterPro
IPR016527. ORC4.
IPR032705. ORC4_C.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF14629. ORC4_C. 1 hit.
PIRSFiPIRSF007858. ORC4. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P54791-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTISEARLSP QVNLLPIKRH SNEEVEETAA ILKKRTIDNE KCKDSDPGFG
60 70 80 90 100
SLQRRLLQQL YGTLPTDEKI IFTYLQDCQQ EIDRIIKQSI IQKESHSVIL
110 120 130 140 150
VGPRQSYKTY LLDYELSLLQ QSYKEQFITI RLNGFIHSEQ TAINGIATQL
160 170 180 190 200
EQQLQKIHGS EEKIDDTSLE TISSGSLTEV FEKILLLLDS TTKTRNEDSG
210 220 230 240 250
EVDRESITKI TVVFIFDEID TFAGPVRQTL LYNLFDMVEH SRVPVCIFGC
260 270 280 290 300
TTKLNILEYL EKRVKSRFSQ RVIYMPQIQN LDDMVDAVRN LLTVRSEISP
310 320 330 340 350
WVSQWNETLE KELSDPRSNL NRHIRMNFET FRSLPTLKNS IIPLVATSKN
360 370 380 390 400
FGSLCTAIKS CSFLDIYNKN QLSNNLTGRL QSLSDLELAI LISAARVALR
410 420 430 440 450
AKDGSFNFNL AYAEYEKMIK AINSRIPTVA PTTNVGTGQS TFSIDNTIKL
460 470 480 490 500
WLKKDVKNVW ENLVQLDFFT EKSAVGLRDN ATAAFYASNY QFQGTMIPFD
510 520
LRSYQMQIIL QELRRIIPKS NMYYSWTQL
Length:529
Mass (Da):60,705
Last modified:October 1, 1996 - v1
Checksum:iACF064F2C633F13F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34862 Genomic DNA. Translation: AAB38250.1.
U25840 Genomic DNA. Translation: AAB68149.1.
AY723871 Genomic DNA. Translation: AAU09788.1.
BK006949 Genomic DNA. Translation: DAA11579.1.
PIRiS59821.
RefSeqiNP_015488.1. NM_001184259.1.

Genome annotation databases

EnsemblFungiiYPR162C; YPR162C; YPR162C.
GeneIDi856291.
KEGGisce:YPR162C.

Similar proteinsi

Entry informationi

Entry nameiORC4_YEAST
AccessioniPrimary (citable) accession number: P54791
Secondary accession number(s): D6W4G3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 27, 2017
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names