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P54791

- ORC4_YEAST

UniProt

P54791 - ORC4_YEAST

Protein

Origin recognition complex subunit 4

Gene

ORC4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Component of the origin recognition complex (ORC) that binds origins of replication. It has a role in both chromosomal replication and mating type transcriptional silencing. Binds to the ARS consensus sequence (ACS) of origins of replication.

    GO - Molecular functioni

    1. DNA replication origin binding Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. chromatin silencing at silent mating-type cassette Source: SGD
    2. DNA replication initiation Source: SGD
    3. pre-replicative complex assembly involved in nuclear cell cycle DNA replication Source: SGD

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34291-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Origin recognition complex subunit 4
    Alternative name(s):
    Origin recognition complex 56 kDa subunit
    Gene namesi
    Name:ORC4
    Ordered Locus Names:YPR162C
    ORF Names:P9325.5
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPR162c.
    SGDiS000006366. ORC4.

    Subcellular locationi

    GO - Cellular componenti

    1. DNA replication preinitiation complex Source: SGD
    2. nuclear origin of replication recognition complex Source: SGD
    3. nuclear pre-replicative complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 529529Origin recognition complex subunit 4PRO_0000127091Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei9 – 91Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP54791.
    PaxDbiP54791.

    Expressioni

    Gene expression databases

    GenevestigatoriP54791.

    Interactioni

    Subunit structurei

    Component of the origin recognition complex (ORC) composed of at least ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ORC1P547844EBI-12580,EBI-12568
    ORC5P508745EBI-12580,EBI-12584

    Protein-protein interaction databases

    BioGridi36335. 21 interactions.
    DIPiDIP-2287N.
    IntActiP54791. 15 interactions.
    MINTiMINT-669611.
    STRINGi4932.YPR162C.

    Structurei

    3D structure databases

    ProteinModelPortaliP54791.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ORC4 family.Curated

    Phylogenomic databases

    eggNOGiNOG289784.
    GeneTreeiENSGT00390000016542.
    HOGENOMiHOG000001008.
    KOiK02606.
    OMAiCTTKLNI.
    OrthoDBiEOG70610P.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR016527. ORC4.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PIRSFiPIRSF007858. ORC4. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P54791-1 [UniParc]FASTAAdd to Basket

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    MTISEARLSP QVNLLPIKRH SNEEVEETAA ILKKRTIDNE KCKDSDPGFG    50
    SLQRRLLQQL YGTLPTDEKI IFTYLQDCQQ EIDRIIKQSI IQKESHSVIL 100
    VGPRQSYKTY LLDYELSLLQ QSYKEQFITI RLNGFIHSEQ TAINGIATQL 150
    EQQLQKIHGS EEKIDDTSLE TISSGSLTEV FEKILLLLDS TTKTRNEDSG 200
    EVDRESITKI TVVFIFDEID TFAGPVRQTL LYNLFDMVEH SRVPVCIFGC 250
    TTKLNILEYL EKRVKSRFSQ RVIYMPQIQN LDDMVDAVRN LLTVRSEISP 300
    WVSQWNETLE KELSDPRSNL NRHIRMNFET FRSLPTLKNS IIPLVATSKN 350
    FGSLCTAIKS CSFLDIYNKN QLSNNLTGRL QSLSDLELAI LISAARVALR 400
    AKDGSFNFNL AYAEYEKMIK AINSRIPTVA PTTNVGTGQS TFSIDNTIKL 450
    WLKKDVKNVW ENLVQLDFFT EKSAVGLRDN ATAAFYASNY QFQGTMIPFD 500
    LRSYQMQIIL QELRRIIPKS NMYYSWTQL 529
    Length:529
    Mass (Da):60,705
    Last modified:October 1, 1996 - v1
    Checksum:iACF064F2C633F13F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U34862 Genomic DNA. Translation: AAB38250.1.
    U25840 Genomic DNA. Translation: AAB68149.1.
    AY723871 Genomic DNA. Translation: AAU09788.1.
    BK006949 Genomic DNA. Translation: DAA11579.1.
    PIRiS59821.
    RefSeqiNP_015488.1. NM_001184259.1.

    Genome annotation databases

    EnsemblFungiiYPR162C; YPR162C; YPR162C.
    GeneIDi856291.
    KEGGisce:YPR162C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U34862 Genomic DNA. Translation: AAB38250.1 .
    U25840 Genomic DNA. Translation: AAB68149.1 .
    AY723871 Genomic DNA. Translation: AAU09788.1 .
    BK006949 Genomic DNA. Translation: DAA11579.1 .
    PIRi S59821.
    RefSeqi NP_015488.1. NM_001184259.1.

    3D structure databases

    ProteinModelPortali P54791.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36335. 21 interactions.
    DIPi DIP-2287N.
    IntActi P54791. 15 interactions.
    MINTi MINT-669611.
    STRINGi 4932.YPR162C.

    Proteomic databases

    MaxQBi P54791.
    PaxDbi P54791.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPR162C ; YPR162C ; YPR162C .
    GeneIDi 856291.
    KEGGi sce:YPR162C.

    Organism-specific databases

    CYGDi YPR162c.
    SGDi S000006366. ORC4.

    Phylogenomic databases

    eggNOGi NOG289784.
    GeneTreei ENSGT00390000016542.
    HOGENOMi HOG000001008.
    KOi K02606.
    OMAi CTTKLNI.
    OrthoDBi EOG70610P.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34291-MONOMER.

    Miscellaneous databases

    NextBioi 981632.
    PROi P54791.

    Gene expression databases

    Genevestigatori P54791.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR016527. ORC4.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PIRSFi PIRSF007858. ORC4. 1 hit.
    SUPFAMi SSF52540. SSF52540. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The multidomain structure of Orc1p reveals similarity to regulators of DNA replication and transcriptional silencing."
      Bell S.P., Mitchell J., Leber J., Kobayashi R., Stillman B.
      Cell 83:563-568(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiORC4_YEAST
    AccessioniPrimary (citable) accession number: P54791
    Secondary accession number(s): D6W4G3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2170 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3