Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Origin recognition complex subunit 1

Gene

orc1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the origin recognition complex (ORC) that binds origins of replication. It has a role in both chromosomal replication and mating type transcriptional silencing. ORC binds to multiple sites within the ars1 origin of DNA replication in an ATP-independent manner.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi368 – 3758ATPSequence analysis

GO - Molecular functioni

  • ATPase activity Source: UniProtKB
  • ATP binding Source: UniProtKB
  • chromatin binding Source: InterPro
  • DNA replication origin binding Source: UniProtKB

GO - Biological processi

  • mitotic DNA replication Source: PomBase
  • mitotic DNA replication checkpoint Source: PomBase
Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-113510. E2F mediated regulation of DNA replication.
R-SPO-68616. Assembly of the ORC complex at the origin of replication.
R-SPO-68949. Orc1 removal from chromatin.
R-SPO-68962. Activation of the pre-replicative complex.
R-SPO-69205. G1/S-Specific Transcription.
R-SPO-69298. Association of licensing factors with the pre-replicative complex.

Names & Taxonomyi

Protein namesi
Recommended name:
Origin recognition complex subunit 1
Gene namesi
Name:orc1
Synonyms:orp1
ORF Names:SPBC29A10.15
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC29A10.15.
PomBaseiSPBC29A10.15. orc1.

Subcellular locationi

GO - Cellular componenti

  • DNA replication preinitiation complex Source: PomBase
  • nuclear chromatin Source: PomBase
  • nuclear origin of replication recognition complex Source: UniProtKB
  • nuclear pre-replicative complex Source: PomBase
  • nuclear replication fork Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 707707Origin recognition complex subunit 1PRO_0000127073Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei291 – 2911Phosphoserine1 Publication
Modified residuei292 – 2921Phosphothreonine1 Publication
Modified residuei320 – 3201Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP54789.

PTM databases

iPTMnetiP54789.

Expressioni

Developmental stagei

Expressed constitutively during the cell cycle.

Interactioni

Subunit structurei

ORC is composed of six subunits. ORC interacts with cdc18, recruiting it to the ars1 origin of replication.2 Publications

Protein-protein interaction databases

BioGridi276998. 37 interactions.
MINTiMINT-4690956.

Structurei

3D structure databases

ProteinModelPortaliP54789.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini62 – 193132BAHPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ORC1 family.Curated
Contains 1 BAH domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000207574.
InParanoidiP54789.
KOiK02603.
OrthoDBiEOG7ZWD9N.
PhylomeDBiP54789.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR001025. BAH_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF01426. BAH. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00439. BAH. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51038. BAH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54789-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRRKSLRSQ LLINGIDKSL LSDDSADSSD IDEEEVYGVW TEEPFQKEAG
60 70 80 90 100
RSYYRSLKKN DVIYRVGDDI TVHDGDSSFY LGVICKLYEK AIDKHSGKKY
110 120 130 140 150
VEAIWYSRAY AKRMEIKPEY LLPDRHINEV YVSCGRDENL TSCIIEHCNV
160 170 180 190 200
YSEAEFFSKF PAGIPTKRKD LFPCNFFIRR GVHLKVNKYT EPLDWSYYAH
210 220 230 240 250
NLERIEDLLV EMEENLRPTK KKSGSRGRGR PRKYPLPNVE SKESSSKVNS
260 270 280 290 300
KDENFDLQDD SESSEDNLTI QPQTPRRRHK RSRHNSSNLA STPKRNGYKQ
310 320 330 340 350
PLQITPLPIR MLSLEEFQGS PHRKARAMLH VASVPSTLQC RDNEFSTIFS
360 370 380 390 400
NLESAIEEET GACLYISGTP GTGKTATVHE VIWNLQELSR EGQLPEFSFC
410 420 430 440 450
EINGMRVTSA NQAYSILWES LTGERVTPIH AMDLLDNRFT HASPNRSSCV
460 470 480 490 500
VLMDELDQLV THNQKVLYNF FNWPSLPHSR LIVVAVANTM DLPERILSNR
510 520 530 540 550
ISSRLGLSRV PFEPYTHTQL EIIIAARLEA VRDDDVFSSD AIRFAARKVA
560 570 580 590 600
AVSGDARRAL DICRRASELA ENKNGKVTPG LIHQAISEMT ASPLQKVLRN
610 620 630 640 650
LSFMQKVFLC AIVNRMRRSG FAESYVYEVL EEAERLLRVM TTPDAEAKFG
660 670 680 690 700
ELILRRPEFG YVLSSLSENG VLYLENKSSR NARVRLAIAD DEIKLAFRGD

SELAGIA
Length:707
Mass (Da):80,514
Last modified:October 1, 1996 - v1
Checksum:iD056018159A40A44
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40378 mRNA. Translation: AAC49141.1.
U43392 Genomic DNA. Translation: AAB38247.1.
U38522 Genomic DNA. Translation: AAC49129.1.
CU329671 Genomic DNA. Translation: CAA22443.1.
PIRiT40070.
RefSeqiNP_596060.1. NM_001021971.2.

Genome annotation databases

EnsemblFungiiSPBC29A10.15.1; SPBC29A10.15.1:pep; SPBC29A10.15.
GeneIDi2540470.
KEGGispo:SPBC29A10.15.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40378 mRNA. Translation: AAC49141.1.
U43392 Genomic DNA. Translation: AAB38247.1.
U38522 Genomic DNA. Translation: AAC49129.1.
CU329671 Genomic DNA. Translation: CAA22443.1.
PIRiT40070.
RefSeqiNP_596060.1. NM_001021971.2.

3D structure databases

ProteinModelPortaliP54789.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276998. 37 interactions.
MINTiMINT-4690956.

PTM databases

iPTMnetiP54789.

Proteomic databases

MaxQBiP54789.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC29A10.15.1; SPBC29A10.15.1:pep; SPBC29A10.15.
GeneIDi2540470.
KEGGispo:SPBC29A10.15.

Organism-specific databases

EuPathDBiFungiDB:SPBC29A10.15.
PomBaseiSPBC29A10.15. orc1.

Phylogenomic databases

HOGENOMiHOG000207574.
InParanoidiP54789.
KOiK02603.
OrthoDBiEOG7ZWD9N.
PhylomeDBiP54789.

Enzyme and pathway databases

ReactomeiR-SPO-113510. E2F mediated regulation of DNA replication.
R-SPO-68616. Assembly of the ORC complex at the origin of replication.
R-SPO-68949. Orc1 removal from chromatin.
R-SPO-68962. Activation of the pre-replicative complex.
R-SPO-69205. G1/S-Specific Transcription.
R-SPO-69298. Association of licensing factors with the pre-replicative complex.

Miscellaneous databases

NextBioi20801597.
PROiP54789.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR001025. BAH_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF01426. BAH. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00439. BAH. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51038. BAH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Conserved initiator proteins in eukaryotes."
    Gavin K.A., Hidaka M., Stillman B.D.
    Science 270:1667-1671(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Orp1, a member of the Cdc18/Cdc6 family of S-phase regulators, is homologous to a component of the origin recognition complex."
    Muzi-Falconi M., Kelly T.J.
    Proc. Natl. Acad. Sci. U.S.A. 92:12475-12479(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  4. "Identification and reconstitution of the origin recognition complex from Schizosaccharomyces pombe."
    Moon K.-Y., Kong D., Lee J.-K., Raychaudhuri S., Hurwitz J.
    Proc. Natl. Acad. Sci. U.S.A. 96:12367-12372(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  5. "Purification and characterization of the Schizosaccharomyces pombe origin recognition complex: interaction with origin DNA and Cdc18 protein."
    Chuang R.-Y., Chretien L., Dai J., Kelly T.J.
    J. Biol. Chem. 277:16920-16927(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION OF ORC, INTERACTION WITH CDC18.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-291; THR-292 AND SER-320, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiORC1_SCHPO
AccessioniPrimary (citable) accession number: P54789
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 11, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.