Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Vacuolar protein sorting-associated protein 9

Gene

VPS9

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for vacuolar protein sorting; may be required for the consumption of transport vesicles containing vacuolar protein precursors. May bind a Rab GTPase such as VPS21.

Miscellaneous

Present with 4280 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • guanyl-nucleotide exchange factor activity Source: SGD
  • ubiquitin binding Source: SGD

GO - Biological processi

  • Golgi to endosome transport Source: SGD
  • late endosome to vacuole transport via multivesicular body sorting pathway Source: SGD
  • protein localization to endosome Source: SGD
  • protein targeting to vacuole Source: SGD
  • vacuole inheritance Source: SGD

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-32682-MONOMER
ReactomeiR-SCE-8854214 TBC/RABGAPs
R-SCE-8876198 RAB GEFs exchange GTP for GDP on RABs

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 9
Alternative name(s):
Vacuolar protein-targeting protein 9
Gene namesi
Name:VPS9
Synonyms:VPT9
Ordered Locus Names:YML097C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML097C
SGDiS000004563 VPS9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001913241 – 451Vacuolar protein sorting-associated protein 9Add BLAST451

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei375PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP54787
PaxDbiP54787
PRIDEiP54787

PTM databases

iPTMnetiP54787

Interactioni

Subunit structurei

Homodimer.2 Publications

GO - Molecular functioni

  • guanyl-nucleotide exchange factor activity Source: SGD
  • ubiquitin binding Source: SGD

Protein-protein interaction databases

BioGridi35046, 577 interactors
DIPiDIP-4526N
IntActiP54787, 5 interactors
MINTiP54787
STRINGi4932.YML097C

Structurei

Secondary structure

1451
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi401 – 419Combined sources19
Helixi425 – 434Combined sources10
Helixi441 – 443Combined sources3
Helixi445 – 448Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MN3X-ray2.30A398-451[»]
1P3QX-ray1.70Q/R398-451[»]
ProteinModelPortaliP54787
SMRiP54787
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP54787

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini170 – 312VPS9PROSITE-ProRule annotationAdd BLAST143
Domaini408 – 451CUEPROSITE-ProRule annotationAdd BLAST44

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi229 – 237GBH motif I9
Motifi249 – 257GBH motif II9
Motifi287 – 295GBH motif III9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi332 – 341Glu/Lys-rich10

Domaini

The CUE domain (Coupling of ubiquitin conjugation to ER degradation) is monoubiquitin-binding and is required for intramolecular ubiquitination.

Phylogenomic databases

GeneTreeiENSGT00530000063341
HOGENOMiHOG000141911
InParanoidiP54787
KOiK20131
OMAiEDVCIAK
OrthoDBiEOG092C4H40

Family and domain databases

Gene3Di1.20.1050.80, 1 hit
InterProiView protein in InterPro
IPR003892 CUE
IPR009060 UBA-like_sf
IPR003123 VPS9
IPR037191 VPS9_dom_sf
PfamiView protein in Pfam
PF02845 CUE, 1 hit
PF02204 VPS9, 1 hit
SMARTiView protein in SMART
SM00546 CUE, 1 hit
SM00167 VPS9, 1 hit
SUPFAMiSSF109993 SSF109993, 1 hit
SSF46934 SSF46934, 1 hit
PROSITEiView protein in PROSITE
PS51140 CUE, 1 hit
PS51205 VPS9, 1 hit

Sequencei

Sequence statusi: Complete.

P54787-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDDEKREIL KEFDPFSQLE QANGNPDKDV KFKKDDPNRA AAEETNRDIS
60 70 80 90 100
AQDKGDEEPF YDFQIFIKQL QTPGADPLVK YTKSFLRNFL AQRLLWTVSE
110 120 130 140 150
EIKLISDFKT FIYDKFTLYE PFRSLDNSKM RNAKEGMEKL IMGKLYSRCF
160 170 180 190 200
SPSLYEILQK PLDDEHMKDL TNDDTLLEKI RHYRFISPIM LDIPDTMPNA
210 220 230 240 250
RLNKFVHLAS KELGKINRFK SPRDKMVCVL NASKVIFGLL KHTKLEQNGA
260 270 280 290 300
DSFIPVLIYC ILKGQVRYLV SNVNYIERFR SPDFIRGEEE YYLSSLQAAL
310 320 330 340 350
NFIMNLTERS LTIEDHEDFE EAYQRNFKQL AEEKEEEEKK KQLEIPDELQ
360 370 380 390 400
PNGTLLKPLD EVTNIVISKF NELFSPIGEP TQEEALKSEQ SNKEEDVSSL
410 420 430 440 450
IKKIEENERK DTLNTLQNMF PDMDPSLIED VCIAKKSRIG PCVDALLSLS

E
Length:451
Mass (Da):52,483
Last modified:October 5, 2010 - v2
Checksum:i6631766C00D3A22B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti172N → T in AAA62233 (Ref. 2) Curated1
Sequence conflicti305N → S in AAC49314 (PubMed:8628304).Curated1
Sequence conflicti305N → S in AAA62233 (Ref. 2) Curated1
Sequence conflicti314E → D in AAC49314 (PubMed:8628304).Curated1
Sequence conflicti314E → D in AAA62233 (Ref. 2) Curated1
Sequence conflicti327F → L in AAC49314 (PubMed:8628304).Curated1
Sequence conflicti327F → L in AAA62233 (Ref. 2) Curated1
Sequence conflicti345I → V in AAC49314 (PubMed:8628304).Curated1
Sequence conflicti345I → V in AAA62233 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U50142 Genomic DNA Translation: AAC49314.1
U20373 Genomic DNA Translation: AAA62233.1
Z46660 Genomic DNA Translation: CAA86640.1
BK006946 Genomic DNA Translation: DAA09802.1
PIRiS49629
RefSeqiNP_013612.1, NM_001182457.1

Genome annotation databases

EnsemblFungiiYML097C; YML097C; YML097C
GeneIDi854876
KEGGisce:YML097C

Similar proteinsi

Entry informationi

Entry nameiVPS9_YEAST
AccessioniPrimary (citable) accession number: P54787
Secondary accession number(s): D6W0I8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 5, 2010
Last modified: May 23, 2018
This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health