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P54786 (ZDS2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein ZDS2
Gene names
Name:ZDS2
Synonyms:MCS1
Ordered Locus Names:YML109W
ORF Names:YM8339.10
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length942 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a negative regulator of polarized growth via an alternative mechanism to ZDS1. In heat-stressed cells appears to play a role in localizing BCY1 to the cytoplasm. Seems to interact with, and down-regulate, CDC42. Also acts as a suppressor of PKC1. May act as an integration point for distinct signaling pathways helping to maintain a balance among these different pathways. Ref.5 Ref.7

Subunit structure

Interacts with SKG6. Ref.4

Miscellaneous

'ZDS' means 'zillion different screens' as both ZDS1 and ZDS2 have been found by a wide variety of genetic screens.

Present with 105 molecules/cell in log phase SD medium.

Sequence similarities

To yeast ZDS1/NRC1/CES1.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 942942Protein ZDS2
PRO_0000066570

Amino acid modifications

Modified residue501Phosphoserine Ref.8

Experimental info

Sequence conflict71M → V in AAB37541. Ref.1
Sequence conflict231T → S in AAB37541. Ref.1
Sequence conflict5301A → P in AAB37541. Ref.1
Sequence conflict5461A → G in AAB37541. Ref.1
Sequence conflict6331V → A in AAB37541. Ref.1
Sequence conflict6681N → S in AAB37541. Ref.1
Sequence conflict7111V → VNC in AAB37541. Ref.1
Sequence conflict7231Missing in AAB37541. Ref.1
Sequence conflict7291A → E in AAB37541. Ref.1
Sequence conflict8331S → P in AAB37541. Ref.1
Sequence conflict914 – 94229TGDIA…LIPDI → HWRYSLQW in AAB37541. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P54786 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: A1C9DD9A539E4291

FASTA942105,496
        10         20         30         40         50         60 
MVLMEDMQNK DGHNTVENSS GGTDSNNNIQ MRRMRKTQLS KKELFEKRKS DVLIAAKSLD 

        70         80         90        100        110        120 
TEIQNVKNLK RLSIGSMDLV IDPELEFKVN SRNSYSSDSS KESLQESLHE ENIIRSEQKE 

       130        140        150        160        170        180 
EQGSEDNDAY EEGDATNVDD SIDITQTEYL HDEETLEKEK IIRNASSSTS SSARVTSRNR 

       190        200        210        220        230        240 
RLSGVKTLAH DVVLDVENDH DSKMVDLTQN LLWVPADQHP NVKPENYLEL IQDTLQNIQI 

       250        260        270        280        290        300 
STNQDIDENK LELGNNHVIS NRKRTGSVVR RPSRLKTSYT KFDDEPPLAD KPQEGEIQVD 

       310        320        330        340        350        360 
KRISSSDIKT IRSVSLKEIT EELTKISNNA GLTDSDAVTL ARSLSMSGSF TNESLHLNGN 

       370        380        390        400        410        420 
HTENDNEFAS NMFNETGLTI PERSSLRRSK FNTYKIRLEG SSLPQAVKLN SLMNIQTNDN 

       430        440        450        460        470        480 
RRSASSPASY TQVPQEQASL NDFHEIFDHY RRTSTDWSTE NEKYVDSTNY YSDEEDLTHA 

       490        500        510        520        530        540 
SISQESSLLS TDSSNNSVLI KPHNTGSMIS EKLDQHVSSS EKSNTNNSEA NHGWSWLNSS 

       550        560        570        580        590        600 
NGSLNANEQT YQQLTDDEDD EECVDNEKAD FVNLSVSRRA KSTKRASERI NHSKNRHSPI 

       610        620        630        640        650        660 
FQIHSEEAKS VVITPSVVSS SESQPSKPTA PAVVEKKVEL PTDTQASTHK KNSLEKRLAK 

       670        680        690        700        710        720 
LFKRKQHNGT CKSDVKVIKK SVKKELKKKA SHSSLSKFRK SPKKKPQEAE VERPSSPTKT 

       730        740        750        760        770        780 
ITTEDIDTAS VIEPEVRSSN ASTLLPDSHT SHSSEFVVET ISELDGDDSF DISGGDVNYD 

       790        800        810        820        830        840 
VEVHSSISRD TTAGLEEDIG AEREDNTSPT APQISTLPPR KLTFEDVVKP DYSNAPIKFT 

       850        860        870        880        890        900 
DSAFGFPLPM ITNSTVIMFD HRLGINVERA IYRLSHLKLS DPGRELRQQV LLSNFMYSYL 

       910        920        930        940 
NLVNHTLYME QVGTGDIAFN GDSALGMMDK NDSDGTILIP DI 

« Hide

References

« Hide 'large scale' references
[1]"Mutations in the homologous ZDS1 and ZDS2 genes affect cell cycle progression."
Yu Y., Jiang Y.W., Wellinger R.J., Carlson K., Roberts J.M., Stillman D.J.
Mol. Cell. Biol. 16:5254-5263(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"A protein interaction map for cell polarity development."
Drees B.L., Sundin B.A., Brazeau E., Caviston J.P., Chen G.-C., Guo W., Kozminski K.G., Lau M.W., Moskow J.J., Tong A., Schenkman L.R., McKenzie A. III, Brennwald P.J., Longtine M., Bi E., Chan C., Novick P., Boone C. expand/collapse author list , Pringle J.R., Davis T.N., Fields S., Drubin D.G.
J. Cell Biol. 154:549-571(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SKG6.
[5]"Feedback inhibition on cell wall integrity signaling by Zds1 involves Gsk3 phosphorylation of a cAMP-dependent protein kinase regulatory subunit."
Griffioen G., Swinnen S., Thevelein J.M.
J. Biol. Chem. 278:23460-23471(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Pkc1 acts through Zds1 and Gic1 to suppress growth and cell polarity defects of a yeast eIF5A mutant."
Zanelli C.F., Valentini S.R.
Genetics 171:1571-1581(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[9]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U32938 Genomic DNA. Translation: AAB37541.1.
Z49210 Genomic DNA. Translation: CAA89109.1.
BK006946 Genomic DNA. Translation: DAA09789.1.
PIRS53963.
RefSeqNP_013598.1. NM_001182471.1.

3D structure databases

ProteinModelPortalP54786.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35095. 76 interactions.
DIPDIP-1517N.
IntActP54786. 56 interactions.
MINTMINT-411150.

Proteomic databases

MaxQBP54786.
PaxDbP54786.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYML109W; YML109W; YML109W.
GeneID854931.
KEGGsce:YML109W.

Organism-specific databases

CYGDYML109w.
SGDS000004577. ZDS2.

Phylogenomic databases

eggNOGNOG68442.
GeneTreeENSGT00530000067622.
HOGENOMHOG000144432.
OMARRSKFNT.
OrthoDBEOG7BW0SX.

Enzyme and pathway databases

BioCycYEAST:G3O-32692-MONOMER.

Gene expression databases

GenevestigatorP54786.

Family and domain databases

InterProIPR013941. ZDS1_C.
[Graphical view]
PfamPF08632. Zds_C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio977962.

Entry information

Entry nameZDS2_YEAST
AccessionPrimary (citable) accession number: P54786
Secondary accession number(s): D6W0H5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD