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P54786

- ZDS2_YEAST

UniProt

P54786 - ZDS2_YEAST

Protein

Protein ZDS2

Gene

ZDS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Acts as a negative regulator of polarized growth via an alternative mechanism to ZDS1. In heat-stressed cells appears to play a role in localizing BCY1 to the cytoplasm. Seems to interact with, and down-regulate, CDC42. Also acts as a suppressor of PKC1. May act as an integration point for distinct signaling pathways helping to maintain a balance among these different pathways.2 Publications

    GO - Molecular functioni

    1. identical protein binding Source: IntAct
    2. protein binding Source: IntAct

    GO - Biological processi

    1. cell aging Source: SGD
    2. chromatin silencing at rDNA Source: SGD
    3. cytokinesis after mitosis checkpoint Source: SGD
    4. establishment of cell polarity Source: SGD
    5. positive regulation of G2/M transition of mitotic cell cycle Source: SGD
    6. regulation of protein localization Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32692-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein ZDS2
    Gene namesi
    Name:ZDS2
    Synonyms:MCS1
    Ordered Locus Names:YML109W
    ORF Names:YM8339.10
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYML109w.
    SGDiS000004577. ZDS2.

    Subcellular locationi

    GO - Cellular componenti

    1. cellular bud neck Source: SGD
    2. cellular bud tip Source: SGD
    3. cytoplasm Source: SGD
    4. nucleus Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 942942Protein ZDS2PRO_0000066570Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei50 – 501Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP54786.
    PaxDbiP54786.

    Expressioni

    Gene expression databases

    GenevestigatoriP54786.

    Interactioni

    Subunit structurei

    Interacts with SKG6.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself3EBI-29637,EBI-29637
    BOI1P380413EBI-29637,EBI-3719
    CDC55Q003629EBI-29637,EBI-1942
    SLA1P327903EBI-29637,EBI-17313

    Protein-protein interaction databases

    BioGridi35095. 76 interactions.
    DIPiDIP-1517N.
    IntActiP54786. 56 interactions.
    MINTiMINT-411150.

    Structurei

    3D structure databases

    ProteinModelPortaliP54786.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    To yeast ZDS1/NRC1/CES1.Curated

    Phylogenomic databases

    eggNOGiNOG68442.
    GeneTreeiENSGT00530000067622.
    HOGENOMiHOG000144432.
    OMAiRRSKFNT.
    OrthoDBiEOG7BW0SX.

    Family and domain databases

    InterProiIPR013941. ZDS1_C.
    [Graphical view]
    PfamiPF08632. Zds_C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P54786-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVLMEDMQNK DGHNTVENSS GGTDSNNNIQ MRRMRKTQLS KKELFEKRKS    50
    DVLIAAKSLD TEIQNVKNLK RLSIGSMDLV IDPELEFKVN SRNSYSSDSS 100
    KESLQESLHE ENIIRSEQKE EQGSEDNDAY EEGDATNVDD SIDITQTEYL 150
    HDEETLEKEK IIRNASSSTS SSARVTSRNR RLSGVKTLAH DVVLDVENDH 200
    DSKMVDLTQN LLWVPADQHP NVKPENYLEL IQDTLQNIQI STNQDIDENK 250
    LELGNNHVIS NRKRTGSVVR RPSRLKTSYT KFDDEPPLAD KPQEGEIQVD 300
    KRISSSDIKT IRSVSLKEIT EELTKISNNA GLTDSDAVTL ARSLSMSGSF 350
    TNESLHLNGN HTENDNEFAS NMFNETGLTI PERSSLRRSK FNTYKIRLEG 400
    SSLPQAVKLN SLMNIQTNDN RRSASSPASY TQVPQEQASL NDFHEIFDHY 450
    RRTSTDWSTE NEKYVDSTNY YSDEEDLTHA SISQESSLLS TDSSNNSVLI 500
    KPHNTGSMIS EKLDQHVSSS EKSNTNNSEA NHGWSWLNSS NGSLNANEQT 550
    YQQLTDDEDD EECVDNEKAD FVNLSVSRRA KSTKRASERI NHSKNRHSPI 600
    FQIHSEEAKS VVITPSVVSS SESQPSKPTA PAVVEKKVEL PTDTQASTHK 650
    KNSLEKRLAK LFKRKQHNGT CKSDVKVIKK SVKKELKKKA SHSSLSKFRK 700
    SPKKKPQEAE VERPSSPTKT ITTEDIDTAS VIEPEVRSSN ASTLLPDSHT 750
    SHSSEFVVET ISELDGDDSF DISGGDVNYD VEVHSSISRD TTAGLEEDIG 800
    AEREDNTSPT APQISTLPPR KLTFEDVVKP DYSNAPIKFT DSAFGFPLPM 850
    ITNSTVIMFD HRLGINVERA IYRLSHLKLS DPGRELRQQV LLSNFMYSYL 900
    NLVNHTLYME QVGTGDIAFN GDSALGMMDK NDSDGTILIP DI 942
    Length:942
    Mass (Da):105,496
    Last modified:October 1, 1996 - v1
    Checksum:iA1C9DD9A539E4291
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti7 – 71M → V in AAB37541. (PubMed:8816438)Curated
    Sequence conflicti23 – 231T → S in AAB37541. (PubMed:8816438)Curated
    Sequence conflicti530 – 5301A → P in AAB37541. (PubMed:8816438)Curated
    Sequence conflicti546 – 5461A → G in AAB37541. (PubMed:8816438)Curated
    Sequence conflicti633 – 6331V → A in AAB37541. (PubMed:8816438)Curated
    Sequence conflicti668 – 6681N → S in AAB37541. (PubMed:8816438)Curated
    Sequence conflicti711 – 7111V → VNC in AAB37541. (PubMed:8816438)Curated
    Sequence conflicti723 – 7231Missing in AAB37541. (PubMed:8816438)Curated
    Sequence conflicti729 – 7291A → E in AAB37541. (PubMed:8816438)Curated
    Sequence conflicti833 – 8331S → P in AAB37541. (PubMed:8816438)Curated
    Sequence conflicti914 – 94229TGDIA…LIPDI → HWRYSLQW in AAB37541. (PubMed:8816438)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U32938 Genomic DNA. Translation: AAB37541.1.
    Z49210 Genomic DNA. Translation: CAA89109.1.
    BK006946 Genomic DNA. Translation: DAA09789.1.
    PIRiS53963.
    RefSeqiNP_013598.1. NM_001182471.1.

    Genome annotation databases

    EnsemblFungiiYML109W; YML109W; YML109W.
    GeneIDi854931.
    KEGGisce:YML109W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U32938 Genomic DNA. Translation: AAB37541.1 .
    Z49210 Genomic DNA. Translation: CAA89109.1 .
    BK006946 Genomic DNA. Translation: DAA09789.1 .
    PIRi S53963.
    RefSeqi NP_013598.1. NM_001182471.1.

    3D structure databases

    ProteinModelPortali P54786.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35095. 76 interactions.
    DIPi DIP-1517N.
    IntActi P54786. 56 interactions.
    MINTi MINT-411150.

    Proteomic databases

    MaxQBi P54786.
    PaxDbi P54786.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YML109W ; YML109W ; YML109W .
    GeneIDi 854931.
    KEGGi sce:YML109W.

    Organism-specific databases

    CYGDi YML109w.
    SGDi S000004577. ZDS2.

    Phylogenomic databases

    eggNOGi NOG68442.
    GeneTreei ENSGT00530000067622.
    HOGENOMi HOG000144432.
    OMAi RRSKFNT.
    OrthoDBi EOG7BW0SX.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32692-MONOMER.

    Miscellaneous databases

    NextBioi 977962.

    Gene expression databases

    Genevestigatori P54786.

    Family and domain databases

    InterProi IPR013941. ZDS1_C.
    [Graphical view ]
    Pfami PF08632. Zds_C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mutations in the homologous ZDS1 and ZDS2 genes affect cell cycle progression."
      Yu Y., Jiang Y.W., Wellinger R.J., Carlson K., Roberts J.M., Stillman D.J.
      Mol. Cell. Biol. 16:5254-5263(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: INTERACTION WITH SKG6.
    5. "Feedback inhibition on cell wall integrity signaling by Zds1 involves Gsk3 phosphorylation of a cAMP-dependent protein kinase regulatory subunit."
      Griffioen G., Swinnen S., Thevelein J.M.
      J. Biol. Chem. 278:23460-23471(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Pkc1 acts through Zds1 and Gic1 to suppress growth and cell polarity defects of a yeast eIF5A mutant."
      Zanelli C.F., Valentini S.R.
      Genetics 171:1571-1581(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    9. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiZDS2_YEAST
    AccessioniPrimary (citable) accession number: P54786
    Secondary accession number(s): D6W0H5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    'ZDS' means 'zillion different screens' as both ZDS1 and ZDS2 have been found by a wide variety of genetic screens.
    Present with 105 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3