P54785 (MOT3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcriptional activator/repressor MOT3

Alternative name(s):
Hypoxic gene repressor protein 7
Modulator of transcription protein 3

Gene names

Name:	MOT3
Synonyms:	ROX7
Ordered Locus Names:	YMR070W
ORF Names:	YM9916.09

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription factor that affects the expression of a large set of genes. Recognizes and binds to the consensus sequence 5'-[CAT]AGG[TC]A-3' in the promoter region. Plays a major role in the repression of a specific subset of hypoxic genes (e.g. ANB1, DAN1 and HEM13) under aerobic conditions. Acts synergistically with the transcription factor ROX1 to recruit the general repression complex SSN6-TUP1 to the promoter of hypoxic genes. Represses transcription of ergosterol biosynthetic genes. Negatively regulates pheromone-induced gene expression. Can act as a transcriptional activator (e.g. of genes like CYC1, SUC2 and the Ty long terminal repeat delta promoter). Ref.1 Ref.5 Ref.6 Ref.7 Ref.9 Ref.10
Subcellular location	Nucleus Ref.5.
Induction	Induced under aerobic conditions and repressed under anaerobic conditions. Ref.9
Miscellaneous	Present with 1690 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Contains 2 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Activator Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype Ref.6Ref.7. Source: SGD transcription, DNA-dependent Inferred from genetic interaction. Source: SGD
Cellular component	nucleus Inferred from direct assay Ref.5. Source: SGD
Molecular function	DNA binding Inferred from physical interaction. Source: SGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 490	490	Transcriptional activator/repressor MOT3		PRO_0000046807
Regions
	Zinc finger	346 – 368	23	C2H2-type 1
	Zinc finger	374 – 397	24	C2H2-type 2
	Compositional bias	8 – 35	28	Poly-Gln
	Compositional bias	98 – 104	7	Poly-Asn
	Compositional bias	143 – 157	15	Poly-Asn
	Compositional bias	173 – 177	5	Poly-Ala
	Compositional bias	240 – 245	6	Poly-His
	Compositional bias	417 – 420	4	Poly-Ser
	Compositional bias	421 – 433	13	Poly-Asn
	Compositional bias	441 – 450	10	Poly-Ala

Sequences

	Sequence		Length	Mass (Da)
	P54785 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 4D0DA8DE43F171ED	FASTA	490	54,382
	10 20 30 40 50 60 MNADHHLQQQ QQQRQQHQQQ QHQQQQHQHQ HQQQQHTILQ NVSNTNNIGS DSLASQPFNT 70 80 90 100 110 120 TTVSSNKDDV MVNSGARELP MPLHQQQYIY PYYQYTSNNS NNNNVTAGNN MSASPIVHNN 130 140 150 160 170 180 SNNSNNSNIS ASDYTVANNS TSNNNNNNNN NNNNNNNIHP NQFTAAANMN SNAAAAAYYS 190 200 210 220 230 240 FPTANMPIPQ QDQQYMFNPA SYISHYYSAV NSNNNGNNAA NNGSNNSSHS APAPAPGPPH 250 260 270 280 290 300 HHHHHSNTHN NLNNGGAVNT NNAPQHHPTI ITDQFQFQLQ QNPSPNLNLN INPAQPLHLP 310 320 330 340 350 360 PGWKINTMPQ PRPTTAPNHP PAPVPSSNPV ASNLVPAPSS DHKYIHQCQF CEKSFKRKSW 370 380 390 400 410 420 LKRHLLSHSQ QRHFLCPWCL SRQKRKDNLL QHMKLKHTNY LLDELKKNNI IFNYNNSSSS 430 440 450 460 470 480 NNNNDNNNNN NSNSASGSGG AGAAAAAATA PENEDGNGYD TNIKTLINDG VLNKDDVKRV 490 LNNLIVSHNK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and analysis of Mot3, a zinc finger protein that binds to the retrotransposon Ty long terminal repeat (delta) in Saccharomyces cerevisiae." Madison J.M., Dudley A.M., Winston F. Mol. Cell. Biol. 18:1879-1890(1998) [PubMed: 9528759] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DNA-BINDING. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Mot3, a Zn finger transcription factor that modulates gene expression and attenuates mating pheromone signaling in Saccharomyces cerevisiae." Grishin A.V., Rothenberg M., Downs M.A., Blumer K.J. Genetics 149:879-892(1998) [PubMed: 9611199] [Abstract] Cited for: FUNCTION IN TRANSCRIPTIONAL ACTIVATION, DNA-BINDING, SUBCELLULAR LOCATION.
[6]	"Roles of transcription factor Mot3 and chromatin in repression of the hypoxic gene ANB1 in yeast." Kastaniotis A.J., Mennella T.A., Konrad C., Rodriguez Torres A.M., Zitomer R.S. Mol. Cell. Biol. 20:7088-7098(2000) [PubMed: 10982825] [Abstract] Cited for: FUNCTION.
[7]	"Mot3 is a transcriptional repressor of ergosterol biosynthetic genes and is required for normal vacuolar function in Saccharomyces cerevisiae." Hongay C., Jia N., Bard M., Winston F. EMBO J. 21:4114-4124(2002) [PubMed: 12145211] [Abstract] Cited for: FUNCTION IN TRANSCRIPTIONAL REPRESSION.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Synergistic repression of anaerobic genes by Mot3 and Rox1 in Saccharomyces cerevisiae." Sertil O., Kapoor R., Cohen B.D., Abramova N., Lowry C.V. Nucleic Acids Res. 31:5831-5837(2003) [PubMed: 14530431] [Abstract] Cited for: FUNCTION IN REPRESSION OF HYPOXIC GENES, INDUCTION.
[10]	"Combinatorial repression of the hypoxic genes of Saccharomyces cerevisiae by DNA binding proteins Rox1 and Mot3." Klinkenberg L.G., Mennella T.A., Luetkenhaus K., Zitomer R.S. Eukaryot. Cell 4:649-660(2005) [PubMed: 15821125] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U25279 Genomic DNA. Translation: AAC49982.1. Z48952 Genomic DNA. Translation: CAA88795.1. AY693209 Genomic DNA. Translation: AAT93228.1. BK006946 Genomic DNA. Translation: DAA09968.1.
PIR	S52830.
RefSeq	NP_013786.1. NM_001182568.1.
3D structure databases
ProteinModelPortal	P54785.
SMR	P54785. Positions 344-429.
ModBase	Search...
Protein-protein interaction databases
IntAct	P54785. 28 interactions.
MINT	MINT-4497702.
STRING	P54785.
Proteomic databases
PeptideAtlas	P54785.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR070W; YMR070W; YMR070W.
GeneID	855092.
KEGG	sce:YMR070W.
NMPDR	fig|4932.3.peg.4833.
Organism-specific databases
CYGD	YMR070w.
SGD	S000004674. MOT3.
Phylogenomic databases
eggNOG	fuNOG11955.
GeneTree	EFGT00050000000132.
OMA	KDNLLQH.
Gene expression databases
ArrayExpress	P54785.
Genevestigator	P54785.
GermOnline	YMR070W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Gene3D	G3DSA:3.30.160.60. Znf_C2H2/integrase_DNA-bd. 2 hits.
Pfam	PF00096. zf-C2H2. 1 hit. [Graphical view]
SMART	SM00355. ZnF_C2H2. 2 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 1 hit. PS50157. ZINC_FINGER_C2H2_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978399.

Entry information

Entry name

MOT3_YEAST

Accession

Primary (citable) accession number: P54785
Secondary accession number(s): D6VZP4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program