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Protein

Transcriptional activator/repressor MOT3

Gene

MOT3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that affects the expression of a large set of genes. Recognizes and binds to the consensus sequence 5'-[CAT]AGG[TC]A-3' in the promoter region. Plays a major role in the repression of a specific subset of hypoxic genes (e.g. ANB1, DAN1 and HEM13) under aerobic conditions. Acts synergistically with the transcription factor ROX1 to recruit the general repression complex SSN6-TUP1 to the promoter of hypoxic genes. Represses transcription of ergosterol biosynthetic genes. Negatively regulates pheromone-induced gene expression. Can act as a transcriptional activator (e.g. of genes like CYC1, SUC2 and the Ty long terminal repeat delta promoter).6 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri346 – 36823C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri374 – 39724C2H2-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • RNA polymerase II activating transcription factor binding Source: SGD
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: SGD
  • RNA polymerase II repressing transcription factor binding Source: SGD
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: SGD

GO - Biological processi

  • cellular hyperosmotic response Source: SGD
  • cellular response to osmotic stress Source: SGD
  • negative regulation of ergosterol biosynthetic process Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter in response to osmotic stress Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Prion, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32772-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional activator/repressor MOT3
Alternative name(s):
Hypoxic gene repressor protein 7
Modulator of transcription protein 3
Gene namesi
Name:MOT3
Synonyms:ROX7
Ordered Locus Names:YMR070W
ORF Names:YM9916.09
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR070W.
SGDiS000004674. MOT3.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • cytosol Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Amyloid, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 490490Transcriptional activator/repressor MOT3PRO_0000046807Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP54785.

Expressioni

Inductioni

Induced under aerobic conditions and repressed under anaerobic conditions.1 Publication

Interactioni

GO - Molecular functioni

  • RNA polymerase II activating transcription factor binding Source: SGD
  • RNA polymerase II repressing transcription factor binding Source: SGD

Protein-protein interaction databases

BioGridi35245. 117 interactions.
IntActiP54785. 26 interactions.
MINTiMINT-4497702.

Structurei

3D structure databases

ProteinModelPortaliP54785.
SMRiP54785. Positions 344-399.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni98 – 295198Prion domain (PrD)Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi8 – 3528Poly-GlnAdd
BLAST
Compositional biasi98 – 1047Poly-Asn
Compositional biasi143 – 15715Poly-AsnAdd
BLAST
Compositional biasi173 – 1775Poly-Ala
Compositional biasi240 – 2456Poly-His
Compositional biasi417 – 4204Poly-Ser
Compositional biasi421 – 43313Poly-AsnAdd
BLAST
Compositional biasi441 – 45010Poly-Ala

Domaini

The prion domain (PrD) is a Gln/Asn (Q/N)-rich domain, which is unstructured in its native, soluble form, and which forms a parallel in-register beta-sheet in its amyloid form.By similarity

Sequence similaritiesi

Contains 2 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri346 – 36823C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri374 – 39724C2H2-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

InParanoidiP54785.
OMAiASAFTHN.
OrthoDBiEOG092C5U4J.

Family and domain databases

Gene3Di3.30.160.60. 2 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 2 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54785-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNADHHLQQQ QQQRQQHQQQ QHQQQQHQHQ HQQQQHTILQ NVSNTNNIGS
60 70 80 90 100
DSLASQPFNT TTVSSNKDDV MVNSGARELP MPLHQQQYIY PYYQYTSNNS
110 120 130 140 150
NNNNVTAGNN MSASPIVHNN SNNSNNSNIS ASDYTVANNS TSNNNNNNNN
160 170 180 190 200
NNNNNNNIHP NQFTAAANMN SNAAAAAYYS FPTANMPIPQ QDQQYMFNPA
210 220 230 240 250
SYISHYYSAV NSNNNGNNAA NNGSNNSSHS APAPAPGPPH HHHHHSNTHN
260 270 280 290 300
NLNNGGAVNT NNAPQHHPTI ITDQFQFQLQ QNPSPNLNLN INPAQPLHLP
310 320 330 340 350
PGWKINTMPQ PRPTTAPNHP PAPVPSSNPV ASNLVPAPSS DHKYIHQCQF
360 370 380 390 400
CEKSFKRKSW LKRHLLSHSQ QRHFLCPWCL SRQKRKDNLL QHMKLKHTNY
410 420 430 440 450
LLDELKKNNI IFNYNNSSSS NNNNDNNNNN NSNSASGSGG AGAAAAAATA
460 470 480 490
PENEDGNGYD TNIKTLINDG VLNKDDVKRV LNNLIVSHNK
Length:490
Mass (Da):54,382
Last modified:October 1, 1996 - v1
Checksum:i4D0DA8DE43F171ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25279 Genomic DNA. Translation: AAC49982.1.
Z48952 Genomic DNA. Translation: CAA88795.1.
AY693209 Genomic DNA. Translation: AAT93228.1.
BK006946 Genomic DNA. Translation: DAA09968.1.
PIRiS52830.
RefSeqiNP_013786.1. NM_001182568.1.

Genome annotation databases

EnsemblFungiiYMR070W; YMR070W; YMR070W.
GeneIDi855092.
KEGGisce:YMR070W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25279 Genomic DNA. Translation: AAC49982.1.
Z48952 Genomic DNA. Translation: CAA88795.1.
AY693209 Genomic DNA. Translation: AAT93228.1.
BK006946 Genomic DNA. Translation: DAA09968.1.
PIRiS52830.
RefSeqiNP_013786.1. NM_001182568.1.

3D structure databases

ProteinModelPortaliP54785.
SMRiP54785. Positions 344-399.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35245. 117 interactions.
IntActiP54785. 26 interactions.
MINTiMINT-4497702.

Proteomic databases

MaxQBiP54785.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR070W; YMR070W; YMR070W.
GeneIDi855092.
KEGGisce:YMR070W.

Organism-specific databases

EuPathDBiFungiDB:YMR070W.
SGDiS000004674. MOT3.

Phylogenomic databases

InParanoidiP54785.
OMAiASAFTHN.
OrthoDBiEOG092C5U4J.

Enzyme and pathway databases

BioCyciYEAST:G3O-32772-MONOMER.

Miscellaneous databases

PROiP54785.

Family and domain databases

Gene3Di3.30.160.60. 2 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 2 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMOT3_YEAST
AccessioniPrimary (citable) accession number: P54785
Secondary accession number(s): D6VZP4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

[MOT3+] is the prion form of MOT3. [MOT3+] is the result of a conformational change of the cellular MOT3 protein that becomes self-propagating and infectious. This conformational change generates a form of MOT3 that assembles into amyloid fibrils. [MOT3+]-aggregates sequester soluble MOT3, resulting in a loss-of-function phenotype for MOT3. [MOT3+] can be cured by GdnHCl and by inactivation of the molecular chaperone HSP104, which is required for [MOT3+] propagation. It is speculated that prion properties of transcription factors may generate an optimized phenotypic heterogeneity that buffers yeast populations against diverse environmental insults.1 Publication
Present with 1690 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.