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P54785

- MOT3_YEAST

UniProt

P54785 - MOT3_YEAST

Protein

Transcriptional activator/repressor MOT3

Gene

MOT3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Transcription factor that affects the expression of a large set of genes. Recognizes and binds to the consensus sequence 5'-[CAT]AGG[TC]A-3' in the promoter region. Plays a major role in the repression of a specific subset of hypoxic genes (e.g. ANB1, DAN1 and HEM13) under aerobic conditions. Acts synergistically with the transcription factor ROX1 to recruit the general repression complex SSN6-TUP1 to the promoter of hypoxic genes. Represses transcription of ergosterol biosynthetic genes. Negatively regulates pheromone-induced gene expression. Can act as a transcriptional activator (e.g. of genes like CYC1, SUC2 and the Ty long terminal repeat delta promoter).6 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri346 – 36823C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri374 – 39724C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. RNA polymerase II activating transcription factor binding Source: SGD
    3. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: SGD
    4. RNA polymerase II repressing transcription factor binding Source: SGD

    GO - Biological processi

    1. cellular hyperosmotic response Source: SGD
    2. negative regulation of ergosterol biosynthetic process Source: SGD
    3. negative regulation of transcription from RNA polymerase II promoter Source: SGD
    4. positive regulation of transcription from RNA polymerase II promoter Source: SGD
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Prion, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32772-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcriptional activator/repressor MOT3
    Alternative name(s):
    Hypoxic gene repressor protein 7
    Modulator of transcription protein 3
    Gene namesi
    Name:MOT3
    Synonyms:ROX7
    Ordered Locus Names:YMR070W
    ORF Names:YM9916.09
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR070w.
    SGDiS000004674. MOT3.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. cytosol Source: SGD
    2. nucleus Source: SGD

    Keywords - Cellular componenti

    Amyloid, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 490490Transcriptional activator/repressor MOT3PRO_0000046807Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP54785.
    PaxDbiP54785.
    PeptideAtlasiP54785.

    Expressioni

    Inductioni

    Induced under aerobic conditions and repressed under anaerobic conditions.1 Publication

    Gene expression databases

    GenevestigatoriP54785.

    Interactioni

    Protein-protein interaction databases

    BioGridi35245. 115 interactions.
    IntActiP54785. 26 interactions.
    MINTiMINT-4497702.
    STRINGi4932.YMR070W.

    Structurei

    3D structure databases

    ProteinModelPortaliP54785.
    SMRiP54785. Positions 339-432.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni98 – 295198Prion domain (PrD)Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi8 – 3528Poly-GlnAdd
    BLAST
    Compositional biasi98 – 1047Poly-Asn
    Compositional biasi143 – 15715Poly-AsnAdd
    BLAST
    Compositional biasi173 – 1775Poly-Ala
    Compositional biasi240 – 2456Poly-His
    Compositional biasi417 – 4204Poly-Ser
    Compositional biasi421 – 43313Poly-AsnAdd
    BLAST
    Compositional biasi441 – 45010Poly-Ala

    Domaini

    The prion domain (PrD) is a Gln/Asn (Q/N)-rich domain, which is unstructured in its native, soluble form, and which forms a parallel in-register beta-sheet in its amyloid form.By similarity

    Sequence similaritiesi

    Contains 2 C2H2-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri346 – 36823C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri374 – 39724C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG255610.
    OMAiASYISHY.
    OrthoDBiEOG757D9C.

    Family and domain databases

    Gene3Di3.30.160.60. 2 hits.
    InterProiIPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view]
    PfamiPF00096. zf-C2H2. 1 hit.
    [Graphical view]
    SMARTiSM00355. ZnF_C2H2. 2 hits.
    [Graphical view]
    PROSITEiPS00028. ZINC_FINGER_C2H2_1. 1 hit.
    PS50157. ZINC_FINGER_C2H2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P54785-1 [UniParc]FASTAAdd to Basket

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    MNADHHLQQQ QQQRQQHQQQ QHQQQQHQHQ HQQQQHTILQ NVSNTNNIGS    50
    DSLASQPFNT TTVSSNKDDV MVNSGARELP MPLHQQQYIY PYYQYTSNNS 100
    NNNNVTAGNN MSASPIVHNN SNNSNNSNIS ASDYTVANNS TSNNNNNNNN 150
    NNNNNNNIHP NQFTAAANMN SNAAAAAYYS FPTANMPIPQ QDQQYMFNPA 200
    SYISHYYSAV NSNNNGNNAA NNGSNNSSHS APAPAPGPPH HHHHHSNTHN 250
    NLNNGGAVNT NNAPQHHPTI ITDQFQFQLQ QNPSPNLNLN INPAQPLHLP 300
    PGWKINTMPQ PRPTTAPNHP PAPVPSSNPV ASNLVPAPSS DHKYIHQCQF 350
    CEKSFKRKSW LKRHLLSHSQ QRHFLCPWCL SRQKRKDNLL QHMKLKHTNY 400
    LLDELKKNNI IFNYNNSSSS NNNNDNNNNN NSNSASGSGG AGAAAAAATA 450
    PENEDGNGYD TNIKTLINDG VLNKDDVKRV LNNLIVSHNK 490
    Length:490
    Mass (Da):54,382
    Last modified:October 1, 1996 - v1
    Checksum:i4D0DA8DE43F171ED
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U25279 Genomic DNA. Translation: AAC49982.1.
    Z48952 Genomic DNA. Translation: CAA88795.1.
    AY693209 Genomic DNA. Translation: AAT93228.1.
    BK006946 Genomic DNA. Translation: DAA09968.1.
    PIRiS52830.
    RefSeqiNP_013786.1. NM_001182568.1.

    Genome annotation databases

    EnsemblFungiiYMR070W; YMR070W; YMR070W.
    GeneIDi855092.
    KEGGisce:YMR070W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U25279 Genomic DNA. Translation: AAC49982.1 .
    Z48952 Genomic DNA. Translation: CAA88795.1 .
    AY693209 Genomic DNA. Translation: AAT93228.1 .
    BK006946 Genomic DNA. Translation: DAA09968.1 .
    PIRi S52830.
    RefSeqi NP_013786.1. NM_001182568.1.

    3D structure databases

    ProteinModelPortali P54785.
    SMRi P54785. Positions 339-432.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35245. 115 interactions.
    IntActi P54785. 26 interactions.
    MINTi MINT-4497702.
    STRINGi 4932.YMR070W.

    Proteomic databases

    MaxQBi P54785.
    PaxDbi P54785.
    PeptideAtlasi P54785.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR070W ; YMR070W ; YMR070W .
    GeneIDi 855092.
    KEGGi sce:YMR070W.

    Organism-specific databases

    CYGDi YMR070w.
    SGDi S000004674. MOT3.

    Phylogenomic databases

    eggNOGi NOG255610.
    OMAi ASYISHY.
    OrthoDBi EOG757D9C.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32772-MONOMER.

    Miscellaneous databases

    NextBioi 978399.

    Gene expression databases

    Genevestigatori P54785.

    Family and domain databases

    Gene3Di 3.30.160.60. 2 hits.
    InterProi IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view ]
    Pfami PF00096. zf-C2H2. 1 hit.
    [Graphical view ]
    SMARTi SM00355. ZnF_C2H2. 2 hits.
    [Graphical view ]
    PROSITEi PS00028. ZINC_FINGER_C2H2_1. 1 hit.
    PS50157. ZINC_FINGER_C2H2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and analysis of Mot3, a zinc finger protein that binds to the retrotransposon Ty long terminal repeat (delta) in Saccharomyces cerevisiae."
      Madison J.M., Dudley A.M., Winston F.
      Mol. Cell. Biol. 18:1879-1890(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DNA-BINDING.
      Strain: ATCC 204508 / S288c.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Mot3, a Zn finger transcription factor that modulates gene expression and attenuates mating pheromone signaling in Saccharomyces cerevisiae."
      Grishin A.V., Rothenberg M., Downs M.A., Blumer K.J.
      Genetics 149:879-892(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN TRANSCRIPTIONAL ACTIVATION, DNA-BINDING, SUBCELLULAR LOCATION.
    6. "Roles of transcription factor Mot3 and chromatin in repression of the hypoxic gene ANB1 in yeast."
      Kastaniotis A.J., Mennella T.A., Konrad C., Rodriguez Torres A.M., Zitomer R.S.
      Mol. Cell. Biol. 20:7088-7098(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Mot3 is a transcriptional repressor of ergosterol biosynthetic genes and is required for normal vacuolar function in Saccharomyces cerevisiae."
      Hongay C., Jia N., Bard M., Winston F.
      EMBO J. 21:4114-4124(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN TRANSCRIPTIONAL REPRESSION.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Synergistic repression of anaerobic genes by Mot3 and Rox1 in Saccharomyces cerevisiae."
      Sertil O., Kapoor R., Cohen B.D., Abramova N., Lowry C.V.
      Nucleic Acids Res. 31:5831-5837(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN REPRESSION OF HYPOXIC GENES, INDUCTION.
    10. "Combinatorial repression of the hypoxic genes of Saccharomyces cerevisiae by DNA binding proteins Rox1 and Mot3."
      Klinkenberg L.G., Mennella T.A., Luetkenhaus K., Zitomer R.S.
      Eukaryot. Cell 4:649-660(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. "A systematic survey identifies prions and illuminates sequence features of prionogenic proteins."
      Alberti S., Halfmann R., King O., Kapila A., Lindquist S.
      Cell 137:146-158(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PRION FORMATION.
    12. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMOT3_YEAST
    AccessioniPrimary (citable) accession number: P54785
    Secondary accession number(s): D6VZP4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 125 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    [MOT3+] is the prion form of MOT3. [MOT3+] is the result of a conformational change of the cellular MOT3 protein that becomes self-propagating and infectious. This conformational change generates a form of MOT3 that assembles into amyloid fibrils. [MOT3+] aggregates sequester soluble MOT3, resulting in a loss-of-function phenotype for MOT3. [MOT3+] can be cured by GdnHCl and by inactivation of the molecular chaperone HSP104, which is required for [MOT3+] propagation. It is speculated that prion properties of transcription factors may generate an optimized phenotypic heterogeneity that buffers yeast populations against diverse environmental insults (PubMed:19345193).1 Publication
    Present with 1690 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3