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P54785

- MOT3_YEAST

UniProt

P54785 - MOT3_YEAST

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Protein

Transcriptional activator/repressor MOT3

Gene

MOT3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Transcription factor that affects the expression of a large set of genes. Recognizes and binds to the consensus sequence 5'-[CAT]AGG[TC]A-3' in the promoter region. Plays a major role in the repression of a specific subset of hypoxic genes (e.g. ANB1, DAN1 and HEM13) under aerobic conditions. Acts synergistically with the transcription factor ROX1 to recruit the general repression complex SSN6-TUP1 to the promoter of hypoxic genes. Represses transcription of ergosterol biosynthetic genes. Negatively regulates pheromone-induced gene expression. Can act as a transcriptional activator (e.g. of genes like CYC1, SUC2 and the Ty long terminal repeat delta promoter).6 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri346 – 36823C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri374 – 39724C2H2-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. RNA polymerase II activating transcription factor binding Source: SGD
  3. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: SGD
  4. RNA polymerase II repressing transcription factor binding Source: SGD

GO - Biological processi

  1. cellular hyperosmotic response Source: SGD
  2. negative regulation of ergosterol biosynthetic process Source: SGD
  3. negative regulation of transcription from RNA polymerase II promoter Source: SGD
  4. positive regulation of transcription from RNA polymerase II promoter Source: SGD
  5. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Prion, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32772-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional activator/repressor MOT3
Alternative name(s):
Hypoxic gene repressor protein 7
Modulator of transcription protein 3
Gene namesi
Name:MOT3
Synonyms:ROX7
Ordered Locus Names:YMR070W
ORF Names:YM9916.09
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR070w.
SGDiS000004674. MOT3.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. cytosol Source: SGD
  2. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Amyloid, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 490490Transcriptional activator/repressor MOT3PRO_0000046807Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP54785.
PaxDbiP54785.
PeptideAtlasiP54785.

Expressioni

Inductioni

Induced under aerobic conditions and repressed under anaerobic conditions.1 Publication

Gene expression databases

GenevestigatoriP54785.

Interactioni

Protein-protein interaction databases

BioGridi35245. 115 interactions.
IntActiP54785. 26 interactions.
MINTiMINT-4497702.
STRINGi4932.YMR070W.

Structurei

3D structure databases

ProteinModelPortaliP54785.
SMRiP54785. Positions 344-399.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni98 – 295198Prion domain (PrD)Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi8 – 3528Poly-GlnAdd
BLAST
Compositional biasi98 – 1047Poly-Asn
Compositional biasi143 – 15715Poly-AsnAdd
BLAST
Compositional biasi173 – 1775Poly-Ala
Compositional biasi240 – 2456Poly-His
Compositional biasi417 – 4204Poly-Ser
Compositional biasi421 – 43313Poly-AsnAdd
BLAST
Compositional biasi441 – 45010Poly-Ala

Domaini

The prion domain (PrD) is a Gln/Asn (Q/N)-rich domain, which is unstructured in its native, soluble form, and which forms a parallel in-register beta-sheet in its amyloid form.By similarity

Sequence similaritiesi

Contains 2 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri346 – 36823C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri374 – 39724C2H2-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG255610.
InParanoidiP54785.
OMAiASYISHY.
OrthoDBiEOG757D9C.

Family and domain databases

Gene3Di3.30.160.60. 2 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 2 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54785-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNADHHLQQQ QQQRQQHQQQ QHQQQQHQHQ HQQQQHTILQ NVSNTNNIGS
60 70 80 90 100
DSLASQPFNT TTVSSNKDDV MVNSGARELP MPLHQQQYIY PYYQYTSNNS
110 120 130 140 150
NNNNVTAGNN MSASPIVHNN SNNSNNSNIS ASDYTVANNS TSNNNNNNNN
160 170 180 190 200
NNNNNNNIHP NQFTAAANMN SNAAAAAYYS FPTANMPIPQ QDQQYMFNPA
210 220 230 240 250
SYISHYYSAV NSNNNGNNAA NNGSNNSSHS APAPAPGPPH HHHHHSNTHN
260 270 280 290 300
NLNNGGAVNT NNAPQHHPTI ITDQFQFQLQ QNPSPNLNLN INPAQPLHLP
310 320 330 340 350
PGWKINTMPQ PRPTTAPNHP PAPVPSSNPV ASNLVPAPSS DHKYIHQCQF
360 370 380 390 400
CEKSFKRKSW LKRHLLSHSQ QRHFLCPWCL SRQKRKDNLL QHMKLKHTNY
410 420 430 440 450
LLDELKKNNI IFNYNNSSSS NNNNDNNNNN NSNSASGSGG AGAAAAAATA
460 470 480 490
PENEDGNGYD TNIKTLINDG VLNKDDVKRV LNNLIVSHNK
Length:490
Mass (Da):54,382
Last modified:October 1, 1996 - v1
Checksum:i4D0DA8DE43F171ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25279 Genomic DNA. Translation: AAC49982.1.
Z48952 Genomic DNA. Translation: CAA88795.1.
AY693209 Genomic DNA. Translation: AAT93228.1.
BK006946 Genomic DNA. Translation: DAA09968.1.
PIRiS52830.
RefSeqiNP_013786.1. NM_001182568.1.

Genome annotation databases

EnsemblFungiiYMR070W; YMR070W; YMR070W.
GeneIDi855092.
KEGGisce:YMR070W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25279 Genomic DNA. Translation: AAC49982.1 .
Z48952 Genomic DNA. Translation: CAA88795.1 .
AY693209 Genomic DNA. Translation: AAT93228.1 .
BK006946 Genomic DNA. Translation: DAA09968.1 .
PIRi S52830.
RefSeqi NP_013786.1. NM_001182568.1.

3D structure databases

ProteinModelPortali P54785.
SMRi P54785. Positions 344-399.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35245. 115 interactions.
IntActi P54785. 26 interactions.
MINTi MINT-4497702.
STRINGi 4932.YMR070W.

Proteomic databases

MaxQBi P54785.
PaxDbi P54785.
PeptideAtlasi P54785.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR070W ; YMR070W ; YMR070W .
GeneIDi 855092.
KEGGi sce:YMR070W.

Organism-specific databases

CYGDi YMR070w.
SGDi S000004674. MOT3.

Phylogenomic databases

eggNOGi NOG255610.
InParanoidi P54785.
OMAi ASYISHY.
OrthoDBi EOG757D9C.

Enzyme and pathway databases

BioCyci YEAST:G3O-32772-MONOMER.

Miscellaneous databases

NextBioi 978399.

Gene expression databases

Genevestigatori P54785.

Family and domain databases

Gene3Di 3.30.160.60. 2 hits.
InterProi IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view ]
Pfami PF00096. zf-C2H2. 1 hit.
[Graphical view ]
SMARTi SM00355. ZnF_C2H2. 2 hits.
[Graphical view ]
PROSITEi PS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and analysis of Mot3, a zinc finger protein that binds to the retrotransposon Ty long terminal repeat (delta) in Saccharomyces cerevisiae."
    Madison J.M., Dudley A.M., Winston F.
    Mol. Cell. Biol. 18:1879-1890(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DNA-BINDING.
    Strain: ATCC 204508 / S288c.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Mot3, a Zn finger transcription factor that modulates gene expression and attenuates mating pheromone signaling in Saccharomyces cerevisiae."
    Grishin A.V., Rothenberg M., Downs M.A., Blumer K.J.
    Genetics 149:879-892(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN TRANSCRIPTIONAL ACTIVATION, DNA-BINDING, SUBCELLULAR LOCATION.
  6. "Roles of transcription factor Mot3 and chromatin in repression of the hypoxic gene ANB1 in yeast."
    Kastaniotis A.J., Mennella T.A., Konrad C., Rodriguez Torres A.M., Zitomer R.S.
    Mol. Cell. Biol. 20:7088-7098(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Mot3 is a transcriptional repressor of ergosterol biosynthetic genes and is required for normal vacuolar function in Saccharomyces cerevisiae."
    Hongay C., Jia N., Bard M., Winston F.
    EMBO J. 21:4114-4124(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN TRANSCRIPTIONAL REPRESSION.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Synergistic repression of anaerobic genes by Mot3 and Rox1 in Saccharomyces cerevisiae."
    Sertil O., Kapoor R., Cohen B.D., Abramova N., Lowry C.V.
    Nucleic Acids Res. 31:5831-5837(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN REPRESSION OF HYPOXIC GENES, INDUCTION.
  10. "Combinatorial repression of the hypoxic genes of Saccharomyces cerevisiae by DNA binding proteins Rox1 and Mot3."
    Klinkenberg L.G., Mennella T.A., Luetkenhaus K., Zitomer R.S.
    Eukaryot. Cell 4:649-660(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "A systematic survey identifies prions and illuminates sequence features of prionogenic proteins."
    Alberti S., Halfmann R., King O., Kapila A., Lindquist S.
    Cell 137:146-158(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRION FORMATION.
  12. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMOT3_YEAST
AccessioniPrimary (citable) accession number: P54785
Secondary accession number(s): D6VZP4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

[MOT3+] is the prion form of MOT3. [MOT3+] is the result of a conformational change of the cellular MOT3 protein that becomes self-propagating and infectious. This conformational change generates a form of MOT3 that assembles into amyloid fibrils. [MOT3+] aggregates sequester soluble MOT3, resulting in a loss-of-function phenotype for MOT3. [MOT3+] can be cured by GdnHCl and by inactivation of the molecular chaperone HSP104, which is required for [MOT3+] propagation. It is speculated that prion properties of transcription factors may generate an optimized phenotypic heterogeneity that buffers yeast populations against diverse environmental insults (PubMed:19345193).1 Publication
Present with 1690 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3